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The map label for this gene is def2 [H]

Identifier: 162148854

GI number: 162148854

Start: 3179824

End: 3180354

Strand: Reverse

Name: def2 [H]

Synonym: GDI_3083

Alternate gene names: 162148854

Gene position: 3180354-3179824 (Counterclockwise)

Preceding gene: 162148856

Following gene: 162148853

Centisome position: 80.63

GC content: 67.42

Gene sequence:

>531_bases
ATGACCCTGCTCAAGATTGCCCGGATGGGCCATCCCGTGCTGCTGCGCCGCGCGGACGAGGTCGCGGACCCCACCGCGCC
CGACATCGCGCGCCTGATCGACGACATGATCGAGACGATGGAGGACGCGCGCGGCGCGGGCCTGGCGGCGCCGCAGGTCC
ATGTCTCGCTGCGGCTGTTCGTCTACCGCGTGCCGGCCGAACGCAGCGCCGGAGGGGACGATCCGCCCCGTGAGACCAGC
GTGCTGATCAATCCCGTCCTCAGCCTCGTGGATGACGAGATGGCGTTGCGGCCCGAGGGCTGCCTGTCGATTCCCGGCCT
GCGCGGCATGGTGCCCCGGCATGTCCGCATCGCCTATTCCGGCCTGGACCGGGCAGGGCAGGCGGTGCAGGGCGTCGCGT
CGGGTTTCCTGGCGAACGTGCTACAGCATGAATACGATCACCTGGACGGTATTCTCTACCCGATGCGGATGACCGATCTG
GGGCAGATGGGATTCGACGAGGAAATCGGACGATACGGAGTACGCACATGA

Upstream 100 bases:

>100_bases
GGGCTTCGCGGAAAACACAAATCATTCCGCCCGTTTCCCGCAGGAAGCGGCCAGACTATTTTAGAGGCCCTGCTTTACCG
GCGAAGGACGGCTACAGACC

Downstream 100 bases:

>100_bases
TCGTGAAGCGATGCGCCAGCGGCCTGGCGGCCCGGCTGGCAGGATGCGCCGCCGCCTGCGCCCTTCCGGGGCGGCCGGAA
CGCAGCGCGGCGCGTGACGC

Product: peptide deformylase 2

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MTLLKIARMGHPVLLRRADEVADPTAPDIARLIDDMIETMEDARGAGLAAPQVHVSLRLFVYRVPAERSAGGDDPPRETS
VLINPVLSLVDDEMALRPEGCLSIPGLRGMVPRHVRIAYSGLDRAGQAVQGVASGFLANVLQHEYDHLDGILYPMRMTDL
GQMGFDEEIGRYGVRT

Sequences:

>Translated_176_residues
MTLLKIARMGHPVLLRRADEVADPTAPDIARLIDDMIETMEDARGAGLAAPQVHVSLRLFVYRVPAERSAGGDDPPRETS
VLINPVLSLVDDEMALRPEGCLSIPGLRGMVPRHVRIAYSGLDRAGQAVQGVASGFLANVLQHEYDHLDGILYPMRMTDL
GQMGFDEEIGRYGVRT
>Mature_175_residues
TLLKIARMGHPVLLRRADEVADPTAPDIARLIDDMIETMEDARGAGLAAPQVHVSLRLFVYRVPAERSAGGDDPPRETSV
LINPVLSLVDDEMALRPEGCLSIPGLRGMVPRHVRIAYSGLDRAGQAVQGVASGFLANVLQHEYDHLDGILYPMRMTDLG
QMGFDEEIGRYGVRT

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=33.75, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1789682, Length=157, Percent_Identity=36.9426751592357, Blast_Score=91, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24645728, Length=162, Percent_Identity=32.0987654320988, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=31.6455696202532, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19243; Mature: 19112

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLKIARMGHPVLLRRADEVADPTAPDIARLIDDMIETMEDARGAGLAAPQVHVSLRLF
CCHHHHHHCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEE
VYRVPAERSAGGDDPPRETSVLINPVLSLVDDEMALRPEGCLSIPGLRGMVPRHVRIAYS
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
GLDRAGQAVQGVASGFLANVLQHEYDHLDGILYPMRMTDLGQMGFDEEIGRYGVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure 
TLLKIARMGHPVLLRRADEVADPTAPDIARLIDDMIETMEDARGAGLAAPQVHVSLRLF
CHHHHHHCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEE
VYRVPAERSAGGDDPPRETSVLINPVLSLVDDEMALRPEGCLSIPGLRGMVPRHVRIAYS
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
GLDRAGQAVQGVASGFLANVLQHEYDHLDGILYPMRMTDLGQMGFDEEIGRYGVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]