Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 167623954

GI number: 167623954

Start: 2443893

End: 2444909

Strand: Reverse

Name: ansA [H]

Synonym: Shal_2028

Alternate gene names: 167623954

Gene position: 2444909-2443893 (Counterclockwise)

Preceding gene: 167623955

Following gene: 167623950

Centisome position: 46.78

GC content: 46.31

Gene sequence:

>1017_bases
ATGACTAAACGATCTATCTACGTAGCCTACACTGGCGGCACCATTGGTATGCAAAAAACCGAACATGGCTTTGCCCCTGT
TGCCGACTTTCTTACAGGCTGCGTCAAATCTATGCCTGAGTTTTACCATGATGAAATGCCTGATTTTGTAATTAGTGAGT
ACACTCCGCTGATCGACTCGTCAAATATGAAACCGACCGATTGGCAGATGATCGCCGATGACATCAAAGCCAATTACGAT
AAGTATGACGGCTTTGTGATCCTCCATGGTACCGACACCATGGCATTTACCGCATCGGCTCTCTCCTTCATGCTACAAGG
CTTACAAAAGCCCGTTATCGTAACTGGCTCGCAAATTCCCCTTGCCCAGCTGAGATCAGACGGTCAAACAAACCTACTCA
ACGCCCTATATATAGCGGCAAACTATCCTGTTGCCGAAGTCTGCTTATTCTTTAACAACAAACTATTTCGCGGTAATCGA
ACCACTAAAGCCCATGCCGACGGCTTTAATGCCTTTGCATCGCCAAACTTCCCGCTGCTTTTAGAAGCCGGAATTAAGAT
CCGTTGGCATGCTGGTAAGCGCGCAAAAATTGATAATAATGTGTCTTTACAGGTTGCCAAGATAAGCCCGCAACCCATAG
GGGTTGTGACCCTTTACCCTGGTATCAATACCGAGATCTTTGCCAATATTTTGCAGCAGCCAGTTAAAGCCTTAATCTTA
CTGACCTTTGGTGTTGGCAATGCTCCACAAACCCCTGAATTACTCAAAACCCTAAAAGATGCACACGAACGCGGTATCGT
ACTCGTCAACTTAACTCAGTGCATGCAAGGTCGAGTCAATATGGAAGGATATGCTACAGGTAACGCTTTGGCAGAAGCTG
GTGTGATCAGCGGCTATGATATGACCACTGAAGCTGCGCTGACAAAATTACACTTCTTGCTATCTACGGATATGACACCC
GAGGAAATTCGCGTCGCGATGAAGACTTCTATCGCTGGCGAGTTAACCGCTGATTAA

Upstream 100 bases:

>100_bases
TACTTACCTCAGCTAATGACCCTTATTGTCTGCATCTCTATCCACTTTGGGTATATAATCCCTACATCGTGATTTAATCG
AAACTAAAAAACAAAATCAA

Downstream 100 bases:

>100_bases
CGAAGATGGATATAATAAAAGGGCCCTAAGCAGTGCATGCTTGGGGCCCTTTTTTGCGTTCAAGTTTTAGTTAAAGCTCT
TTCTCTTTAAACTCGGCGAT

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTKRSIYVAYTGGTIGMQKTEHGFAPVADFLTGCVKSMPEFYHDEMPDFVISEYTPLIDSSNMKPTDWQMIADDIKANYD
KYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIPLAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNR
TTKAHADGFNAFASPNFPLLLEAGIKIRWHAGKRAKIDNNVSLQVAKISPQPIGVVTLYPGINTEIFANILQQPVKALIL
LTFGVGNAPQTPELLKTLKDAHERGIVLVNLTQCMQGRVNMEGYATGNALAEAGVISGYDMTTEAALTKLHFLLSTDMTP
EEIRVAMKTSIAGELTAD

Sequences:

>Translated_338_residues
MTKRSIYVAYTGGTIGMQKTEHGFAPVADFLTGCVKSMPEFYHDEMPDFVISEYTPLIDSSNMKPTDWQMIADDIKANYD
KYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIPLAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNR
TTKAHADGFNAFASPNFPLLLEAGIKIRWHAGKRAKIDNNVSLQVAKISPQPIGVVTLYPGINTEIFANILQQPVKALIL
LTFGVGNAPQTPELLKTLKDAHERGIVLVNLTQCMQGRVNMEGYATGNALAEAGVISGYDMTTEAALTKLHFLLSTDMTP
EEIRVAMKTSIAGELTAD
>Mature_337_residues
TKRSIYVAYTGGTIGMQKTEHGFAPVADFLTGCVKSMPEFYHDEMPDFVISEYTPLIDSSNMKPTDWQMIADDIKANYDK
YDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIPLAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNRT
TKAHADGFNAFASPNFPLLLEAGIKIRWHAGKRAKIDNNVSLQVAKISPQPIGVVTLYPGINTEIFANILQQPVKALILL
TFGVGNAPQTPELLKTLKDAHERGIVLVNLTQCMQGRVNMEGYATGNALAEAGVISGYDMTTEAALTKLHFLLSTDMTPE
EIRVAMKTSIAGELTAD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=43.5393258426966, Blast_Score=269, Evalue=2e-72,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=68.3431952662722, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1789327, Length=277, Percent_Identity=28.158844765343, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=362, Percent_Identity=40.8839779005525, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=40.8839779005525, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=40.8839779005525, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17558278, Length=362, Percent_Identity=40.8839779005525, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24639609, Length=372, Percent_Identity=40.3225806451613, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24645530, Length=360, Percent_Identity=41.3888888888889, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36920; Mature: 36789

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRSIYVAYTGGTIGMQKTEHGFAPVADFLTGCVKSMPEFYHDEMPDFVISEYTPLIDS
CCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
SNMKPTDWQMIADDIKANYDKYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIP
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNRTTKAHADGFNAFASPNFPLL
HHHHHCCCCHHHEEEEEEECCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEE
LEAGIKIRWHAGKRAKIDNNVSLQVAKISPQPIGVVTLYPGINTEIFANILQQPVKALIL
EECCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHEE
LTFGVGNAPQTPELLKTLKDAHERGIVLVNLTQCMQGRVNMEGYATGNALAEAGVISGYD
EEECCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCEEECCCHHHHCCCCCCCC
MTTEAALTKLHFLLSTDMTPEEIRVAMKTSIAGELTAD
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure 
TKRSIYVAYTGGTIGMQKTEHGFAPVADFLTGCVKSMPEFYHDEMPDFVISEYTPLIDS
CCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
SNMKPTDWQMIADDIKANYDKYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIP
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNRTTKAHADGFNAFASPNFPLL
HHHHHCCCCHHHEEEEEEECCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEE
LEAGIKIRWHAGKRAKIDNNVSLQVAKISPQPIGVVTLYPGINTEIFANILQQPVKALIL
EECCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHEE
LTFGVGNAPQTPELLKTLKDAHERGIVLVNLTQCMQGRVNMEGYATGNALAEAGVISGYD
EEECCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCEEECCCHHHHCCCCCCCC
MTTEAALTKLHFLLSTDMTPEEIRVAMKTSIAGELTAD
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]