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The map label for this gene is tig

Identifier: 167624687

GI number: 167624687

Start: 3360357

End: 3361661

Strand: Reverse

Name: tig

Synonym: Shal_2769

Alternate gene names: 167624687

Gene position: 3361661-3360357 (Counterclockwise)

Preceding gene: 167624689

Following gene: 167624686

Centisome position: 64.31

GC content: 44.52

Gene sequence:

>1305_bases
ATGCAAGTTTCTGTTGAAACCACACAAGGCTTAGAGCGTCGCCTAACAATTTCTGTACCTGCTGAGCAGATCGAGAACAC
TGTTAAAGAAGCATTGAAAAGCGAAGCTAAGCGTGCCCGTATCCCAGGTTTCCGTCCAGGAAAAGTACCTGTAAGCGTTA
TTAATAAGCGTTATGGTAATGCAATTCGTCAAGATATCACTGGTGAAGTGATGCAGCGTAACTTCATTGAAGCAATCATT
GCTGAGAAGTTAAACCCTGCTGGCGCACCTACTTTTACTCCTGGTTCAACTGAAGGCGAAAACTTTGAGTTTGTTGCTAC
TTTTGAAATCTATCCAGAAGTTGAGCTAAAAGGTCTTGAGTCAATCACTGTTGAGCAGCCTACAGCTGAAGTAACAGAAG
CTGACGTTGATTCTATGATTGAAACGCTACGTAACCAGCATGCTACTTTTGAAGTAGCTGAGCGCGCAGCGGCTGAAGGC
GACAAAGCTAAAATTAACTTTGTTGGCTCAATCGACGGTGAAGAGTTTGAAGGCGGCAAAGCTGACGATTTCGAACTACA
ACTAGGCAGCGGCCGTATGATCCCAGGTTTCGAGTCTGGTGTTGAAGGTCACAAAGCGGGTGAAGAGTTCAATATCGAAG
TGACTTTCCCTGAAGATTACCACGCAGAAAACTTGAAGGGTAAAGTAGCAACTTTCGCTATTACTCTGAACGAAGTTCAA
GCGGCTAACCTTCCAGAAGTTAACGACGAGTTTGCTACACTATTCGGTATTACTGAAGGTGGCATCGACGCACTACGCGG
CGAAATCAGCAAGAACATGAGCCGTGAACTTGAACAAGCGCTTAAAGCGAATGTTAAAGAGCAAGTACTTAATGGTCTAG
TTGAGCAAAACGACATCGAATTGCCTGCAGCACTAATCGACGGTGAAGTTGAAGTATTGCGTAAGCAAGCTATGCAACGT
TTCGGCGACCAAGCTGCAAACATGCCTGAGCTACCAGCTGATCTATTCACTGAGCAAGCTGCACGTCGCGTTAAAGTAGG
TCTACTACTTGGCGAAGTGATCAAGGCAAACGAGCTAAAAGCTGAAGACGAACGTGTTCAAGCTTTGATCGCTTCTATGG
CTTCTGCATATGAAGATCCAAGTGAAGTTGTAGCATACTACAATGGTAACGAAGAACTTATGCAGAATATGCGCAACGTT
GCTCTAGAAGAGCAAGCGGTTGAAGCTTTGTTAAAAACTGCAACTTTGACTGAAAAAGCAGTCAACTTTGAAGAATTTAT
GAACAAGGCTACAGGTCGCGCGTAA

Upstream 100 bases:

>100_bases
GGGCATTTGGGTGAAATTTACGGATCAAGAAACGAATACCTGAATAGTTGATTTTTCGACTATTGCAAAGGACGATAGAC
ATATTTTTCGAGGTAAAAAA

Downstream 100 bases:

