Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 167625671

GI number: 167625671

Start: 4575155

End: 4576042

Strand: Reverse

Name: pleD [H]

Synonym: Shal_3766

Alternate gene names: 167625671

Gene position: 4576042-4575155 (Counterclockwise)

Preceding gene: 167625672

Following gene: 167625668

Centisome position: 87.55

GC content: 38.63

Gene sequence:

>888_bases
ATGATAGAGGAAGACGTCAATCTGCTGAATGGGCTGCGTGCAGCATTAGATCACGTGCAGTCTTTTGTATATATTAAGAA
CTGCAAATTAGAGTATGTATACGCAAACAAGCACACTCTTGATCTTTTTGGATGTACTGCTGAAAATTTGAACTTAGCTG
ATGACTCTAACTTTTTCTCACCTGATATAGTGCCAATTCTAAGAGCCGTTGATCTAAGAGTTTTATCTGGAGAGTCCACA
AGAGAAGAGGTTATTGTTGACGATGGACATCCGGAAAAACGAATTTATCTTAACGTAAAAACGCCAGTTTATTGTGATAG
CGATCCTGAGTGCATCATAGGCATTTTAGGTATATCTACGGACATTACTTTCGCGAAATTACTTGAAGAAAAAGCTCTTA
AGCTAGCGAAAACTGACGTACTAACGGGATTGGCAAACCGATTGGAGCTGGATTCAGTACTTAGTTATGAAATTGAAAGA
CTCAAACGCTTTCAACATCCTTTAAGTATCATCATGTTAGATCTCGATCATTTTAAGACAGTGAATGATAATTATGGTCA
CCTACTTGGGGATAAGTGTTTGATAAATATCGCCAAAATGATGAAAAATAATTTCCGCTTAATTGATACTGTCGGTCGCT
GGGGAGGGGAAGAGTTCCTTATTATATGCCCCGAAACAGACTTTAATGGGGCTATAAAGCTGGCGGAGAAATTTAGACGT
CTTATTGAAGAGCATATTTTCCCAGAAGTTAAACGCATAACCGGAAGTCTTGGGGTAACCTCGTTTATTGAGGGCGATAC
TCATGAAACTTTAGTCAATAGAGCCGATAAGGCTTTATATAAAGCTAAAAAGGCTGGAAGAAATAGAGTTGAAGCTTTTG
AGGCGTGA

Upstream 100 bases:

>100_bases
TAAGCGCATTATCGGGCTGCTATTAAATATACCGCTGAAGTTAAATTTTATGGAATATTAATTTCAATTTTCCAAGTTTG
ATGTCGTTAAGGATAGACAA

Downstream 100 bases:

>100_bases
GTAAAATAGTGGGGTTAGAGTAAAGTCTCTCTCCCCAAATAATCCGCAGTCCATAACCAAACTTTTTGCCGCTCAAGCAG
TAGGCTTATCTTTTCGTCAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MIEEDVNLLNGLRAALDHVQSFVYIKNCKLEYVYANKHTLDLFGCTAENLNLADDSNFFSPDIVPILRAVDLRVLSGEST
REEVIVDDGHPEKRIYLNVKTPVYCDSDPECIIGILGISTDITFAKLLEEKALKLAKTDVLTGLANRLELDSVLSYEIER
LKRFQHPLSIIMLDLDHFKTVNDNYGHLLGDKCLINIAKMMKNNFRLIDTVGRWGGEEFLIICPETDFNGAIKLAEKFRR
LIEEHIFPEVKRITGSLGVTSFIEGDTHETLVNRADKALYKAKKAGRNRVEAFEA

Sequences:

>Translated_295_residues
MIEEDVNLLNGLRAALDHVQSFVYIKNCKLEYVYANKHTLDLFGCTAENLNLADDSNFFSPDIVPILRAVDLRVLSGEST
REEVIVDDGHPEKRIYLNVKTPVYCDSDPECIIGILGISTDITFAKLLEEKALKLAKTDVLTGLANRLELDSVLSYEIER
LKRFQHPLSIIMLDLDHFKTVNDNYGHLLGDKCLINIAKMMKNNFRLIDTVGRWGGEEFLIICPETDFNGAIKLAEKFRR
LIEEHIFPEVKRITGSLGVTSFIEGDTHETLVNRADKALYKAKKAGRNRVEAFEA
>Mature_295_residues
MIEEDVNLLNGLRAALDHVQSFVYIKNCKLEYVYANKHTLDLFGCTAENLNLADDSNFFSPDIVPILRAVDLRVLSGEST
REEVIVDDGHPEKRIYLNVKTPVYCDSDPECIIGILGISTDITFAKLLEEKALKLAKTDVLTGLANRLELDSVLSYEIER
LKRFQHPLSIIMLDLDHFKTVNDNYGHLLGDKCLINIAKMMKNNFRLIDTVGRWGGEEFLIICPETDFNGAIKLAEKFRR
LIEEHIFPEVKRITGSLGVTSFIEGDTHETLVNRADKALYKAKKAGRNRVEAFEA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=38.2513661202186, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=36.5591397849462, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=42.3312883435583, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=32.967032967033, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=34.3949044585987, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=29.9465240641711, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787802, Length=202, Percent_Identity=34.1584158415842, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=32.3170731707317, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=184, Percent_Identity=30.9782608695652, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=152, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=34.9593495934959, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=30.3030303030303, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33403; Mature: 33403

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEDVNLLNGLRAALDHVQSFVYIKNCKLEYVYANKHTLDLFGCTAENLNLADDSNFFS
CCCHHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
PDIVPILRAVDLRVLSGESTREEVIVDDGHPEKRIYLNVKTPVYCDSDPECIIGILGIST
CHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCEEEEEECCCEEECCCCCEEEEEEECCC
DITFAKLLEEKALKLAKTDVLTGLANRLELDSVLSYEIERLKRFQHPLSIIMLDLDHFKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCC
VNDNYGHLLGDKCLINIAKMMKNNFRLIDTVGRWGGEEFLIICPETDFNGAIKLAEKFRR
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEECCCCCCHHHHHHHHHHH
LIEEHIFPEVKRITGSLGVTSFIEGDTHETLVNRADKALYKAKKAGRNRVEAFEA
HHHHHHCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MIEEDVNLLNGLRAALDHVQSFVYIKNCKLEYVYANKHTLDLFGCTAENLNLADDSNFFS
CCCHHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
PDIVPILRAVDLRVLSGESTREEVIVDDGHPEKRIYLNVKTPVYCDSDPECIIGILGIST
CHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCEEEEEECCCEEECCCCCEEEEEEECCC
DITFAKLLEEKALKLAKTDVLTGLANRLELDSVLSYEIERLKRFQHPLSIIMLDLDHFKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCC
VNDNYGHLLGDKCLINIAKMMKNNFRLIDTVGRWGGEEFLIICPETDFNGAIKLAEKFRR
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEECCCCCCHHHHHHHHHHH
LIEEHIFPEVKRITGSLGVTSFIEGDTHETLVNRADKALYKAKKAGRNRVEAFEA
HHHHHHCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA