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The map label for this gene is cysS

Identifier: 167624689

GI number: 167624689

Start: 3363701

End: 3365080

Strand: Reverse

Name: cysS

Synonym: Shal_2771

Alternate gene names: 167624689

Gene position: 3365080-3363701 (Counterclockwise)

Preceding gene: 167624693

Following gene: 167624687

Centisome position: 64.38

GC content: 45.36

Gene sequence:

>1380_bases
ATGTTGAAGCTTTACAATAGTCTTACCCGCCAAAAAGAGCAGTTTGTGCCATTACAGCCAGGAAAGGTTGGCATGTATGT
GTGTGGGGTGACCATCTATGACTTATGTCATATTGGCCATGGGCGTACATTTGTATCTTTCGACATGATTGTTAGATACC
TAAGATACAGCGGATATGACGTTAATTTTCAACGTAATATTACCGACGTCGATGACAAAATTATCAAACGCGCAAATGAA
AACAATGAGAGTTGTGATTCATTAACTGAGCGTTTAATTGCCGAAATGCACAAAGATTTCGATTCATTGAATATGGCACG
TCCAGACAATGAACCTCGTGCCACACAGCATATTCCTGAAATCATTGATATGGTCGAAAGACTGATTGAACGTGATCATG
CCTACGTTGCGTCAAGTGGTGATGTGCTGTTTAGCGTATCCTCATTCCCTGAGTATGGCCGTTTATCTGGGCAGAATCTT
GAGCAGCTACAAGCCGGTGCGCGCGTGGAAGTTGAAGATACTAAGCGCGATCCAATGGACTTTGTACTTTGGAAGATGTC
TAAGCCGGGCGAGCCGACTTGGGAGTCGCCTTGGGGACCGGGTCGTCCAGGTTGGCATATCGAATGTTCAGCAATGAACA
GCAAGCACTTAGGTAACCATTTCGATATCCATGGTGGCGGCAGCGATCTGCAGTTCCCACACCATGAAAATGAGATCGCT
CAGTCATGCTGCGCCCACGATACGCCATATGTAAACTACTGGATGCATACGGGTATGGTGATGATTGATAAAGAGAAGAT
GTCAAAGTCTTTAAATAACTTCTTTACCATTCGCGATGTATTAGCTCATTACGACGCCGCGACAGTACGTTACTTCCTGT
TATCGGGTCATTACCGTAGCCAGCTTAACTACTCTGAAGATAACTTAAAGCAAGCTAAGGCTGCACTAGAGCGTATTTAC
ACCGCGCTTAAAGACTTAGACTTAAGCGTTGAAGCGGCGCCTGCCCAAGAGTTTGTGGCTAAGTTTAAGACGGCGATGGA
TGATGACTTCAATACGCCTGAAGCTTATTCGGTATTGTTTGATATGGTGCGTGAGATCAACCGACTAAAAGCAACTGATA
TGGCTAAAGCGTCAGCTTTAGGTGTCGCGCTTAAAGAACTCGCCGATGTGCTAGGTATTTTGGGTCAAGACGTTGATACC
TTCTTTAAAGGTGAAGGCAGTGACGATGAAGTGGCTGAAATTGAAGCCTTAATCGTTGAACGTAACCGTGCTCGTGCTGA
AAAAGACTGGCCTGCCGCTGACGTTGCACGCGATGGCTTAAATGCTTTGGGTGTGATTTTAGAAGATGGTCCAGAAGGCA
CCACTTGGCGTAAGAAGTAA

Upstream 100 bases:

>100_bases
TTTTGCGGGCTTTGTGCACGAAAACAGCTTGGAACAGCGACTTTTGCAGTGGTAGACTGAACAACTTATTTCTAAATATA
GCCGTTGAAGAGAATAATCA

Downstream 100 bases:

>100_bases
TTCTTAGCAATAAGTCATACTGACATTATGTTCCAGACACAAAAAAGCCTGCTTATGCAGGCTTTTTTGTGTCTGGAACA
CTTATGCCACACCCCATGGA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLKLYNSLTRQKEQFVPLQPGKVGMYVCGVTIYDLCHIGHGRTFVSFDMIVRYLRYSGYDVNFQRNITDVDDKIIKRANE
NNESCDSLTERLIAEMHKDFDSLNMARPDNEPRATQHIPEIIDMVERLIERDHAYVASSGDVLFSVSSFPEYGRLSGQNL
EQLQAGARVEVEDTKRDPMDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSKHLGNHFDIHGGGSDLQFPHHENEIA
QSCCAHDTPYVNYWMHTGMVMIDKEKMSKSLNNFFTIRDVLAHYDAATVRYFLLSGHYRSQLNYSEDNLKQAKAALERIY
TALKDLDLSVEAAPAQEFVAKFKTAMDDDFNTPEAYSVLFDMVREINRLKATDMAKASALGVALKELADVLGILGQDVDT
FFKGEGSDDEVAEIEALIVERNRARAEKDWPAADVARDGLNALGVILEDGPEGTTWRKK

Sequences:

>Translated_459_residues
MLKLYNSLTRQKEQFVPLQPGKVGMYVCGVTIYDLCHIGHGRTFVSFDMIVRYLRYSGYDVNFQRNITDVDDKIIKRANE
NNESCDSLTERLIAEMHKDFDSLNMARPDNEPRATQHIPEIIDMVERLIERDHAYVASSGDVLFSVSSFPEYGRLSGQNL
EQLQAGARVEVEDTKRDPMDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSKHLGNHFDIHGGGSDLQFPHHENEIA
QSCCAHDTPYVNYWMHTGMVMIDKEKMSKSLNNFFTIRDVLAHYDAATVRYFLLSGHYRSQLNYSEDNLKQAKAALERIY
TALKDLDLSVEAAPAQEFVAKFKTAMDDDFNTPEAYSVLFDMVREINRLKATDMAKASALGVALKELADVLGILGQDVDT
FFKGEGSDDEVAEIEALIVERNRARAEKDWPAADVARDGLNALGVILEDGPEGTTWRKK
>Mature_459_residues
MLKLYNSLTRQKEQFVPLQPGKVGMYVCGVTIYDLCHIGHGRTFVSFDMIVRYLRYSGYDVNFQRNITDVDDKIIKRANE
NNESCDSLTERLIAEMHKDFDSLNMARPDNEPRATQHIPEIIDMVERLIERDHAYVASSGDVLFSVSSFPEYGRLSGQNL
EQLQAGARVEVEDTKRDPMDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSKHLGNHFDIHGGGSDLQFPHHENEIA
QSCCAHDTPYVNYWMHTGMVMIDKEKMSKSLNNFFTIRDVLAHYDAATVRYFLLSGHYRSQLNYSEDNLKQAKAALERIY
TALKDLDLSVEAAPAQEFVAKFKTAMDDDFNTPEAYSVLFDMVREINRLKATDMAKASALGVALKELADVLGILGQDVDT
FFKGEGSDDEVAEIEALIVERNRARAEKDWPAADVARDGLNALGVILEDGPEGTTWRKK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=391, Percent_Identity=39.8976982097187, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI10835051, Length=250, Percent_Identity=40.4, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI62240992, Length=250, Percent_Identity=40.4, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI21269877, Length=250, Percent_Identity=40.4, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI303304987, Length=250, Percent_Identity=40.4, Blast_Score=192, Evalue=4e-49,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=69.6312364425163, Blast_Score=674, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509797, Length=246, Percent_Identity=40.650406504065, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17509795, Length=246, Percent_Identity=40.650406504065, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=334, Percent_Identity=35.3293413173653, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI19922218, Length=501, Percent_Identity=34.9301397205589, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19922364, Length=264, Percent_Identity=40.530303030303, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_SHEHH (B0TLV2)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674983.1
- ProteinModelPortal:   B0TLV2
- SMR:   B0TLV2
- GeneID:   5903081
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2771
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   SHAL458817:SHAL_2771-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51977; Mature: 51977

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLYNSLTRQKEQFVPLQPGKVGMYVCGVTIYDLCHIGHGRTFVSFDMIVRYLRYSGYD
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
VNFQRNITDVDDKIIKRANENNESCDSLTERLIAEMHKDFDSLNMARPDNEPRATQHIPE
CEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IIDMVERLIERDHAYVASSGDVLFSVSSFPEYGRLSGQNLEQLQAGARVEVEDTKRDPMD
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCHH
FVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSKHLGNHFDIHGGGSDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCHHHHH
QSCCAHDTPYVNYWMHTGMVMIDKEKMSKSLNNFFTIRDVLAHYDAATVRYFLLSGHYRS
HHHHCCCCCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
QLNYSEDNLKQAKAALERIYTALKDLDLSVEAAPAQEFVAKFKTAMDDDFNTPEAYSVLF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHH
DMVREINRLKATDMAKASALGVALKELADVLGILGQDVDTFFKGEGSDDEVAEIEALIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
RNRARAEKDWPAADVARDGLNALGVILEDGPEGTTWRKK
HHHHCCCCCCCHHHHHHHCHHHEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKLYNSLTRQKEQFVPLQPGKVGMYVCGVTIYDLCHIGHGRTFVSFDMIVRYLRYSGYD
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
VNFQRNITDVDDKIIKRANENNESCDSLTERLIAEMHKDFDSLNMARPDNEPRATQHIPE
CEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IIDMVERLIERDHAYVASSGDVLFSVSSFPEYGRLSGQNLEQLQAGARVEVEDTKRDPMD
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCHH
FVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSKHLGNHFDIHGGGSDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCHHHHH
QSCCAHDTPYVNYWMHTGMVMIDKEKMSKSLNNFFTIRDVLAHYDAATVRYFLLSGHYRS
HHHHCCCCCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
QLNYSEDNLKQAKAALERIYTALKDLDLSVEAAPAQEFVAKFKTAMDDDFNTPEAYSVLF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHH
DMVREINRLKATDMAKASALGVALKELADVLGILGQDVDTFFKGEGSDDEVAEIEALIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
RNRARAEKDWPAADVARDGLNALGVILEDGPEGTTWRKK
HHHHCCCCCCCHHHHHHHCHHHEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA