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The map label for this gene is nth [H]

Identifier: 169824416

GI number: 169824416

Start: 781907

End: 782533

Strand: Direct

Name: nth [H]

Synonym: FMG_0719

Alternate gene names: 169824416

Gene position: 781907-782533 (Clockwise)

Preceding gene: 169824415

Following gene: 169824417

Centisome position: 43.5

GC content: 29.03

Gene sequence:

>627_bases
ATGAGTTACGACAAAATTAACAAAATTTTAGATGATTTAGATAGTTTATATCCTGATGCAAAAGCAGGGCTTGATTTTAC
AACACCTTTTGAATTATTGATTGCGACTATTTTATCAGCACAATGTACAGATGTTAGAGTAAATAAAGTTACAGCAGTTT
TATTCAAAGAACACAATACTCCTAAATCAATATTAGATTTAGGAATAGATGGATTAACCAAATATATCAAAAGCTGCGGA
CTCTACAAAACAAAATCAAAAAATATAATAAACACATGTAATGTTTTATATCATGATTATGATTCAAAAGTTCCTGATAA
TATTGAAGAATTAATGAAACTACCTGGAGTTGGAAGAAAAACTGCAAATGTAGTTGTTAGCAATGCTTTTGATACTCCTG
CAATCGCAGTAGATACTCATGTTTTTAGAGTAACTAATAGAATAGGAATAGTTAATGAAAAAGATGTATTAAGTACTGAA
AAGGCGTTAATGAGGGTAATACCAAAAGAAAGATGGTCAAAAAGTCATCACTTATTTATTTGGCATGGAAGAAATATATG
TAAGGCTAGAAATCCTAAGTGCGAAGAATGTATATTGAATGACAGATGTAAATTTTACAACTCATAA

Upstream 100 bases:

>100_bases
ATTTTTAACGAAAGACTGAAAGACAGGCCAATTATTTTGGAAACGCCTAATGACTTAGATGGATATAAAATAGAAATTGA
AGAAATAAGGAGAAATTTTA

Downstream 100 bases:

>100_bases
TAAAAAAACTATTGACATAAATCTTTTTTGATGTTATTATAGTAAAAATTAATCAAAGGAGATAGTTATGTTAAAAAATA
GAAATTTAATTAACGCGGAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSYDKINKILDDLDSLYPDAKAGLDFTTPFELLIATILSAQCTDVRVNKVTAVLFKEHNTPKSILDLGIDGLTKYIKSCG
LYKTKSKNIINTCNVLYHDYDSKVPDNIEELMKLPGVGRKTANVVVSNAFDTPAIAVDTHVFRVTNRIGIVNEKDVLSTE
KALMRVIPKERWSKSHHLFIWHGRNICKARNPKCEECILNDRCKFYNS

Sequences:

>Translated_208_residues
MSYDKINKILDDLDSLYPDAKAGLDFTTPFELLIATILSAQCTDVRVNKVTAVLFKEHNTPKSILDLGIDGLTKYIKSCG
LYKTKSKNIINTCNVLYHDYDSKVPDNIEELMKLPGVGRKTANVVVSNAFDTPAIAVDTHVFRVTNRIGIVNEKDVLSTE
KALMRVIPKERWSKSHHLFIWHGRNICKARNPKCEECILNDRCKFYNS
>Mature_207_residues
SYDKINKILDDLDSLYPDAKAGLDFTTPFELLIATILSAQCTDVRVNKVTAVLFKEHNTPKSILDLGIDGLTKYIKSCGL
YKTKSKNIINTCNVLYHDYDSKVPDNIEELMKLPGVGRKTANVVVSNAFDTPAIAVDTHVFRVTNRIGIVNEKDVLSTEK
ALMRVIPKERWSKSHHLFIWHGRNICKARNPKCEECILNDRCKFYNS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=94, Evalue=9e-20,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=48.2926829268293, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=34.8314606741573, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324530, Length=193, Percent_Identity=31.0880829015544, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319304, Length=179, Percent_Identity=30.1675977653631, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=33.8888888888889, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23577; Mature: 23446

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDKINKILDDLDSLYPDAKAGLDFTTPFELLIATILSAQCTDVRVNKVTAVLFKEHNT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHEEEEEECCCC
PKSILDLGIDGLTKYIKSCGLYKTKSKNIINTCNVLYHDYDSKVPDNIEELMKLPGVGRK
HHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCC
TANVVVSNAFDTPAIAVDTHVFRVTNRIGIVNEKDVLSTEKALMRVIPKERWSKSHHLFI
HHHEEEECCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHCCCCCCEEE
WHGRNICKARNPKCEECILNDRCKFYNS
EECCCCCCCCCCCHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SYDKINKILDDLDSLYPDAKAGLDFTTPFELLIATILSAQCTDVRVNKVTAVLFKEHNT
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHEEEEEECCCC
PKSILDLGIDGLTKYIKSCGLYKTKSKNIINTCNVLYHDYDSKVPDNIEELMKLPGVGRK
HHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCC
TANVVVSNAFDTPAIAVDTHVFRVTNRIGIVNEKDVLSTEKALMRVIPKERWSKSHHLFI
HHHEEEECCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHCCCCCCEEE
WHGRNICKARNPKCEECILNDRCKFYNS
EECCCCCCCCCCCHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]