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The map label for this gene is leuB [H]

Identifier: 162148856

GI number: 162148856

Start: 3180890

End: 3182002

Strand: Reverse

Name: leuB [H]

Synonym: GDI_3085

Alternate gene names: 162148856

Gene position: 3182002-3180890 (Counterclockwise)

Preceding gene: 162148857

Following gene: 162148854

Centisome position: 80.68

GC content: 66.76

Gene sequence:

>1113_bases
ATGACCGTTACCAAGAAGCTCCTTGTCCTGCCCGGAGACGGGATCGGCCCCGAGATCATGCACGAAGTCGCGCGGGTCGT
GGCGTGGATGAGCCGCACGCGCGGCATCGTCTTCGACATTTCCGAGGACCTGGTCGGTGGCGCGTCGCTGGCGGTGCATG
GCGTGCCGATTCGTGACGAGGTGATCGCGGCGGCGAAGGCGGCCGACGCGGTGCTGTTCGGTTCGGTCGGCGATCCGAAA
TGGGCGTCGGCCGGTTTCGACCGCCGCCCGGAAATCGCGATCCTGAAACTGCGCCAGGAACTGGGACTGTTCGCCAACCT
GCGTCCGGCCAAGGTGTTCGACGCGCTGGTCGATTCCAGCACGCTGAAGCCGGAGGTCGTGGCCGGGCTGGATATCATGA
TCGTGCGCGAAACCGTGGGCGGCATCTATTTCGGCGAGCCGCGCGGGATCGAGACCCTGCCCGACGGCACGAAGCGCGGC
ATCAACACGGAAGTCTACACCACGGCGGAAATCGAGCGCGTCGCCCGCGTCGCCTTCGACCTGGCGCGGCAGCGCGGCAA
TCGCGTCTGCTCGGTCGAAAAATGCAACGTGATGGAAAGCGGCCTGTTGTGGAAAGAGGTCGTGACCGACCTGCACGCGC
GCGAGTACGCGGATGTCGAACTCAGCCACATGCTGGCGGATAATTGCGCGATGCAGATGGTGCGCAACCCGCGCCAGTTC
GACGTGATCGTGACCGGCAACTTGTTCGGCGACCTGCTGTCCGACCTGGCGTCGATGCTGACCGGCAGCCTGGGCATGCT
GCCCTCCGCCACGCTGGGCGCGGTGGACGCCGCCGGCCGCCGGCCCGCGCTGTATGAACCCATCCACGGCAGCGCGCCCG
ACATCGCGGGCAAGGGCATCGCGAATCCGCTGGCGCAGATCCTCTCGTTCTCGATGCTGCTGCGCTATTCCTTCGCGATG
GAGGCCGAGGCCGCGCTGATCGAAAAGGCGGTCGCCAACGTCCTGGCCAGCGGCCTGCGCACCGCCGACATCATGAGCCC
CGGCATGGCCCCGGTCAGCACCGCTGTCATGGGCGAGGCCGTGGTGCGCGAACTCGACAAACTGAACGGCTGA

Upstream 100 bases:

>100_bases
GATCGACACTTTCGAGGCCACGCGCGAACGGGCCCAGCCCTGGCAGACGCGGATCGTCATCGACTGACGCCGCACCCGTC
GTCACTACTGGAAGACCGAG

Downstream 100 bases:

>100_bases
GGCTGTTCCCGCCGGGCCCGGACAGGGCCCGGCCTTTTGGGACAACAGATATGGAAAAGGCCGGCATCCCCAGGATGCCG
GCCTTTCTGATTCGGACTGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTVTKKLLVLPGDGIGPEIMHEVARVVAWMSRTRGIVFDISEDLVGGASLAVHGVPIRDEVIAAAKAADAVLFGSVGDPK
WASAGFDRRPEIAILKLRQELGLFANLRPAKVFDALVDSSTLKPEVVAGLDIMIVRETVGGIYFGEPRGIETLPDGTKRG
INTEVYTTAEIERVARVAFDLARQRGNRVCSVEKCNVMESGLLWKEVVTDLHAREYADVELSHMLADNCAMQMVRNPRQF
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAVDAAGRRPALYEPIHGSAPDIAGKGIANPLAQILSFSMLLRYSFAM
EAEAALIEKAVANVLASGLRTADIMSPGMAPVSTAVMGEAVVRELDKLNG

Sequences:

>Translated_370_residues
MTVTKKLLVLPGDGIGPEIMHEVARVVAWMSRTRGIVFDISEDLVGGASLAVHGVPIRDEVIAAAKAADAVLFGSVGDPK
WASAGFDRRPEIAILKLRQELGLFANLRPAKVFDALVDSSTLKPEVVAGLDIMIVRETVGGIYFGEPRGIETLPDGTKRG
INTEVYTTAEIERVARVAFDLARQRGNRVCSVEKCNVMESGLLWKEVVTDLHAREYADVELSHMLADNCAMQMVRNPRQF
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAVDAAGRRPALYEPIHGSAPDIAGKGIANPLAQILSFSMLLRYSFAM
EAEAALIEKAVANVLASGLRTADIMSPGMAPVSTAVMGEAVVRELDKLNG
>Mature_369_residues
TVTKKLLVLPGDGIGPEIMHEVARVVAWMSRTRGIVFDISEDLVGGASLAVHGVPIRDEVIAAAKAADAVLFGSVGDPKW
ASAGFDRRPEIAILKLRQELGLFANLRPAKVFDALVDSSTLKPEVVAGLDIMIVRETVGGIYFGEPRGIETLPDGTKRGI
NTEVYTTAEIERVARVAFDLARQRGNRVCSVEKCNVMESGLLWKEVVTDLHAREYADVELSHMLADNCAMQMVRNPRQFD
VIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAVDAAGRRPALYEPIHGSAPDIAGKGIANPLAQILSFSMLLRYSFAME
AEAALIEKAVANVLASGLRTADIMSPGMAPVSTAVMGEAVVRELDKLNG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=351, Percent_Identity=32.4786324786325, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI28178821, Length=336, Percent_Identity=29.4642857142857, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI28178816, Length=336, Percent_Identity=29.7619047619048, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4758582, Length=367, Percent_Identity=29.9727520435967, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=29.9694189602446, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=176, Percent_Identity=34.6590909090909, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=47.3972602739726, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1788101, Length=381, Percent_Identity=30.7086614173228, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787381, Length=314, Percent_Identity=26.4331210191083, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=355, Percent_Identity=32.6760563380282, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=30.4469273743017, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=29.9145299145299, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=350, Percent_Identity=30.5714285714286, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=41.8230563002681, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=346, Percent_Identity=32.6589595375723, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=369, Percent_Identity=34.1463414634146, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=339, Percent_Identity=28.9085545722714, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=33.5276967930029, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=33.5276967930029, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=31.2320916905444, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI161078635, Length=343, Percent_Identity=29.7376093294461, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078637, Length=343, Percent_Identity=29.7376093294461, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078633, Length=343, Percent_Identity=29.7376093294461, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24650122, Length=343, Percent_Identity=29.7376093294461, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078639, Length=342, Percent_Identity=29.2397660818713, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI281362242, Length=345, Percent_Identity=28.1159420289855, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648872, Length=345, Percent_Identity=28.1159420289855, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20130355, Length=346, Percent_Identity=23.6994219653179, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39509; Mature: 39378

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTKKLLVLPGDGIGPEIMHEVARVVAWMSRTRGIVFDISEDLVGGASLAVHGVPIRDE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCEEEECCCCHHH
VIAAAKAADAVLFGSVGDPKWASAGFDRRPEIAILKLRQELGLFANLRPAKVFDALVDSS
HHHHHHHHCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHCCC
TLKPEVVAGLDIMIVRETVGGIYFGEPRGIETLPDGTKRGINTEVYTTAEIERVARVAFD
CCCCHHHHCCEEEEEEHHHCCEEECCCCCCCCCCCCHHCCCCCEEEEHHHHHHHHHHHHH
LARQRGNRVCSVEKCNVMESGLLWKEVVTDLHAREYADVELSHMLADNCAMQMVRNPRQF
HHHHHCCCEEEHHHCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAVDAAGRRPALYEPIHGSAPDIAGKGI
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
ANPLAQILSFSMLLRYSFAMEAEAALIEKAVANVLASGLRTADIMSPGMAPVSTAVMGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
VVRELDKLNG
HHHHHHHCCC
>Mature Secondary Structure 
TVTKKLLVLPGDGIGPEIMHEVARVVAWMSRTRGIVFDISEDLVGGASLAVHGVPIRDE
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCEEEECCCCHHH
VIAAAKAADAVLFGSVGDPKWASAGFDRRPEIAILKLRQELGLFANLRPAKVFDALVDSS
HHHHHHHHCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHCCC
TLKPEVVAGLDIMIVRETVGGIYFGEPRGIETLPDGTKRGINTEVYTTAEIERVARVAFD
CCCCHHHHCCEEEEEEHHHCCEEECCCCCCCCCCCCHHCCCCCEEEEHHHHHHHHHHHHH
LARQRGNRVCSVEKCNVMESGLLWKEVVTDLHAREYADVELSHMLADNCAMQMVRNPRQF
HHHHHCCCEEEHHHCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
DVIVTGNLFGDLLSDLASMLTGSLGMLPSATLGAVDAAGRRPALYEPIHGSAPDIAGKGI
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
ANPLAQILSFSMLLRYSFAMEAEAALIEKAVANVLASGLRTADIMSPGMAPVSTAVMGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
VVRELDKLNG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA