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The map label for this gene is purR [H]

Identifier: 167623933

GI number: 167623933

Start: 2421515

End: 2422504

Strand: Reverse

Name: purR [H]

Synonym: Shal_2007

Alternate gene names: 167623933

Gene position: 2422504-2421515 (Counterclockwise)

Preceding gene: 167623939

Following gene: 167623932

Centisome position: 46.35

GC content: 40.2

Gene sequence:

>990_bases
ATGAAGAAAATAAAGTTAGCTGATATCGCCAAGGTAGCAAATGTATCGTCGATAACCGTTAGTAGAGCGTTATCAACACC
TGAAAAGGTTAAACCTGCGACTTGTGAAAAGATCCAAAAGATCGCTCAAGAGATGGGCTATATTCCGAATCTTATGGCAA
AAGGATTAAAGTCGAAATCTAAAACCATAGGGGTTATCGTCCCAACTATCATCAACCCCTTCTTTGCAAGCGCAGTTAAA
TCCATTATTCGTAGCGCAAATAAGGCTGGCTATAACTGCCTGTTGTTCAGTTCAGATGAATCACAAAACATCGAGCAAAA
TGCGGTCAACACCCTCATCAGTTACAATATTGACGGTGTCATCATTGCGGTGATCTCTGAAGATGAACACTACACCCCCT
CATACTTTTCAGTCTTAAAAAAACTAGAGGTCCCCGTGGTATTGCTAGATAGACATATCGATGCGCCTCATGTTTGCGGC
GTCTATTTAGATAATGTCGATTCAGGTTATAAGCTTGGCAAAGAAGTGATATCAAACAACTTTCAACGAATACTCATCGT
ATCAGGCTCTGCCAACTCTAAGGTTGCGAATGCAAGATTAGCGGGCCTTAAGATGGCCGCTGAAGAAGTTAATTCAAATA
CTAGTATTGATATTATAAAAGCCGACTTTAATACCGAGCTGGCAAAGAATTTAGTGCAAACTTACCTTGAGCAACACAAA
CCTGATGCCATTATTGGTTTAAATAACCAGATAACCCTAGGAGCCTTAGAGGCCAGTGTAGAACATGATTACCAACTTGG
GGTTGATATTCATTTCTATAGTATCGATGAAATAAAGACTGCAAAGAACTTCGGCATTTCCATCCCCTGTATCAGACACG
ATGTAGAAGATTTTGGCTACCATGCGGTGACTCAACTAATTAGAGCAATTGAATCTGAAAAGTGCGACAAATTATCGGAT
ATCATCATTAGAGGCGCAGTCATAAAGTAG

Upstream 100 bases:

>100_bases
ATAACATTGGCTTGAGTACGAGTGGTAACAGAAAAAGTGGTACGCTCATTTTGCCGCAGACCTTATAAACAAAACGGTAA
AATGGTGCCGGGAACAAAAT

Downstream 100 bases:

>100_bases
TCACTTTGTGATAGTTCACATGAGCTCAGATCAATAACATCCGAAAGTGACTTTAAAGTGAAAAGCAGCAATCGAGATCC
TTCTATCAACACGAAAACAA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKIKLADIAKVANVSSITVSRALSTPEKVKPATCEKIQKIAQEMGYIPNLMAKGLKSKSKTIGVIVPTIINPFFASAVK
SIIRSANKAGYNCLLFSSDESQNIEQNAVNTLISYNIDGVIIAVISEDEHYTPSYFSVLKKLEVPVVLLDRHIDAPHVCG
VYLDNVDSGYKLGKEVISNNFQRILIVSGSANSKVANARLAGLKMAAEEVNSNTSIDIIKADFNTELAKNLVQTYLEQHK
PDAIIGLNNQITLGALEASVEHDYQLGVDIHFYSIDEIKTAKNFGISIPCIRHDVEDFGYHAVTQLIRAIESEKCDKLSD
IIIRGAVIK

Sequences:

>Translated_329_residues
MKKIKLADIAKVANVSSITVSRALSTPEKVKPATCEKIQKIAQEMGYIPNLMAKGLKSKSKTIGVIVPTIINPFFASAVK
SIIRSANKAGYNCLLFSSDESQNIEQNAVNTLISYNIDGVIIAVISEDEHYTPSYFSVLKKLEVPVVLLDRHIDAPHVCG
VYLDNVDSGYKLGKEVISNNFQRILIVSGSANSKVANARLAGLKMAAEEVNSNTSIDIIKADFNTELAKNLVQTYLEQHK
PDAIIGLNNQITLGALEASVEHDYQLGVDIHFYSIDEIKTAKNFGISIPCIRHDVEDFGYHAVTQLIRAIESEKCDKLSD
IIIRGAVIK
>Mature_329_residues
MKKIKLADIAKVANVSSITVSRALSTPEKVKPATCEKIQKIAQEMGYIPNLMAKGLKSKSKTIGVIVPTIINPFFASAVK
SIIRSANKAGYNCLLFSSDESQNIEQNAVNTLISYNIDGVIIAVISEDEHYTPSYFSVLKKLEVPVVLLDRHIDAPHVCG
VYLDNVDSGYKLGKEVISNNFQRILIVSGSANSKVANARLAGLKMAAEEVNSNTSIDIIKADFNTELAKNLVQTYLEQHK
PDAIIGLNNQITLGALEASVEHDYQLGVDIHFYSIDEIKTAKNFGISIPCIRHDVEDFGYHAVTQLIRAIESEKCDKLSD
IIIRGAVIK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=25.2941176470588, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787948, Length=308, Percent_Identity=24.025974025974, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=273, Percent_Identity=24.1758241758242, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=24.7588424437299, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790715, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI48994940, Length=187, Percent_Identity=29.9465240641711, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789068, Length=288, Percent_Identity=25.6944444444444, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1786540, Length=313, Percent_Identity=26.8370607028754, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787906, Length=278, Percent_Identity=25.5395683453237, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=25.7861635220126, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=25.8278145695364, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36046; Mature: 36046

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKLADIAKVANVSSITVSRALSTPEKVKPATCEKIQKIAQEMGYIPNLMAKGLKSKS
CCCCCHHHHHHHHCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
KTIGVIVPTIINPFFASAVKSIIRSANKAGYNCLLFSSDESQNIEQNAVNTLISYNIDGV
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHEECCCEE
IIAVISEDEHYTPSYFSVLKKLEVPVVLLDRHIDAPHVCGVYLDNVDSGYKLGKEVISNN
EEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCC
FQRILIVSGSANSKVANARLAGLKMAAEEVNSNTSIDIIKADFNTELAKNLVQTYLEQHK
CEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
PDAIIGLNNQITLGALEASVEHDYQLGVDIHFYSIDEIKTAKNFGISIPCIRHDVEDFGY
CCEEEECCCCEEEEEEHHCCCCCEEECEEEEEEEHHHHHHHHHCCCCCHHHHHCHHHHCH
HAVTQLIRAIESEKCDKLSDIIIRGAVIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKIKLADIAKVANVSSITVSRALSTPEKVKPATCEKIQKIAQEMGYIPNLMAKGLKSKS
CCCCCHHHHHHHHCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
KTIGVIVPTIINPFFASAVKSIIRSANKAGYNCLLFSSDESQNIEQNAVNTLISYNIDGV
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHEECCCEE
IIAVISEDEHYTPSYFSVLKKLEVPVVLLDRHIDAPHVCGVYLDNVDSGYKLGKEVISNN
EEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCC
FQRILIVSGSANSKVANARLAGLKMAAEEVNSNTSIDIIKADFNTELAKNLVQTYLEQHK
CEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
PDAIIGLNNQITLGALEASVEHDYQLGVDIHFYSIDEIKTAKNFGISIPCIRHDVEDFGY
CCEEEECCCCEEEEEEHHCCCCCEEECEEEEEEEHHHHHHHHHCCCCCHHHHHCHHHHCH
HAVTQLIRAIESEKCDKLSDIIIRGAVIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA