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The map label for this gene is pyrB

Identifier: 169824417

GI number: 169824417

Start: 782601

End: 783521

Strand: Direct

Name: pyrB

Synonym: FMG_0720

Alternate gene names: 169824417

Gene position: 782601-783521 (Clockwise)

Preceding gene: 169824416

Following gene: 169824418

Centisome position: 43.54

GC content: 30.94

Gene sequence:

>921_bases
ATGTTAAAAAATAGAAATTTAATTAACGCGGATGATTTTAATGTAGAAGAGATAAACGAAATATTAAATTTAGCAGAAGA
AATTATAAAGAGTCCTTCTGATTTTTCTAATTTATGTAATGGAAAAATCTTAGGGACTCTTTTTTTTGAGCCTTCAACAA
GAACTAGATTAAGTTTTGAGTCAGCAATACATAGATTAGGCGGTGATTGCATAGGATTTTCCGAAAGCGCAAGTTCATCT
ACATCAAAGGGTGAATCTTTGGCTGATACAATAAGAACTGTATCGAATTATACAGATATAATAGCAATGAGAAACCCAAA
AGAAGGTTCAGCAGTATTAGCTAGTTCATATGCAGAAGTTCCATTGATAAATGCAGGAGATGGCGGACATCAACATCCCA
CTCAAACATTGACAGACTTGCTAACAATTTGGATGACAAAGAAAAAATTAGACAATATGACTATAGGATTGTGTGGGGAT
TTGAAATTTGGTAGAACAGTGCATTCTTTAATAAAAGCTATGTCAAGATATGAAAACAACAAATTCATATTAATTTCACC
AGAAGAACTTCAAGTACCAGATTATATTAAAATGTTTTTAAAATCTAAAAATATAGAATTCAAAGAGGTTGAAAAAATGG
AAGACGTAATCGGAGAGCTTGATGTTTTATATATGACAAGAGTTCAAAAAGAAAGATTCTTTAATGAAGCAGATTATGTT
AGATTAAAAGATTCATATATTTTAGATAATGATAAAATGAAATTAGCAACTGAAGACTTAGCTGTACTTCATCCGTTGCC
AAGAGTGAATGAAATAGCTACTGAAGTTGATAGCGATCCAAGGGCCGTTTATTTCAAACAAGTTAGATACGGCGTAATTG
TTAGAATGGCATTAATATTAAAATTATTAGGGGTGAGATAA

Upstream 100 bases:

>100_bases
TTGAATGACAGATGTAAATTTTACAACTCATAATAAAAAAACTATTGACATAAATCTTTTTTGATGTTATTATAGTAAAA
ATTAATCAAAGGAGATAGTT

Downstream 100 bases:

>100_bases
TGTTAAACATTAATAGTATTGAAAAAGGTATTGTAATTGATCATATTAAATGTGGAATGGGATATAAAATATTTCAACTT
TTAAAGTTAGATAAAGCGGA

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLKNRNLINADDFNVEEINEILNLAEEIIKSPSDFSNLCNGKILGTLFFEPSTRTRLSFESAIHRLGGDCIGFSESASSS
TSKGESLADTIRTVSNYTDIIAMRNPKEGSAVLASSYAEVPLINAGDGGHQHPTQTLTDLLTIWMTKKKLDNMTIGLCGD
LKFGRTVHSLIKAMSRYENNKFILISPEELQVPDYIKMFLKSKNIEFKEVEKMEDVIGELDVLYMTRVQKERFFNEADYV
RLKDSYILDNDKMKLATEDLAVLHPLPRVNEIATEVDSDPRAVYFKQVRYGVIVRMALILKLLGVR

Sequences:

>Translated_306_residues
MLKNRNLINADDFNVEEINEILNLAEEIIKSPSDFSNLCNGKILGTLFFEPSTRTRLSFESAIHRLGGDCIGFSESASSS
TSKGESLADTIRTVSNYTDIIAMRNPKEGSAVLASSYAEVPLINAGDGGHQHPTQTLTDLLTIWMTKKKLDNMTIGLCGD
LKFGRTVHSLIKAMSRYENNKFILISPEELQVPDYIKMFLKSKNIEFKEVEKMEDVIGELDVLYMTRVQKERFFNEADYV
RLKDSYILDNDKMKLATEDLAVLHPLPRVNEIATEVDSDPRAVYFKQVRYGVIVRMALILKLLGVR
>Mature_306_residues
MLKNRNLINADDFNVEEINEILNLAEEIIKSPSDFSNLCNGKILGTLFFEPSTRTRLSFESAIHRLGGDCIGFSESASSS
TSKGESLADTIRTVSNYTDIIAMRNPKEGSAVLASSYAEVPLINAGDGGHQHPTQTLTDLLTIWMTKKKLDNMTIGLCGD
LKFGRTVHSLIKAMSRYENNKFILISPEELQVPDYIKMFLKSKNIEFKEVEKMEDVIGELDVLYMTRVQKERFFNEADYV
RLKDSYILDNDKMKLATEDLAVLHPLPRVNEIATEVDSDPRAVYFKQVRYGVIVRMALILKLLGVR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=40.327868852459, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI38788445, Length=319, Percent_Identity=27.5862068965517, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=45.3947368421053, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1790703, Length=164, Percent_Identity=32.9268292682927, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=38.5579937304075, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=41.6938110749186, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24642586, Length=308, Percent_Identity=41.5584415584416, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_FINM2 (B0S198)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001692028.1
- ProteinModelPortal:   B0S198
- SMR:   B0S198
- GeneID:   6019770
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0720
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- BioCyc:   FMAG334413:FMG_0720-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34568; Mature: 34568

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNRNLINADDFNVEEINEILNLAEEIIKSPSDFSNLCNGKILGTLFFEPSTRTRLSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCHHHHHH
SAIHRLGGDCIGFSESASSSTSKGESLADTIRTVSNYTDIIAMRNPKEGSAVLASSYAEV
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCCC
PLINAGDGGHQHPTQTLTDLLTIWMTKKKLDNMTIGLCGDLKFGRTVHSLIKAMSRYENN
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
KFILISPEELQVPDYIKMFLKSKNIEFKEVEKMEDVIGELDVLYMTRVQKERFFNEADYV
CEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
RLKDSYILDNDKMKLATEDLAVLHPLPRVNEIATEVDSDPRAVYFKQVRYGVIVRMALIL
EEECCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KLLGVR
HHHCCC
>Mature Secondary Structure
MLKNRNLINADDFNVEEINEILNLAEEIIKSPSDFSNLCNGKILGTLFFEPSTRTRLSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCHHHHHH
SAIHRLGGDCIGFSESASSSTSKGESLADTIRTVSNYTDIIAMRNPKEGSAVLASSYAEV
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCCC
PLINAGDGGHQHPTQTLTDLLTIWMTKKKLDNMTIGLCGDLKFGRTVHSLIKAMSRYENN
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
KFILISPEELQVPDYIKMFLKSKNIEFKEVEKMEDVIGELDVLYMTRVQKERFFNEADYV
CEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
RLKDSYILDNDKMKLATEDLAVLHPLPRVNEIATEVDSDPRAVYFKQVRYGVIVRMALIL
EEECCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KLLGVR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA