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The map label for this gene is caiD [H]

Identifier: 169627209

GI number: 169627209

Start: 105363

End: 106145

Strand: Direct

Name: caiD [H]

Synonym: MAB_0104

Alternate gene names: 169627209

Gene position: 105363-106145 (Clockwise)

Preceding gene: 169627208

Following gene: 169627217

Centisome position: 2.08

GC content: 67.43

Gene sequence:

>783_bases
GTGACTGAACTCCACGAAGAAATTCGTGACGGGGTAGCGGTTCTCACGCTGCACGGACCGTCGACGCGGAACTCCTTCAC
GGTCGAGCTGGGTCGTCAACTCGGCGCCGCATATCAGCGGCTCGACGACGATCCAGCTGTTCGTGTCATCGTGCTCACCG
GCGCGCCACCCGCGTTCTGCAGTGGTGCCCAGATCTCGGCGGCCGCGGAAACATTTGCGGCGCCGCGTAATCCGGACTTC
TCGGCGTCGCCGGTGCAACCGGCCGCGTTCGAGCTGAGAACCCCCGTCATCGCGGCGGTGAACGGACACGCGATCGGTAT
AGGAATGACGCTGGCGTTGCATGCGGACATCCGCATCCTTGCCGAGGAGGGGCGTTATGCCATACCGCAGGTGCGGTTCG
GAGTGGCACCCGATGCGCTGGCGCACTGGACGCTGCCCAGGCTGGTCGGTACTGCGGTAGCGGCGGAGCTACTGCTCACC
GGCGCGAGTTTCTCGGCACAGCGGGCCGTCGAGACAGGCTTGGCCAACCGGTGTCTGCCCGCAGGAAAGGTACTGGGTGC
GGCCCTGAGGATGGCTCACGATATCGCGACAAACGTGGCGCCCGAGTCCGCGGCCCTGACCAAGCGGCTGCTCTGGGATG
CGCAGATGACGGGGATGTCAGCGGCCGAGGTGGCGGCACGGGAGACGGCAGACCACCTGCGTTTGATGGGCTCGCAGGAT
GCGGCCGAGGGCCCGCGCGCGTTCATCGACGGCCGCCCGCCGCGCTGGGCCGGTCAGCGGTAG

Upstream 100 bases:

>100_bases
CGGGATGATGCGGCGCAGCCGGGTCACCGAGGGTATGCGGTTCGGGCGGAAGAAGACGGCGGCGGCGCCGGCATCTGAAT
CGGTGGCCGTCAACAGCTAG

Downstream 100 bases:

>100_bases
CTCCGCCGTCACCCACGGTTGAATCATCCCGGCGGGGCGCCTATTCTGGGTATCAACCGTATCCAGATGGCGAAGGAGCT
CTGCGTGGGAAGACACCACC

Product: enoyl-CoA hydratase/isomerase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTELHEEIRDGVAVLTLHGPSTRNSFTVELGRQLGAAYQRLDDDPAVRVIVLTGAPPAFCSGAQISAAAETFAAPRNPDF
SASPVQPAAFELRTPVIAAVNGHAIGIGMTLALHADIRILAEEGRYAIPQVRFGVAPDALAHWTLPRLVGTAVAAELLLT
GASFSAQRAVETGLANRCLPAGKVLGAALRMAHDIATNVAPESAALTKRLLWDAQMTGMSAAEVAARETADHLRLMGSQD
AAEGPRAFIDGRPPRWAGQR

Sequences:

>Translated_260_residues
MTELHEEIRDGVAVLTLHGPSTRNSFTVELGRQLGAAYQRLDDDPAVRVIVLTGAPPAFCSGAQISAAAETFAAPRNPDF
SASPVQPAAFELRTPVIAAVNGHAIGIGMTLALHADIRILAEEGRYAIPQVRFGVAPDALAHWTLPRLVGTAVAAELLLT
GASFSAQRAVETGLANRCLPAGKVLGAALRMAHDIATNVAPESAALTKRLLWDAQMTGMSAAEVAARETADHLRLMGSQD
AAEGPRAFIDGRPPRWAGQR
>Mature_259_residues
TELHEEIRDGVAVLTLHGPSTRNSFTVELGRQLGAAYQRLDDDPAVRVIVLTGAPPAFCSGAQISAAAETFAAPRNPDFS
ASPVQPAAFELRTPVIAAVNGHAIGIGMTLALHADIRILAEEGRYAIPQVRFGVAPDALAHWTLPRLVGTAVAAELLLTG
ASFSAQRAVETGLANRCLPAGKVLGAALRMAHDIATNVAPESAALTKRLLWDAQMTGMSAAEVAARETADHLRLMGSQDA
AEGPRAFIDGRPPRWAGQR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=30.1158301158301, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=30.3846153846154, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=27.6923076923077, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=183, Percent_Identity=27.3224043715847, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594469, Length=183, Percent_Identity=27.3224043715847, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI70995211, Length=189, Percent_Identity=26.4550264550265, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI116235487, Length=162, Percent_Identity=24.6913580246914, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=31.3868613138686, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=31.9230769230769, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=31.9548872180451, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=26.3358778625954, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=27.027027027027, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082183, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=33.0645161290323, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=31.1023622047244, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=204, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=206, Percent_Identity=28.1553398058252, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=26.9406392694064, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=194, Percent_Identity=27.8350515463918, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=212, Percent_Identity=25.4716981132075, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=205, Percent_Identity=27.8048780487805, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=206, Percent_Identity=28.1553398058252, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=206, Percent_Identity=28.1553398058252, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=259, Percent_Identity=23.9382239382239, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=206, Percent_Identity=28.1553398058252, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=24.5901639344262, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=31.0606060606061, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=31.0606060606061, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=27.9245283018868, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357171, Length=193, Percent_Identity=30.5699481865285, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=26.953125, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920382, Length=234, Percent_Identity=28.6324786324786, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650670, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27333; Mature: 27202

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELHEEIRDGVAVLTLHGPSTRNSFTVELGRQLGAAYQRLDDDPAVRVIVLTGAPPAFC
CCHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
SGAQISAAAETFAAPRNPDFSASPVQPAAFELRTPVIAAVNGHAIGIGMTLALHADIRIL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEEEEEEEEEEECCEEEE
AEEGRYAIPQVRFGVAPDALAHWTLPRLVGTAVAAELLLTGASFSAQRAVETGLANRCLP
ECCCCEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
AGKVLGAALRMAHDIATNVAPESAALTKRLLWDAQMTGMSAAEVAARETADHLRLMGSQD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
AAEGPRAFIDGRPPRWAGQR
CCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
TELHEEIRDGVAVLTLHGPSTRNSFTVELGRQLGAAYQRLDDDPAVRVIVLTGAPPAFC
CHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
SGAQISAAAETFAAPRNPDFSASPVQPAAFELRTPVIAAVNGHAIGIGMTLALHADIRIL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEEEEEEEEEEECCEEEE
AEEGRYAIPQVRFGVAPDALAHWTLPRLVGTAVAAELLLTGASFSAQRAVETGLANRCLP
ECCCCEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
AGKVLGAALRMAHDIATNVAPESAALTKRLLWDAQMTGMSAAEVAARETADHLRLMGSQD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
AAEGPRAFIDGRPPRWAGQR
CCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA