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The map label for this gene is pfl [H]

Identifier: 162446915

GI number: 162446915

Start: 32364

End: 34634

Strand: Direct

Name: pfl [H]

Synonym: ACL_0028

Alternate gene names: 162446915

Gene position: 32364-34634 (Clockwise)

Preceding gene: 162446913

Following gene: 162446916

Centisome position: 2.16

GC content: 33.6

Gene sequence:

>2271_bases
ATGGCTCAATCATGGAAAGGTTTTAAATCTGGTAACTGGGAAAAAGCAGTCGATGTTGCAGATTTCATAAAACAAAACTA
TTCAGAGTATACAGGAGACGGCACTTTTTTAGAAGGTGCAGTAGAAAGTTCTTTATCATTAAATGAATCTTTTAAAGATT
TATTGAAACTTGAAAAAGAAAAAGGTGGCGTAATTGATTTAGATACAAAGGTTGCATCTACAATCATTAGCCATGCGCCA
GGGTATATCGATAAAGATATCGAAAAAATTGTTGGTTTACAAACTGATGCACCATTAAAACGTGGATTCTTTCCTGAAGG
TGGAATTAACGTTGCTGTAAAATCCGTTGAAGCTTATGGTTATAAAGTAGAACAAGAAACAATTGATATTTATACAAACG
TTAGAAAAACACATAACCAAGGTGTATTTGATGCATATACTAAAGAAATTAGAAACGCTAGAAGTTCTCATATCATTACT
GGTTTACCAGACGGATATGGTAGAGGACGTATTATTGGAGATTATAGAAGAGTACCTCTTTATGGTGTAGACGCTTTAAT
CAAAGATAAATATGCACAACAAGAAGAATTAACATTCCCAATGTCTGAGCACAAAATCCGTTTAAGAGAAGAATTATCTG
ATCAAATTAAATCATTAAATGAATTAATTCAATTAGGAGATATGTACGGATTTGACTTAAGAAGACCTGCAAGTGACTCA
AAAGAAGCGATTCAATGGTTATACTTAGCTTATTTAGCAGCTGTTAAAGAACAAAACGGTGCGGCAATGAGTTTAGGTAG
AGTCGATGCATTCTTAGATATCTATATCGAAAGAGACTTAAAAGCTGCAAAATATAGCGAATTAGAAATTCAAGAATTTA
TTGACCATTTCGTCATGAAATTAAGAATGGTACGTTTTGCTAGAACTCCAGAATATAACCAATTATTTACAGGAGATCCA
ACATGGGTTACTGCTGTATTAGGTGGTATGTCAAATGATGGTAGATCACTTGTTACAAAAACAGCATTCAGATTATTACA
AACTTTATATAACCTAGGTACATCAGCAGAACCAAACCTGACTGTTTTATGGAGCAACAACCTTCCAGAAGGATTTAAAA
ACTTCACTGCGAAGGTAAGTATTGATACTTCAAGTATTCAATATGAAAATGATGATACAATTAGACCACTACATGGCGAT
GACTATGGTATCGCATGTTGCGTGTCACCAATGGCTATTGGTAAAGAAATGCAATTCTTTGGTGCACGTGCAAACATCGC
TAAAGCATTATTATATGCATTAAATGGTGGACGTGACGAAGTAAGTGGCAAACAAGTATTTGAAAAATCTAAGATGGAAG
TTTTAAAAACTGATGTATTAGATTATGATGTTGTGATGTCACGTTATGAAAAAACATTAGAAGAATTAGCTGAAGTTTAT
GTGAATGCTTTAAACATTATTCACTACATGCATGATAAATATGCATATGAAAAACTAGAAATGGCTTTACATGATCGTGA
TGTTAAGAGATTATTTGCAACTGGTATTGCAGGATTAAGCGTTGTTGCTGACTCATTATCAGCAATCAAATATGCAAAAG
TACACCCAATCTATGATGAAACATCAGGATTAATTACTGACTTTGAAACTGTTGGTGAATTCCCATGTTATGGTAATGAT
GATGATAGAGTTGATGATATTGCTGTAGATGTATTACGTAAATTCATGAGCTATATTAGAAAGAACCCAACATACAGAGA
ATCACATCCAACAATGAGTGTTTTAACGATTACATCTAACGTTGTATACGGTAAAAACACAGGTAATACACCAGATGGTC
GTCGTTATGGTGAACCATTTGCTCCAGGTGCTAACCCAATGCACGGTAGAGATAAATCAGGTGCATTAAAATCATTAATG
TCTGTTGCTAAATTACCATATGAAGATGCAGAAGACGGTATTTCAAATACATTTACAATTATTCCAAAAGCATTAGGTCA
TTCGATAAGTGCTGAAGGTTCTATTGTTCACCTTGTAGATGATGTACAAGTAAATAACCTTGTATCCTTATTAGATGGAT
ACTTCACATCTGGTGGATATCATTTAAACGTTAACGTGTTTAACAGAGAAACATTAAAAGCAGCTTATGACAACCCAGAA
TTATATCCAAACCTTACAATTAGAGTATCAGGTTATGCTGTAAACTTCTCAAATCTAACGAATGAACAAAAATTAGAAGT
TATGAAGCGTACATTCCATGAAGGATTCTAA

Upstream 100 bases:

>100_bases
TTTCATTTATAACATTCCAAGCAAAATGCTTTTGAAGGTTATAAAATGATAGTAAGAATACATGATTCATATGTAAACAT
TAATTAGGAGGAATGAAATC

Downstream 100 bases:

>100_bases
TCTACTAGAAGTAGTAGGTAATGTACACTCAATAGAAACATTTGGTGCCTTTGATGGACCAGGACTTAGATATGTATTAT
TCTTACAAGGTTGTCCTTTA

Product: pyruvate formate lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MAQSWKGFKSGNWEKAVDVADFIKQNYSEYTGDGTFLEGAVESSLSLNESFKDLLKLEKEKGGVIDLDTKVASTIISHAP
GYIDKDIEKIVGLQTDAPLKRGFFPEGGINVAVKSVEAYGYKVEQETIDIYTNVRKTHNQGVFDAYTKEIRNARSSHIIT
GLPDGYGRGRIIGDYRRVPLYGVDALIKDKYAQQEELTFPMSEHKIRLREELSDQIKSLNELIQLGDMYGFDLRRPASDS
KEAIQWLYLAYLAAVKEQNGAAMSLGRVDAFLDIYIERDLKAAKYSELEIQEFIDHFVMKLRMVRFARTPEYNQLFTGDP
TWVTAVLGGMSNDGRSLVTKTAFRLLQTLYNLGTSAEPNLTVLWSNNLPEGFKNFTAKVSIDTSSIQYENDDTIRPLHGD
DYGIACCVSPMAIGKEMQFFGARANIAKALLYALNGGRDEVSGKQVFEKSKMEVLKTDVLDYDVVMSRYEKTLEELAEVY
VNALNIIHYMHDKYAYEKLEMALHDRDVKRLFATGIAGLSVVADSLSAIKYAKVHPIYDETSGLITDFETVGEFPCYGND
DDRVDDIAVDVLRKFMSYIRKNPTYRESHPTMSVLTITSNVVYGKNTGNTPDGRRYGEPFAPGANPMHGRDKSGALKSLM
SVAKLPYEDAEDGISNTFTIIPKALGHSISAEGSIVHLVDDVQVNNLVSLLDGYFTSGGYHLNVNVFNRETLKAAYDNPE
LYPNLTIRVSGYAVNFSNLTNEQKLEVMKRTFHEGF

Sequences:

>Translated_756_residues
MAQSWKGFKSGNWEKAVDVADFIKQNYSEYTGDGTFLEGAVESSLSLNESFKDLLKLEKEKGGVIDLDTKVASTIISHAP
GYIDKDIEKIVGLQTDAPLKRGFFPEGGINVAVKSVEAYGYKVEQETIDIYTNVRKTHNQGVFDAYTKEIRNARSSHIIT
GLPDGYGRGRIIGDYRRVPLYGVDALIKDKYAQQEELTFPMSEHKIRLREELSDQIKSLNELIQLGDMYGFDLRRPASDS
KEAIQWLYLAYLAAVKEQNGAAMSLGRVDAFLDIYIERDLKAAKYSELEIQEFIDHFVMKLRMVRFARTPEYNQLFTGDP
TWVTAVLGGMSNDGRSLVTKTAFRLLQTLYNLGTSAEPNLTVLWSNNLPEGFKNFTAKVSIDTSSIQYENDDTIRPLHGD
DYGIACCVSPMAIGKEMQFFGARANIAKALLYALNGGRDEVSGKQVFEKSKMEVLKTDVLDYDVVMSRYEKTLEELAEVY
VNALNIIHYMHDKYAYEKLEMALHDRDVKRLFATGIAGLSVVADSLSAIKYAKVHPIYDETSGLITDFETVGEFPCYGND
DDRVDDIAVDVLRKFMSYIRKNPTYRESHPTMSVLTITSNVVYGKNTGNTPDGRRYGEPFAPGANPMHGRDKSGALKSLM
SVAKLPYEDAEDGISNTFTIIPKALGHSISAEGSIVHLVDDVQVNNLVSLLDGYFTSGGYHLNVNVFNRETLKAAYDNPE
LYPNLTIRVSGYAVNFSNLTNEQKLEVMKRTFHEGF
>Mature_755_residues
AQSWKGFKSGNWEKAVDVADFIKQNYSEYTGDGTFLEGAVESSLSLNESFKDLLKLEKEKGGVIDLDTKVASTIISHAPG
YIDKDIEKIVGLQTDAPLKRGFFPEGGINVAVKSVEAYGYKVEQETIDIYTNVRKTHNQGVFDAYTKEIRNARSSHIITG
LPDGYGRGRIIGDYRRVPLYGVDALIKDKYAQQEELTFPMSEHKIRLREELSDQIKSLNELIQLGDMYGFDLRRPASDSK
EAIQWLYLAYLAAVKEQNGAAMSLGRVDAFLDIYIERDLKAAKYSELEIQEFIDHFVMKLRMVRFARTPEYNQLFTGDPT
WVTAVLGGMSNDGRSLVTKTAFRLLQTLYNLGTSAEPNLTVLWSNNLPEGFKNFTAKVSIDTSSIQYENDDTIRPLHGDD
YGIACCVSPMAIGKEMQFFGARANIAKALLYALNGGRDEVSGKQVFEKSKMEVLKTDVLDYDVVMSRYEKTLEELAEVYV
NALNIIHYMHDKYAYEKLEMALHDRDVKRLFATGIAGLSVVADSLSAIKYAKVHPIYDETSGLITDFETVGEFPCYGNDD
DRVDDIAVDVLRKFMSYIRKNPTYRESHPTMSVLTITSNVVYGKNTGNTPDGRRYGEPFAPGANPMHGRDKSGALKSLMS
VAKLPYEDAEDGISNTFTIIPKALGHSISAEGSIVHLVDDVQVNNLVSLLDGYFTSGGYHLNVNVFNRETLKAAYDNPEL
YPNLTIRVSGYAVNFSNLTNEQKLEVMKRTFHEGF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=765, Percent_Identity=59.0849673202614, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=766, Percent_Identity=56.9190600522193, Blast_Score=877, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=592, Percent_Identity=24.4932432432432, Blast_Score=149, Evalue=8e-37,
Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=23.3986928104575, Blast_Score=148, Evalue=2e-36,
Organism=Escherichia coli, GI1788933, Length=59, Percent_Identity=62.7118644067797, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84722; Mature: 84591

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSWKGFKSGNWEKAVDVADFIKQNYSEYTGDGTFLEGAVESSLSLNESFKDLLKLEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHH
KGGVIDLDTKVASTIISHAPGYIDKDIEKIVGLQTDAPLKRGFFPEGGINVAVKSVEAYG
CCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEEHHHHC
YKVEQETIDIYTNVRKTHNQGVFDAYTKEIRNARSSHIITGLPDGYGRGRIIGDYRRVPL
EEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCH
YGVDALIKDKYAQQEELTFPMSEHKIRLREELSDQIKSLNELIQLGDMYGFDLRRPASDS
HHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
KEAIQWLYLAYLAAVKEQNGAAMSLGRVDAFLDIYIERDLKAAKYSELEIQEFIDHFVMK
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LRMVRFARTPEYNQLFTGDPTWVTAVLGGMSNDGRSLVTKTAFRLLQTLYNLGTSAEPNL
HHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
TVLWSNNLPEGFKNFTAKVSIDTSSIQYENDDTIRPLHGDDYGIACCVSPMAIGKEMQFF
EEEECCCCCCCHHCCEEEEEEECCEEEECCCCCCCCCCCCCCCEEEECCHHHHCCHHHHH
GARANIAKALLYALNGGRDEVSGKQVFEKSKMEVLKTDVLDYDVVMSRYEKTLEELAEVY
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNALNIIHYMHDKYAYEKLEMALHDRDVKRLFATGIAGLSVVADSLSAIKYAKVHPIYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
TSGLITDFETVGEFPCYGNDDDRVDDIAVDVLRKFMSYIRKNPTYRESHPTMSVLTITSN
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECC
VVYGKNTGNTPDGRRYGEPFAPGANPMHGRDKSGALKSLMSVAKLPYEDAEDGISNTFTI
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCHHHH
IPKALGHSISAEGSIVHLVDDVQVNNLVSLLDGYFTSGGYHLNVNVFNRETLKAAYDNPE
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHCCCCC
LYPNLTIRVSGYAVNFSNLTNEQKLEVMKRTFHEGF
CCCCEEEEEEEEEEEHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQSWKGFKSGNWEKAVDVADFIKQNYSEYTGDGTFLEGAVESSLSLNESFKDLLKLEKE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHH
KGGVIDLDTKVASTIISHAPGYIDKDIEKIVGLQTDAPLKRGFFPEGGINVAVKSVEAYG
CCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEEHHHHC
YKVEQETIDIYTNVRKTHNQGVFDAYTKEIRNARSSHIITGLPDGYGRGRIIGDYRRVPL
EEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCH
YGVDALIKDKYAQQEELTFPMSEHKIRLREELSDQIKSLNELIQLGDMYGFDLRRPASDS
HHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
KEAIQWLYLAYLAAVKEQNGAAMSLGRVDAFLDIYIERDLKAAKYSELEIQEFIDHFVMK
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LRMVRFARTPEYNQLFTGDPTWVTAVLGGMSNDGRSLVTKTAFRLLQTLYNLGTSAEPNL
HHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
TVLWSNNLPEGFKNFTAKVSIDTSSIQYENDDTIRPLHGDDYGIACCVSPMAIGKEMQFF
EEEECCCCCCCHHCCEEEEEEECCEEEECCCCCCCCCCCCCCCEEEECCHHHHCCHHHHH
GARANIAKALLYALNGGRDEVSGKQVFEKSKMEVLKTDVLDYDVVMSRYEKTLEELAEVY
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNALNIIHYMHDKYAYEKLEMALHDRDVKRLFATGIAGLSVVADSLSAIKYAKVHPIYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
TSGLITDFETVGEFPCYGNDDDRVDDIAVDVLRKFMSYIRKNPTYRESHPTMSVLTITSN
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECC
VVYGKNTGNTPDGRRYGEPFAPGANPMHGRDKSGALKSLMSVAKLPYEDAEDGISNTFTI
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCHHHH
IPKALGHSISAEGSIVHLVDDVQVNNLVSLLDGYFTSGGYHLNVNVFNRETLKAAYDNPE
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHCCCCC
LYPNLTIRVSGYAVNFSNLTNEQKLEVMKRTFHEGF
CCCCEEEEEEEEEEEHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA