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The map label for this gene is kojP [H]

Identifier: 167040825

GI number: 167040825

Start: 2203296

End: 2205623

Strand: Reverse

Name: kojP [H]

Synonym: Teth514_2202

Alternate gene names: 167040825

Gene position: 2205623-2203296 (Counterclockwise)

Preceding gene: 167040826

Following gene: 167040824

Centisome position: 89.76

GC content: 35.05

Gene sequence:

>2328_bases
GTGGTAAAGCACATGTTTTTAGAGGATGTAAACAATTTAATAAGTGATGACAAATGGCTTATTTTCCAAAATGAGTATAA
TACAGAGGTAAATCCTCGATATGAGACCCTTTTTACACTTACAAATGGTTACATGGGCGTAAGAGGTACTTTTGAGGAAG
GAAGCGAGGGAGAAAGGTCGGGAAATTTTATTGCAGGAATTTTTGGCAAGTCAGATGCGCAGGTTAGAGAAATAGTAAAT
GCTCAAAATTGGTTGAGAATAAAGTTGTATGTTGAAGGTGAAGAATTAAGTTTGGATAAATGCCAGTTGATAGAATTTAA
AAGAATTCTTGATATGAAAAAAGGTATTCTATTTAGGAGTATGTTGATAAAAGACAGCAAAGATAGAATTACTCGAATTG
AGGGATACAGGTTTATAAGCCGTAGCGACCTTCATCGTTCTGCAATTAAGCTATTTGTAACACCTGTAAATTACAGTGGT
GTTGTAGGTATAGAGAGCATTATTGATGGGACTGTTTTAAATTCAGCAGATAGCCCAAAGCATAGGGTAAAGCATTTGAA
AGTGGCTGACAATAGCAGTTTAAATAAAAGCGGAGTTTATCTTGAAACAGCAACTATTGACGATGATATTCGCATTGCAA
CAGGTAGTGCAGTGAGGTTATATCATTATGAGGATAAAGAGAAAAATAACATAGCTAAATTTAAGAGATTTTTGCCTTTA
GGTGAAATGAGTATTGAATACTTTGAGTTTGATGGCACAGAGAACAAAACAGTAGTAATTGACAAATTTATTATAACCTA
TACCTCAAGAGACGTAAAAAAAGGCCTATTAAAGAGTACGGTGGAAAAAGAACTCTTTGCTTTTGCTGGAGAAGGTATTG
ACAAAGAGTTGCAGAGACATATTGAGGTATATGAAGAACTATGGTCTGTTGCAGATATAAATATTGAAGGGGATGAAGAA
GCAGATAAAGCTTTGAGGTTTAATATTTTTCATCTCATGAGTTCTGTCAATGAAAATGACCCCATGGTAAGTATCGCTGC
AAAGGCCCTTCATGGTGAGGGATACAAAGGCCATGTCTTTTGGGATACAGAAATATTTATGCTTCCGTTTTTCATATATG
TGCATCCAAAAGCGGCAAAGACACTTTTGATGTACAGGTACAATATGCTAGATGCAGCGAGAAAAAATGCGGCTTTAAAC
GGGTATAAGGGAGCACAATACCCTTGGGAATCTGCAGATACAGGAGAGGAAGAGACACCTAAATGGGGATTTGATTACAT
GGGAAACCCTGTAAGGATATGGACGGGTGATTTAGAGCATCATATAACTGCTGATATAGCTTTTGCAGTGTGGGAGTATT
TTAGAGCGACAGAGGATATTGAGTTTATGTTGAATTACGGTGCAGAAATCATTTTTGAGACTGCAAGATTTTGGGTATCT
AGATGTGAATATGTAAAAGAATTAGACAGGTATGAAATAAACAATGTCATAGGTCCTGATGAATTTCATGAACATGTTGA
TAATAATGCTTATACTAATTACCTTGCAAAATGGAATATTAAAAAGGGACTTGAACTAATCAATATGTTAAAAGAAAAAT
ACCCTGAACATTATCATGCTATATCAAACAAGATATGTTTGACAAATGAGGAAATGGAAAAGTGGAAAGAAGTTGAAGAA
AAAATATATATACCTTATGACAAAGACAAAAAGCTGATAGAACAATTTGAAGGCTATTTTGATAAAAAAGATTATGTTAT
TGATAAATTTGATGAAAACAATATGCCTATATGGCCTGAAGGTGTTGATATAACAAAATTGGGTGATACCCAGCTTATTA
AACAGGCTGATGTTGTTATGTTAATGCTTTTGTTAGGTGAGGAATTTGACGAAGAAACGAAAAGAATCAATTACGAATAT
TATGAAAAGCGAACTATGCACAAATCTTCATTAGGTCCCAGCATGTATGCCATTATGGGGTTAAAAGTAGGGGACCACAA
AAACGCATACCAGTCTTTTATAAGAAGTGCCAACGTGGACCTTGTGGACAATCAGGGGAACACTAAAGAAGGTTTGCATG
CTGCATCTGCTGGTGGTACATGGCAAGTAGCTGTTTTTGGATTTGGCGGAATGGAAATTGACAAAGAAGGGGCATTAAAT
ATAAATTCGTGGCTGCCAGAAAAATGGGATAAACTTTCCTATAAAGTATTCTGGAAAGGTAATTTAATTGAGGTAATTGT
TACAAAAGAGGATGTGTCAGTAAAAAAATTAGAAGGAAAAGGAAATATAAAAGTAAAGGTAAAAGGGAAAGAGATTACGT
TACAATAG

Upstream 100 bases:

>100_bases
AGCTTGTAATTAGAGATTCAGCTAGAAAGATATAATTTTTTTTAAAAAGTACCCAAAACGTTTCGGATAAAATTTTTAGC
AGGAATCAGGAGGTATATAA

Downstream 100 bases:

>100_bases
TCAAAAAGGTACAGGCAGGTGGTAACCTGCTATACCTTAAAATAAATTGATTAAGTTTTAAGGGGGGATAAGTGGAGTTA
GAGTAAAATTTTAAGTTCTC

Product: Kojibiose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MVKHMFLEDVNNLISDDKWLIFQNEYNTEVNPRYETLFTLTNGYMGVRGTFEEGSEGERSGNFIAGIFGKSDAQVREIVN
AQNWLRIKLYVEGEELSLDKCQLIEFKRILDMKKGILFRSMLIKDSKDRITRIEGYRFISRSDLHRSAIKLFVTPVNYSG
VVGIESIIDGTVLNSADSPKHRVKHLKVADNSSLNKSGVYLETATIDDDIRIATGSAVRLYHYEDKEKNNIAKFKRFLPL
GEMSIEYFEFDGTENKTVVIDKFIITYTSRDVKKGLLKSTVEKELFAFAGEGIDKELQRHIEVYEELWSVADINIEGDEE
ADKALRFNIFHLMSSVNENDPMVSIAAKALHGEGYKGHVFWDTEIFMLPFFIYVHPKAAKTLLMYRYNMLDAARKNAALN
GYKGAQYPWESADTGEEETPKWGFDYMGNPVRIWTGDLEHHITADIAFAVWEYFRATEDIEFMLNYGAEIIFETARFWVS
RCEYVKELDRYEINNVIGPDEFHEHVDNNAYTNYLAKWNIKKGLELINMLKEKYPEHYHAISNKICLTNEEMEKWKEVEE
KIYIPYDKDKKLIEQFEGYFDKKDYVIDKFDENNMPIWPEGVDITKLGDTQLIKQADVVMLMLLLGEEFDEETKRINYEY
YEKRTMHKSSLGPSMYAIMGLKVGDHKNAYQSFIRSANVDLVDNQGNTKEGLHAASAGGTWQVAVFGFGGMEIDKEGALN
INSWLPEKWDKLSYKVFWKGNLIEVIVTKEDVSVKKLEGKGNIKVKVKGKEITLQ

Sequences:

>Translated_775_residues
MVKHMFLEDVNNLISDDKWLIFQNEYNTEVNPRYETLFTLTNGYMGVRGTFEEGSEGERSGNFIAGIFGKSDAQVREIVN
AQNWLRIKLYVEGEELSLDKCQLIEFKRILDMKKGILFRSMLIKDSKDRITRIEGYRFISRSDLHRSAIKLFVTPVNYSG
VVGIESIIDGTVLNSADSPKHRVKHLKVADNSSLNKSGVYLETATIDDDIRIATGSAVRLYHYEDKEKNNIAKFKRFLPL
GEMSIEYFEFDGTENKTVVIDKFIITYTSRDVKKGLLKSTVEKELFAFAGEGIDKELQRHIEVYEELWSVADINIEGDEE
ADKALRFNIFHLMSSVNENDPMVSIAAKALHGEGYKGHVFWDTEIFMLPFFIYVHPKAAKTLLMYRYNMLDAARKNAALN
GYKGAQYPWESADTGEEETPKWGFDYMGNPVRIWTGDLEHHITADIAFAVWEYFRATEDIEFMLNYGAEIIFETARFWVS
RCEYVKELDRYEINNVIGPDEFHEHVDNNAYTNYLAKWNIKKGLELINMLKEKYPEHYHAISNKICLTNEEMEKWKEVEE
KIYIPYDKDKKLIEQFEGYFDKKDYVIDKFDENNMPIWPEGVDITKLGDTQLIKQADVVMLMLLLGEEFDEETKRINYEY
YEKRTMHKSSLGPSMYAIMGLKVGDHKNAYQSFIRSANVDLVDNQGNTKEGLHAASAGGTWQVAVFGFGGMEIDKEGALN
INSWLPEKWDKLSYKVFWKGNLIEVIVTKEDVSVKKLEGKGNIKVKVKGKEITLQ
>Mature_775_residues
MVKHMFLEDVNNLISDDKWLIFQNEYNTEVNPRYETLFTLTNGYMGVRGTFEEGSEGERSGNFIAGIFGKSDAQVREIVN
AQNWLRIKLYVEGEELSLDKCQLIEFKRILDMKKGILFRSMLIKDSKDRITRIEGYRFISRSDLHRSAIKLFVTPVNYSG
VVGIESIIDGTVLNSADSPKHRVKHLKVADNSSLNKSGVYLETATIDDDIRIATGSAVRLYHYEDKEKNNIAKFKRFLPL
GEMSIEYFEFDGTENKTVVIDKFIITYTSRDVKKGLLKSTVEKELFAFAGEGIDKELQRHIEVYEELWSVADINIEGDEE
ADKALRFNIFHLMSSVNENDPMVSIAAKALHGEGYKGHVFWDTEIFMLPFFIYVHPKAAKTLLMYRYNMLDAARKNAALN
GYKGAQYPWESADTGEEETPKWGFDYMGNPVRIWTGDLEHHITADIAFAVWEYFRATEDIEFMLNYGAEIIFETARFWVS
RCEYVKELDRYEINNVIGPDEFHEHVDNNAYTNYLAKWNIKKGLELINMLKEKYPEHYHAISNKICLTNEEMEKWKEVEE
KIYIPYDKDKKLIEQFEGYFDKKDYVIDKFDENNMPIWPEGVDITKLGDTQLIKQADVVMLMLLLGEEFDEETKRINYEY
YEKRTMHKSSLGPSMYAIMGLKVGDHKNAYQSFIRSANVDLVDNQGNTKEGLHAASAGGTWQVAVFGFGGMEIDKEGALN
INSWLPEKWDKLSYKVFWKGNLIEVIVTKEDVSVKKLEGKGNIKVKVKGKEITLQ

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=480, Percent_Identity=26.0416666666667, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI1787575, Length=761, Percent_Identity=30.880420499343, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6325283, Length=453, Percent_Identity=25.1655629139073, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24583760, Length=469, Percent_Identity=28.997867803838, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 89568; Mature: 89568

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKHMFLEDVNNLISDDKWLIFQNEYNTEVNPRYETLFTLTNGYMGVRGTFEEGSEGERS
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCC
GNFIAGIFGKSDAQVREIVNAQNWLRIKLYVEGEELSLDKCQLIEFKRILDMKKGILFRS
CCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
MLIKDSKDRITRIEGYRFISRSDLHRSAIKLFVTPVNYSGVVGIESIIDGTVLNSADSPK
HHHCCCHHHHHHCCCEEEECHHHHHHEEEEEEEEECCCCCCEEHHHHHCCCCCCCCCCCH
HRVKHLKVADNSSLNKSGVYLETATIDDDIRIATGSAVRLYHYEDKEKNNIAKFKRFLPL
HHHHEEEECCCCCCCCCCEEEEEEECCCCEEEECCCEEEEEEECCCCCCCHHHHHHHCCC
GEMSIEYFEFDGTENKTVVIDKFIITYTSRDVKKGLLKSTVEKELFAFAGEGIDKELQRH
CCCEEEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEVYEELWSVADINIEGDEEADKALRFNIFHLMSSVNENDPMVSIAAKALHGEGYKGHVF
HHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCEEEEEE
WDTEIFMLPFFIYVHPKAAKTLLMYRYNMLDAARKNAALNGYKGAQYPWESADTGEEETP
ECCHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
KWGFDYMGNPVRIWTGDLEHHITADIAFAVWEYFRATEDIEFMLNYGAEIIFETARFWVS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHH
RCEYVKELDRYEINNVIGPDEFHEHVDNNAYTNYLAKWNIKKGLELINMLKEKYPEHYHA
HHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHEEECCHHHHHHHHHHHHHHCCHHHHH
ISNKICLTNEEMEKWKEVEEKIYIPYDKDKKLIEQFEGYFDKKDYVIDKFDENNMPIWPE
HCCCEEECCHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
GVDITKLGDTQLIKQADVVMLMLLLGEEFDEETKRINYEYYEKRTMHKSSLGPSMYAIMG
CCCEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
LKVGDHKNAYQSFIRSANVDLVDNQGNTKEGLHAASAGGTWQVAVFGFGGMEIDKEGALN
EEECCCHHHHHHHHHCCCCEEEECCCCCCCCCEECCCCCEEEEEEEECCCEEECCCCCCC
INSWLPEKWDKLSYKVFWKGNLIEVIVTKEDVSVKKLEGKGNIKVKVKGKEITLQ
CHHCCCCCCCCCCEEEEEECCEEEEEEECCCCCEEEECCCCCEEEEEECCEEEEC
>Mature Secondary Structure
MVKHMFLEDVNNLISDDKWLIFQNEYNTEVNPRYETLFTLTNGYMGVRGTFEEGSEGERS
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCC
GNFIAGIFGKSDAQVREIVNAQNWLRIKLYVEGEELSLDKCQLIEFKRILDMKKGILFRS
CCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
MLIKDSKDRITRIEGYRFISRSDLHRSAIKLFVTPVNYSGVVGIESIIDGTVLNSADSPK
HHHCCCHHHHHHCCCEEEECHHHHHHEEEEEEEEECCCCCCEEHHHHHCCCCCCCCCCCH
HRVKHLKVADNSSLNKSGVYLETATIDDDIRIATGSAVRLYHYEDKEKNNIAKFKRFLPL
HHHHEEEECCCCCCCCCCEEEEEEECCCCEEEECCCEEEEEEECCCCCCCHHHHHHHCCC
GEMSIEYFEFDGTENKTVVIDKFIITYTSRDVKKGLLKSTVEKELFAFAGEGIDKELQRH
CCCEEEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEVYEELWSVADINIEGDEEADKALRFNIFHLMSSVNENDPMVSIAAKALHGEGYKGHVF
HHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCEEEEEE
WDTEIFMLPFFIYVHPKAAKTLLMYRYNMLDAARKNAALNGYKGAQYPWESADTGEEETP
ECCHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
KWGFDYMGNPVRIWTGDLEHHITADIAFAVWEYFRATEDIEFMLNYGAEIIFETARFWVS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHH
RCEYVKELDRYEINNVIGPDEFHEHVDNNAYTNYLAKWNIKKGLELINMLKEKYPEHYHA
HHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHEEECCHHHHHHHHHHHHHHCCHHHHH
ISNKICLTNEEMEKWKEVEEKIYIPYDKDKKLIEQFEGYFDKKDYVIDKFDENNMPIWPE
HCCCEEECCHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
GVDITKLGDTQLIKQADVVMLMLLLGEEFDEETKRINYEYYEKRTMHKSSLGPSMYAIMG
CCCEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
LKVGDHKNAYQSFIRSANVDLVDNQGNTKEGLHAASAGGTWQVAVFGFGGMEIDKEGALN
EEECCCHHHHHHHHHCCCCEEEECCCCCCCCCEECCCCCEEEEEEEECCCEEECCCCCCC
INSWLPEKWDKLSYKVFWKGNLIEVIVTKEDVSVKKLEGKGNIKVKVKGKEITLQ
CHHCCCCCCCCCCEEEEEECCEEEEEEECCCCCEEEECCCCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA