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The map label for this gene is murD [H]

Identifier: 169629091

GI number: 169629091

Start: 2002833

End: 2004299

Strand: Direct

Name: murD [H]

Synonym: MAB_2004

Alternate gene names: 169629091

Gene position: 2002833-2004299 (Clockwise)

Preceding gene: 169629090

Following gene: 169629092

Centisome position: 39.53

GC content: 67.48

Gene sequence:

>1467_bases
ATGACCACGTGTGAAGTCGCCGAACTGACCGGCGGAACAAGGGTTCTGGTGTGCGGTGCGCGGGTCACCGGCGACGCCGT
GGTCGCGGCGTTGACCGGGCTAGCCGACCTCGGTCTGCGCATTACGGTCTGCGACGACGATGAGGACGCCCTGCAACGCC
ATCGTGATGCCGGGCACGGGGCACTGACCACAGCAGCAGCGCAACGTCACATGGCCGATCAGGACCTGGTGATCGTCAGC
CCCGGATTCGGCCCCTCGGCACCGGTGGTGCAGGCCGCGGTACAGGCCGGGCTGCCCATTTGGGGTGACGTCGAATTGGC
TTGGCGTTTGGACGCTTCCGGTAGGTACGGGCGGCCCAAGCGGTGGGTGGTGGTCACCGGGACCAACGGGAAGACCACGA
CCACGGCCATGGTCCACGACATGCTGACGGCGGCCGGTGGCACCAGTGCGTTGTGCGGGAATATCGGCGATCCGGTGCTC
AACGTGCTGGATCGTGACGTCGAGTTCCTGGCCGTCGAGTTGTCCAGCTTTCAGCTGCACTGGGCGCCGTCGGTGCGACC
GGTGGCCGGCGCGGTGCTCAACGTGGCCGAGGATCACCTCGATTGGCATGGGGGCATGGAGGGCTACACCCGGGACAAGG
CCCGGGCGCTCGATGGCGAGGTCGCCGTTGCCGGTCTAGATGATGCGATCGCCGGGGGTCTGCTTGCCGCTGCCGCGGCA
CCGGTCAAGGCGGGATTTCGTGCCGGGGAGCCCGCGGTGGGCGAGCTGGGGGTGCTGGACGGTTGGTTGGTGGATCGGGC
ATTCGGTGTCGACATACCGCTGATCGAGGTGTCGAAGCTGCCGGTGGGGGGGCCGATCGGCGTGCTCAACGCGCTGGCGG
CCGCCGCGCTGGCCCGTGCCGTAGGTACACCTGCGGTGCACATTGCCCGGGCGCTGGAAGTTTTTCAGGTGGGGCGGCAT
CGGGCCGAGCTGATTGCCCGCGTCGCCGATGTTGCCTACGTCGACGACTCGAAGGCCACCAATCCCCATGCTGCCGCCGC
GTCCATCGCGGGGTATCCGCGTGTCGTCTGGATTGCTGGAGGGCTGCTCAAGGGAGCTGCGGTCGATGATTTGGTGCGCG
ATTGTGCCGACCGGCTAGGTGCGGTTGTGCTGATCGGCCGGGATCGGGCACTGATTGCTGAGGCTTTGGCGCGACACGCG
CCGGATGTCCCCGTAGTTGCGGTTGTGACGCGCGAGGATGCTGGGGTGCAAGAGGCAAATGTGGTTTATGTGACTGATGA
GGCGGATGGGGGTGGCGCTGACCTGGGTACCCGAGTCATGGCCGCGGCCGTCCGGCACGCCAGCTCACTGGCCCGGCCTG
GCGACACCGTGCTTCTGGCTCCGGCAGGCGCGTCCTTCGACCAATTCAGCGGGTATTCCGAGCGTGGCGACCGGTTCGCC
GCGGCGGTGCACGCACTTACCGGTTAG

Upstream 100 bases:

>100_bases
CGTCATCATTCGGTTCTGGTTACTGACCGCCATCGCCTGTGGCTTGGGCTTGGCACTGTTCTATGGCGAGTGGCTCGCCA
CGATCGGTGACTAGGCGACC

Downstream 100 bases:

>100_bases
GTGATTGCGTCGTGTCCACCCTGACTGCTCCATTCGCCAGGATTTTCCGTTCCGAGAAGCGCGACGAAGCAAAGACCTCC
GTCACGTCGGTCAAGAAGTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTTCEVAELTGGTRVLVCGARVTGDAVVAALTGLADLGLRITVCDDDEDALQRHRDAGHGALTTAAAQRHMADQDLVIVS
PGFGPSAPVVQAAVQAGLPIWGDVELAWRLDASGRYGRPKRWVVVTGTNGKTTTTAMVHDMLTAAGGTSALCGNIGDPVL
NVLDRDVEFLAVELSSFQLHWAPSVRPVAGAVLNVAEDHLDWHGGMEGYTRDKARALDGEVAVAGLDDAIAGGLLAAAAA
PVKAGFRAGEPAVGELGVLDGWLVDRAFGVDIPLIEVSKLPVGGPIGVLNALAAAALARAVGTPAVHIARALEVFQVGRH
RAELIARVADVAYVDDSKATNPHAAAASIAGYPRVVWIAGGLLKGAAVDDLVRDCADRLGAVVLIGRDRALIAEALARHA
PDVPVVAVVTREDAGVQEANVVYVTDEADGGGADLGTRVMAAAVRHASSLARPGDTVLLAPAGASFDQFSGYSERGDRFA
AAVHALTG

Sequences:

>Translated_488_residues
MTTCEVAELTGGTRVLVCGARVTGDAVVAALTGLADLGLRITVCDDDEDALQRHRDAGHGALTTAAAQRHMADQDLVIVS
PGFGPSAPVVQAAVQAGLPIWGDVELAWRLDASGRYGRPKRWVVVTGTNGKTTTTAMVHDMLTAAGGTSALCGNIGDPVL
NVLDRDVEFLAVELSSFQLHWAPSVRPVAGAVLNVAEDHLDWHGGMEGYTRDKARALDGEVAVAGLDDAIAGGLLAAAAA
PVKAGFRAGEPAVGELGVLDGWLVDRAFGVDIPLIEVSKLPVGGPIGVLNALAAAALARAVGTPAVHIARALEVFQVGRH
RAELIARVADVAYVDDSKATNPHAAAASIAGYPRVVWIAGGLLKGAAVDDLVRDCADRLGAVVLIGRDRALIAEALARHA
PDVPVVAVVTREDAGVQEANVVYVTDEADGGGADLGTRVMAAAVRHASSLARPGDTVLLAPAGASFDQFSGYSERGDRFA
AAVHALTG
>Mature_487_residues
TTCEVAELTGGTRVLVCGARVTGDAVVAALTGLADLGLRITVCDDDEDALQRHRDAGHGALTTAAAQRHMADQDLVIVSP
GFGPSAPVVQAAVQAGLPIWGDVELAWRLDASGRYGRPKRWVVVTGTNGKTTTTAMVHDMLTAAGGTSALCGNIGDPVLN
VLDRDVEFLAVELSSFQLHWAPSVRPVAGAVLNVAEDHLDWHGGMEGYTRDKARALDGEVAVAGLDDAIAGGLLAAAAAP
VKAGFRAGEPAVGELGVLDGWLVDRAFGVDIPLIEVSKLPVGGPIGVLNALAAAALARAVGTPAVHIARALEVFQVGRHR
AELIARVADVAYVDDSKATNPHAAAASIAGYPRVVWIAGGLLKGAAVDDLVRDCADRLGAVVLIGRDRALIAEALARHAP
DVPVVAVVTREDAGVQEANVVYVTDEADGGGADLGTRVMAAAVRHASSLARPGDTVLLAPAGASFDQFSGYSERGDRFAA
AVHALTG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=412, Percent_Identity=33.0097087378641, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50032; Mature: 49901

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTCEVAELTGGTRVLVCGARVTGDAVVAALTGLADLGLRITVCDDDEDALQRHRDAGHG
CCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCC
ALTTAAAQRHMADQDLVIVSPGFGPSAPVVQAAVQAGLPIWGDVELAWRLDASGRYGRPK
HHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCC
RWVVVTGTNGKTTTTAMVHDMLTAAGGTSALCGNIGDPVLNVLDRDVEFLAVELSSFQLH
EEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCEEEEEEEECEEEE
WAPSVRPVAGAVLNVAEDHLDWHGGMEGYTRDKARALDGEVAVAGLDDAIAGGLLAAAAA
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHH
PVKAGFRAGEPAVGELGVLDGWLVDRAFGVDIPLIEVSKLPVGGPIGVLNALAAAALARA
HHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
VGTPAVHIARALEVFQVGRHRAELIARVADVAYVDDSKATNPHAAAASIAGYPRVVWIAG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCCEEEEEEC
GLLKGAAVDDLVRDCADRLGAVVLIGRDRALIAEALARHAPDVPVVAVVTREDAGVQEAN
CHHCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
VVYVTDEADGGGADLGTRVMAAAVRHASSLARPGDTVLLAPAGASFDQFSGYSERGDRFA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCHHHCHHHH
AAVHALTG
HHHHHHCC
>Mature Secondary Structure 
TTCEVAELTGGTRVLVCGARVTGDAVVAALTGLADLGLRITVCDDDEDALQRHRDAGHG
CCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCC
ALTTAAAQRHMADQDLVIVSPGFGPSAPVVQAAVQAGLPIWGDVELAWRLDASGRYGRPK
HHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCC
RWVVVTGTNGKTTTTAMVHDMLTAAGGTSALCGNIGDPVLNVLDRDVEFLAVELSSFQLH
EEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCEEEEEEEECEEEE
WAPSVRPVAGAVLNVAEDHLDWHGGMEGYTRDKARALDGEVAVAGLDDAIAGGLLAAAAA
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHH
PVKAGFRAGEPAVGELGVLDGWLVDRAFGVDIPLIEVSKLPVGGPIGVLNALAAAALARA
HHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
VGTPAVHIARALEVFQVGRHRAELIARVADVAYVDDSKATNPHAAAASIAGYPRVVWIAG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCCEEEEEEC
GLLKGAAVDDLVRDCADRLGAVVLIGRDRALIAEALARHAPDVPVVAVVTREDAGVQEAN
CHHCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
VVYVTDEADGGGADLGTRVMAAAVRHASSLARPGDTVLLAPAGASFDQFSGYSERGDRFA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCHHHCHHHH
AAVHALTG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA