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The map label for this gene is glyA [H]

Identifier: 162148783

GI number: 162148783

Start: 3098376

End: 3099698

Strand: Reverse

Name: glyA [H]

Synonym: GDI_3012

Alternate gene names: 162148783

Gene position: 3099698-3098376 (Counterclockwise)

Preceding gene: 162148787

Following gene: 162148782

Centisome position: 78.59

GC content: 68.56

Gene sequence:

>1323_bases
TTGAAGGAACTGGAGAATATAACGATGCCGGACCAGATGAGCCAGAGCGGGTTGCACGCCTATTTCCGCTCCCCGCTTGC
CGAACGCGATCCCCTGGTCGCGGAGATCATCGCGGGTGAACTGGAACGCCAGCGCGACGGAATCGAACTGATCGCCAGCG
AGAACATGGTGTCCGAGGCGGTGCTGCAGGCGCAGGGCAGCGTGCTGACGAACAAATACGCCGAGGGTTATCCCGGCCGC
CGCTATTACGGCGGCTGCGCCGAGGTGGACAAGGTCGAGAGCCTGGCCATCGAGCGGGTGAAGACGCTGTTCGGCGCGGG
CTTCGCGAACGTGCAGCCCCATTCCGGCGCCAACGCGAACCAGGCGGCGTTCATGGCGCTGGTCAGCCCGGGCGATACCA
TTCTGGGCATGAGCCTGGCGGCGGGCGGCCACCTGACGCACGGGGCGGCGCCGAACTATTCCGGCAAATGGTTCCGCGCG
GTGCAGTACGGCGTGCGGCGCGAGGACGGGCTGCTGGATTACGAGGAGATGGAGCGCCTGGCCCGGGCCGAGAAGCCGAA
GCTGATCGTGGCGGGGGGCTCGGCCTATCCGCGCGCGATCGATTTCGCCCGCTTCCGCGCCATCGCGGACGAAGTCGGGG
CCTACCTGATGGTCGACATGGCCCATTATGCCGGACTGGTTGCGGCGGGCCTGTATCCCTCGCCGATGGCGCATGCGCAT
GTGGTGACCAGCACGACGCACAAGACCCTGCGCGGCCCGCGCGGCGGCCTGATCCTGACGAATGACGCGGACCTGGCGAA
GAAGATCAACTCGGCGGTCTTCCCCGGGCTGCAGGGCGGCCCGCTGATGCACGTGATCGCGGCCAAGGCCGTGGCGTTCG
GCGAGGCGCTGCAGCCGGAATTCCGCGCCTATCAGGAAGCGGTGGCGGCGAATGCCCGCGTGCTGGCGGAAACGCTGCTG
TCGCGCGGGTTCGACATCGTGACGGGGGGCACGGACAGCCACCTGCTGCTGGTGGACCTGCGCCCCAAGAAGGTCACGGG
CCGCGCCGCCGAACGCAGCCTGGAACGCGCCGGGATCACCGCGAACAAGAACGCGGTGCCGTTCGACCCGGAAAAGCCGG
CGATCACGTCGGGGATTCGCCTGGGCAGCCCCGCCGCCACGGCGCGCGGCTTCGGCACCGACGAATTCCGCGCGGTGGGC
GAGATGATCGACGAGGTCCTGACCGCCATGGCCGGCAAGGGCGAGGACGGATGCCCCGCCACCGAACAGGCGGTGCACGA
CAAGGTCCGCGCCCTGTGCGCGCGCTTCCCGATCTATCGCTAG

Upstream 100 bases:

>100_bases
CGTTGTGTGGCCTCGCCGACGTGGAGACGCTAGAATGCGCGAAATCACATTGACGCATGTGCAAGGGCCATTGGAACTCT
TCCGGTGACTGGCTGCGGCG

Downstream 100 bases:

>100_bases
GGTCTGCAGGCTCTCAAGCACGCGTAATCAGGTGACAACACCTGATTACGCGAAAGTATTTCGGGGGGATGAGTCATTTT
TAAGCCAGACCTGAGCTTCG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKELENITMPDQMSQSGLHAYFRSPLAERDPLVAEIIAGELERQRDGIELIASENMVSEAVLQAQGSVLTNKYAEGYPGR
RYYGGCAEVDKVESLAIERVKTLFGAGFANVQPHSGANANQAAFMALVSPGDTILGMSLAAGGHLTHGAAPNYSGKWFRA
VQYGVRREDGLLDYEEMERLARAEKPKLIVAGGSAYPRAIDFARFRAIADEVGAYLMVDMAHYAGLVAAGLYPSPMAHAH
VVTSTTHKTLRGPRGGLILTNDADLAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPEFRAYQEAVAANARVLAETLL
SRGFDIVTGGTDSHLLLVDLRPKKVTGRAAERSLERAGITANKNAVPFDPEKPAITSGIRLGSPAATARGFGTDEFRAVG
EMIDEVLTAMAGKGEDGCPATEQAVHDKVRALCARFPIYR

Sequences:

>Translated_440_residues
MKELENITMPDQMSQSGLHAYFRSPLAERDPLVAEIIAGELERQRDGIELIASENMVSEAVLQAQGSVLTNKYAEGYPGR
RYYGGCAEVDKVESLAIERVKTLFGAGFANVQPHSGANANQAAFMALVSPGDTILGMSLAAGGHLTHGAAPNYSGKWFRA
VQYGVRREDGLLDYEEMERLARAEKPKLIVAGGSAYPRAIDFARFRAIADEVGAYLMVDMAHYAGLVAAGLYPSPMAHAH
VVTSTTHKTLRGPRGGLILTNDADLAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPEFRAYQEAVAANARVLAETLL
SRGFDIVTGGTDSHLLLVDLRPKKVTGRAAERSLERAGITANKNAVPFDPEKPAITSGIRLGSPAATARGFGTDEFRAVG
EMIDEVLTAMAGKGEDGCPATEQAVHDKVRALCARFPIYR
>Mature_440_residues
MKELENITMPDQMSQSGLHAYFRSPLAERDPLVAEIIAGELERQRDGIELIASENMVSEAVLQAQGSVLTNKYAEGYPGR
RYYGGCAEVDKVESLAIERVKTLFGAGFANVQPHSGANANQAAFMALVSPGDTILGMSLAAGGHLTHGAAPNYSGKWFRA
VQYGVRREDGLLDYEEMERLARAEKPKLIVAGGSAYPRAIDFARFRAIADEVGAYLMVDMAHYAGLVAAGLYPSPMAHAH
VVTSTTHKTLRGPRGGLILTNDADLAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPEFRAYQEAVAANARVLAETLL
SRGFDIVTGGTDSHLLLVDLRPKKVTGRAAERSLERAGITANKNAVPFDPEKPAITSGIRLGSPAATARGFGTDEFRAVG
EMIDEVLTAMAGKGEDGCPATEQAVHDKVRALCARFPIYR

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=404, Percent_Identity=49.2574257425743, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862352, Length=404, Percent_Identity=49.2574257425743, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=404, Percent_Identity=49.2574257425743, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=404, Percent_Identity=49.2574257425743, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=48.2673267326733, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI22547186, Length=403, Percent_Identity=48.6352357320099, Blast_Score=335, Evalue=7e-92,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=46.0154241645244, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=56.25, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=49.5, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=49.5, Blast_Score=351, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=45.3883495145631, Blast_Score=355, Evalue=8e-99,
Organism=Saccharomyces cerevisiae, GI6323087, Length=422, Percent_Identity=42.8909952606635, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640005, Length=409, Percent_Identity=48.6552567237164, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221329721, Length=409, Percent_Identity=48.6552567237164, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 47006; Mature: 47006

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELENITMPDQMSQSGLHAYFRSPLAERDPLVAEIIAGELERQRDGIELIASENMVSEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
VLQAQGSVLTNKYAEGYPGRRYYGGCAEVDKVESLAIERVKTLFGAGFANVQPHSGANAN
HHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
QAAFMALVSPGDTILGMSLAAGGHLTHGAAPNYSGKWFRAVQYGVRREDGLLDYEEMERL
CEEEEEEECCCCCEEEEHECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
ARAEKPKLIVAGGSAYPRAIDFARFRAIADEVGAYLMVDMAHYAGLVAAGLYPSPMAHAH
HHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VVTSTTHKTLRGPRGGLILTNDADLAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPE
EEECCHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHH
FRAYQEAVAANARVLAETLLSRGFDIVTGGTDSHLLLVDLRPKKVTGRAAERSLERAGIT
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
ANKNAVPFDPEKPAITSGIRLGSPAATARGFGTDEFRAVGEMIDEVLTAMAGKGEDGCPA
CCCCCCCCCCCCCCHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
TEQAVHDKVRALCARFPIYR
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKELENITMPDQMSQSGLHAYFRSPLAERDPLVAEIIAGELERQRDGIELIASENMVSEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
VLQAQGSVLTNKYAEGYPGRRYYGGCAEVDKVESLAIERVKTLFGAGFANVQPHSGANAN
HHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
QAAFMALVSPGDTILGMSLAAGGHLTHGAAPNYSGKWFRAVQYGVRREDGLLDYEEMERL
CEEEEEEECCCCCEEEEHECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
ARAEKPKLIVAGGSAYPRAIDFARFRAIADEVGAYLMVDMAHYAGLVAAGLYPSPMAHAH
HHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VVTSTTHKTLRGPRGGLILTNDADLAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPE
EEECCHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHH
FRAYQEAVAANARVLAETLLSRGFDIVTGGTDSHLLLVDLRPKKVTGRAAERSLERAGIT
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
ANKNAVPFDPEKPAITSGIRLGSPAATARGFGTDEFRAVGEMIDEVLTAMAGKGEDGCPA
CCCCCCCCCCCCCCHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
TEQAVHDKVRALCARFPIYR
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA