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The map label for this gene is pflA [H]

Identifier: 162446916

GI number: 162446916

Start: 34621

End: 35382

Strand: Direct

Name: pflA [H]

Synonym: ACL_0029

Alternate gene names: 162446916

Gene position: 34621-35382 (Clockwise)

Preceding gene: 162446915

Following gene: 162446917

Centisome position: 2.31

GC content: 31.76

Gene sequence:

>762_bases
ATGAAGGATTCTAATCTACTAGAAGTAGTAGGTAATGTACACTCAATAGAAACATTTGGTGCCTTTGATGGACCAGGACT
TAGATATGTATTATTCTTACAAGGTTGTCCTTTAAGATGTAAATTCTGTCATAACAGAGACACTTGGGGCACAGAAGATA
ATAAGTTAATGACCGTAGAAGAGATATTAAACGACTATAATAAATATAGAGCATTCTATAAAAAAGGTGGTTTAACAGTA
TCAGGTGGCGAAGCTACCTTACAAATCGGGTTTTTAACTGCATTATTCAAAGAAGCTAAAAATAGAAATATACATACGTG
TTTAGATACATCAGCAGGTACATTTAGTGAAGCAAGATTACCTGAGTTTGAAGAACTTCTCAAATACACAGATCTTGTGT
TACTAGATATTAAACATATTGATGATGAACGTCACAAATGGTTAACCGGTGCAAGCAATAAAAACATATTAAAGTTTGCA
AGACTGTTAAGTGATAAAAAAATACCTACGATATTAAGACATATACTCTTACCACAAATTAACTCGCAAGATGTTTACTT
AGAAAGACTAAGAACATTCATTGACTCTTTAGATAACTTTATTGGTATTGACATTCTTCCTTATCATACTAAAGGTATTA
TGAAGTGGGATAACATGGGTATTGAATATGAACTTAAAGATACACCAGAACCTACTAAAGAAGAGGTTTTAAGAGCAGAG
CATATACTTAAAGACGACTATAAGTATATGAAACTAAATTAG

Upstream 100 bases:

>100_bases
GAATTATATCCAAACCTTACAATTAGAGTATCAGGTTATGCTGTAAACTTCTCAAATCTAACGAATGAACAAAAATTAGA
AGTTATGAAGCGTACATTCC

Downstream 100 bases:

>100_bases
TTCCTAATTAAGGGATAACATAGCATCTTAAACAGATGATATGTTATCTCTTTTACTTTATATGTTGTTTTCTAATATAA
CCTCTAAGGAAATTGAATGT

Product: pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKDSNLLEVVGNVHSIETFGAFDGPGLRYVLFLQGCPLRCKFCHNRDTWGTEDNKLMTVEEILNDYNKYRAFYKKGGLTV
SGGEATLQIGFLTALFKEAKNRNIHTCLDTSAGTFSEARLPEFEELLKYTDLVLLDIKHIDDERHKWLTGASNKNILKFA
RLLSDKKIPTILRHILLPQINSQDVYLERLRTFIDSLDNFIGIDILPYHTKGIMKWDNMGIEYELKDTPEPTKEEVLRAE
HILKDDYKYMKLN

Sequences:

>Translated_253_residues
MKDSNLLEVVGNVHSIETFGAFDGPGLRYVLFLQGCPLRCKFCHNRDTWGTEDNKLMTVEEILNDYNKYRAFYKKGGLTV
SGGEATLQIGFLTALFKEAKNRNIHTCLDTSAGTFSEARLPEFEELLKYTDLVLLDIKHIDDERHKWLTGASNKNILKFA
RLLSDKKIPTILRHILLPQINSQDVYLERLRTFIDSLDNFIGIDILPYHTKGIMKWDNMGIEYELKDTPEPTKEEVLRAE
HILKDDYKYMKLN
>Mature_253_residues
MKDSNLLEVVGNVHSIETFGAFDGPGLRYVLFLQGCPLRCKFCHNRDTWGTEDNKLMTVEEILNDYNKYRAFYKKGGLTV
SGGEATLQIGFLTALFKEAKNRNIHTCLDTSAGTFSEARLPEFEELLKYTDLVLLDIKHIDDERHKWLTGASNKNILKFA
RLLSDKKIPTILRHILLPQINSQDVYLERLRTFIDSLDNFIGIDILPYHTKGIMKWDNMGIEYELKDTPEPTKEEVLRAE
HILKDDYKYMKLN

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=42.5, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1790839, Length=250, Percent_Identity=32, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 29264; Mature: 29264

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSNLLEVVGNVHSIETFGAFDGPGLRYVLFLQGCPLRCKFCHNRDTWGTEDNKLMTVE
CCCCHHHHHHCCHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEEHH
EILNDYNKYRAFYKKGGLTVSGGEATLQIGFLTALFKEAKNRNIHTCLDTSAGTFSEARL
HHHHHHHHHHHHHHCCCEEEECCCEEEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
PEFEELLKYTDLVLLDIKHIDDERHKWLTGASNKNILKFARLLSDKKIPTILRHILLPQI
CHHHHHHHHHHEEEEEHHHCCCHHHHEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
NSQDVYLERLRTFIDSLDNFIGIDILPYHTKGIMKWDNMGIEYELKDTPEPTKEEVLRAE
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHH
HILKDDYKYMKLN
HHHHCCCEEEECC
>Mature Secondary Structure
MKDSNLLEVVGNVHSIETFGAFDGPGLRYVLFLQGCPLRCKFCHNRDTWGTEDNKLMTVE
CCCCHHHHHHCCHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEEHH
EILNDYNKYRAFYKKGGLTVSGGEATLQIGFLTALFKEAKNRNIHTCLDTSAGTFSEARL
HHHHHHHHHHHHHHCCCEEEECCCEEEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
PEFEELLKYTDLVLLDIKHIDDERHKWLTGASNKNILKFARLLSDKKIPTILRHILLPQI
CHHHHHHHHHHEEEEEHHHCCCHHHHEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
NSQDVYLERLRTFIDSLDNFIGIDILPYHTKGIMKWDNMGIEYELKDTPEPTKEEVLRAE
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHH
HILKDDYKYMKLN
HHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]