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The map label for this gene is nfo

Identifier: 169824415

GI number: 169824415

Start: 781093

End: 781926

Strand: Direct

Name: nfo

Synonym: FMG_0718

Alternate gene names: 169824415

Gene position: 781093-781926 (Clockwise)

Preceding gene: 169824412

Following gene: 169824416

Centisome position: 43.45

GC content: 29.26

Gene sequence:

>834_bases
ATGAACAGAATAGGTTGTCATTTATCTACTTCGAAAGGACTTCACAAAACAATAGAACAATGTCTAGAAATAAATGCAGA
TACTTTTCAATTTTTTCCTCGAAACCCAAGAGGATCTAAGTCAAGGTTAATTCCTAAAAAAGAAATTGATAAATTCTTAG
AACTAAGGAAGATCCATAATATAAACAAAATTGTATGTCATGGAGCATATACCATGAATTTATGTAGTGATAGAGAAGAT
TTAAGAAAATTGGCAGTCAAACTTATCAATGAAGATATGAAAAAAATTCAGGACATGCGTATAAATCATTATGTTCTTCA
TCCAGGAAGCCATAAAAATCAAGGGATAGAAGAAGGTCTAAAATTAATCGTTGAAGGTATAAACAAAGTTGATGTTTCTA
ATGGTCAAATGATATGTATCGAAACTATGAGTGGTAAAGGTAGTGAGCTTGGATGTGATATAGACCAAATAGCATATATT
ATAAATAACGCCAGCATTCCTTTATATGTTTGTATAGATACTTGCCATTTATTTAGTTCTGGAATTAGATTAGATAATTT
TGATGATTATCTTGACGAATTTGATAAGAAAATTGGAATTGACAAGATAAAAGTAATTCATTGTAATGATTCTATGATGC
CTTTTGGAGCAAATAAAGATAGACACGAGAAGTTTGGGAAAGGATTAATTGGAGAACAAGATTTATTTAATGTAATTTTT
AACGAAAGACTGAAAGACAGGCCAATTATTTTGGAAACGCCTAATGACTTAGATGGATATAAAATAGAAATTGAAGAAAT
AAGGAGAAATTTTAATGAGTTACGACAAAATTAA

Upstream 100 bases:

>100_bases
ACATTTTTTTTGTTAATATTAAACATGTCTCACTTCCTCATGTAGTATTATAACATACTTAAATAGCCAGGTGTGATATA
ATAATGACTTGGAGGTTATT

Downstream 100 bases:

>100_bases
CAAAATTTTAGATGATTTAGATAGTTTATATCCTGATGCAAAAGCAGGGCTTGATTTTACAACACCTTTTGAATTATTGA
TTGCGACTATTTTATCAGCA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNRIGCHLSTSKGLHKTIEQCLEINADTFQFFPRNPRGSKSRLIPKKEIDKFLELRKIHNINKIVCHGAYTMNLCSDRED
LRKLAVKLINEDMKKIQDMRINHYVLHPGSHKNQGIEEGLKLIVEGINKVDVSNGQMICIETMSGKGSELGCDIDQIAYI
INNASIPLYVCIDTCHLFSSGIRLDNFDDYLDEFDKKIGIDKIKVIHCNDSMMPFGANKDRHEKFGKGLIGEQDLFNVIF
NERLKDRPIILETPNDLDGYKIEIEEIRRNFNELRQN

Sequences:

>Translated_277_residues
MNRIGCHLSTSKGLHKTIEQCLEINADTFQFFPRNPRGSKSRLIPKKEIDKFLELRKIHNINKIVCHGAYTMNLCSDRED
LRKLAVKLINEDMKKIQDMRINHYVLHPGSHKNQGIEEGLKLIVEGINKVDVSNGQMICIETMSGKGSELGCDIDQIAYI
INNASIPLYVCIDTCHLFSSGIRLDNFDDYLDEFDKKIGIDKIKVIHCNDSMMPFGANKDRHEKFGKGLIGEQDLFNVIF
NERLKDRPIILETPNDLDGYKIEIEEIRRNFNELRQN
>Mature_277_residues
MNRIGCHLSTSKGLHKTIEQCLEINADTFQFFPRNPRGSKSRLIPKKEIDKFLELRKIHNINKIVCHGAYTMNLCSDRED
LRKLAVKLINEDMKKIQDMRINHYVLHPGSHKNQGIEEGLKLIVEGINKVDVSNGQMICIETMSGKGSELGCDIDQIAYI
INNASIPLYVCIDTCHLFSSGIRLDNFDDYLDEFDKKIGIDKIKVIHCNDSMMPFGANKDRHEKFGKGLIGEQDLFNVIF
NERLKDRPIILETPNDLDGYKIEIEEIRRNFNELRQN

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=283, Percent_Identity=31.8021201413428, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531193, Length=262, Percent_Identity=31.2977099236641, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322735, Length=276, Percent_Identity=32.6086956521739, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_FINM2 (B0S196)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001692026.1
- ProteinModelPortal:   B0S196
- SMR:   B0S196
- GeneID:   6019930
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0718
- HOGENOM:   HBG565018
- OMA:   QIALETM
- BioCyc:   FMAG334413:FMG_0718-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31892; Mature: 31892

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIGCHLSTSKGLHKTIEQCLEINADTFQFFPRNPRGSKSRLIPKKEIDKFLELRKIHN
CCCCCCEECCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCC
INKIVCHGAYTMNLCSDREDLRKLAVKLINEDMKKIQDMRINHYVLHPGSHKNQGIEEGL
CCCEEEECCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHH
KLIVEGINKVDVSNGQMICIETMSGKGSELGCDIDQIAYIINNASIPLYVCIDTCHLFSS
HHHHHHHHHEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHC
GIRLDNFDDYLDEFDKKIGIDKIKVIHCNDSMMPFGANKDRHEKFGKGLIGEQDLFNVIF
CCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHH
NERLKDRPIILETPNDLDGYKIEIEEIRRNFNELRQN
HHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRIGCHLSTSKGLHKTIEQCLEINADTFQFFPRNPRGSKSRLIPKKEIDKFLELRKIHN
CCCCCCEECCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCC
INKIVCHGAYTMNLCSDREDLRKLAVKLINEDMKKIQDMRINHYVLHPGSHKNQGIEEGL
CCCEEEECCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHH
KLIVEGINKVDVSNGQMICIETMSGKGSELGCDIDQIAYIINNASIPLYVCIDTCHLFSS
HHHHHHHHHEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHC
GIRLDNFDDYLDEFDKKIGIDKIKVIHCNDSMMPFGANKDRHEKFGKGLIGEQDLFNVIF
CCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHH
NERLKDRPIILETPNDLDGYKIEIEEIRRNFNELRQN
HHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA