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The map label for this gene is glpK

Identifier: 169627486

GI number: 169627486

Start: 377959

End: 379473

Strand: Direct

Name: glpK

Synonym: MAB_0382

Alternate gene names: 169627486

Gene position: 377959-379473 (Clockwise)

Preceding gene: 169627485

Following gene: 169627488

Centisome position: 7.46

GC content: 63.89

Gene sequence:

>1515_bases
ATGGCTGATTTCGTGGCATCGATCGACCAAGGCACTACCAGCACACGCGCCATGATCTTCAACCACAGGGGCGAGGAAGT
GGGCCGCCATCAGCTGGAGCATCAGCAACTGCTGCCACGGGCGGGCTGGGTGGAACACAACCCGGTGGAGATCTGGGAGC
GCACCTGGTCGGTGTTGGCCACGTCTCTCAATGTGACCGGGCTATCGGCCGGTGATCTCGCTGCCGTCGGGGTGACCAAT
CAGCGTGAGACCACCTTGGTGTGGAACCGCCACACTGGTAGGCCGTACTGCAATGCCATCGTGTGGCAGGACACCCGCAC
CGACAAGATCGCCGCGGCACTGGACCGCGACGGGCGTGGTGACATCATTCGGCGGAAGGCCGGACTACCTCCCGCCACTT
ACTTTTCGGGCGGGAAGCTGCAATGGATTCTGGAGAACGTCGAGGGGGTGCGGCGGGATGCGGAGAATGGTGACGCGCTC
TTCGGGACGCCCGACAGCTGGGTGATCTGGAATCTCACCGGCGGTGTCCGCAGCGGGGTACATGTCACCGATGTGACAAA
TGCCAGTCGCACCATGTTGATGAACCTGGAGACCCTGGACTGGGATGACGAGCTGTTGTCGTTCTTTTCCATTCCGCGCC
AGATGCTTCCGCCGATCAAGGCGTCGTCGCCGGTGGAGCCGTTCGGCTTCACGACGCAGCTGGGTCCGCTGGGCGGAGAG
GTTCCCATCGCCGGCGATCTGGGTGATCAGCAGGCCGCGATGGTGGGGCAGGTGTGCCTGAACCCCGGCGAGGCCAAGAA
CACCTACGGCACCGGAAACTTCTTGCTACTCAACACCGGTGAGGAACTGGTCCATTCGAGCAATGGGCTGTTGACCACCG
TCTGTTATCAATTCGGCGACAACAAGCCGGTGTATGCGCTGGAGGGATCGATCGCGGTGACGGGCTCCGCGGTGCAGTGG
CTGCGCGACCAGCTGGGCATCATCAGCGGTGCCTCGCAAAGCGAGGACCTGGCGCGCCAGGTCGAGGACAACGGCGGCGT
CTATTTCGTGCCGGCCTTTTCCGGGCTGTTCGCGCCGTACTGGCGATCGGATGCACGCGGCGCCATCGTGGGTCTGTCCC
GGTTCAACACCAACGCGCACCTGGCGCGCGCGACACTCGAAGCCATCTGTTACCAGAGCCGGGAAGTGGTCGAGGCCATG
GAGGCGGACTCGGGTGTGCGGATGGAGGTGCTCAAGGTCGATGGCGGTATCACCGCCAACAAGCTCTGCATGCAGATCCA
AGCCGACACCCTGGGTGTGGACGTCGTCAAACCCGTTGTCGCCGAGACTACTGCGCTGGGCGCGGCGTACGCGGCGGGCT
TGGCCGTGGGTTTCTGGAAGGACGCCGACGACCTGCGGCGCAACTGGCAGGAAGACGAACGCTGGAGCTCGTCGATCACG
GACGAGAAGCGCGCCGAGGGTTTCGCGGGCTGGAAGAAAGCCGTGCAGCGCACGCTGGACGGGGTGGACGTCTAA

Upstream 100 bases:

>100_bases
GGTCTGGTGGGCGCCGCGCTATACCGGTACCTGGTCGAACCATTCCTACCCGCAGATGATCCTGTAACCCAATCACCTTC
GCATACCGAGGAGTTCGCCA

Downstream 100 bases:

>100_bases
AGCAGTGTGCCGAGTGTCGGCTTTTCGGACAGTTCTCGAACTGTCGCCCGAAAAGCCGACACTCGACGGCGTTGAGCTAG
TCGGTTTCACCCAAGATGCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MADFVASIDQGTTSTRAMIFNHRGEEVGRHQLEHQQLLPRAGWVEHNPVEIWERTWSVLATSLNVTGLSAGDLAAVGVTN
QRETTLVWNRHTGRPYCNAIVWQDTRTDKIAAALDRDGRGDIIRRKAGLPPATYFSGGKLQWILENVEGVRRDAENGDAL
FGTPDSWVIWNLTGGVRSGVHVTDVTNASRTMLMNLETLDWDDELLSFFSIPRQMLPPIKASSPVEPFGFTTQLGPLGGE
VPIAGDLGDQQAAMVGQVCLNPGEAKNTYGTGNFLLLNTGEELVHSSNGLLTTVCYQFGDNKPVYALEGSIAVTGSAVQW
LRDQLGIISGASQSEDLARQVEDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSREVVEAM
EADSGVRMEVLKVDGGITANKLCMQIQADTLGVDVVKPVVAETTALGAAYAAGLAVGFWKDADDLRRNWQEDERWSSSIT
DEKRAEGFAGWKKAVQRTLDGVDV

Sequences:

>Translated_504_residues
MADFVASIDQGTTSTRAMIFNHRGEEVGRHQLEHQQLLPRAGWVEHNPVEIWERTWSVLATSLNVTGLSAGDLAAVGVTN
QRETTLVWNRHTGRPYCNAIVWQDTRTDKIAAALDRDGRGDIIRRKAGLPPATYFSGGKLQWILENVEGVRRDAENGDAL
FGTPDSWVIWNLTGGVRSGVHVTDVTNASRTMLMNLETLDWDDELLSFFSIPRQMLPPIKASSPVEPFGFTTQLGPLGGE
VPIAGDLGDQQAAMVGQVCLNPGEAKNTYGTGNFLLLNTGEELVHSSNGLLTTVCYQFGDNKPVYALEGSIAVTGSAVQW
LRDQLGIISGASQSEDLARQVEDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSREVVEAM
EADSGVRMEVLKVDGGITANKLCMQIQADTLGVDVVKPVVAETTALGAAYAAGLAVGFWKDADDLRRNWQEDERWSSSIT
DEKRAEGFAGWKKAVQRTLDGVDV
>Mature_503_residues
ADFVASIDQGTTSTRAMIFNHRGEEVGRHQLEHQQLLPRAGWVEHNPVEIWERTWSVLATSLNVTGLSAGDLAAVGVTNQ
RETTLVWNRHTGRPYCNAIVWQDTRTDKIAAALDRDGRGDIIRRKAGLPPATYFSGGKLQWILENVEGVRRDAENGDALF
GTPDSWVIWNLTGGVRSGVHVTDVTNASRTMLMNLETLDWDDELLSFFSIPRQMLPPIKASSPVEPFGFTTQLGPLGGEV
PIAGDLGDQQAAMVGQVCLNPGEAKNTYGTGNFLLLNTGEELVHSSNGLLTTVCYQFGDNKPVYALEGSIAVTGSAVQWL
RDQLGIISGASQSEDLARQVEDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSREVVEAME
ADSGVRMEVLKVDGGITANKLCMQIQADTLGVDVVKPVVAETTALGAAYAAGLAVGFWKDADDLRRNWQEDERWSSSITD
EKRAEGFAGWKKAVQRTLDGVDV

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=509, Percent_Identity=48.1335952848723, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI42794763, Length=512, Percent_Identity=47.4609375, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI4504007, Length=509, Percent_Identity=48.1335952848723, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI41393575, Length=505, Percent_Identity=47.5247524752475, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=30.6042884990253, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=51.307847082495, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1790005, Length=503, Percent_Identity=24.2544731610338, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787789, Length=533, Percent_Identity=24.015009380863, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789987, Length=509, Percent_Identity=22.0039292730845, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=45.9405940594059, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=24.3761996161228, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=27.8523489932886, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=550, Percent_Identity=39.6363636363636, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=46.3654223968566, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=48.7414187643021, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655555, Length=509, Percent_Identity=41.6502946954813, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655560, Length=509, Percent_Identity=41.6502946954813, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655570, Length=509, Percent_Identity=41.6502946954813, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655574, Length=509, Percent_Identity=41.6502946954813, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655565, Length=509, Percent_Identity=41.6502946954813, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=34.5351043643264, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=34.6855983772819, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24656367, Length=490, Percent_Identity=29.5918367346939, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21357867, Length=503, Percent_Identity=29.2246520874751, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24656372, Length=319, Percent_Identity=33.8557993730408, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI45552909, Length=295, Percent_Identity=30.5084745762712, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_MYCA9 (B1MFT1)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701135.1
- ProteinModelPortal:   B1MFT1
- SMR:   B1MFT1
- EnsemblBacteria:   EBMYCT00000008939
- GeneID:   5962919
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_0382
- GeneTree:   EBGT00050000017511
- HOGENOM:   HBG511469
- OMA:   DSGVRLE
- ProtClustDB:   PRK00047
- BioCyc:   MABS561007:MAB_0382-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54843; Mature: 54712

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 249-249 BINDING 271-271 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFVASIDQGTTSTRAMIFNHRGEEVGRHQLEHQQLLPRAGWVEHNPVEIWERTWSVLA
CCCHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TSLNVTGLSAGDLAAVGVTNQRETTLVWNRHTGRPYCNAIVWQDTRTDKIAAALDRDGRG
HHCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHCEEEECCCCCHHHHHHHCCCCCC
DIIRRKAGLPPATYFSGGKLQWILENVEGVRRDAENGDALFGTPDSWVIWNLTGGVRSGV
CEEHHHCCCCCCCEECCCEEEHHHHHHHHHHHCCCCCCEEECCCCCEEEEEECCCCCCCE
HVTDVTNASRTMLMNLETLDWDDELLSFFSIPRQMLPPIKASSPVEPFGFTTQLGPLGGE
EEEECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCCC
VPIAGDLGDQQAAMVGQVCLNPGEAKNTYGTGNFLLLNTGEELVHSSNGLLTTVCYQFGD
CCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEHHHHHCC
NKPVYALEGSIAVTGSAVQWLRDQLGIISGASQSEDLARQVEDNGGVYFVPAFSGLFAPY
CCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHCHH
WRSDARGAIVGLSRFNTNAHLARATLEAICYQSREVVEAMEADSGVRMEVLKVDGGITAN
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
KLCMQIQADTLGVDVVKPVVAETTALGAAYAAGLAVGFWKDADDLRRNWQEDERWSSSIT
HEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCHHHHHHHCCC
DEKRAEGFAGWKKAVQRTLDGVDV
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADFVASIDQGTTSTRAMIFNHRGEEVGRHQLEHQQLLPRAGWVEHNPVEIWERTWSVLA
CCHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TSLNVTGLSAGDLAAVGVTNQRETTLVWNRHTGRPYCNAIVWQDTRTDKIAAALDRDGRG
HHCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHCEEEECCCCCHHHHHHHCCCCCC
DIIRRKAGLPPATYFSGGKLQWILENVEGVRRDAENGDALFGTPDSWVIWNLTGGVRSGV
CEEHHHCCCCCCCEECCCEEEHHHHHHHHHHHCCCCCCEEECCCCCEEEEEECCCCCCCE
HVTDVTNASRTMLMNLETLDWDDELLSFFSIPRQMLPPIKASSPVEPFGFTTQLGPLGGE
EEEECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCCC
VPIAGDLGDQQAAMVGQVCLNPGEAKNTYGTGNFLLLNTGEELVHSSNGLLTTVCYQFGD
CCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEHHHHHCC
NKPVYALEGSIAVTGSAVQWLRDQLGIISGASQSEDLARQVEDNGGVYFVPAFSGLFAPY
CCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHCHH
WRSDARGAIVGLSRFNTNAHLARATLEAICYQSREVVEAMEADSGVRMEVLKVDGGITAN
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
KLCMQIQADTLGVDVVKPVVAETTALGAAYAAGLAVGFWKDADDLRRNWQEDERWSSSIT
HEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCHHHHHHHCCC
DEKRAEGFAGWKKAVQRTLDGVDV
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA