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The map label for this gene is dusC [H]

Identifier: 167623514

GI number: 167623514

Start: 1901250

End: 1902233

Strand: Reverse

Name: dusC [H]

Synonym: Shal_1583

Alternate gene names: 167623514

Gene position: 1902233-1901250 (Counterclockwise)

Preceding gene: 167623523

Following gene: 167623513

Centisome position: 36.39

GC content: 46.04

Gene sequence:

>984_bases
ATGCAGCGCCTTAAAAATACCGATATTTTGGACAGTTCTGTGCGAATAATTTTGGCGCCGATGGAAGGCGTTGTAGATGA
TTTAATGCGAGAGATCCTCTCTGCAATCAATCCTTATGATCTTATGGTTACGGAGTTCGTCCGTGTCGTAGACCAACTGC
TACCTGAAAAGGTCTTTTATCGTCTTTGCCCAGAGTTGCAAACTGGTGGTCAAACACTATCCGGTACACCTGTGCGAGTG
CAGCTACTGGGACAAAATCCCGATTGGATGGCAGAAAACGCAGCAAGGGCTATTGAACTGGGATCAAAAGGTGTCGATGT
GAACTTTGGCTGCCCTGCTAAAATGGTCAATCGCAGCAATGGTGGCGCCGTGATGTTGCAATATCCGGAGCAACTCTACA
ATGTTGTGAAAGCGATGCGTGATGCAGTTCCTAGCGACCAGCCCGTTACCGCCAAAATACGCTTAGGCTTTAATGATAAG
TCACTCTATATGGAAAATGCTCTCGCTATTTATGAGGCTGGCGCCAGTGAATTAGCTGTACACGCTCGCAGTAAAGTTGA
TGGCTATAAGCCACCTGCCTACTGGGAATATATCACCGACATTAATGCTAAGTTACCGATCCCAGTTATTGCTAACGGCG
AGATCTGGAACCGTGATGATGCCGAGCGCTGTATGAGTATTACTGGCTGTGACAGTGTGATGGTGGGACGCGGAGCAATA
TCGCTACCGAACTTGGCTGCGAACATTAAAGGCGATACTGCTTATACTTGGCAGCAATCTCTTGATCTCATGCTTGAGTA
CACGAATAGAGAGTTAGAAGGTAGAAAGAGTACCTATTATCCCGCTCGAATTAAGCAATGGTTTGGCTATCTGAATCGGC
AATATCCAGAGGCTGATACCCTCTTTAGAGAGCTTAGGGTATTTAAAACAGCAGATGAGATCATTGGTGTATTAAAACGC
GCACAGGACAATTTGGCGAATTAA

Upstream 100 bases:

>100_bases
ATCTTCTACAAACAAAGCATTAACCTTTTGAGAAAGCATTCCCTAGGCGAATCGATAAAAGTATCAAGTCTATTTGGCCG
AGGGTTTAGGATGAGGTAGA

Downstream 100 bases:

>100_bases
TTAGTATTTAATTGAGCTAAATCCGCAAAATATAGCACTGAATTGATCTTAGTCATAACGCATCACGCCCGCATGTTTAG
ACTAAAAAGCCGTAGAGTTA

Product: dihydrouridine synthase DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQRLKNTDILDSSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFYRLCPELQTGGQTLSGTPVRV
QLLGQNPDWMAENAARAIELGSKGVDVNFGCPAKMVNRSNGGAVMLQYPEQLYNVVKAMRDAVPSDQPVTAKIRLGFNDK
SLYMENALAIYEAGASELAVHARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSITGCDSVMVGRGAI
SLPNLAANIKGDTAYTWQQSLDLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYPEADTLFRELRVFKTADEIIGVLKR
AQDNLAN

Sequences:

>Translated_327_residues
MQRLKNTDILDSSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFYRLCPELQTGGQTLSGTPVRV
QLLGQNPDWMAENAARAIELGSKGVDVNFGCPAKMVNRSNGGAVMLQYPEQLYNVVKAMRDAVPSDQPVTAKIRLGFNDK
SLYMENALAIYEAGASELAVHARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSITGCDSVMVGRGAI
SLPNLAANIKGDTAYTWQQSLDLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYPEADTLFRELRVFKTADEIIGVLKR
AQDNLAN
>Mature_327_residues
MQRLKNTDILDSSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFYRLCPELQTGGQTLSGTPVRV
QLLGQNPDWMAENAARAIELGSKGVDVNFGCPAKMVNRSNGGAVMLQYPEQLYNVVKAMRDAVPSDQPVTAKIRLGFNDK
SLYMENALAIYEAGASELAVHARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSITGCDSVMVGRGAI
SLPNLAANIKGDTAYTWQQSLDLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYPEADTLFRELRVFKTADEIIGVLKR
AQDNLAN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=229, Percent_Identity=31.0043668122271, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=30.5676855895196, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI31742496, Length=173, Percent_Identity=31.7919075144509, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI8923374, Length=257, Percent_Identity=28.7937743190661, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40807366, Length=269, Percent_Identity=28.6245353159851, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788462, Length=303, Percent_Identity=53.7953795379538, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1789660, Length=231, Percent_Identity=32.9004329004329, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI145693211, Length=296, Percent_Identity=26.6891891891892, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=27.2321428571429, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=201, Percent_Identity=31.8407960199005, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=257, Percent_Identity=29.5719844357977, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=195, Percent_Identity=24.6153846153846, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323437, Length=158, Percent_Identity=29.746835443038, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=32.3404255319149, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24580595, Length=192, Percent_Identity=30.7291666666667, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=192, Percent_Identity=30.7291666666667, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45549423, Length=156, Percent_Identity=29.4871794871795, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921524, Length=176, Percent_Identity=29.5454545454545, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36667; Mature: 36667

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLKNTDILDSSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFY
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RLCPELQTGGQTLSGTPVRVQLLGQNPDWMAENAARAIELGSKGVDVNFGCPAKMVNRSN
HHCCHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCHHHHCCCC
GGAVMLQYPEQLYNVVKAMRDAVPSDQPVTAKIRLGFNDKSLYMENALAIYEAGASELAV
CCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHH
HARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSITGCDSVMVGRGAI
HHHHHCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCC
SLPNLAANIKGDTAYTWQQSLDLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYPEADT
CCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
LFRELRVFKTADEIIGVLKRAQDNLAN
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRLKNTDILDSSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFY
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RLCPELQTGGQTLSGTPVRVQLLGQNPDWMAENAARAIELGSKGVDVNFGCPAKMVNRSN
HHCCHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCHHHHCCCC
GGAVMLQYPEQLYNVVKAMRDAVPSDQPVTAKIRLGFNDKSLYMENALAIYEAGASELAV
CCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHH
HARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSITGCDSVMVGRGAI
HHHHHCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCC
SLPNLAANIKGDTAYTWQQSLDLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYPEADT
CCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
LFRELRVFKTADEIIGVLKRAQDNLAN
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]