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The map label for this gene is murF [H]

Identifier: 169629089

GI number: 169629089

Start: 2000275

End: 2001750

Strand: Direct

Name: murF [H]

Synonym: MAB_2002

Alternate gene names: 169629089

Gene position: 2000275-2001750 (Clockwise)

Preceding gene: 169629088

Following gene: 169629090

Centisome position: 39.48

GC content: 69.92

Gene sequence:

>1476_bases
ATGACTGTGGCCGAGATCGCCGAAATCGTCGGTGGGCGCCTTGACGGAATCAGCGCGCAGGAAGCTCGGCAGGCGCGCGT
CACCGGCACCGTGGAATTCGATTCACGTCAGGTGAGTCCCGGCGGTCTGTTCTTGGCCCTGCCGGGAGCCCGGGCGGATG
GCCACGACCACGCCCGAGGTGCCGCGGAGGCCGGGGCGGTCGCGGTGCTGGCCGCCAGGCCGGTGGGCGTACCCGCCATC
GTGGTGCCGCCGACCGGGGTGGCACGTACCGAAGTATCTGCGCTGGAACATGATTCGGATGGGTCTGGCGCAGCGGTGCT
CGCCGCGACGGCAGCGCTCGCGGCCGAGGTCGCCCGTCGGCTGACGGCGCGGGGGCTACGGATCGTCGGGATCACGGGCT
CGTCGGGCAAGACCTCGACAAAGGATTTGATCGCCGCCGTGCTCAGCCCGCTCGGCGAGGTGGTGGCTCCTCCCGGGTCG
TTCAACAACGAGCTCGGCCATCCCTGGACGGTGCTGCGGGCGACGGAATCCACCAAGTTCTTGGTGCTGGAGCTTTCGGC
CCGGCGTCCGGGCAACATCGCGGAATTGGCTGCCATCGCGCCGCCGTCGATCGGGGTCGTTCTCAACGTCGGTACCGCTC
ATCTGGGTGAGTTCGGGTCGCGGCAGGCCATCGCCGATACGAAGGGGGAACTGCCGCAATCGCTTTCCGGTGGCGGCGTG
GCGGTGTTGAACGCCGATGATGCCGCGGTGGCGGCGATGGCGGCCAAGACTCCCGCCCGCGTGGTACGCACCAGCGTGCA
CCATCCCGCCGATATCTGGGCGACCGAGGTGAGCGTCGACGACCTGGCCCGGGCCCGTTTCACGCTGCACGCCGGGCAAG
ACTCGGTGCCCGTGCAACTGGCGGTGCACGGCGAGCACCACGTGAGCAACGCGCTGGCCGCCGCCGCCGTGGCGCTGGAA
TGCGGCGCCACCCTCGCACAGGTGGCGGCGGCGCTCGCCGGGGCGGGGCCGGTCTCTCGCCGCCGCATGGAGGTCAGCAC
CCGAGATGACGGGGTGACCGTGATCAACGATGCCTACAACGCCAACCCCGACTCGATGATGGCGGGCCTGAAAGCCCTGG
TCTCGATGGCGCGGCACGGGGGCGAGCCCCGTCGCAGCTGGGCGGTGCTGGGGGAGATGGCCGAGCTGGGACCCGATGCG
ATATCCGAGCATGACCGCATCGGGCGGGCCGCCGTGCGATTAGATGTCAGTCGATTGGTAGTCGTCGGGACTGGGAGGTC
ACAGCGGGCAATGCATGCGGGTGCGGTGATGGAGGGGTCGTGGGGGTCCGAGGCGGTGCTGGTGGACGATCCTGCGGCGG
CCGCCTCACTGCTGGCCAGTGAGCTGGCCGCGGGCGATGTGGTGCTGGTCAAGGCGTCACGGTCGGCGGGCCTCTGGGTG
GTGGCCGATGAACTGCTGAAGGTGGGTGACGCATGA

Upstream 100 bases:

>100_bases
CATGAGAGCGGTCAGAAGACCGGCGATGTCACGGTGCCTTTCGATGATCGTGTGGAGTTGCGCGCAGCCCTCGAAGCGCT
TGGGAGCCTGGATGATTGAG

Downstream 100 bases:

>100_bases
GGCAGATCATCATTGCCGCCGGTATCGCGATCCTGGTCTCGATCATGCTCACCCCGGTACTCATCCGGGTGTTTTCACGG
CAGGGATTCGGCCAGGAGAT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase MurF

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTVAEIAEIVGGRLDGISAQEARQARVTGTVEFDSRQVSPGGLFLALPGARADGHDHARGAAEAGAVAVLAARPVGVPAI
VVPPTGVARTEVSALEHDSDGSGAAVLAATAALAAEVARRLTARGLRIVGITGSSGKTSTKDLIAAVLSPLGEVVAPPGS
FNNELGHPWTVLRATESTKFLVLELSARRPGNIAELAAIAPPSIGVVLNVGTAHLGEFGSRQAIADTKGELPQSLSGGGV
AVLNADDAAVAAMAAKTPARVVRTSVHHPADIWATEVSVDDLARARFTLHAGQDSVPVQLAVHGEHHVSNALAAAAVALE
CGATLAQVAAALAGAGPVSRRRMEVSTRDDGVTVINDAYNANPDSMMAGLKALVSMARHGGEPRRSWAVLGEMAELGPDA
ISEHDRIGRAAVRLDVSRLVVVGTGRSQRAMHAGAVMEGSWGSEAVLVDDPAAAASLLASELAAGDVVLVKASRSAGLWV
VADELLKVGDA

Sequences:

>Translated_491_residues
MTVAEIAEIVGGRLDGISAQEARQARVTGTVEFDSRQVSPGGLFLALPGARADGHDHARGAAEAGAVAVLAARPVGVPAI
VVPPTGVARTEVSALEHDSDGSGAAVLAATAALAAEVARRLTARGLRIVGITGSSGKTSTKDLIAAVLSPLGEVVAPPGS
FNNELGHPWTVLRATESTKFLVLELSARRPGNIAELAAIAPPSIGVVLNVGTAHLGEFGSRQAIADTKGELPQSLSGGGV
AVLNADDAAVAAMAAKTPARVVRTSVHHPADIWATEVSVDDLARARFTLHAGQDSVPVQLAVHGEHHVSNALAAAAVALE
CGATLAQVAAALAGAGPVSRRRMEVSTRDDGVTVINDAYNANPDSMMAGLKALVSMARHGGEPRRSWAVLGEMAELGPDA
ISEHDRIGRAAVRLDVSRLVVVGTGRSQRAMHAGAVMEGSWGSEAVLVDDPAAAASLLASELAAGDVVLVKASRSAGLWV
VADELLKVGDA
>Mature_490_residues
TVAEIAEIVGGRLDGISAQEARQARVTGTVEFDSRQVSPGGLFLALPGARADGHDHARGAAEAGAVAVLAARPVGVPAIV
VPPTGVARTEVSALEHDSDGSGAAVLAATAALAAEVARRLTARGLRIVGITGSSGKTSTKDLIAAVLSPLGEVVAPPGSF
NNELGHPWTVLRATESTKFLVLELSARRPGNIAELAAIAPPSIGVVLNVGTAHLGEFGSRQAIADTKGELPQSLSGGGVA
VLNADDAAVAAMAAKTPARVVRTSVHHPADIWATEVSVDDLARARFTLHAGQDSVPVQLAVHGEHHVSNALAAAAVALEC
GATLAQVAAALAGAGPVSRRRMEVSTRDDGVTVINDAYNANPDSMMAGLKALVSMARHGGEPRRSWAVLGEMAELGPDAI
SEHDRIGRAAVRLDVSRLVVVGTGRSQRAMHAGAVMEGSWGSEAVLVDDPAAAASLLASELAAGDVVLVKASRSAGLWVV
ADELLKVGDA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=485, Percent_Identity=33.6082474226804, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49777; Mature: 49646

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAEIAEIVGGRLDGISAQEARQARVTGTVEFDSRQVSPGGLFLALPGARADGHDHARG
CCHHHHHHHHCCCCCCCCHHHHHHEEEEEEEEECCEECCCCEEEEEECCCCCCCCCHHCC
AAEAGAVAVLAARPVGVPAIVVPPTGVARTEVSALEHDSDGSGAAVLAATAALAAEVARR
CCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LTARGLRIVGITGSSGKTSTKDLIAAVLSPLGEVVAPPGSFNNELGHPWTVLRATESTKF
HHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEE
LVLELSARRPGNIAELAAIAPPSIGVVLNVGTAHLGEFGSRQAIADTKGELPQSLSGGGV
EEEEECCCCCCCHHHHHEECCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCE
AVLNADDAAVAAMAAKTPARVVRTSVHHPADIWATEVSVDDLARARFTLHAGQDSVPVQL
EEEECCCHHHHHHHHCCHHHHHHHHCCCCCHHEEEECCHHHHHHHEEEEECCCCCCCEEE
AVHGEHHVSNALAAAAVALECGATLAQVAAALAGAGPVSRRRMEVSTRDDGVTVINDAYN
EECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHEEEEECCCCCEEEEECCCC
ANPDSMMAGLKALVSMARHGGEPRRSWAVLGEMAELGPDAISEHDRIGRAAVRLDVSRLV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHEEEEEEEEE
VVGTGRSQRAMHAGAVMEGSWGSEAVLVDDPAAAASLLASELAAGDVVLVKASRSAGLWV
EEECCCCCHHHHCCCEEECCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCEEE
VADELLKVGDA
EEHHHHHCCCC
>Mature Secondary Structure 
TVAEIAEIVGGRLDGISAQEARQARVTGTVEFDSRQVSPGGLFLALPGARADGHDHARG
CHHHHHHHHCCCCCCCCHHHHHHEEEEEEEEECCEECCCCEEEEEECCCCCCCCCHHCC
AAEAGAVAVLAARPVGVPAIVVPPTGVARTEVSALEHDSDGSGAAVLAATAALAAEVARR
CCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LTARGLRIVGITGSSGKTSTKDLIAAVLSPLGEVVAPPGSFNNELGHPWTVLRATESTKF
HHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEE
LVLELSARRPGNIAELAAIAPPSIGVVLNVGTAHLGEFGSRQAIADTKGELPQSLSGGGV
EEEEECCCCCCCHHHHHEECCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCE
AVLNADDAAVAAMAAKTPARVVRTSVHHPADIWATEVSVDDLARARFTLHAGQDSVPVQL
EEEECCCHHHHHHHHCCHHHHHHHHCCCCCHHEEEECCHHHHHHHEEEEECCCCCCCEEE
AVHGEHHVSNALAAAAVALECGATLAQVAAALAGAGPVSRRRMEVSTRDDGVTVINDAYN
EECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHEEEEECCCCCEEEEECCCC
ANPDSMMAGLKALVSMARHGGEPRRSWAVLGEMAELGPDAISEHDRIGRAAVRLDVSRLV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHEEEEEEEEE
VVGTGRSQRAMHAGAVMEGSWGSEAVLVDDPAAAASLLASELAAGDVVLVKASRSAGLWV
EEECCCCCHHHHCCCEEECCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCEEE
VADELLKVGDA
EEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]