Click here to switch to the map view.

The map label for this gene is gdh [H]

Identifier: 162447947

GI number: 162447947

Start: 1130335

End: 1131672

Strand: Direct

Name: gdh [H]

Synonym: ACL_1093

Alternate gene names: 162447947

Gene position: 1130335-1131672 (Clockwise)

Preceding gene: 162447946

Following gene: 162447948

Centisome position: 75.51

GC content: 33.71

Gene sequence:

>1338_bases
ATGATGCATGAACACTTAACATCTATTTATAATGAATTACAGACTAAATATAGTTATCAAAAAGAGTATCTTCAATCTGT
ATATGAATTCTTTGAATCTATTTCAGAGGTTATTGAAGAAGATCCTAGAATCATTGAAACAAATATTATACGTAACATTG
TAGAACCAGATCGTATTATCACCTTTAAAGTTCCTTGGGAAGATGATAAAGGTGCTAGACATGTAAATACAGGTTACCGA
GTACAGTTTAACCAAGCACTAGGTCCATATAAAGGTGGTATTAGATTTACACCTAAAGTCAATGAATCCATACTAAAATT
TTTAGGATTTGAACAAACCTTTAAAAACAGTTTAACAGGTATACCTTTAGGCGGGGCTAAAGGTGGCGCTGATTTTGATC
CAAAACATAAAAGTGACCAAGAGATCCTAAGATTTTGTAGATCTTATATGATGGAACTACATAAGTTTTTAGGTCCCAAC
ATGGATATTCCAGCAGGAGACCTAGGGGTTTCACAAAAAACAGTCGGTTATATGTTTGGGATGTATAAACGCTTAACTCA
AAGACATGAAGGCGTATTTACTGGTAAGGGCACTAAATATGGTGGTTCCTTAGCTAGAACTGAAGCTACAGGTTACGGTA
TTATTTATATGGTACTTGCTGCTTTAAAAACTTACTTTCAAACAGATTTAAAAGACAAAATAGTTATTATATCCGGTAGT
GGTAATGTTGCCATACATACAGGGTATAAGGCTCAAGCAATGGGTGCTAAAGTGGTTGCTATGTCTTCTATTGATGGTGT
GATACACCATGAAACTGGTATTGACGTGTCTTACTTAGAATATATTAAAGATAATAATTTATCAATTAAAGATTATACTT
TACGTTATCCTTCGGCAATTTTCGCAGAAAATCCTAAACGCATTTGGGAAATTAACTGTGATATTGCACTACCTTGTGCA
ACCGAAAATGAATTAGATTTAGAGGAAGCTAAAAAGTTAGTAGATAATGGCATTATGTTACTTGCAGAGGGTGCGAATAT
GCCAACTACTTTAGATGCTACACACTATTTTCTTGATAACAACATTCTATTCATTCCAGGTAAGGCATGTAATGCAGGTG
GTGTATCGGTCAGTGGACTAGAGATGCAACAAAATGCATTACATACTTATTGGGATTTTGATACAGTTGATCAAAAACTA
CAAACTATCATGAATCGTATTTTCGAAGATATGTATGAAACTGCTAGAAGTTATGGCGATGATAAAAATTTAGTTAAGGG
TGCAAATATTTTATCCTTTAAACGAGTTTATCAAGCTATGCTTGAACAAGGTCTATAA

Upstream 100 bases:

>100_bases
AACCAATTTCGGTTAGGCTTCTTTCTTTTTAAACGCTTCTAAAGTCTTTTCATCTTATATTTGATACAATATATATAACA
ATAAAGGAGCCAACTTACTT

Downstream 100 bases:

>100_bases
AAAGAAGCATAATCTTATGATACTCGTAACTTAAGATTATGCTTTTTATGTTATAATTTTTATTAACAAATGAAAATGAA
TGAGGCTTAACTTTGAAAGA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH; Surface-associated protein PGAG1 [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MMHEHLTSIYNELQTKYSYQKEYLQSVYEFFESISEVIEEDPRIIETNIIRNIVEPDRIITFKVPWEDDKGARHVNTGYR
VQFNQALGPYKGGIRFTPKVNESILKFLGFEQTFKNSLTGIPLGGAKGGADFDPKHKSDQEILRFCRSYMMELHKFLGPN
MDIPAGDLGVSQKTVGYMFGMYKRLTQRHEGVFTGKGTKYGGSLARTEATGYGIIYMVLAALKTYFQTDLKDKIVIISGS
GNVAIHTGYKAQAMGAKVVAMSSIDGVIHHETGIDVSYLEYIKDNNLSIKDYTLRYPSAIFAENPKRIWEINCDIALPCA
TENELDLEEAKKLVDNGIMLLAEGANMPTTLDATHYFLDNNILFIPGKACNAGGVSVSGLEMQQNALHTYWDFDTVDQKL
QTIMNRIFEDMYETARSYGDDKNLVKGANILSFKRVYQAMLEQGL

Sequences:

>Translated_445_residues
MMHEHLTSIYNELQTKYSYQKEYLQSVYEFFESISEVIEEDPRIIETNIIRNIVEPDRIITFKVPWEDDKGARHVNTGYR
VQFNQALGPYKGGIRFTPKVNESILKFLGFEQTFKNSLTGIPLGGAKGGADFDPKHKSDQEILRFCRSYMMELHKFLGPN
MDIPAGDLGVSQKTVGYMFGMYKRLTQRHEGVFTGKGTKYGGSLARTEATGYGIIYMVLAALKTYFQTDLKDKIVIISGS
GNVAIHTGYKAQAMGAKVVAMSSIDGVIHHETGIDVSYLEYIKDNNLSIKDYTLRYPSAIFAENPKRIWEINCDIALPCA
TENELDLEEAKKLVDNGIMLLAEGANMPTTLDATHYFLDNNILFIPGKACNAGGVSVSGLEMQQNALHTYWDFDTVDQKL
QTIMNRIFEDMYETARSYGDDKNLVKGANILSFKRVYQAMLEQGL
>Mature_445_residues
MMHEHLTSIYNELQTKYSYQKEYLQSVYEFFESISEVIEEDPRIIETNIIRNIVEPDRIITFKVPWEDDKGARHVNTGYR
VQFNQALGPYKGGIRFTPKVNESILKFLGFEQTFKNSLTGIPLGGAKGGADFDPKHKSDQEILRFCRSYMMELHKFLGPN
MDIPAGDLGVSQKTVGYMFGMYKRLTQRHEGVFTGKGTKYGGSLARTEATGYGIIYMVLAALKTYFQTDLKDKIVIISGS
GNVAIHTGYKAQAMGAKVVAMSSIDGVIHHETGIDVSYLEYIKDNNLSIKDYTLRYPSAIFAENPKRIWEINCDIALPCA
TENELDLEEAKKLVDNGIMLLAEGANMPTTLDATHYFLDNNILFIPGKACNAGGVSVSGLEMQQNALHTYWDFDTVDQKL
QTIMNRIFEDMYETARSYGDDKNLVKGANILSFKRVYQAMLEQGL

Specific function: Probably involved in degradation rather than biosynthesis of glutamate [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cell surface [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=412, Percent_Identity=31.7961165048544, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI4885281, Length=412, Percent_Identity=31.5533980582524, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=45.7207207207207, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17544676, Length=371, Percent_Identity=31.5363881401617, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=44.273127753304, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6324951, Length=449, Percent_Identity=44.3207126948775, Blast_Score=329, Evalue=4e-91,
Organism=Drosophila melanogaster, GI45549226, Length=355, Percent_Identity=32.3943661971831, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI45553475, Length=355, Percent_Identity=32.3943661971831, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24649283, Length=363, Percent_Identity=32.2314049586777, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=31.6326530612245, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 50189; Mature: 50189

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHEHLTSIYNELQTKYSYQKEYLQSVYEFFESISEVIEEDPRIIETNIIRNIVEPDRII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCEEE
TFKVPWEDDKGARHVNTGYRVQFNQALGPYKGGIRFTPKVNESILKFLGFEQTFKNSLTG
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHCCCC
IPLGGAKGGADFDPKHKSDQEILRFCRSYMMELHKFLGPNMDIPAGDLGVSQKTVGYMFG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
MYKRLTQRHEGVFTGKGTKYGGSLARTEATGYGIIYMVLAALKTYFQTDLKDKIVIISGS
HHHHHHHHHCCEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
GNVAIHTGYKAQAMGAKVVAMSSIDGVIHHETGIDVSYLEYIKDNNLSIKDYTLRYPSAI
CCEEEEECCCCHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCHH
FAENPKRIWEINCDIALPCATENELDLEEAKKLVDNGIMLLAEGANMPTTLDATHYFLDN
HCCCCCEEEEEECCEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCEECCEEEEECC
NILFIPGKACNAGGVSVSGLEMQQNALHTYWDFDTVDQKLQTIMNRIFEDMYETARSYGD
CEEEECCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKNLVKGANILSFKRVYQAMLEQGL
CCHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMHEHLTSIYNELQTKYSYQKEYLQSVYEFFESISEVIEEDPRIIETNIIRNIVEPDRII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCEEE
TFKVPWEDDKGARHVNTGYRVQFNQALGPYKGGIRFTPKVNESILKFLGFEQTFKNSLTG
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHCCCC
IPLGGAKGGADFDPKHKSDQEILRFCRSYMMELHKFLGPNMDIPAGDLGVSQKTVGYMFG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
MYKRLTQRHEGVFTGKGTKYGGSLARTEATGYGIIYMVLAALKTYFQTDLKDKIVIISGS
HHHHHHHHHCCEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
GNVAIHTGYKAQAMGAKVVAMSSIDGVIHHETGIDVSYLEYIKDNNLSIKDYTLRYPSAI
CCEEEEECCCCHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCHH
FAENPKRIWEINCDIALPCATENELDLEEAKKLVDNGIMLLAEGANMPTTLDATHYFLDN
HCCCCCEEEEEECCEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCEECCEEEEECC
NILFIPGKACNAGGVSVSGLEMQQNALHTYWDFDTVDQKLQTIMNRIFEDMYETARSYGD
CEEEECCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKNLVKGANILSFKRVYQAMLEQGL
CCHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8132343 [H]