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The map label for this gene is ispE [H]

Identifier: 169628232

GI number: 169628232

Start: 1154427

End: 1155371

Strand: Direct

Name: ispE [H]

Synonym: MAB_1139

Alternate gene names: 169628232

Gene position: 1154427-1155371 (Clockwise)

Preceding gene: 169628229

Following gene: 169628233

Centisome position: 22.78

GC content: 68.89

Gene sequence:

>945_bases
GTGTCCGAGACCGTCTCCGATTGGGTTCCTACCGGCGCGGTGACTGTCCGGGCACCCGGCAAGGTCAATCTGTACCTGGC
CGTAGGTGACCTCCGTGACGACGGCTATCACGAGCTGACCACGGTTTTTCACGCCGTTTCGCTGGCCGACGACGTGACCG
TGCGGGACGCCGACGTGTTATCGATCGATGTGGTCGGGCAGGGCGAGGGCACGGTGCCCACCGACGAACGCAATCTCGCC
TGGCAGGCCGCCGAGCTTTTCGCCGATCACGTGGGCCGCGCCCCCGACGTCTCGATCTTCATTAACAAGGACATCCCCGT
CGCGGGCGGTATGGCCGGCGGATCCGCCGATGCCGCTGCGGTGTTGGTGGCGATGAACGAGCTCTGGCATGCCGGTGTGC
CGCGCCGGGACCTGCACCACCTGGCCGCTCAACTGGGCAGCGATGTCCCCTTTGCCTTACATGGCGGCACCGCGCTGGGC
ACTGGCCGGGGTGAGCAGCTGGCGACCGTATTGGCGCGCAATGTCTTTCATTGGGTGTTCGCCTTCGCCGACGGGGGACT
GGCGACGCCTCAGGTCTTCAAGGAGATCGACCGGTTGCGTGAGAACGGCGATCCGCCGCGGCTCGCCGAGGCGGATGAGC
TTTTGGGTGCGCTCGCCGCCGGGGACGCGCGCCGGCTGGCGCCGCTGCTCGGTAACGAATTGCAGGCCGCGGCGGTCAGC
CTGAACCCCGAATTGCGGCGCACGCTGCGGGCAGGGGAGTCGGCCGGCGCCCTGGCTGGAATCGTCTCCGGATCGGGCCC
CACCTGTGCGTTCTTGTGCACCTCGGCCGACGATGCGGTGCAGGTGAGTGCGGAGCTGGCGGGCGCGGGGGTGTGCCGCA
CCGTGCGGGTGGCCAGCGGACCCGTGCACGGCGCACAGGTCATCCAGGGCCGCAGTGACGGCTGA

Upstream 100 bases:

>100_bases
GGCATCGGTCATTTCCCTGACTTTAGTCAGAGAAGGCCTCGATGTCTGCGTTGTGTGATGGATGAGCGCCATTCGCGCCG
CAGAACGTACCCTCAGGGGC

Downstream 100 bases:

>100_bases
CCCGACACGCCCGCGACCGCGACACGCCGAATACCAAGATCACGGTGGGTTTGGCCGATGATTAAGAAAAGCCTAAGATG
CTAGCGGATTGTTAGAAGGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSETVSDWVPTGAVTVRAPGKVNLYLAVGDLRDDGYHELTTVFHAVSLADDVTVRDADVLSIDVVGQGEGTVPTDERNLA
WQAAELFADHVGRAPDVSIFINKDIPVAGGMAGGSADAAAVLVAMNELWHAGVPRRDLHHLAAQLGSDVPFALHGGTALG
TGRGEQLATVLARNVFHWVFAFADGGLATPQVFKEIDRLRENGDPPRLAEADELLGALAAGDARRLAPLLGNELQAAAVS
LNPELRRTLRAGESAGALAGIVSGSGPTCAFLCTSADDAVQVSAELAGAGVCRTVRVASGPVHGAQVIQGRSDG

Sequences:

>Translated_314_residues
MSETVSDWVPTGAVTVRAPGKVNLYLAVGDLRDDGYHELTTVFHAVSLADDVTVRDADVLSIDVVGQGEGTVPTDERNLA
WQAAELFADHVGRAPDVSIFINKDIPVAGGMAGGSADAAAVLVAMNELWHAGVPRRDLHHLAAQLGSDVPFALHGGTALG
TGRGEQLATVLARNVFHWVFAFADGGLATPQVFKEIDRLRENGDPPRLAEADELLGALAAGDARRLAPLLGNELQAAAVS
LNPELRRTLRAGESAGALAGIVSGSGPTCAFLCTSADDAVQVSAELAGAGVCRTVRVASGPVHGAQVIQGRSDG
>Mature_313_residues
SETVSDWVPTGAVTVRAPGKVNLYLAVGDLRDDGYHELTTVFHAVSLADDVTVRDADVLSIDVVGQGEGTVPTDERNLAW
QAAELFADHVGRAPDVSIFINKDIPVAGGMAGGSADAAAVLVAMNELWHAGVPRRDLHHLAAQLGSDVPFALHGGTALGT
GRGEQLATVLARNVFHWVFAFADGGLATPQVFKEIDRLRENGDPPRLAEADELLGALAAGDARRLAPLLGNELQAAAVSL
NPELRRTLRAGESAGALAGIVSGSGPTCAFLCTSADDAVQVSAELAGAGVCRTVRVASGPVHGAQVIQGRSDG

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=184, Percent_Identity=35.8695652173913, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786184, Length=255, Percent_Identity=26.2745098039216, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 32413; Mature: 32281

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVSDWVPTGAVTVRAPGKVNLYLAVGDLRDDGYHELTTVFHAVSLADDVTVRDADVL
CCCCHHHHCCCCEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCEE
SIDVVGQGEGTVPTDERNLAWQAAELFADHVGRAPDVSIFINKDIPVAGGMAGGSADAAA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
VLVAMNELWHAGVPRRDLHHLAAQLGSDVPFALHGGTALGTGRGEQLATVLARNVFHWVF
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHH
AFADGGLATPQVFKEIDRLRENGDPPRLAEADELLGALAAGDARRLAPLLGNELQAAAVS
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHEEEE
LNPELRRTLRAGESAGALAGIVSGSGPTCAFLCTSADDAVQVSAELAGAGVCRTVRVASG
CCHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEHHHHCCCCCEEEEEECCC
PVHGAQVIQGRSDG
CCCCHHHCCCCCCC
>Mature Secondary Structure 
SETVSDWVPTGAVTVRAPGKVNLYLAVGDLRDDGYHELTTVFHAVSLADDVTVRDADVL
CCCHHHHCCCCEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCEE
SIDVVGQGEGTVPTDERNLAWQAAELFADHVGRAPDVSIFINKDIPVAGGMAGGSADAAA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
VLVAMNELWHAGVPRRDLHHLAAQLGSDVPFALHGGTALGTGRGEQLATVLARNVFHWVF
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHH
AFADGGLATPQVFKEIDRLRENGDPPRLAEADELLGALAAGDARRLAPLLGNELQAAAVS
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHEEEE
LNPELRRTLRAGESAGALAGIVSGSGPTCAFLCTSADDAVQVSAELAGAGVCRTVRVASG
CCHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEHHHHCCCCCEEEEEECCC
PVHGAQVIQGRSDG
CCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA