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The map label for this gene is ntrC [H]

Identifier: 162148049

GI number: 162148049

Start: 2324077

End: 2325525

Strand: Reverse

Name: ntrC [H]

Synonym: GDI_2265

Alternate gene names: 162148049

Gene position: 2325525-2324077 (Counterclockwise)

Preceding gene: 162148050

Following gene: 162148048

Centisome position: 58.96

GC content: 69.63

Gene sequence:

>1449_bases
ATGCCCCTGCCGACCGTCCTTGTCGCCGACGACGACCGTTCCATCCGCACCGTCCTTAGCCAGGCGCTGGGGCGCGCGGG
CTATCAGGTGCGCGTCACCGCCCATGCCGCGACCCTGTGGCAATGGGTCGAGGAGGGCGAGGGCGACCTGGTCATCACCG
ACGTGGTGATGCCCGACGAAAACGGCCTTGACCTGATTCCGCGCATCAAGCGGGCCCGGCCCGACCTGCGCGTGGTGGTC
ATGAGCGCGCAATCCACCCTGATGACCGCCGTCAAGGCGACCCAGCGCGGCGCGTTCGAGTACCTGCCCAAGCCCTTCGA
CCTGAAGGAGCTGCTGTCGGTGGTCGCGCGCGCGCTGGCGTCGCCGGCCGGCGCCCTGGCCGAACCCGCGCCGGTCGATA
CGGACGAACGCATGCCGCTGATCGGCCGGTCGATGGTGATGCAGGACATCTATCGCATCATCGCGCGACTGACGACGTCG
GACCTGACGGTGATGATCAATGGCGAGAGCGGCACCGGAAAGGAACTGGTGGCGCGCGCCCTGCACGATTACGGCCGCCG
GCGCGGCGGGCCCTTCGTCGCCGTCAACATGGCGGCGATCCCGCGCGAACTGATCGAGAGCGAGCTGTTCGGCCATGAGC
GCGGCGCGTTCACCGGGGCCACCAGCCGCAATCCCGGCCGCTTCGAACAGGCCGCCGGGGGCACCCTGTTCCTGGACGAG
ATCGGGGACATGCCGCCCGAGGCCCAGACGCGCCTGCTGCGCGTGCTGCAGGACGGCGAGTTCACCACCGTGGGCGGCCG
CCAGCCGATCCGCGCGAATGTCCGCATCATCGCCGCCACCCATCGCGACCTGCGCCAGGCGATCCGCACCGGATCGTTCC
GCGAGGACCTGTTCTATCGCCTGAACGTGGTGCCCATCCGCCTGCCGCCCCTGCGCGAGCGGGCCGAGGACATCCCCCTG
CTGGCCCGCCACTTCCTGGACCGCTGCCGCGAGGACGGCCTGCCGGGCAAGATTCTGGACGACGGCGCGGTGGAACGGCT
CCAGGCCAGCCGCTGGCCGGGCAATGTGCGCGAACTGGAAAACCTGATGCGCCGGCTGGCGGCCCTGTACCCGCAGGAGA
CGATCACCGCCGACCTGATCGATTCCGAACTGGCCGAAACGCTGCACGAGCAGGGCGCCGATACGCCGGACCTGCGCGAG
CAGGAACCGCTGGCCGACGCCGTGGCCCGCCATATCCGCCATTTCCTGGCCTCGGGCCAGGACGGCATGCCGCTGCACGA
CATCTATGACCGCGTGATCGCCGAGGTCGAACGGCCGCTGATCCAGATGACCCTGGCGGCCACCCGCGGCAACCAGATCA
AGGCGGCGGCGATGCTGGGGCTGAACCGCAACACCCTGCGCAAGAAGATCCGCGACCTGGAAATCCCTGTCGTTCGCGGC
GCGAACTGA

Upstream 100 bases:

>100_bases
CTGCACCTGCCCATGGTGGCCGAAGGCAGGACCCTTCCCTGACCGCCGGCCGGACGCCGCCCCCAGCCCTTAGCCCCGAT
TCAAGAGACTGACCTATCGC

Downstream 100 bases:

>100_bases
CCGTGAAGCGGGAGATCGTCCATTGACCGAACCCAGGGCATCCCGCGCCCGGATTCCGGCGCCGCCGCTGATGCGCGGCC
TGTTCGGGCTGCTGGCGCGG

Product: nitrogen assimilation regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPLPTVLVADDDRSIRTVLSQALGRAGYQVRVTAHAATLWQWVEEGEGDLVITDVVMPDENGLDLIPRIKRARPDLRVVV
MSAQSTLMTAVKATQRGAFEYLPKPFDLKELLSVVARALASPAGALAEPAPVDTDERMPLIGRSMVMQDIYRIIARLTTS
DLTVMINGESGTGKELVARALHDYGRRRGGPFVAVNMAAIPRELIESELFGHERGAFTGATSRNPGRFEQAAGGTLFLDE
IGDMPPEAQTRLLRVLQDGEFTTVGGRQPIRANVRIIAATHRDLRQAIRTGSFREDLFYRLNVVPIRLPPLRERAEDIPL
LARHFLDRCREDGLPGKILDDGAVERLQASRWPGNVRELENLMRRLAALYPQETITADLIDSELAETLHEQGADTPDLRE
QEPLADAVARHIRHFLASGQDGMPLHDIYDRVIAEVERPLIQMTLAATRGNQIKAAAMLGLNRNTLRKKIRDLEIPVVRG
AN

Sequences:

>Translated_482_residues
MPLPTVLVADDDRSIRTVLSQALGRAGYQVRVTAHAATLWQWVEEGEGDLVITDVVMPDENGLDLIPRIKRARPDLRVVV
MSAQSTLMTAVKATQRGAFEYLPKPFDLKELLSVVARALASPAGALAEPAPVDTDERMPLIGRSMVMQDIYRIIARLTTS
DLTVMINGESGTGKELVARALHDYGRRRGGPFVAVNMAAIPRELIESELFGHERGAFTGATSRNPGRFEQAAGGTLFLDE
IGDMPPEAQTRLLRVLQDGEFTTVGGRQPIRANVRIIAATHRDLRQAIRTGSFREDLFYRLNVVPIRLPPLRERAEDIPL
LARHFLDRCREDGLPGKILDDGAVERLQASRWPGNVRELENLMRRLAALYPQETITADLIDSELAETLHEQGADTPDLRE
QEPLADAVARHIRHFLASGQDGMPLHDIYDRVIAEVERPLIQMTLAATRGNQIKAAAMLGLNRNTLRKKIRDLEIPVVRG
AN
>Mature_481_residues
PLPTVLVADDDRSIRTVLSQALGRAGYQVRVTAHAATLWQWVEEGEGDLVITDVVMPDENGLDLIPRIKRARPDLRVVVM
SAQSTLMTAVKATQRGAFEYLPKPFDLKELLSVVARALASPAGALAEPAPVDTDERMPLIGRSMVMQDIYRIIARLTTSD
LTVMINGESGTGKELVARALHDYGRRRGGPFVAVNMAAIPRELIESELFGHERGAFTGATSRNPGRFEQAAGGTLFLDEI
GDMPPEAQTRLLRVLQDGEFTTVGGRQPIRANVRIIAATHRDLRQAIRTGSFREDLFYRLNVVPIRLPPLRERAEDIPLL
ARHFLDRCREDGLPGKILDDGAVERLQASRWPGNVRELENLMRRLAALYPQETITADLIDSELAETLHEQGADTPDLREQ
EPLADAVARHIRHFLASGQDGMPLHDIYDRVIAEVERPLIQMTLAATRGNQIKAAAMLGLNRNTLRKKIRDLEIPVVRGA
N

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=47.3684210526316, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=42.274678111588, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=37.4736842105263, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1788905, Length=382, Percent_Identity=42.6701570680628, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI87082117, Length=262, Percent_Identity=50.381679389313, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1789233, Length=342, Percent_Identity=44.4444444444444, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=42.9824561403509, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082152, Length=240, Percent_Identity=48.75, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87081872, Length=341, Percent_Identity=40.4692082111437, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786524, Length=278, Percent_Identity=46.0431654676259, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=41.4096916299559, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=34.3220338983051, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI87081858, Length=319, Percent_Identity=31.6614420062696, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789661, Length=89, Percent_Identity=41.5730337078652, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=140, Percent_Identity=27.8571428571429, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=28.9256198347107, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=33.9622641509434, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53449; Mature: 53318

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPTVLVADDDRSIRTVLSQALGRAGYQVRVTAHAATLWQWVEEGEGDLVITDVVMPDE
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEEECCC
NGLDLIPRIKRARPDLRVVVMSAQSTLMTAVKATQRGAFEYLPKPFDLKELLSVVARALA
CCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHC
SPAGALAEPAPVDTDERMPLIGRSMVMQDIYRIIARLTTSDLTVMINGESGTGKELVARA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHDYGRRRGGPFVAVNMAAIPRELIESELFGHERGAFTGATSRNPGRFEQAAGGTLFLDE
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEHHH
IGDMPPEAQTRLLRVLQDGEFTTVGGRQPIRANVRIIAATHRDLRQAIRTGSFREDLFYR
HCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHHHEE
LNVVPIRLPPLRERAEDIPLLARHFLDRCREDGLPGKILDDGAVERLQASRWPGNVRELE
EEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
NLMRRLAALYPQETITADLIDSELAETLHEQGADTPDLREQEPLADAVARHIRHFLASGQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DGMPLHDIYDRVIAEVERPLIQMTLAATRGNQIKAAAMLGLNRNTLRKKIRDLEIPVVRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHCCCCEECC
AN
CC
>Mature Secondary Structure 
PLPTVLVADDDRSIRTVLSQALGRAGYQVRVTAHAATLWQWVEEGEGDLVITDVVMPDE
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEEECCC
NGLDLIPRIKRARPDLRVVVMSAQSTLMTAVKATQRGAFEYLPKPFDLKELLSVVARALA
CCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHC
SPAGALAEPAPVDTDERMPLIGRSMVMQDIYRIIARLTTSDLTVMINGESGTGKELVARA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHDYGRRRGGPFVAVNMAAIPRELIESELFGHERGAFTGATSRNPGRFEQAAGGTLFLDE
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEHHH
IGDMPPEAQTRLLRVLQDGEFTTVGGRQPIRANVRIIAATHRDLRQAIRTGSFREDLFYR
HCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHHHEE
LNVVPIRLPPLRERAEDIPLLARHFLDRCREDGLPGKILDDGAVERLQASRWPGNVRELE
EEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
NLMRRLAALYPQETITADLIDSELAETLHEQGADTPDLREQEPLADAVARHIRHFLASGQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DGMPLHDIYDRVIAEVERPLIQMTLAATRGNQIKAAAMLGLNRNTLRKKIRDLEIPVVRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHCCCCEECC
AN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]