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The map label for this gene is fixL [H]

Identifier: 167622348

GI number: 167622348

Start: 477495

End: 479276

Strand: Reverse

Name: fixL [H]

Synonym: Shal_0408

Alternate gene names: 167622348

Gene position: 479276-477495 (Counterclockwise)

Preceding gene: 167622349

Following gene: 167622347

Centisome position: 9.17

GC content: 45.62

Gene sequence:

>1782_bases
ATGCGATTTTTATACTTAGCCAGCCTTCTTCTCTGTTTACTTGTGCCAATAAAAACGCTGCAAAGCCAGCCCATCGCTCA
AACGCAGACAGCTAAAACCGATCAGATCGTGGCTGTGGGGGTATTTGCGGATCGGGGACTAGATGAAGCTGTGCAACGCT
GGCAGCCGACCATAGATTGGTTAAGTCAACATATTGAAGGGTATCAGTTTAAGTTAATCCCTCTGTCACTTTCTCAACTT
CAAAATGAGCTGAGTCAGCAAAAGCTACAATTCATTATCATCAACCCATCTGAGAGTATCCGCATTGCTCGTAGACAGTC
ACTTTCCTGGTTAGCAACCCTATTAAGCCCCCTTAATGGTGGGACCTCAATTGCTACAGGTTCAGTAATATGGGTCAAGT
CTGACAGCCCTTACCAAAATTTACATCAACTCGCAGGACAAAAAATTGGTACCGCCAGTCCTCAGGCCTTTGGCGGCTAT
ATGGCAATGCAGCGGGAGCTTCAACGACTTGGGCGCGACAGTGACCTTCTAATCAACACAAAAGTCATTGGTTACCCCCA
TGAATCTGTGGTTGCAGAGCTGCAAGAAGGTGATATCGCAGCGGCTATTTTACCGGTTTGCTTAGTTGAATCTATGATAC
GTAAGGGGCAAATCTCTCCCAATGAACTTAGACCAATTAATGATATGACTCCTTCAGGCTTCGAGTGCCGAGTCTCTACT
CGTTTATATCCAAACTGGGGCTTTGCCATGACAGGTAGTGCCGATCGCACCTTAGCACGAGAGATCCTGCAATCTTTACT
CGCGATCCCAAAAAACTCAAATACGGCACAAGCGGCGCAATCCCTTGGTTGGACCGTACCAGAGAGTCAGATTGAGCTGG
ATAATCTATTTAATGATCTGGGAGTTCACCCTCTACAGCAGCCCTGGTGGCAACTTTTGTGGAGCTGGGTACAAACCCAC
TATTACCTGATTATCTTGATGCTTATTGTGGCGACATTTTTACCTATCCAGCATCTAATCTTAACGCTTCGCTATCGCCG
TAACAGTCGAAAACTCGGCTTGGCCCAAGCAAACCTGCAACAGGCACAACGACGTGCATTAGTCGATAAAATGGGTTCCA
GCTTAGCCCATGAACTCAACCAGCCTTTGTCAGCAATTCGCCTCTATACTGAAGGTGAGCTATCTCGTCGTCAAAAAGGC
AGTAGCCAAACTGATATTGATCAGCTGCTCGGAAAAATTCGCCGTCAGGTTATTCGAGTCGATGAGGTCGTTAGCCGCTT
CCGCCAGCTACTACATAAGCAAGCAGTACAAAAATCTCAGCTAGATATTGCATTACTCCTGCGTGATACCGTCAGTTTAG
TTGAGCTTTATGCACATACCCACAAGGTTGAAATAACCTTTCACCCTACTCATGATGAAATGATGTTTTTTGGTGATAGA
GCAGGATTAGAGCAGCTACTGGTCAACTTACTGACTAATGCGATAGATGCATCGGCATCATCGAGTAAAAAGCAGGTATG
GGTCACTTTAACCTTAGCCAGTAGCCGTATTAAAATAAGCATACAAGATACTGGACAGGGGCTCGAGAGGCCCATAGCTG
AGTTGCTAACCCCTTTTGTTACCACTAAAAAAGAGGGCGTGGGACTCGGATTAAGCATCTGTAAAGAGGTGGCTGAATCT
CATCTTGGGCACCTATCATTTAACAATCTAGATAATGGCGGCTGCTGCGTGAGCATCGAGTTTCCAATACCAAATATAAG
AGATGATAATGACAAACACTAA

Upstream 100 bases:

>100_bases
ACAAAACTCACTAAGGGGTCACTGCAATCGTTATCGACTTTAGTAATACTAACGACTCTCTATACTTAGCTAATACGAAC
TCTGCTTAGGGTGCTCTGTA

Downstream 100 bases:

>100_bases
CCTTTATCTAGTTGACGACGATGAGTCTGTATTAGACGCACTAGACTTTGCCCTGCAGGGAGAGGGCTATCAGCCTCTGT
CATTTAACTCTGCCGAAGCT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MRFLYLASLLLCLLVPIKTLQSQPIAQTQTAKTDQIVAVGVFADRGLDEAVQRWQPTIDWLSQHIEGYQFKLIPLSLSQL
QNELSQQKLQFIIINPSESIRIARRQSLSWLATLLSPLNGGTSIATGSVIWVKSDSPYQNLHQLAGQKIGTASPQAFGGY
MAMQRELQRLGRDSDLLINTKVIGYPHESVVAELQEGDIAAAILPVCLVESMIRKGQISPNELRPINDMTPSGFECRVST
RLYPNWGFAMTGSADRTLAREILQSLLAIPKNSNTAQAAQSLGWTVPESQIELDNLFNDLGVHPLQQPWWQLLWSWVQTH
YYLIILMLIVATFLPIQHLILTLRYRRNSRKLGLAQANLQQAQRRALVDKMGSSLAHELNQPLSAIRLYTEGELSRRQKG
SSQTDIDQLLGKIRRQVIRVDEVVSRFRQLLHKQAVQKSQLDIALLLRDTVSLVELYAHTHKVEITFHPTHDEMMFFGDR
AGLEQLLVNLLTNAIDASASSSKKQVWVTLTLASSRIKISIQDTGQGLERPIAELLTPFVTTKKEGVGLGLSICKEVAES
HLGHLSFNNLDNGGCCVSIEFPIPNIRDDNDKH

Sequences:

>Translated_593_residues
MRFLYLASLLLCLLVPIKTLQSQPIAQTQTAKTDQIVAVGVFADRGLDEAVQRWQPTIDWLSQHIEGYQFKLIPLSLSQL
QNELSQQKLQFIIINPSESIRIARRQSLSWLATLLSPLNGGTSIATGSVIWVKSDSPYQNLHQLAGQKIGTASPQAFGGY
MAMQRELQRLGRDSDLLINTKVIGYPHESVVAELQEGDIAAAILPVCLVESMIRKGQISPNELRPINDMTPSGFECRVST
RLYPNWGFAMTGSADRTLAREILQSLLAIPKNSNTAQAAQSLGWTVPESQIELDNLFNDLGVHPLQQPWWQLLWSWVQTH
YYLIILMLIVATFLPIQHLILTLRYRRNSRKLGLAQANLQQAQRRALVDKMGSSLAHELNQPLSAIRLYTEGELSRRQKG
SSQTDIDQLLGKIRRQVIRVDEVVSRFRQLLHKQAVQKSQLDIALLLRDTVSLVELYAHTHKVEITFHPTHDEMMFFGDR
AGLEQLLVNLLTNAIDASASSSKKQVWVTLTLASSRIKISIQDTGQGLERPIAELLTPFVTTKKEGVGLGLSICKEVAES
HLGHLSFNNLDNGGCCVSIEFPIPNIRDDNDKH
>Mature_593_residues
MRFLYLASLLLCLLVPIKTLQSQPIAQTQTAKTDQIVAVGVFADRGLDEAVQRWQPTIDWLSQHIEGYQFKLIPLSLSQL
QNELSQQKLQFIIINPSESIRIARRQSLSWLATLLSPLNGGTSIATGSVIWVKSDSPYQNLHQLAGQKIGTASPQAFGGY
MAMQRELQRLGRDSDLLINTKVIGYPHESVVAELQEGDIAAAILPVCLVESMIRKGQISPNELRPINDMTPSGFECRVST
RLYPNWGFAMTGSADRTLAREILQSLLAIPKNSNTAQAAQSLGWTVPESQIELDNLFNDLGVHPLQQPWWQLLWSWVQTH
YYLIILMLIVATFLPIQHLILTLRYRRNSRKLGLAQANLQQAQRRALVDKMGSSLAHELNQPLSAIRLYTEGELSRRQKG
SSQTDIDQLLGKIRRQVIRVDEVVSRFRQLLHKQAVQKSQLDIALLLRDTVSLVELYAHTHKVEITFHPTHDEMMFFGDR
AGLEQLLVNLLTNAIDASASSSKKQVWVTLTLASSRIKISIQDTGQGLERPIAELLTPFVTTKKEGVGLGLSICKEVAES
HLGHLSFNNLDNGGCCVSIEFPIPNIRDDNDKH

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=269, Percent_Identity=26.3940520446097, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=26.6375545851528, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=26.8292682926829, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66399; Mature: 66399

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLYLASLLLCLLVPIKTLQSQPIAQTQTAKTDQIVAVGVFADRGLDEAVQRWQPTIDW
CHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEHHHCCHHHHHHHHCCHHHH
LSQHIEGYQFKLIPLSLSQLQNELSQQKLQFIIINPSESIRIARRQSLSWLATLLSPLNG
HHHHHCCCEEEEEECCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
GTSIATGSVIWVKSDSPYQNLHQLAGQKIGTASPQAFGGYMAMQRELQRLGRDSDLLINT
CCCEECCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KVIGYPHESVVAELQEGDIAAAILPVCLVESMIRKGQISPNELRPINDMTPSGFECRVST
EEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEEE
RLYPNWGFAMTGSADRTLAREILQSLLAIPKNSNTAQAAQSLGWTVPESQIELDNLFNDL
EECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
GVHPLQQPWWQLLWSWVQTHYYLIILMLIVATFLPIQHLILTLRYRRNSRKLGLAQANLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
QAQRRALVDKMGSSLAHELNQPLSAIRLYTEGELSRRQKGSSQTDIDQLLGKIRRQVIRV
HHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DEVVSRFRQLLHKQAVQKSQLDIALLLRDTVSLVELYAHTHKVEITFHPTHDEMMFFGDR
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCEEECCC
AGLEQLLVNLLTNAIDASASSSKKQVWVTLTLASSRIKISIQDTGQGLERPIAELLTPFV
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHH
TTKKEGVGLGLSICKEVAESHLGHLSFNNLDNGGCCVSIEFPIPNIRDDNDKH
HCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRFLYLASLLLCLLVPIKTLQSQPIAQTQTAKTDQIVAVGVFADRGLDEAVQRWQPTIDW
CHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEHHHCCHHHHHHHHCCHHHH
LSQHIEGYQFKLIPLSLSQLQNELSQQKLQFIIINPSESIRIARRQSLSWLATLLSPLNG
HHHHHCCCEEEEEECCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
GTSIATGSVIWVKSDSPYQNLHQLAGQKIGTASPQAFGGYMAMQRELQRLGRDSDLLINT
CCCEECCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KVIGYPHESVVAELQEGDIAAAILPVCLVESMIRKGQISPNELRPINDMTPSGFECRVST
EEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEEE
RLYPNWGFAMTGSADRTLAREILQSLLAIPKNSNTAQAAQSLGWTVPESQIELDNLFNDL
EECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
GVHPLQQPWWQLLWSWVQTHYYLIILMLIVATFLPIQHLILTLRYRRNSRKLGLAQANLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
QAQRRALVDKMGSSLAHELNQPLSAIRLYTEGELSRRQKGSSQTDIDQLLGKIRRQVIRV
HHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DEVVSRFRQLLHKQAVQKSQLDIALLLRDTVSLVELYAHTHKVEITFHPTHDEMMFFGDR
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCEEECCC
AGLEQLLVNLLTNAIDASASSSKKQVWVTLTLASSRIKISIQDTGQGLERPIAELLTPFV
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHH
TTKKEGVGLGLSICKEVAESHLGHLSFNNLDNGGCCVSIEFPIPNIRDDNDKH
HCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]