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The map label for this gene is flp [H]

Identifier: 162446917

GI number: 162446917

Start: 35535

End: 36230

Strand: Direct

Name: flp [H]

Synonym: ACL_0030

Alternate gene names: 162446917

Gene position: 35535-36230 (Clockwise)

Preceding gene: 162446916

Following gene: 162446918

Centisome position: 2.37

GC content: 28.45

Gene sequence:

>696_bases
ATGTCTCATACACACAAAGATCATGACCATACATGCTTGGTCACGGTACCTATATTTAATCATCTAGAATTAGATGAACT
AACAAAGATCACAGAACTTATCAAATCTAAATCATTTGATAAAGGTGAACTTATTTTTAGTTATGGTGAAAAATCAGACA
CCTTATATATTGTAAGAAGTGGACAAATTAAAATGTACTACTTATCTGAAAATGCTAAAGAACATATTTTAAGAATGTTA
TTTCCAGGAGACTTTATAGGTGAGTTAAGTATTTTCTTAGATGAAAAAAACAATGCTTATGCAGAAGCAATAACTCCAGT
TGAAGTATGCATGATAAAAAAAGAAGACATCTCAAAGCTTATGGAGATATATCCTAAAATTGGTCTTAAAATTATTGGTG
AATTAACCCAGAGATTAAAGAAATCAGAAAAACAAGCAAGTTGGATTGCTACAGAACAAGTAGAAAAAAGAATCCTGTTA
TATATGCTTGAACTGTATGATTCAAATGAGCCATTAAAGCCAATTGAACTTCCAATGACTAAAAAAGATTTAGCATCCTT
TCTAGGTACTACACCAGAAACTTTAAGTAGAAAACTAGCTTACTTAGAAGATTTAGGTATGATACATCAATTAAGTAATA
AACAAATTAAAATTGTTAAAATGGATTTACTATTAGAATTTTTAAATCAAATGTAA

Upstream 100 bases:

>100_bases
TTACTTTATATGTTGTTTTCTAATATAACCTCTAAGGAAATTGAATGTGGAAAAACTTAATACCTTTTAGTGTATAATGG
ATATAGATTGGAGTGATTTT

Downstream 100 bases:

>100_bases
TAAAAACATAAGAACTAAAATTCTTATGTTTTTATGTATGAATTAAGTTAAGGAAGATATCATGACAATTATTTTTGCCC
CTACAAAACTATTTAACCCA

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSHTHKDHDHTCLVTVPIFNHLELDELTKITELIKSKSFDKGELIFSYGEKSDTLYIVRSGQIKMYYLSENAKEHILRML
FPGDFIGELSIFLDEKNNAYAEAITPVEVCMIKKEDISKLMEIYPKIGLKIIGELTQRLKKSEKQASWIATEQVEKRILL
YMLELYDSNEPLKPIELPMTKKDLASFLGTTPETLSRKLAYLEDLGMIHQLSNKQIKIVKMDLLLEFLNQM

Sequences:

>Translated_231_residues
MSHTHKDHDHTCLVTVPIFNHLELDELTKITELIKSKSFDKGELIFSYGEKSDTLYIVRSGQIKMYYLSENAKEHILRML
FPGDFIGELSIFLDEKNNAYAEAITPVEVCMIKKEDISKLMEIYPKIGLKIIGELTQRLKKSEKQASWIATEQVEKRILL
YMLELYDSNEPLKPIELPMTKKDLASFLGTTPETLSRKLAYLEDLGMIHQLSNKQIKIVKMDLLLEFLNQM
>Mature_230_residues
SHTHKDHDHTCLVTVPIFNHLELDELTKITELIKSKSFDKGELIFSYGEKSDTLYIVRSGQIKMYYLSENAKEHILRMLF
PGDFIGELSIFLDEKNNAYAEAITPVEVCMIKKEDISKLMEIYPKIGLKIIGELTQRLKKSEKQASWIATEQVEKRILLY
MLELYDSNEPLKPIELPMTKKDLASFLGTTPETLSRKLAYLEDLGMIHQLSNKQIKIVKMDLLLEFLNQM

Specific function: Global Transcription Factor That Controls The Expression Of Over 100 Target Genes In Response To Anoxia. It Facilitates The Adaptation To Anaerobic Growth Conditions By Regulating The Expression Of Gene Products That Are Involved In Anaerobic Energy Metab

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=228, Percent_Identity=27.6315789473684, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789756, Length=164, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26758; Mature: 26627

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTHKDHDHTCLVTVPIFNHLELDELTKITELIKSKSFDKGELIFSYGEKSDTLYIVRS
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEC
GQIKMYYLSENAKEHILRMLFPGDFIGELSIFLDEKNNAYAEAITPVEVCMIKKEDISKL
CCEEEEEECCCHHHHHHHHHCCCCHHHCEEEEEECCCCCHHHHCCHHHHEEECHHHHHHH
MEIYPKIGLKIIGELTQRLKKSEKQASWIATEQVEKRILLYMLELYDSNEPLKPIELPMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KKDLASFLGTTPETLSRKLAYLEDLGMIHQLSNKQIKIVKMDLLLEFLNQM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCC
>Mature Secondary Structure 
SHTHKDHDHTCLVTVPIFNHLELDELTKITELIKSKSFDKGELIFSYGEKSDTLYIVRS
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEC
GQIKMYYLSENAKEHILRMLFPGDFIGELSIFLDEKNNAYAEAITPVEVCMIKKEDISKL
CCEEEEEECCCHHHHHHHHHCCCCHHHCEEEEEECCCCCHHHHCCHHHHEEECHHHHHHH
MEIYPKIGLKIIGELTQRLKKSEKQASWIATEQVEKRILLYMLELYDSNEPLKPIELPMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KKDLASFLGTTPETLSRKLAYLEDLGMIHQLSNKQIKIVKMDLLLEFLNQM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]