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The map label for this gene is slt [H]

Identifier: 167623792

GI number: 167623792

Start: 2233961

End: 2235850

Strand: Reverse

Name: slt [H]

Synonym: Shal_1862

Alternate gene names: 167623792

Gene position: 2235850-2233961 (Counterclockwise)

Preceding gene: 167623793

Following gene: 167623791

Centisome position: 42.78

GC content: 43.97

Gene sequence:

>1890_bases
ATGCTTGCATGCTCCTCAGTCCATGCGTCTAAATTAACCGAAGAGCAGCAACTTTATTTAGACGCAAGGAGTGCGCTAAA
TAAGCAACAATTAAGCCAATATCAGCCGATGCGAGATAAACTAGATGACTACCCTTTAGCCATCTATTTAGACTTTCACG
ACAAGATCAACGCTATCCTCAAGCTTCCCGCAAAACAGGCAATGAAACAGTTAACCGCCTTTGAGGGTAGCCCATTGTAT
AACACTGGGCGCTACCGTTACCTTAAACGCACAGGTAGTCAAAAACGTTGGAAAGACTTTCTCATCGTCAGCCCCAAAAG
CCCTAACAACATTACCCTGCAATGTTATTTCTACCGTGCCCAACTACAACAGGGTGATAAGCAGGTCGCCTATGACGGTG
CTAAAACTCTTTGGTTATATGGCCGTTCTCGCCCTAAAGAATGCGATCCCTTATTTAAAGAGTGGCAAAAAGCTGGGCTG
CGAAGCCAAGAACTCATCTGGTCACGTATGTTGCTCAGCTTCGAGCAAGGACAGTATGGCCTACTGTCCTATCTTTCACG
CCAATTGACCACTCACAAAAGTGACGCTAAGCGTTTGCTTGCTGTCTATAAAGATCCACGTAGCCTGCGTCATAAATCAA
AATTTAGCGGCAAAGCCAAGATTAATGCAACGATTGTGGATTTAGGCCTAAGAAAATTAGCCAAGAAAGACCTAAAACAA
GCCGTGAAACTGTTTGCCGCTTATCAGAAGGCCGACAGGTTCAGTGATTATCAAGGGAGAAAGCTTAGCCGTTACTTGGT
CAGAAGAGCACTAATTTATCAAACGATTGAACTTCAAAGCTACGTCGATACCATTTTGCCTTTGCTCGACAGCGACGATT
TAGTCGAGATGCGTTTGCGTTGGGCGCTTAGAGATAATGACCAAAAAAGTTTTGCTAAATTCCTACCGCAACTTAGTGAG
CAAAAACAAAATACTGCCCGCTGGAAATATTGGCGTGCAACTCTGCTTGCAAAAGAAAATAGCAAACAAAGCCAAGAGCA
ATCTATGGCACTGTTCGAGACGCTGCGCCTAGAACGCAATTTTTATGGTTACCTTGCAGCGGCTAAGCACAAACAACCTT
ATCAATTGCAAGACAAGCAAACTCAGAGCAACGATAAGCTTAAACAGAAGATATTTAACGACAAAGGCTTAGCACGGGTA
CAAGAGCTAATAGGTATTGATAAGCAGTCTGATGCAAGAGCCGAATGGGTCATGTTACTGAACCGTCATGATAAAGTGAT
GCAAAAAGAATATGCAATCTATGCTGTTGAGCAAAAGTGGCATAGCCTAGGTGTTCAAGCTAGCATACAAGCCAAACTTT
GGAATGATATGCAGATGCGTTTCCCCTATGCCGCGCAAACCTCTTTTACTCATGCCAGCAAAAAGTTCGCGGTGGATATT
GATGAAATTCGCTCGATTTCTCGAAGAGAAAGTGCCTATTACCCCAATGCCACATCTGGAGTGGGGGCACGGGGTTATAT
GCAGTTAATGCCGGCAACCGCTAAGCAAACAGCCAAGAAGACCAAACTCCCCTATCGAGGGACTAAGAGCCTATATGATG
AAAAACTCAATATCTCTCTAGGCAGCGCCTATTACGGTGGTTTACTCAATCAATTTGATCAAAACCGTGTATTAGCTACC
GCATCTTACAATGCTGGCCCTCACCGTATAAATCGCTGGCTTGAAAAGAGTGATGGCAAACTTGATGTTGTCAGTTTTAT
CGAGTCCATCCCCTACCGAGAGACTCGTGAATACGTGCAAGCAGTACTCAGCTATCGAGTGATCTATCAAATTAAGCAGG
GCAAACAAGCTGAGTTATTGTCAGAGCAGGAGCTTAAATTTCATTATTAA

Upstream 100 bases:

>100_bases
AGTCACAAAGTTGATACCACTTGGCACATAATAGGGATAGTTAAGAGGATTCTTGCAAATGCACAAGCAGTTACGTCGCA
TTTTAACCGGGATAATAGCA

Downstream 100 bases:

>100_bases
TACTGAGAGGAGCGTGCAGTTAGCTATTCGAAAGGCTTAGTCATAACGCTATTATTTTCACAACAGATTAATATTAAAGC
AAAAAATCTCGAGTGTAGTC

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MLACSSVHASKLTEEQQLYLDARSALNKQQLSQYQPMRDKLDDYPLAIYLDFHDKINAILKLPAKQAMKQLTAFEGSPLY
NTGRYRYLKRTGSQKRWKDFLIVSPKSPNNITLQCYFYRAQLQQGDKQVAYDGAKTLWLYGRSRPKECDPLFKEWQKAGL
RSQELIWSRMLLSFEQGQYGLLSYLSRQLTTHKSDAKRLLAVYKDPRSLRHKSKFSGKAKINATIVDLGLRKLAKKDLKQ
AVKLFAAYQKADRFSDYQGRKLSRYLVRRALIYQTIELQSYVDTILPLLDSDDLVEMRLRWALRDNDQKSFAKFLPQLSE
QKQNTARWKYWRATLLAKENSKQSQEQSMALFETLRLERNFYGYLAAAKHKQPYQLQDKQTQSNDKLKQKIFNDKGLARV
QELIGIDKQSDARAEWVMLLNRHDKVMQKEYAIYAVEQKWHSLGVQASIQAKLWNDMQMRFPYAAQTSFTHASKKFAVDI
DEIRSISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKTKLPYRGTKSLYDEKLNISLGSAYYGGLLNQFDQNRVLAT
ASYNAGPHRINRWLEKSDGKLDVVSFIESIPYRETREYVQAVLSYRVIYQIKQGKQAELLSEQELKFHY

Sequences:

>Translated_629_residues
MLACSSVHASKLTEEQQLYLDARSALNKQQLSQYQPMRDKLDDYPLAIYLDFHDKINAILKLPAKQAMKQLTAFEGSPLY
NTGRYRYLKRTGSQKRWKDFLIVSPKSPNNITLQCYFYRAQLQQGDKQVAYDGAKTLWLYGRSRPKECDPLFKEWQKAGL
RSQELIWSRMLLSFEQGQYGLLSYLSRQLTTHKSDAKRLLAVYKDPRSLRHKSKFSGKAKINATIVDLGLRKLAKKDLKQ
AVKLFAAYQKADRFSDYQGRKLSRYLVRRALIYQTIELQSYVDTILPLLDSDDLVEMRLRWALRDNDQKSFAKFLPQLSE
QKQNTARWKYWRATLLAKENSKQSQEQSMALFETLRLERNFYGYLAAAKHKQPYQLQDKQTQSNDKLKQKIFNDKGLARV
QELIGIDKQSDARAEWVMLLNRHDKVMQKEYAIYAVEQKWHSLGVQASIQAKLWNDMQMRFPYAAQTSFTHASKKFAVDI
DEIRSISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKTKLPYRGTKSLYDEKLNISLGSAYYGGLLNQFDQNRVLAT
ASYNAGPHRINRWLEKSDGKLDVVSFIESIPYRETREYVQAVLSYRVIYQIKQGKQAELLSEQELKFHY
>Mature_629_residues
MLACSSVHASKLTEEQQLYLDARSALNKQQLSQYQPMRDKLDDYPLAIYLDFHDKINAILKLPAKQAMKQLTAFEGSPLY
NTGRYRYLKRTGSQKRWKDFLIVSPKSPNNITLQCYFYRAQLQQGDKQVAYDGAKTLWLYGRSRPKECDPLFKEWQKAGL
RSQELIWSRMLLSFEQGQYGLLSYLSRQLTTHKSDAKRLLAVYKDPRSLRHKSKFSGKAKINATIVDLGLRKLAKKDLKQ
AVKLFAAYQKADRFSDYQGRKLSRYLVRRALIYQTIELQSYVDTILPLLDSDDLVEMRLRWALRDNDQKSFAKFLPQLSE
QKQNTARWKYWRATLLAKENSKQSQEQSMALFETLRLERNFYGYLAAAKHKQPYQLQDKQTQSNDKLKQKIFNDKGLARV
QELIGIDKQSDARAEWVMLLNRHDKVMQKEYAIYAVEQKWHSLGVQASIQAKLWNDMQMRFPYAAQTSFTHASKKFAVDI
DEIRSISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKTKLPYRGTKSLYDEKLNISLGSAYYGGLLNQFDQNRVLAT
ASYNAGPHRINRWLEKSDGKLDVVSFIESIPYRETREYVQAVLSYRVIYQIKQGKQAELLSEQELKFHY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=634, Percent_Identity=29.8107255520505, Blast_Score=311, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73279; Mature: 73279

