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The map label for this gene is leuD

Identifier: 162148857

GI number: 162148857

Start: 3182036

End: 3182659

Strand: Reverse

Name: leuD

Synonym: GDI_3086

Alternate gene names: 162148857

Gene position: 3182659-3182036 (Counterclockwise)

Preceding gene: 162148858

Following gene: 162148856

Centisome position: 80.69

GC content: 64.58

Gene sequence:

>624_bases
ATGGAGAAATTCACCGTCCTGACCGCGATCGCGGCGCCGCTGCCCGAAGCGAACATCGACACGGACAAGATCATTCCCGC
CCGGTTCCTGAAGACCACCAAGCGGTCGGGGCTGGGCGTGCATGCCTTCGACGGCATGCGCTACAATCCGGACGGGTCCG
AAAAGCCCGATTTCGTGCTGAACCAGGCGCCCTATCGCCAGGCCGGCATCCTGATCACTTACGACAATCTCGGCTGCGGT
TCGTCGCGCGAACATGCGCCGTGGGCGCTGCTGGATTTCGGCATCCGCTGCGTGATCGCGCCGTCCTTTGCGGATATCTT
CTTCAATAACTGCTTCAAGAACGGAATCCTGCCGATCCGGCTGCCGCGCGAGGTCTGCGACGCGCTGATGGACGATGCGC
GGCTGGGCGGCAACGGCCGGCTGACGGTCGATCTGGAACGCCAGGTGGTCATCCGCCCGGACGGCGCCGAAATCGCCTTC
GAGATCGATCCGCTGCGCCGGCACCTGCTGCTGGAAGGGCTGGACGATATCGGCCAGACCCTGCAGCACGAAGGCGCGAT
CGACACTTTCGAGGCCACGCGCGAACGGGCCCAGCCCTGGCAGACGCGGATCGTCATCGACTGA

Upstream 100 bases:

>100_bases
GCAGGGCCCGGGCGGCCGCACCCACCTGCTGTCGCCCGCCATGGCGGCGGCGGCGGCGGTCACGGGACATCTGACAGACG
TGCGGGAGCTGATCTGAGCC

Downstream 100 bases:

>100_bases
CGCCGCACCCGTCGTCACTACTGGAAGACCGAGATGACCGTTACCAAGAAGCTCCTTGTCCTGCCCGGAGACGGGATCGG
CCCCGAGATCATGCACGAAG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MEKFTVLTAIAAPLPEANIDTDKIIPARFLKTTKRSGLGVHAFDGMRYNPDGSEKPDFVLNQAPYRQAGILITYDNLGCG
SSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIRLPREVCDALMDDARLGGNGRLTVDLERQVVIRPDGAEIAF
EIDPLRRHLLLEGLDDIGQTLQHEGAIDTFEATRERAQPWQTRIVID

Sequences:

>Translated_207_residues
MEKFTVLTAIAAPLPEANIDTDKIIPARFLKTTKRSGLGVHAFDGMRYNPDGSEKPDFVLNQAPYRQAGILITYDNLGCG
SSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIRLPREVCDALMDDARLGGNGRLTVDLERQVVIRPDGAEIAF
EIDPLRRHLLLEGLDDIGQTLQHEGAIDTFEATRERAQPWQTRIVID
>Mature_207_residues
MEKFTVLTAIAAPLPEANIDTDKIIPARFLKTTKRSGLGVHAFDGMRYNPDGSEKPDFVLNQAPYRQAGILITYDNLGCG
SSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIRLPREVCDALMDDARLGGNGRLTVDLERQVVIRPDGAEIAF
EIDPLRRHLLLEGLDDIGQTLQHEGAIDTFEATRERAQPWQTRIVID

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=46.6666666666667, Blast_Score=177, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321429, Length=201, Percent_Identity=53.2338308457711, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_GLUDA (A9HS54)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001603318.1
- RefSeq:   YP_002277623.1
- ProteinModelPortal:   A9HS54
- SMR:   A9HS54
- GeneID:   5791915
- GeneID:   6976720
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3281
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23088; Mature: 23088

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTVLTAIAAPLPEANIDTDKIIPARFLKTTKRSGLGVHAFDGMRYNPDGSEKPDFVL
CCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEE
NQAPYRQAGILITYDNLGCGSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHEECCEEEEECCHHHHHHHHHHHHCCCEEEE
LPREVCDALMDDARLGGNGRLTVDLERQVVIRPDGAEIAFEIDPLRRHLLLEGLDDIGQT
CCHHHHHHHHHHHEECCCCEEEEEECCEEEECCCCCEEEEEECHHHHHHHHHCHHHHHHH
LQHEGAIDTFEATRERAQPWQTRIVID
HHHCCCCCHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MEKFTVLTAIAAPLPEANIDTDKIIPARFLKTTKRSGLGVHAFDGMRYNPDGSEKPDFVL
CCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEE
NQAPYRQAGILITYDNLGCGSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHEECCEEEEECCHHHHHHHHHHHHCCCEEEE
LPREVCDALMDDARLGGNGRLTVDLERQVVIRPDGAEIAFEIDPLRRHLLLEGLDDIGQT
CCHHHHHHHHHHHEECCCCEEEEEECCEEEECCCCCEEEEEECHHHHHHHHHCHHHHHHH
LQHEGAIDTFEATRERAQPWQTRIVID
HHHCCCCCHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA