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The map label for this gene is pfk2 [H]

Identifier: 162447946

GI number: 162447946

Start: 1128970

End: 1130214

Strand: Direct

Name: pfk2 [H]

Synonym: ACL_1092

Alternate gene names: 162447946

Gene position: 1128970-1130214 (Clockwise)

Preceding gene: 162447939

Following gene: 162447947

Centisome position: 75.42

GC content: 34.54

Gene sequence:

>1245_bases
ATGAAAAAATTAGTTGGTGCTGCACTCTTAGGACAATCTGGAGGTCCAACCTCAGTGATTAACGCCTCAGCAGCAGGTGT
ATTTTTAGAGAGTTTAAAGCATGATGAGATTACAGAAATCTATGGTGCTCAACACGGTATCAAAGGTATCTTAGATGAAA
ACTTCTATGATATTAGAAAAGAAGATGTTGAAGAATTAGAAAGATTAAAGTATACTCCATCATCTGCAATTGGTTCAGTA
AGATACAAATTAAAAGATTACAAAAAAGATCCAACAGACTATAATAAATTATTAGAAATCTTTAAAAAATATAACATTCG
TTATTTCTTCTACAATGGTGGAAATGACTCAATGGATACATGTTTAAAAATTTCTAAATTTATGAAAACAGCTGGTTGGG
AAATGCGCGTTGTTGGGGTACCTAAAACAATCGATAACGACTTAGCAGGTATCGACCATTCACCTGGATATGGTTCAGCA
GCTAAATACGTTGCAACTTCTATCATGGAATTATACTTAGATGCTACAGTTTATAACACACAACAATTCATTATTGTTGA
AGTAATGGGTAGAAATGCTGGTTGGTTAACTGCTGCTGCTGCACTTGCTTCAGTTGGTGGTGTTAAACCAGACTTAATGT
ATTTACCAGAAACTCCATTTGATATGGACAAATTCTACAACCAAGTAGGTGAATTACTAAAAACTGGTAAATCAGTATTT
GTAGTTGTATCAGAAGGTATTAAAACTGCTGAAGGTAAATACATCCCTGAATATGAAACAGAAATCGCTCGTGATGCATT
CGGACATGCGAACTTAGGTGGTACTGCAAGTGTACTTGCAAACCACGTAGGTGAAAAATTCGGTGTTAAGACTCGTGCTA
TCGAATTCTCATTATTACAAAGATCAGCTGCTCACATTGCATCTCAAACAGATATCAATGAAGCATTCAAAGTTGGACAA
ATTGCTGTTAAAAAAGCAATCAATGGTACAACAAACAAATTTGTAGGTATCCAAAGAGTTCCTGGGGATAAATACAAAGT
GAAGTATCCTCTACTTCCATTAAAGATTGTTGCTAACGCAGAACGTAAAGTACCATTAGAGTGGATCTTACCTGATGGAC
AAGGTTTAACTCAAGACTTTATTGATTATGCATTACCATTAATCCAAGGTGAAACTAAATTAGAAAAAGTAAACGGGTTA
CCTCGTTTTGCTAAATTAAATAAAGTTTTAGCACCTAAAAAATAA

Upstream 100 bases:

>100_bases
TAATTGAACATTTAACGCTTAAGTGTTTGCAATAAAGGGTATTCTTTGATAAACTTATTAGTGGAAATTTACTAATAAAT
AATAAAAAGGACGGTATACT

Downstream 100 bases:

>100_bases
GTTCTAAATTGTAGAAGCCTAACCAATTTCGGTTAGGCTTCTTTCTTTTTAAACGCTTCTAAAGTCTTTTCATCTTATAT
TTGATACAATATATATAACA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKKLVGAALLGQSGGPTSVINASAAGVFLESLKHDEITEIYGAQHGIKGILDENFYDIRKEDVEELERLKYTPSSAIGSV
RYKLKDYKKDPTDYNKLLEIFKKYNIRYFFYNGGNDSMDTCLKISKFMKTAGWEMRVVGVPKTIDNDLAGIDHSPGYGSA
AKYVATSIMELYLDATVYNTQQFIIVEVMGRNAGWLTAAAALASVGGVKPDLMYLPETPFDMDKFYNQVGELLKTGKSVF
VVVSEGIKTAEGKYIPEYETEIARDAFGHANLGGTASVLANHVGEKFGVKTRAIEFSLLQRSAAHIASQTDINEAFKVGQ
IAVKKAINGTTNKFVGIQRVPGDKYKVKYPLLPLKIVANAERKVPLEWILPDGQGLTQDFIDYALPLIQGETKLEKVNGL
PRFAKLNKVLAPKK

Sequences:

>Translated_414_residues
MKKLVGAALLGQSGGPTSVINASAAGVFLESLKHDEITEIYGAQHGIKGILDENFYDIRKEDVEELERLKYTPSSAIGSV
RYKLKDYKKDPTDYNKLLEIFKKYNIRYFFYNGGNDSMDTCLKISKFMKTAGWEMRVVGVPKTIDNDLAGIDHSPGYGSA
AKYVATSIMELYLDATVYNTQQFIIVEVMGRNAGWLTAAAALASVGGVKPDLMYLPETPFDMDKFYNQVGELLKTGKSVF
VVVSEGIKTAEGKYIPEYETEIARDAFGHANLGGTASVLANHVGEKFGVKTRAIEFSLLQRSAAHIASQTDINEAFKVGQ
IAVKKAINGTTNKFVGIQRVPGDKYKVKYPLLPLKIVANAERKVPLEWILPDGQGLTQDFIDYALPLIQGETKLEKVNGL
PRFAKLNKVLAPKK
>Mature_414_residues
MKKLVGAALLGQSGGPTSVINASAAGVFLESLKHDEITEIYGAQHGIKGILDENFYDIRKEDVEELERLKYTPSSAIGSV
RYKLKDYKKDPTDYNKLLEIFKKYNIRYFFYNGGNDSMDTCLKISKFMKTAGWEMRVVGVPKTIDNDLAGIDHSPGYGSA
AKYVATSIMELYLDATVYNTQQFIIVEVMGRNAGWLTAAAALASVGGVKPDLMYLPETPFDMDKFYNQVGELLKTGKSVF
VVVSEGIKTAEGKYIPEYETEIARDAFGHANLGGTASVLANHVGEKFGVKTRAIEFSLLQRSAAHIASQTDINEAFKVGQ
IAVKKAINGTTNKFVGIQRVPGDKYKVKYPLLPLKIVANAERKVPLEWILPDGQGLTQDFIDYALPLIQGETKLEKVNGL
PRFAKLNKVLAPKK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:
GO:0003872: 6-phosphofructokinase
GO:0005945: 6-phosphofructokinase
GO:0006096: 6-phosphofructokinase
GO:0016301: 6-phosphofructokinase
GO:0016310: 6-phosphofructokinase
GO:0016740: 6-phosphofructokinase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=326, Percent_Identity=25.1533742331288, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=26.1290322580645, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25147584, Length=172, Percent_Identity=28.4883720930233, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 45627; Mature: 45627

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVGAALLGQSGGPTSVINASAAGVFLESLKHDEITEIYGAQHGIKGILDENFYDIRK
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHCCCHHHHHHCCHHCHHHHHCCCHHHHHH
EDVEELERLKYTPSSAIGSVRYKLKDYKKDPTDYNKLLEIFKKYNIRYFFYNGGNDSMDT
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHH
CLKISKFMKTAGWEMRVVGVPKTIDNDLAGIDHSPGYGSAAKYVATSIMELYLDATVYNT
HHHHHHHHHHCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECC
QQFIIVEVMGRNAGWLTAAAALASVGGVKPDLMYLPETPFDMDKFYNQVGELLKTGKSVF
CEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEE
VVVSEGIKTAEGKYIPEYETEIARDAFGHANLGGTASVLANHVGEKFGVKTRAIEFSLLQ
EEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
RSAAHIASQTDINEAFKVGQIAVKKAINGTTNKFVGIQRVPGDKYKVKYPLLPLKIVANA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEEECCCEEEEEEECC
ERKVPLEWILPDGQGLTQDFIDYALPLIQGETKLEKVNGLPRFAKLNKVLAPKK
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKKLVGAALLGQSGGPTSVINASAAGVFLESLKHDEITEIYGAQHGIKGILDENFYDIRK
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHCCCHHHHHHCCHHCHHHHHCCCHHHHHH
EDVEELERLKYTPSSAIGSVRYKLKDYKKDPTDYNKLLEIFKKYNIRYFFYNGGNDSMDT
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHH
CLKISKFMKTAGWEMRVVGVPKTIDNDLAGIDHSPGYGSAAKYVATSIMELYLDATVYNT
HHHHHHHHHHCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECC
QQFIIVEVMGRNAGWLTAAAALASVGGVKPDLMYLPETPFDMDKFYNQVGELLKTGKSVF
CEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEE
VVVSEGIKTAEGKYIPEYETEIARDAFGHANLGGTASVLANHVGEKFGVKTRAIEFSLLQ
EEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
RSAAHIASQTDINEAFKVGQIAVKKAINGTTNKFVGIQRVPGDKYKVKYPLLPLKIVANA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEEECCCEEEEEEECC
ERKVPLEWILPDGQGLTQDFIDYALPLIQGETKLEKVNGLPRFAKLNKVLAPKK
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]