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLACSSVHASKLTEEQQLYLDARSALNKQQLSQYQPMRDKLDDYPLAIYLDFHDKINAIL
CCCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHCCCHHHCCCCCCEEEEEECCHHHHHHH
KLPAKQAMKQLTAFEGSPLYNTGRYRYLKRTGSQKRWKDFLIVSPKSPNNITLQCYFYRA
HCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEEEH
QLQQGDKQVAYDGAKTLWLYGRSRPKECDPLFKEWQKAGLRSQELIWSRMLLSFEQGQYG
HHHCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHH
LLSYLSRQLTTHKSDAKRLLAVYKDPRSLRHKSKFSGKAKINATIVDLGLRKLAKKDLKQ
HHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
AVKLFAAYQKADRFSDYQGRKLSRYLVRRALIYQTIELQSYVDTILPLLDSDDLVEMRLR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
WALRDNDQKSFAKFLPQLSEQKQNTARWKYWRATLLAKENSKQSQEQSMALFETLRLERN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FYGYLAAAKHKQPYQLQDKQTQSNDKLKQKIFNDKGLARVQELIGIDKQSDARAEWVMLL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHH
NRHDKVMQKEYAIYAVEQKWHSLGVQASIQAKLWNDMQMRFPYAAQTSFTHASKKFAVDI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHCCCCHHCCCHHHCCCCEEEEH
DEIRSISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKTKLPYRGTKSLYDEKLNISL
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCCHHHHHCCCCEEE
GSAYYGGLLNQFDQNRVLATASYNAGPHRINRWLEKSDGKLDVVSFIESIPYRETREYVQ
CCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
AVLSYRVIYQIKQGKQAELLSEQELKFHY
HHHHHHHHHHHHCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MLACSSVHASKLTEEQQLYLDARSALNKQQLSQYQPMRDKLDDYPLAIYLDFHDKINAIL
CCCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHCCCHHHCCCCCCEEEEEECCHHHHHHH
KLPAKQAMKQLTAFEGSPLYNTGRYRYLKRTGSQKRWKDFLIVSPKSPNNITLQCYFYRA
HCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEEEH
QLQQGDKQVAYDGAKTLWLYGRSRPKECDPLFKEWQKAGLRSQELIWSRMLLSFEQGQYG
HHHCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHH
LLSYLSRQLTTHKSDAKRLLAVYKDPRSLRHKSKFSGKAKINATIVDLGLRKLAKKDLKQ
HHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
AVKLFAAYQKADRFSDYQGRKLSRYLVRRALIYQTIELQSYVDTILPLLDSDDLVEMRLR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
WALRDNDQKSFAKFLPQLSEQKQNTARWKYWRATLLAKENSKQSQEQSMALFETLRLERN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FYGYLAAAKHKQPYQLQDKQTQSNDKLKQKIFNDKGLARVQELIGIDKQSDARAEWVMLL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHH
NRHDKVMQKEYAIYAVEQKWHSLGVQASIQAKLWNDMQMRFPYAAQTSFTHASKKFAVDI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHCCCCHHCCCHHHCCCCEEEEH
DEIRSISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKTKLPYRGTKSLYDEKLNISL
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCCHHHHHCCCCEEE
GSAYYGGLLNQFDQNRVLATASYNAGPHRINRWLEKSDGKLDVVSFIESIPYRETREYVQ
CCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
AVLSYRVIYQIKQGKQAELLSEQELKFHY
HHHHHHHHHHHHCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]