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The map label for this gene is glpF [H]

Identifier: 169627485

GI number: 169627485

Start: 377163

End: 377966

Strand: Direct

Name: glpF [H]

Synonym: MAB_0381

Alternate gene names: 169627485

Gene position: 377163-377966 (Clockwise)

Preceding gene: 169627484

Following gene: 169627486

Centisome position: 7.44

GC content: 63.43

Gene sequence:

>804_bases
ATGGGTCAACTCGCCGCGGAGTTCGTCGGCACCCTGATCTTGATCCTGTTCGGGGTGGGCGTCGTCGCGCAGGTGGTCAC
GGCGGGCGACGAGGATTACGGCAACCACAACTCCATCGCCTGGGCCTGGGGCATGGGAGTCACCTTCGGCATCTTCGCGG
CCGGGCGGATATCCGGCGCGCATCTCAACCCGGCGGTCACGGTGGCCCTGGCCGTCTTTCGCGGATTCCCATGGAAACAG
GTAATTCCATTCATCCTCGCTCAGGTCGGCGGCGCATTTGTCGCGGCGCTGCTGGTCCGGTGGAACTATGGAAGTGTGCT
GGCGGCGATCGATCCCGGGCACACCATCGCGACGCAGGGCGTGTTCTCGACATTGCCGGGTAACGGCGCGCTCGACGTCA
GCCTGTGGGGCGGCCTGCGTGACCAGATCATGGGCACCGCCATTCTGCTGTTTCTGATCTTCGCCATCACGGACCGGCGG
AATATTCGCATGCCCGAGATATTCGCCGCGCTTTCGATCGGTGCTGTGGTCGTCGGATTGGGTATGGCATGGGGGTCCAT
CGCCGGGTACGCGATCAACCCGGCCCGTGACTTCGGTCCACGGCTCGCGTCCTATCTCACCGGTTTCGATACGGCCTTTC
TGGACCAGCACGGTGCCCAGTACTGGTGGGTGCCGATCGTCGGGCCGTTCATCGGTGGTCTGGTGGGCGCCGCGCTATAC
CGGTACCTGGTCGAACCATTCCTACCCGCAGATGATCCTGTAACCCAATCACCTTCGCATACCGAGGAGTTCGCCAATGG
CTGA

Upstream 100 bases:

>100_bases
AGCCAGTCGGTAGCGAATAGCCCTCCGCGCGCGGAGGGCTATTCGGCGTTTCGGGGCGTGCCCAGCGCAGCTCGCACCTA
GATTCGGTGATATGCGGAAC

Downstream 100 bases:

>100_bases
TTTCGTGGCATCGATCGACCAAGGCACTACCAGCACACGCGCCATGATCTTCAACCACAGGGGCGAGGAAGTGGGCCGCC
ATCAGCTGGAGCATCAGCAA

Product: glycerol uptake facilitator protein (GlpF)

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MGQLAAEFVGTLILILFGVGVVAQVVTAGDEDYGNHNSIAWAWGMGVTFGIFAAGRISGAHLNPAVTVALAVFRGFPWKQ
VIPFILAQVGGAFVAALLVRWNYGSVLAAIDPGHTIATQGVFSTLPGNGALDVSLWGGLRDQIMGTAILLFLIFAITDRR
NIRMPEIFAALSIGAVVVGLGMAWGSIAGYAINPARDFGPRLASYLTGFDTAFLDQHGAQYWWVPIVGPFIGGLVGAALY
RYLVEPFLPADDPVTQSPSHTEEFANG

Sequences:

>Translated_267_residues
MGQLAAEFVGTLILILFGVGVVAQVVTAGDEDYGNHNSIAWAWGMGVTFGIFAAGRISGAHLNPAVTVALAVFRGFPWKQ
VIPFILAQVGGAFVAALLVRWNYGSVLAAIDPGHTIATQGVFSTLPGNGALDVSLWGGLRDQIMGTAILLFLIFAITDRR
NIRMPEIFAALSIGAVVVGLGMAWGSIAGYAINPARDFGPRLASYLTGFDTAFLDQHGAQYWWVPIVGPFIGGLVGAALY
RYLVEPFLPADDPVTQSPSHTEEFANG
>Mature_266_residues
GQLAAEFVGTLILILFGVGVVAQVVTAGDEDYGNHNSIAWAWGMGVTFGIFAAGRISGAHLNPAVTVALAVFRGFPWKQV
IPFILAQVGGAFVAALLVRWNYGSVLAAIDPGHTIATQGVFSTLPGNGALDVSLWGGLRDQIMGTAILLFLIFAITDRRN
IRMPEIFAALSIGAVVVGLGMAWGSIAGYAINPARDFGPRLASYLTGFDTAFLDQHGAQYWWVPIVGPFIGGLVGAALYR
YLVEPFLPADDPVTQSPSHTEEFANG

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=271, Percent_Identity=40.2214022140221, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI22538420, Length=270, Percent_Identity=36.2962962962963, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI157266307, Length=267, Percent_Identity=34.8314606741573, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI4502187, Length=255, Percent_Identity=34.1176470588235, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI310133356, Length=216, Percent_Identity=32.4074074074074, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI310114181, Length=216, Percent_Identity=32.4074074074074, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4502179, Length=242, Percent_Identity=31.8181818181818, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37694062, Length=255, Percent_Identity=27.0588235294118, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4755125, Length=246, Percent_Identity=29.2682926829268, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4502181, Length=246, Percent_Identity=29.2682926829268, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI6912506, Length=256, Percent_Identity=30.46875, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1790362, Length=269, Percent_Identity=39.7769516728625, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1787101, Length=197, Percent_Identity=32.48730964467, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=38.0566801619433, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32564052, Length=270, Percent_Identity=33.7037037037037, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=270, Percent_Identity=33.7037037037037, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71994009, Length=255, Percent_Identity=32.156862745098, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531429, Length=251, Percent_Identity=35.8565737051793, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17531431, Length=251, Percent_Identity=35.8565737051793, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71992961, Length=247, Percent_Identity=33.1983805668016, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17544068, Length=268, Percent_Identity=32.4626865671642, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17558372, Length=245, Percent_Identity=28.9795918367347, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=210, Percent_Identity=37.6190476190476, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6322985, Length=286, Percent_Identity=33.2167832167832, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550503, Length=266, Percent_Identity=25.9398496240602, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24762344, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24762346, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24762348, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130305, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24762342, Length=216, Percent_Identity=26.8518518518519, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28222; Mature: 28091

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQLAAEFVGTLILILFGVGVVAQVVTAGDEDYGNHNSIAWAWGMGVTFGIFAAGRISGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCHHHHHHHHHCCCCCC
HLNPAVTVALAVFRGFPWKQVIPFILAQVGGAFVAALLVRWNYGSVLAAIDPGHTIATQG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCC
VFSTLPGNGALDVSLWGGLRDQIMGTAILLFLIFAITDRRNIRMPEIFAALSIGAVVVGL
HHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
GMAWGSIAGYAINPARDFGPRLASYLTGFDTAFLDQHGAQYWWVPIVGPFIGGLVGAALY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
RYLVEPFLPADDPVTQSPSHTEEFANG
HHHHHHCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
GQLAAEFVGTLILILFGVGVVAQVVTAGDEDYGNHNSIAWAWGMGVTFGIFAAGRISGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCHHHHHHHHHCCCCCC
HLNPAVTVALAVFRGFPWKQVIPFILAQVGGAFVAALLVRWNYGSVLAAIDPGHTIATQG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCC
VFSTLPGNGALDVSLWGGLRDQIMGTAILLFLIFAITDRRNIRMPEIFAALSIGAVVVGL
HHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
GMAWGSIAGYAINPARDFGPRLASYLTGFDTAFLDQHGAQYWWVPIVGPFIGGLVGAALY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
RYLVEPFLPADDPVTQSPSHTEEFANG
HHHHHHCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]