>100_bases
CCATCGCTTGACTTAAATGGCTATTAGCCATTTATAATGGCTCGTATGAGGTTCCTCATGCGGGCCATTTTTATTTAGGG
AAGTTTAATCATGCACAAAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGNAIRQDITGEVMQRNFIEAII
AEKLNPAGAPTFTPGSTEGENFEFVATFEIYPEVELKGLESITVEQPTAEVTEADVDSMIETLRNQHATFEVAERAAAEG
DKAKINFVGSIDGEEFEGGKADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
AANLPEVNDEFATLFGITEGGIDALRGEISKNMSRELEQALKANVKEQVLNGLVEQNDIELPAALIDGEVEVLRKQAMQR
FGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKANELKAEDERVQALIASMASAYEDPSEVVAYYNGNEELMQNMRNV
ALEEQAVEALLKTATLTEKAVNFEEFMNKATGRA

Sequences:

>Translated_434_residues
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGNAIRQDITGEVMQRNFIEAII
AEKLNPAGAPTFTPGSTEGENFEFVATFEIYPEVELKGLESITVEQPTAEVTEADVDSMIETLRNQHATFEVAERAAAEG
DKAKINFVGSIDGEEFEGGKADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
AANLPEVNDEFATLFGITEGGIDALRGEISKNMSRELEQALKANVKEQVLNGLVEQNDIELPAALIDGEVEVLRKQAMQR
FGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKANELKAEDERVQALIASMASAYEDPSEVVAYYNGNEELMQNMRNV
ALEEQAVEALLKTATLTEKAVNFEEFMNKATGRA
>Mature_434_residues
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGNAIRQDITGEVMQRNFIEAII
AEKLNPAGAPTFTPGSTEGENFEFVATFEIYPEVELKGLESITVEQPTAEVTEADVDSMIETLRNQHATFEVAERAAAEG
DKAKINFVGSIDGEEFEGGKADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
AANLPEVNDEFATLFGITEGGIDALRGEISKNMSRELEQALKANVKEQVLNGLVEQNDIELPAALIDGEVEVLRKQAMQR
FGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKANELKAEDERVQALIASMASAYEDPSEVVAYYNGNEELMQNMRNV
ALEEQAVEALLKTATLTEKAVNFEEFMNKATGRA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=430, Percent_Identity=60.2325581395349, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_SHEHH (B0TLV0)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674981.1
- ProteinModelPortal:   B0TLV0
- SMR:   B0TLV0
- GeneID:   5903210
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2769
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   SHAL458817:SHAL_2769-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47559; Mature: 47559

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGN
CEEECCHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AIRQDITGEVMQRNFIEAIIAEKLNPAGAPTFTPGSTEGENFEFVATFEIYPEVELKGLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCC
SITVEQPTAEVTEADVDSMIETLRNQHATFEVAERAAAEGDKAKINFVGSIDGEEFEGGK
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
ADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
CCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECHHC
AANLPEVNDEFATLFGITEGGIDALRGEISKNMSRELEQALKANVKEQVLNGLVEQNDIE
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCC
LPAALIDGEVEVLRKQAMQRFGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKANELK
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEDERVQALIASMASAYEDPSEVVAYYNGNEELMQNMRNVALEEQAVEALLKTATLTEKA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNFEEFMNKATGRA
CCHHHHHHHHCCCC
>Mature Secondary Structure
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGN
CEEECCHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AIRQDITGEVMQRNFIEAIIAEKLNPAGAPTFTPGSTEGENFEFVATFEIYPEVELKGLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCC
SITVEQPTAEVTEADVDSMIETLRNQHATFEVAERAAAEGDKAKINFVGSIDGEEFEGGK
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
ADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
CCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECHHC
AANLPEVNDEFATLFGITEGGIDALRGEISKNMSRELEQALKANVKEQVLNGLVEQNDIE
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCC
LPAALIDGEVEVLRKQAMQRFGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKANELK
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEDERVQALIASMASAYEDPSEVVAYYNGNEELMQNMRNVALEEQAVEALLKTATLTEKA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNFEEFMNKATGRA
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA