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The map label for this gene is tilS [H]

Identifier: 162448219

GI number: 162448219

Start: 1445402

End: 1446622

Strand: Direct

Name: tilS [H]

Synonym: ACL_1384

Alternate gene names: 162448219

Gene position: 1445402-1446622 (Clockwise)

Preceding gene: 162448214

Following gene: 162448220

Centisome position: 96.55

GC content: 29.89

Gene sequence:

>1221_bases
ATGGCTTTAAAACTGAAACTTGATTCAACAAAATTATATGTTCTTGCAGTATCTGGAGGAGTTGACTCCATGGTATTGTT
TGATTTATTTGTTAAACATAATCTAAATTTTGTTGTTGTGCATTTTAATCACCAAAAGCGCGCTGAATCTATTTTAGATC
ACGAACTGGTTGAATCCATGTGTACACTTCATCATATTCCTTACCATTATATCAAATTAAAAATTAAAAATGGTAATTTT
CAGGAAGTGTCACGAAATGCTAGATATAAAAATCTAGAGTTTATTGCAGAACAATATCATACACCATATATCGTTACAGC
ACATCATTTAGATGATTTAGCTGAAACTATTATGATGAAGCTTATTAGAGGTTCTAATCTCTTAGGTTATGCTGCGATGC
AAGAAGTAACCCATATCGATAAATTCATCTATTTAAAACCTTTATTGAACTATAGTAAAGAAGAAATTAGTCTTTATCAA
CAAACACATCAAATTAAATTCTATGAAGATCATACAAACTTAGAAGATACTTACTCAAGAAATCGAGTAAGGCATCATCT
CATCCCTTTATTAAAGGAAGAAAACGATGTTTTAACACATTTAAAAAACTTTTCTAAACAAAGTTATTTAGCAGCATCCT
ACATTAGGAAGGAGTCCAATAACTTCTTAAGTAAGGATTTATCTTTTCATTTATCGAGTTTTGATGCACTACATGATGCT
GTTAAAATGGACGTTATTTCCTTAATATTTGAGTCTTTAAAAATCGATAAAACATTTAAGAAAATTGAAACTATTTTAGC
ACAATTAAAGTCAATAAAGCCTAATATTAATATCACGCTTAATAAGCACTATCATATGATAAAAGCCTATGATTTTGTCT
ACATAGAGCATATTGATAAAAATAAGACTCGAAATGATGAGAATTGTCTCATGATTTCCCACAATAAAGTAGACCTCCCG
AAGATTTGGGTAGAATTATGTTATAATAAACTGGATTTTCCTATTCAATTAAGGAAGCGTAAACCGGGGGATCGTTTATC
CTTTACCTACGGTAGTAAGAAATTAAAGGACTATTTAATTGATAAGAAGATTGCAATTAGTGATAGAAATAATCTAAATG
TCGTTGTGGATAGTTCCGGTACCATTTTATGGATACCTGGACTTTATTTAAATGAGACCCTAGGGTCAGAACATAAAATT
TACTTATCTATAAAGGAGTAA

Upstream 100 bases:

>100_bases
CTTTTCTTCTGGCTGAGCCAGAACCTAATATTAACATAATAATCTCTCCCTATACATGATATAATATTTAGTATATAAAT
TTGAAAAATGTGGTGAAAAA

Downstream 100 bases:

>100_bases
CTAAATATGCATCAAGATATTCTAGAAGTACTTGTAAGTGAATCACAGATTAAAGAGATTACAGAAAAGTTAGGCAAGCA
ACTCACTCATGATTATGCTG

Product: PP-loop superfamily ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MALKLKLDSTKLYVLAVSGGVDSMVLFDLFVKHNLNFVVVHFNHQKRAESILDHELVESMCTLHHIPYHYIKLKIKNGNF
QEVSRNARYKNLEFIAEQYHTPYIVTAHHLDDLAETIMMKLIRGSNLLGYAAMQEVTHIDKFIYLKPLLNYSKEEISLYQ
QTHQIKFYEDHTNLEDTYSRNRVRHHLIPLLKEENDVLTHLKNFSKQSYLAASYIRKESNNFLSKDLSFHLSSFDALHDA
VKMDVISLIFESLKIDKTFKKIETILAQLKSIKPNINITLNKHYHMIKAYDFVYIEHIDKNKTRNDENCLMISHNKVDLP
KIWVELCYNKLDFPIQLRKRKPGDRLSFTYGSKKLKDYLIDKKIAISDRNNLNVVVDSSGTILWIPGLYLNETLGSEHKI
YLSIKE

Sequences:

>Translated_406_residues
MALKLKLDSTKLYVLAVSGGVDSMVLFDLFVKHNLNFVVVHFNHQKRAESILDHELVESMCTLHHIPYHYIKLKIKNGNF
QEVSRNARYKNLEFIAEQYHTPYIVTAHHLDDLAETIMMKLIRGSNLLGYAAMQEVTHIDKFIYLKPLLNYSKEEISLYQ
QTHQIKFYEDHTNLEDTYSRNRVRHHLIPLLKEENDVLTHLKNFSKQSYLAASYIRKESNNFLSKDLSFHLSSFDALHDA
VKMDVISLIFESLKIDKTFKKIETILAQLKSIKPNINITLNKHYHMIKAYDFVYIEHIDKNKTRNDENCLMISHNKVDLP
KIWVELCYNKLDFPIQLRKRKPGDRLSFTYGSKKLKDYLIDKKIAISDRNNLNVVVDSSGTILWIPGLYLNETLGSEHKI
YLSIKE
>Mature_405_residues
ALKLKLDSTKLYVLAVSGGVDSMVLFDLFVKHNLNFVVVHFNHQKRAESILDHELVESMCTLHHIPYHYIKLKIKNGNFQ
EVSRNARYKNLEFIAEQYHTPYIVTAHHLDDLAETIMMKLIRGSNLLGYAAMQEVTHIDKFIYLKPLLNYSKEEISLYQQ
THQIKFYEDHTNLEDTYSRNRVRHHLIPLLKEENDVLTHLKNFSKQSYLAASYIRKESNNFLSKDLSFHLSSFDALHDAV
KMDVISLIFESLKIDKTFKKIETILAQLKSIKPNINITLNKHYHMIKAYDFVYIEHIDKNKTRNDENCLMISHNKVDLPK
IWVELCYNKLDFPIQLRKRKPGDRLSFTYGSKKLKDYLIDKKIAISDRNNLNVVVDSSGTILWIPGLYLNETLGSEHKIY
LSIKE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=202, Percent_Identity=28.7128712871287, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47602; Mature: 47471

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKLKLDSTKLYVLAVSGGVDSMVLFDLFVKHNLNFVVVHFNHQKRAESILDHELVESM
CEEEEEECCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
CTLHHIPYHYIKLKIKNGNFQEVSRNARYKNLEFIAEQYHTPYIVTAHHLDDLAETIMMK
HHHHCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
LIRGSNLLGYAAMQEVTHIDKFIYLKPLLNYSKEEISLYQQTHQIKFYEDHTNLEDTYSR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCHHHHHH
NRVRHHLIPLLKEENDVLTHLKNFSKQSYLAASYIRKESNNFLSKDLSFHLSSFDALHDA
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VKMDVISLIFESLKIDKTFKKIETILAQLKSIKPNINITLNKHYHMIKAYDFVYIEHIDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEEEEEECCC
NKTRNDENCLMISHNKVDLPKIWVELCYNKLDFPIQLRKRKPGDRLSFTYGSKKLKDYLI
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCHHHHHHHC
DKKIAISDRNNLNVVVDSSGTILWIPGLYLNETLGSEHKIYLSIKE
CCEEEEECCCCEEEEEECCCCEEEECCCEEECCCCCCEEEEEEEEC
>Mature Secondary Structure 
ALKLKLDSTKLYVLAVSGGVDSMVLFDLFVKHNLNFVVVHFNHQKRAESILDHELVESM
EEEEEECCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
CTLHHIPYHYIKLKIKNGNFQEVSRNARYKNLEFIAEQYHTPYIVTAHHLDDLAETIMMK
HHHHCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
LIRGSNLLGYAAMQEVTHIDKFIYLKPLLNYSKEEISLYQQTHQIKFYEDHTNLEDTYSR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCHHHHHH
NRVRHHLIPLLKEENDVLTHLKNFSKQSYLAASYIRKESNNFLSKDLSFHLSSFDALHDA
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VKMDVISLIFESLKIDKTFKKIETILAQLKSIKPNINITLNKHYHMIKAYDFVYIEHIDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEEEEEECCC
NKTRNDENCLMISHNKVDLPKIWVELCYNKLDFPIQLRKRKPGDRLSFTYGSKKLKDYLI
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCHHHHHHHC
DKKIAISDRNNLNVVVDSSGTILWIPGLYLNETLGSEHKIYLSIKE
CCEEEEECCCCEEEEEECCCCEEEECCCEEECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA