Click here to switch to the map view.

The map label for this gene is hpt [H]

Identifier: 162448220

GI number: 162448220

Start: 1446629

End: 1447168

Strand: Direct

Name: hpt [H]

Synonym: ACL_1385

Alternate gene names: 162448220

Gene position: 1446629-1447168 (Clockwise)

Preceding gene: 162448219

Following gene: 162448221

Centisome position: 96.64

GC content: 32.22

Gene sequence:

>540_bases
ATGCATCAAGATATTCTAGAAGTACTTGTAAGTGAATCACAGATTAAAGAGATTACAGAAAAGTTAGGCAAGCAACTCAC
TCATGATTATGCTGGTAAGAACGCATTATTTGTAGGACTATTAAATGGTTGTGTACCATTTATGTCTGAACTTGTCTTAA
AAATTGATACGCATATTGAAATTCAGTTCATGGCAGTATCTAGTTATCACGGTGGTATTAAGTCTTCTGGAGATGTTAAA
ATCAAATATGATTTAAACAGTCCAGTTCAAGATAGAGATGTTGTCATCGTAGAAGATATTGTTGATACCGGTGCAACTTT
AAAAACAGTTACTGAATTATTAAAATACCGTGGTGCAAAATCAGTAAAGGTTGTTACTTTATTAGATAAACCTTCTGGTA
GAAAAGTTGATTTTATACCAGATTATATTGGAGTTACTATCCCTAAGAAGTTTGTTGTGGGATATGGACTAGACTACAAT
GAATATTATAGAAACTTACCATACATCGGGGTATTAAAACCCGAAGTATATACAAAATAG

Upstream 100 bases:

>100_bases
GGATAGTTCCGGTACCATTTTATGGATACCTGGACTTTATTTAAATGAGACCCTAGGGTCAGAACATAAAATTTACTTAT
CTATAAAGGAGTAACTAAAT

Downstream 100 bases:

>100_bases
GAAGGAAACAATATGAATAAACAACAAAAACCTAAAAGAAGCCCGCTTCGTCCGGACTATCTTGTAATTGTAATTATTAT
ACTTTTAGCAATTGGTATGT

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MHQDILEVLVSESQIKEITEKLGKQLTHDYAGKNALFVGLLNGCVPFMSELVLKIDTHIEIQFMAVSSYHGGIKSSGDVK
IKYDLNSPVQDRDVVIVEDIVDTGATLKTVTELLKYRGAKSVKVVTLLDKPSGRKVDFIPDYIGVTIPKKFVVGYGLDYN
EYYRNLPYIGVLKPEVYTK

Sequences:

>Translated_179_residues
MHQDILEVLVSESQIKEITEKLGKQLTHDYAGKNALFVGLLNGCVPFMSELVLKIDTHIEIQFMAVSSYHGGIKSSGDVK
IKYDLNSPVQDRDVVIVEDIVDTGATLKTVTELLKYRGAKSVKVVTLLDKPSGRKVDFIPDYIGVTIPKKFVVGYGLDYN
EYYRNLPYIGVLKPEVYTK
>Mature_179_residues
MHQDILEVLVSESQIKEITEKLGKQLTHDYAGKNALFVGLLNGCVPFMSELVLKIDTHIEIQFMAVSSYHGGIKSSGDVK
IKYDLNSPVQDRDVVIVEDIVDTGATLKTVTELLKYRGAKSVKVVTLLDKPSGRKVDFIPDYIGVTIPKKFVVGYGLDYN
EYYRNLPYIGVLKPEVYTK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:
GO:0004422: Hypoxanthine-guanine phosphoribosyltransferase
GO:0005737: Hypoxanthine-guanine phosphoribosyltransferase
GO:0006166: Hypoxanthine-guanine phosphoribosyltransferase
GO:0009116: Hypoxanthine-guanine phosphoribosyltransferase
GO:0016740: Hypoxanthine-guanine phosphoribosyltransferase
GO:0016757: Hypoxanthine-guanine phosphoribosyltransferase

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=180, Percent_Identity=35, Blast_Score=104, Evalue=4e-23,
Organism=Homo sapiens, GI9910262, Length=182, Percent_Identity=32.4175824175824, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI87081689, Length=167, Percent_Identity=46.1077844311377, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17510817, Length=179, Percent_Identity=30.1675977653631, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20043; Mature: 20043

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQDILEVLVSESQIKEITEKLGKQLTHDYAGKNALFVGLLNGCVPFMSELVLKIDTHIE
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCEE
IQFMAVSSYHGGIKSSGDVKIKYDLNSPVQDRDVVIVEDIVDTGATLKTVTELLKYRGAK
EEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCC
SVKVVTLLDKPSGRKVDFIPDYIGVTIPKKFVVGYGLDYNEYYRNLPYIGVLKPEVYTK
CEEEEEEEECCCCCEEECCCCHHCCCCCCCEEEECCCCHHHHHHCCCEEEEECCHHCCC
>Mature Secondary Structure
MHQDILEVLVSESQIKEITEKLGKQLTHDYAGKNALFVGLLNGCVPFMSELVLKIDTHIE
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCEE
IQFMAVSSYHGGIKSSGDVKIKYDLNSPVQDRDVVIVEDIVDTGATLKTVTELLKYRGAK
EEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCC
SVKVVTLLDKPSGRKVDFIPDYIGVTIPKKFVVGYGLDYNEYYRNLPYIGVLKPEVYTK
CEEEEEEEECCCCCEEECCCCHHCCCCCCCEEEECCCCHHHHHHCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]