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The map label for this gene is amt [H]

Identifier: 169627459

GI number: 169627459

Start: 352611

End: 353936

Strand: Direct

Name: amt [H]

Synonym: MAB_0355

Alternate gene names: 169627459

Gene position: 352611-353936 (Clockwise)

Preceding gene: 169627457

Following gene: 169627462

Centisome position: 6.96

GC content: 64.93

Gene sequence:

>1326_bases
ATGTTTCCGCATATGGGAGTGCCGGACGCGGCCAATACGGCCTGGGTGTTGACGAGTGCCGCGCTGGTGCTCCTGATGAC
CCCGGCACTGGCCTTCTTCTACGGCGGCATGGTGCGCGCCAAGAGCGTGCTCAACATGATGATGATGTGCTTTTCGGCCG
TCGCCGTGGTGTGGCTGCTCTGGGTGATCTTCGGATATTCGATGGCGTTCGGAACCGATATCGGCGGTGGACTGCTCGGT
GATCCGTTCCAGTTCGCCGGGCTGCAACATTTGTTCGAGGGCGACTACAAGGCACCCTCGGTTCCGTTGTGGGGGCCGAC
GAATATCCCGGCGCTGGTGTTCGTCATGTTCCAGGCCGGGTTCGCGATGGTGACGGTAGCGCTCATCGCGGGCGCAGTGG
CCGACCGTATGCGCTTCCTGCCGTGGATAGCCTTCACCGCGTTGTGGGCCACGCTGGTGTATTTCCCGGTCGCGCACTGG
ATATTCGCGGTCGAAGGCACCACTGCGGACAAGGGCGGATGGCTTGCCAACCTCGGTGTGCTCGATTTCGCCGGTGGCAC
CGCGGTGGAGATCAACTCGGGTGCTGCGGGCTTGGCGCTGGCCATTGTCCTGGGTAAGCGGCGCGGGTGGCCGCGTGAGG
CGATGCGCCCGCATAACCTGCCCGCCGTCATGCTGGGAGCCGGGCTGTTGTGGTTCGGCTGGTTCGGCTTCAACGCCGGA
TCGGCGTTGGCGGCCGACGGCACCGCAGCGCTTGTCATTGCCAACACCCTTGGGGCGGGGGCGATATCGATGGCCAGCTG
GCTGTTGGTGGAGAAGGTCCGGCATGGCAAGCCGACCTCGTTTGGCGCCGCATCGGGTGTGGTCGCGGGCCTGGTGGCCA
TCACTCCCTCGTGCGCGGCGGTCACCCCGATCGGTGCGCTGATTATCGGTGCCGTGACCGGTGCCGTCAGCTCACTTGCC
ATCGAGCTGAAATACCGTTTGGGATATGACGATTCGCTCGACGTAGTGGGCGTGCACCTGGTGGGTGGCGTGGTCGGCAC
GTTGATGATCGGCGTCGTCGGCAGCGCCGCGGCGCCTGCTGGTGTCAACGGGCTGCTGTACGGTGGCGGGCTGCATCAGT
TCTGGTTGCAGCTTGTCGGCGTCGTCACGGTGCTTGTCTACTCGGTCGCGGTCACCGGACTCATCGGCCTGGGCTTACGT
CATTCCCTGGGTATCCGGTCGCATCCGCAGCACGAGTCGGATGGGCTCGATGACGCCGAGCACGCGGAGTCGGCGTATGA
GCTGGCCACCACGCGCGGCGGCGGTCTTATCAACCCTGGACGTTGA

Upstream 100 bases:

>100_bases
ACTGGAACGTGTTCTAGTTTAGATCAGGTCCTGCGCACGCGGGCGGCGGCTTCCCTACACTCACGCTGACCTAATCGCAC
GAGCCGGGAGTAGCCAGCGC

Downstream 100 bases:

>100_bases
ACCTGCCGATCCCGGGTATGAAACGTCCAACTCGCGCTCCGTATTCGCGATAGGTGGTGCCGTGCGCCGCTAATAGATAG
GGCTCCTCGACGTCGCGTAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MFPHMGVPDAANTAWVLTSAALVLLMTPALAFFYGGMVRAKSVLNMMMMCFSAVAVVWLLWVIFGYSMAFGTDIGGGLLG
DPFQFAGLQHLFEGDYKAPSVPLWGPTNIPALVFVMFQAGFAMVTVALIAGAVADRMRFLPWIAFTALWATLVYFPVAHW
IFAVEGTTADKGGWLANLGVLDFAGGTAVEINSGAAGLALAIVLGKRRGWPREAMRPHNLPAVMLGAGLLWFGWFGFNAG
SALAADGTAALVIANTLGAGAISMASWLLVEKVRHGKPTSFGAASGVVAGLVAITPSCAAVTPIGALIIGAVTGAVSSLA
IELKYRLGYDDSLDVVGVHLVGGVVGTLMIGVVGSAAAPAGVNGLLYGGGLHQFWLQLVGVVTVLVYSVAVTGLIGLGLR
HSLGIRSHPQHESDGLDDAEHAESAYELATTRGGGLINPGR

Sequences:

>Translated_441_residues
MFPHMGVPDAANTAWVLTSAALVLLMTPALAFFYGGMVRAKSVLNMMMMCFSAVAVVWLLWVIFGYSMAFGTDIGGGLLG
DPFQFAGLQHLFEGDYKAPSVPLWGPTNIPALVFVMFQAGFAMVTVALIAGAVADRMRFLPWIAFTALWATLVYFPVAHW
IFAVEGTTADKGGWLANLGVLDFAGGTAVEINSGAAGLALAIVLGKRRGWPREAMRPHNLPAVMLGAGLLWFGWFGFNAG
SALAADGTAALVIANTLGAGAISMASWLLVEKVRHGKPTSFGAASGVVAGLVAITPSCAAVTPIGALIIGAVTGAVSSLA
IELKYRLGYDDSLDVVGVHLVGGVVGTLMIGVVGSAAAPAGVNGLLYGGGLHQFWLQLVGVVTVLVYSVAVTGLIGLGLR
HSLGIRSHPQHESDGLDDAEHAESAYELATTRGGGLINPGR
>Mature_441_residues
MFPHMGVPDAANTAWVLTSAALVLLMTPALAFFYGGMVRAKSVLNMMMMCFSAVAVVWLLWVIFGYSMAFGTDIGGGLLG
DPFQFAGLQHLFEGDYKAPSVPLWGPTNIPALVFVMFQAGFAMVTVALIAGAVADRMRFLPWIAFTALWATLVYFPVAHW
IFAVEGTTADKGGWLANLGVLDFAGGTAVEINSGAAGLALAIVLGKRRGWPREAMRPHNLPAVMLGAGLLWFGWFGFNAG
SALAADGTAALVIANTLGAGAISMASWLLVEKVRHGKPTSFGAASGVVAGLVAITPSCAAVTPIGALIIGAVTGAVSSLA
IELKYRLGYDDSLDVVGVHLVGGVVGTLMIGVVGSAAAPAGVNGLLYGGGLHQFWLQLVGVVTVLVYSVAVTGLIGLGLR
HSLGIRSHPQHESDGLDDAEHAESAYELATTRGGGLINPGR

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=425, Percent_Identity=41.6470588235294, Blast_Score=272, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI32564042, Length=434, Percent_Identity=27.4193548387097, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=451, Percent_Identity=27.4944567627494, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17550180, Length=452, Percent_Identity=25.8849557522124, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=439, Percent_Identity=25.5125284738041, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17563228, Length=203, Percent_Identity=30.5418719211823, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324187, Length=440, Percent_Identity=35, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6325396, Length=435, Percent_Identity=34.2528735632184, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=433, Percent_Identity=32.7944572748268, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI161078314, Length=442, Percent_Identity=28.7330316742081, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45618; Mature: 45618

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPHMGVPDAANTAWVLTSAALVLLMTPALAFFYGGMVRAKSVLNMMMMCFSAVAVVWLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVIFGYSMAFGTDIGGGLLGDPFQFAGLQHLFEGDYKAPSVPLWGPTNIPALVFVMFQAG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FAMVTVALIAGAVADRMRFLPWIAFTALWATLVYFPVAHWIFAVEGTTADKGGWLANLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCE
LDFAGGTAVEINSGAAGLALAIVLGKRRGWPREAMRPHNLPAVMLGAGLLWFGWFGFNAG
EEECCCCEEEECCCCHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
SALAADGTAALVIANTLGAGAISMASWLLVEKVRHGKPTSFGAASGVVAGLVAITPSCAA
CEEECCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHH
VTPIGALIIGAVTGAVSSLAIELKYRLGYDDSLDVVGVHLVGGVVGTLMIGVVGSAAAPA
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVNGLLYGGGLHQFWLQLVGVVTVLVYSVAVTGLIGLGLRHSLGIRSHPQHESDGLDDAE
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
HAESAYELATTRGGGLINPGR
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFPHMGVPDAANTAWVLTSAALVLLMTPALAFFYGGMVRAKSVLNMMMMCFSAVAVVWLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVIFGYSMAFGTDIGGGLLGDPFQFAGLQHLFEGDYKAPSVPLWGPTNIPALVFVMFQAG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FAMVTVALIAGAVADRMRFLPWIAFTALWATLVYFPVAHWIFAVEGTTADKGGWLANLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCE
LDFAGGTAVEINSGAAGLALAIVLGKRRGWPREAMRPHNLPAVMLGAGLLWFGWFGFNAG
EEECCCCEEEECCCCHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
SALAADGTAALVIANTLGAGAISMASWLLVEKVRHGKPTSFGAASGVVAGLVAITPSCAA
CEEECCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHH
VTPIGALIIGAVTGAVSSLAIELKYRLGYDDSLDVVGVHLVGGVVGTLMIGVVGSAAAPA
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVNGLLYGGGLHQFWLQLVGVVTVLVYSVAVTGLIGLGLRHSLGIRSHPQHESDGLDDAE
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
HAESAYELATTRGGGLINPGR
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]