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The map label for this gene is gloA [H]

Identifier: 167623854

GI number: 167623854

Start: 2313136

End: 2313546

Strand: Reverse

Name: gloA [H]

Synonym: Shal_1924

Alternate gene names: 167623854

Gene position: 2313546-2313136 (Counterclockwise)

Preceding gene: 167623855

Following gene: 167623846

Centisome position: 44.26

GC content: 47.93

Gene sequence:

>411_bases
ATGGCACAACTATTACATACCATGCTCCGCGTTGGCAATCTTGAACGCAGTATCGAGTTTTACACTAAAGTCTTAGGCAT
GAAGCTACTTAGACAAAGCGAAAACAGCGAATATCAATACACCCTAGCTTTTGTTGGCTACGACGAAGAATCAACGGGAA
GCGCGGTGATTGAACTGACCTATAACTGGGGCACAGAGTGTTATGATCACGGTAACGCCTTTGGTCATATCGCTATTGGC
GAAGAGGATATCTATGCGCGCTGTGATGCTATCGCTGCAGCGGGTGGTAAAGTGATCCGCCCTGCAGGCCCTGTCGCAGG
CGGCAGCACCGAAATCGCCTTTGTGGAAGATCCTGATGGCTACAAAATTGAATTTATCCAGATGAGTTCTGCCGAAAAAG
GCTTAGGCTAA

Upstream 100 bases:

>100_bases
CTAAATAAATTGATCTCGCTCAATTTTTGCAAAGTGTATCTATCATCCATAATTTCGATATACTTCAGCCATCTGTTACC
CTATCACTTGGATATTTATC

Downstream 100 bases:

>100_bases
GTTTTAGTTCTTTTCATCCAATAATAAAAAAAGCGCCTTTCGGCGCTTTTTTTAGATTAAATAGCGATAGCTATTTAGAA
GTTCATGGTTACACCAGCAA

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MAQLLHTMLRVGNLERSIEFYTKVLGMKLLRQSENSEYQYTLAFVGYDEESTGSAVIELTYNWGTECYDHGNAFGHIAIG
EEDIYARCDAIAAAGGKVIRPAGPVAGGSTEIAFVEDPDGYKIEFIQMSSAEKGLG

Sequences:

>Translated_136_residues
MAQLLHTMLRVGNLERSIEFYTKVLGMKLLRQSENSEYQYTLAFVGYDEESTGSAVIELTYNWGTECYDHGNAFGHIAIG
EEDIYARCDAIAAAGGKVIRPAGPVAGGSTEIAFVEDPDGYKIEFIQMSSAEKGLG
>Mature_135_residues
AQLLHTMLRVGNLERSIEFYTKVLGMKLLRQSENSEYQYTLAFVGYDEESTGSAVIELTYNWGTECYDHGNAFGHIAIGE
EDIYARCDAIAAAGGKVIRPAGPVAGGSTEIAFVEDPDGYKIEFIQMSSAEKGLG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=34.6938775510204, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=64.9253731343284, Blast_Score=175, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323639, Length=136, Percent_Identity=44.1176470588235, Blast_Score=101, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=35.5704697986577, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=32.6086956521739, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14838; Mature: 14707

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLLHTMLRVGNLERSIEFYTKVLGMKLLRQSENSEYQYTLAFVGYDEESTGSAVIELT
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTECYDHGNAFGHIAIGEEDIYARCDAIAAAGGKVIRPAGPVAGGSTEIAFVEDPDG
ECCCCCEEECCCCEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMSSAEKGLG
CEEEEEEECCCCCCCC
>Mature Secondary Structure 
AQLLHTMLRVGNLERSIEFYTKVLGMKLLRQSENSEYQYTLAFVGYDEESTGSAVIELT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTECYDHGNAFGHIAIGEEDIYARCDAIAAAGGKVIRPAGPVAGGSTEIAFVEDPDG
ECCCCCEEECCCCEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMSSAEKGLG
CEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]