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The map label for this gene is fixJ [H]

Identifier: 167622347

GI number: 167622347

Start: 476840

End: 477514

Strand: Reverse

Name: fixJ [H]

Synonym: Shal_0407

Alternate gene names: 167622347

Gene position: 477514-476840 (Counterclockwise)

Preceding gene: 167622348

Following gene: 167622346

Centisome position: 9.14

GC content: 45.63

Gene sequence:

>675_bases
ATGATAATGACAAACACTAACCTTTATCTAGTTGACGACGATGAGTCTGTATTAGACGCACTAGACTTTGCCCTGCAGGG
AGAGGGCTATCAGCCTCTGTCATTTAACTCTGCCGAAGCTTTTTGGCTGTGGGCAAAACAGCATGCCGATAAAATGAACG
AAGGCGGTTGCTTGATCTTAGACTCTCGGATGCCAGGTATGAGCGGTCAGGAACTTCAGTACCAGCTTAGCCAAACAGAC
TGTTCACTAGGGATTGTATTTCTTACCGGCCATGGAGATGTCCCTATGGCGGTTGATGCCCTTAAGCTTGGAGCCGTAGA
CTTTCTGCAAAAACCGATTGAAATTACCAAGCTTCTTAAGGCCGTATCACAGGCGTTCGATGCATCTACATCCTCCAAAG
AGTTGAAATCTGCCATTCGCTGCTATGAAGAGTTAACGCTACGTGAGCGAGATATACTGCAACTACTCGCCCAGGGTAAA
ACAAATCAGCAGCTGGCCGAGCTGTTATTTATCTCAGCGCGAACTGTTGAGGTACACAGATCAAATATGTTGAAGCATTT
ACAGATGGACTCACTTGCTAAGATGGTACGCCTCTATACTCTAGTAGAGCCGTACTTAAGCAGTATAGCCCCACCTCCTT
TACGAATTCGTCGTAAAACAAAGCACAATGATTAA

Upstream 100 bases:

>100_bases
AAAGAGGTGGCTGAATCTCATCTTGGGCACCTATCATTTAACAATCTAGATAATGGCGGCTGCTGCGTGAGCATCGAGTT
TCCAATACCAAATATAAGAG

Downstream 100 bases:

>100_bases
TTTATTGCTCTGTGCCCTGCATCACTCAAGTTCACAGCAAAATATAGAGAGCAGCTCACAACCACACTAACTATGTGGAA
ATCCCTTATAGAAGCCTATC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MIMTNTNLYLVDDDESVLDALDFALQGEGYQPLSFNSAEAFWLWAKQHADKMNEGGCLILDSRMPGMSGQELQYQLSQTD
CSLGIVFLTGHGDVPMAVDALKLGAVDFLQKPIEITKLLKAVSQAFDASTSSKELKSAIRCYEELTLRERDILQLLAQGK
TNQQLAELLFISARTVEVHRSNMLKHLQMDSLAKMVRLYTLVEPYLSSIAPPPLRIRRKTKHND

Sequences:

>Translated_224_residues
MIMTNTNLYLVDDDESVLDALDFALQGEGYQPLSFNSAEAFWLWAKQHADKMNEGGCLILDSRMPGMSGQELQYQLSQTD
CSLGIVFLTGHGDVPMAVDALKLGAVDFLQKPIEITKLLKAVSQAFDASTSSKELKSAIRCYEELTLRERDILQLLAQGK
TNQQLAELLFISARTVEVHRSNMLKHLQMDSLAKMVRLYTLVEPYLSSIAPPPLRIRRKTKHND
>Mature_224_residues
MIMTNTNLYLVDDDESVLDALDFALQGEGYQPLSFNSAEAFWLWAKQHADKMNEGGCLILDSRMPGMSGQELQYQLSQTD
CSLGIVFLTGHGDVPMAVDALKLGAVDFLQKPIEITKLLKAVSQAFDASTSSKELKSAIRCYEELTLRERDILQLLAQGK
TNQQLAELLFISARTVEVHRSNMLKHLQMDSLAKMVRLYTLVEPYLSSIAPPPLRIRRKTKHND

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=125, Percent_Identity=35.2, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788905, Length=155, Percent_Identity=30.9677419354839, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787473, Length=129, Percent_Identity=37.2093023255814, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25147; Mature: 25147

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMTNTNLYLVDDDESVLDALDFALQGEGYQPLSFNSAEAFWLWAKQHADKMNEGGCLIL
CEEECCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEE
DSRMPGMSGQELQYQLSQTDCSLGIVFLTGHGDVPMAVDALKLGAVDFLQKPIEITKLLK
CCCCCCCCCHHHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHH
AVSQAFDASTSSKELKSAIRCYEELTLRERDILQLLAQGKTNQQLAELLFISARTVEVHR
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SNMLKHLQMDSLAKMVRLYTLVEPYLSSIAPPPLRIRRKTKHND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MIMTNTNLYLVDDDESVLDALDFALQGEGYQPLSFNSAEAFWLWAKQHADKMNEGGCLIL
CEEECCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEE
DSRMPGMSGQELQYQLSQTDCSLGIVFLTGHGDVPMAVDALKLGAVDFLQKPIEITKLLK
CCCCCCCCCHHHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHH
AVSQAFDASTSSKELKSAIRCYEELTLRERDILQLLAQGKTNQQLAELLFISARTVEVHR
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SNMLKHLQMDSLAKMVRLYTLVEPYLSSIAPPPLRIRRKTKHND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]