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The map label for this gene is sppA [H]

Identifier: 167623955

GI number: 167623955

Start: 2445141

End: 2446982

Strand: Reverse

Name: sppA [H]

Synonym: Shal_2029

Alternate gene names: 167623955

Gene position: 2446982-2445141 (Counterclockwise)

Preceding gene: 167623957

Following gene: 167623954

Centisome position: 46.82

GC content: 44.46

Gene sequence:

>1842_bases
ATGCCCACTAAACCCTCAATATTTAAAAGGATGTTCCTCCTTATATGGAATACCGTCAATGGACTAAGGAAGCTTTTTCT
TAATCTTATTTTCTTTGGCGTGATTGCTATTATCATTGTAAGTCTTACCACAGATGATGGGGTAGAAGTAGAAGATGGTT
CCGCGTTGGTACTCAATCTATCAGGAGTGATTGTTGATCAAAAGCGCCAAGTAGATCCCATCGAAGCCGCCATGAAAAGC
GGAAATGAGGCCGACGGTAGCGGTGAGATTTTATTGGCTGACGTGTTAACCGTTATTGAGAACGCCGCAACGGATACACG
GATCAACCAGATGGTGTTAGACTTAGGGATGCTACACGGCACTGGTATAAGCAAACTTCAGTCAATCGGCAATGCTATCG
AATCTTTCAAGGCCACAGGCAAGACCGTTGTGGCTAACGGTAATTGGTATGGTCAAAATCAATATTTCCTTGCCAGCTTT
GCCGATAAAGTTTATCTCAACCCACAAGGGAGTGTTGAAATTGAAGGCTTGGCCCGCTACCGCCTTTACTTCAAATCAGC
TTTAGACAAACTAAAAATCAATGCACACGTTTTCAGAGTCGGCACATTCAAGTCCGCAGTAGAGCCGTTTATTCGCGACG
ATATGTCTGATGCAGCTAAAGAAGCTAACTTAGTGCTATTAAACGATTTATGGCAGAGCTATGCTGATACTGTTGCCGCT
AACCGTGGTATTGATAGCAATAATTTATCACTAAGCGCAGATAATTACCTTGCCGAGCTCGATAAGGCTAACGGTAAATC
GGCTGAAATGGCGCTGAATATGAAATGGGTCGATGGCCTTAAAACCACCGATGAGTTTAGGCTTGTTATGGTTGATGCCG
TAGGTAAATCGGCGGATGGGGATTCTTATAAGCATATCGATTTTTATGATTATCTGTCGGTCACTCAAACTCACCCTATT
CTATCTCTAAACGACAAAGTCGGTATTATTGTTGCCAAGGGTAATATTCTCAATGGCTCACAACCTGCAGGCCAAATTGG
TGGTAAGAGTACTTCAGAGTTATTAAGACAAGCTCGCTTTGATGATTCAGTTAAAGCTGTCGTACTCCGTGTAGACAGCC
CAGGTGGTAGCGCCTTCGCTTCTGAAGAGATTAGACAAGAAGTGTTAGCGATAAAAGCAGCTAACAAGCCAATCATCGTC
AGCATGGGCAGTTATGCCGCATCGGGCGGTTACTGGATCTCAGCGAGTGCAGATTATATCTATGCAACCCCAACCACATT
AACGGGTTCCATCGGGATCTTTGGCATGGTGACCACGTTCGAAGACTCGTTAGCCAGCATTGGCGTTCACACTGATGGTG
TGGGCACATCAGAATGGGCTGGTTTTTCAGTCACCAAAGGTCTGTCTCCTCAAATTCAAGCGATTATTCAACGCCATATT
GAGCGCGGTTATCAAGATTTTATCTCATTGGTGGCATCAGAGCGCGATATGAGCCTAGATTATGTCGATAGCATTGCTCA
GGGACGGGTATGGACAGGACGTAAGGCATTAGAGTTAGGCCTTGTTGATGGCTTAGGAGAGCTTCAAGATGCCGTCACTA
AAGCGGCACAAATGGCCAAGCTTGAACAGTACGACACTGAAGTCATCGAAAGAGAGCTTTCTCCACAAGAGCAGTTTATT
CAAGAGATGTTAGCGTCAGCGTCGAGCTATATGCCGCAAAGTTTCGCTCAATCTAGTGTACTTGAAACGGTCTTGTCACA
GTGGTCGAGTGTGATTGATGAATTTAAGTCATTTGACGATCCAAACGGTGTTTACTTGTATTGCGATACCTGTAATTACT
AA

Upstream 100 bases:

>100_bases
AGCTCAATAAGCCTGCTAATAGATTCCTTTACAATTGAATTTTCCTTTCCTCGCAGATTTCAGTTAGCATTATGTCAATT
AAGCTAGAAAGGAGTGGCTA

Downstream 100 bases:

>100_bases
AAACCGTTATGCATAAATGTGAAGTACAGGTGTAGACCAACAAATGAGCCCAGCGAATCGCTGGGCTCATTCATTTCCCC
TATCAATCCACTGACTTTTG

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MPTKPSIFKRMFLLIWNTVNGLRKLFLNLIFFGVIAIIIVSLTTDDGVEVEDGSALVLNLSGVIVDQKRQVDPIEAAMKS
GNEADGSGEILLADVLTVIENAATDTRINQMVLDLGMLHGTGISKLQSIGNAIESFKATGKTVVANGNWYGQNQYFLASF
ADKVYLNPQGSVEIEGLARYRLYFKSALDKLKINAHVFRVGTFKSAVEPFIRDDMSDAAKEANLVLLNDLWQSYADTVAA
NRGIDSNNLSLSADNYLAELDKANGKSAEMALNMKWVDGLKTTDEFRLVMVDAVGKSADGDSYKHIDFYDYLSVTQTHPI
LSLNDKVGIIVAKGNILNGSQPAGQIGGKSTSELLRQARFDDSVKAVVLRVDSPGGSAFASEEIRQEVLAIKAANKPIIV
SMGSYAASGGYWISASADYIYATPTTLTGSIGIFGMVTTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAIIQRHI
ERGYQDFISLVASERDMSLDYVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAQMAKLEQYDTEVIERELSPQEQFI
QEMLASASSYMPQSFAQSSVLETVLSQWSSVIDEFKSFDDPNGVYLYCDTCNY

Sequences:

>Translated_613_residues
MPTKPSIFKRMFLLIWNTVNGLRKLFLNLIFFGVIAIIIVSLTTDDGVEVEDGSALVLNLSGVIVDQKRQVDPIEAAMKS
GNEADGSGEILLADVLTVIENAATDTRINQMVLDLGMLHGTGISKLQSIGNAIESFKATGKTVVANGNWYGQNQYFLASF
ADKVYLNPQGSVEIEGLARYRLYFKSALDKLKINAHVFRVGTFKSAVEPFIRDDMSDAAKEANLVLLNDLWQSYADTVAA
NRGIDSNNLSLSADNYLAELDKANGKSAEMALNMKWVDGLKTTDEFRLVMVDAVGKSADGDSYKHIDFYDYLSVTQTHPI
LSLNDKVGIIVAKGNILNGSQPAGQIGGKSTSELLRQARFDDSVKAVVLRVDSPGGSAFASEEIRQEVLAIKAANKPIIV
SMGSYAASGGYWISASADYIYATPTTLTGSIGIFGMVTTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAIIQRHI
ERGYQDFISLVASERDMSLDYVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAQMAKLEQYDTEVIERELSPQEQFI
QEMLASASSYMPQSFAQSSVLETVLSQWSSVIDEFKSFDDPNGVYLYCDTCNY
>Mature_612_residues
PTKPSIFKRMFLLIWNTVNGLRKLFLNLIFFGVIAIIIVSLTTDDGVEVEDGSALVLNLSGVIVDQKRQVDPIEAAMKSG
NEADGSGEILLADVLTVIENAATDTRINQMVLDLGMLHGTGISKLQSIGNAIESFKATGKTVVANGNWYGQNQYFLASFA
DKVYLNPQGSVEIEGLARYRLYFKSALDKLKINAHVFRVGTFKSAVEPFIRDDMSDAAKEANLVLLNDLWQSYADTVAAN
RGIDSNNLSLSADNYLAELDKANGKSAEMALNMKWVDGLKTTDEFRLVMVDAVGKSADGDSYKHIDFYDYLSVTQTHPIL
SLNDKVGIIVAKGNILNGSQPAGQIGGKSTSELLRQARFDDSVKAVVLRVDSPGGSAFASEEIRQEVLAIKAANKPIIVS
MGSYAASGGYWISASADYIYATPTTLTGSIGIFGMVTTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAIIQRHIE
RGYQDFISLVASERDMSLDYVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAQMAKLEQYDTEVIERELSPQEQFIQ
EMLASASSYMPQSFAQSSVLETVLSQWSSVIDEFKSFDDPNGVYLYCDTCNY

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=608, Percent_Identity=43.75, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1787527, Length=163, Percent_Identity=31.2883435582822, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66702; Mature: 66571

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKPSIFKRMFLLIWNTVNGLRKLFLNLIFFGVIAIIIVSLTTDDGVEVEDGSALVLNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCEEEEEE
SGVIVDQKRQVDPIEAAMKSGNEADGSGEILLADVLTVIENAATDTRINQMVLDLGMLHG
CEEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
TGISKLQSIGNAIESFKATGKTVVANGNWYGQNQYFLASFADKVYLNPQGSVEIEGLARY
CCHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEECCCEEEECCCCCEEEHHHHHH
RLYFKSALDKLKINAHVFRVGTFKSAVEPFIRDDMSDAAKEANLVLLNDLWQSYADTVAA
HHHHHHHHHHEEECEEEEEECCHHHHHHHHHHCCHHHHHHHCCEEEHHHHHHHHHHHHHH
NRGIDSNNLSLSADNYLAELDKANGKSAEMALNMKWVDGLKTTDEFRLVMVDAVGKSADG
CCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEEEEEHHCCCCCCCCEEEEEEEECCCCCCC
DSYKHIDFYDYLSVTQTHPILSLNDKVGIIVAKGNILNGSQPAGQIGGKSTSELLRQARF
CCEEECCHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
DDSVKAVVLRVDSPGGSAFASEEIRQEVLAIKAANKPIIVSMGSYAASGGYWISASADYI
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCEE
YATPTTLTGSIGIFGMVTTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAIIQRHI
EECCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHH
ERGYQDFISLVASERDMSLDYVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAQMAK
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCHHHEEHHHCCHHHHHHHHHHHHHHHH
LEQYDTEVIERELSPQEQFIQEMLASASSYMPQSFAQSSVLETVLSQWSSVIDEFKSFDD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PNGVYLYCDTCNY
CCCEEEEEECCCC
>Mature Secondary Structure 
PTKPSIFKRMFLLIWNTVNGLRKLFLNLIFFGVIAIIIVSLTTDDGVEVEDGSALVLNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCEEEEEE
SGVIVDQKRQVDPIEAAMKSGNEADGSGEILLADVLTVIENAATDTRINQMVLDLGMLHG
CEEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
TGISKLQSIGNAIESFKATGKTVVANGNWYGQNQYFLASFADKVYLNPQGSVEIEGLARY
CCHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEECCCEEEECCCCCEEEHHHHHH
RLYFKSALDKLKINAHVFRVGTFKSAVEPFIRDDMSDAAKEANLVLLNDLWQSYADTVAA
HHHHHHHHHHEEECEEEEEECCHHHHHHHHHHCCHHHHHHHCCEEEHHHHHHHHHHHHHH
NRGIDSNNLSLSADNYLAELDKANGKSAEMALNMKWVDGLKTTDEFRLVMVDAVGKSADG
CCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEEEEEHHCCCCCCCCEEEEEEEECCCCCCC
DSYKHIDFYDYLSVTQTHPILSLNDKVGIIVAKGNILNGSQPAGQIGGKSTSELLRQARF
CCEEECCHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
DDSVKAVVLRVDSPGGSAFASEEIRQEVLAIKAANKPIIVSMGSYAASGGYWISASADYI
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCEE
YATPTTLTGSIGIFGMVTTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAIIQRHI
EECCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHH
ERGYQDFISLVASERDMSLDYVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAQMAK
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCHHHEEHHHCCHHHHHHHHHHHHHHHH
LEQYDTEVIERELSPQEQFIQEMLASASSYMPQSFAQSSVLETVLSQWSSVIDEFKSFDD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PNGVYLYCDTCNY
CCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]