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The map label for this gene is ntrB [C]

Identifier: 162148050

GI number: 162148050

Start: 2325584

End: 2326693

Strand: Reverse

Name: ntrB [C]

Synonym: GDI_2266

Alternate gene names: 162148050

Gene position: 2326693-2325584 (Counterclockwise)

Preceding gene: 162148051

Following gene: 162148049

Centisome position: 58.99

GC content: 67.93

Gene sequence:

>1110_bases
ATGGCGGCCGGCATGGCCCTCGACATGCCGCAGCCGGATGGCGCCGCGCTGCTGGACGCGATGTCGATGCCGGTGGTCCT
GGTGGGGCCGGGCAATCATATCCGCTACATCAACAGCGCGGCCGAACCGTTCTTCGGCGCGTCGCGCCAGCAACTGATGC
ATCGGGACCTGGCGGAAATCCTGGCGGCCGACCATCCGATCTTCCTGCTGGTCGAACAGGTGCGGCGCGACGGGGTGACG
ACCGCCGAACATGAACTGACGCTGGACGGGCCGCGGCTGCACCGCACGGGCATTACCGTGCAGGGCACGCAACTGCCCGA
TGAGCCGGATTCCGTGCTGCTGACCTTTCACGATTCGTCGGCGGCGCGGCAACTCGACCGGCAACTGACCTTCCGGTCCG
CGGCGCGCAGCGTGTCCGGCATGGCCGCCATCCTGGCGCACGAGGTCAAGAATCCCCTGTCCGGCATCAGGGGGGCGGCG
CAGTTGCTGGAAAGCTCGGTGGCCGAGGGCGACCGGGAACTGGCGGTGCTGATCCGGGACGAGGCCGACCGGATACGCGA
CCTGGTCGAGCGCATGGAAATGTTCAGCGACAAGCCGCTGGAACGGCGCCCGGTGAACATTCATCGCGTGCTCGAACGCG
TGCGCATGCTGGCCGAGCAGGGATTCGCCGGCAACATCCGGATCGTCGAGGATTACGATCCCTCGCTGCCGCCGGTCTGG
GGCAATCGCGACCAGTTGGTGCAGGTGCTGCTGAACCTGGTGAAGAACGCGGCCGAGGCCATCGCATCGGACGGGCAGGG
GGGGGAAATCACCCTCGGCACGTCCTACCGGCACGGGGTGCGGCTGGCGGTGCCGGGGATGGAACATCGCGTCGAACTGC
CGCTGGTGGTGTCGGTCCGCGATACCGGGCCCGGCATTCCCGACAGCATCCGGCCCCATCTGTTCGAACCCTTCCTGACG
ACGAAGACCAGCGGAAGCGGCCTGGGACTGGCCCTGGCGGGCAAGATCATCGACGACCATGGCGGCGTGATCGAAATCGA
CAGCCGCCCCGGCCGCACCGAGGTCCGCCTGCACCTGCCCATGGTGGCCGAAGGCAGGACCCTTCCCTGA

Upstream 100 bases:

>100_bases
TCTCCGCCTTCTATGACCGGCACATCGCTGCCGGTTCCCACCGCGCGCGGCGCGGCACGGCGGCGGGCGGTCAGGATGCC
GCCCAGGCGGCTTGAAACCC

Downstream 100 bases:

>100_bases
CCGCCGGCCGGACGCCGCCCCCAGCCCTTAGCCCCGATTCAAGAGACTGACCTATCGCATGCCCCTGCCGACCGTCCTTG
TCGCCGACGACGACCGTTCC

Product: histidine kinase, nitrogen regulation protein ntrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAAGMALDMPQPDGAALLDAMSMPVVLVGPGNHIRYINSAAEPFFGASRQQLMHRDLAEILAADHPIFLLVEQVRRDGVT
TAEHELTLDGPRLHRTGITVQGTQLPDEPDSVLLTFHDSSAARQLDRQLTFRSAARSVSGMAAILAHEVKNPLSGIRGAA
QLLESSVAEGDRELAVLIRDEADRIRDLVERMEMFSDKPLERRPVNIHRVLERVRMLAEQGFAGNIRIVEDYDPSLPPVW
GNRDQLVQVLLNLVKNAAEAIASDGQGGEITLGTSYRHGVRLAVPGMEHRVELPLVVSVRDTGPGIPDSIRPHLFEPFLT
TKTSGSGLGLALAGKIIDDHGGVIEIDSRPGRTEVRLHLPMVAEGRTLP

Sequences:

>Translated_369_residues
MAAGMALDMPQPDGAALLDAMSMPVVLVGPGNHIRYINSAAEPFFGASRQQLMHRDLAEILAADHPIFLLVEQVRRDGVT
TAEHELTLDGPRLHRTGITVQGTQLPDEPDSVLLTFHDSSAARQLDRQLTFRSAARSVSGMAAILAHEVKNPLSGIRGAA
QLLESSVAEGDRELAVLIRDEADRIRDLVERMEMFSDKPLERRPVNIHRVLERVRMLAEQGFAGNIRIVEDYDPSLPPVW
GNRDQLVQVLLNLVKNAAEAIASDGQGGEITLGTSYRHGVRLAVPGMEHRVELPLVVSVRDTGPGIPDSIRPHLFEPFLT
TKTSGSGLGLALAGKIIDDHGGVIEIDSRPGRTEVRLHLPMVAEGRTLP
>Mature_368_residues
AAGMALDMPQPDGAALLDAMSMPVVLVGPGNHIRYINSAAEPFFGASRQQLMHRDLAEILAADHPIFLLVEQVRRDGVTT
AEHELTLDGPRLHRTGITVQGTQLPDEPDSVLLTFHDSSAARQLDRQLTFRSAARSVSGMAAILAHEVKNPLSGIRGAAQ
LLESSVAEGDRELAVLIRDEADRIRDLVERMEMFSDKPLERRPVNIHRVLERVRMLAEQGFAGNIRIVEDYDPSLPPVWG
NRDQLVQVLLNLVKNAAEAIASDGQGGEITLGTSYRHGVRLAVPGMEHRVELPLVVSVRDTGPGIPDSIRPHLFEPFLTT
KTSGSGLGLALAGKIIDDHGGVIEIDSRPGRTEVRLHLPMVAEGRTLP

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=354, Percent_Identity=36.1581920903955, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788549, Length=285, Percent_Identity=29.4736842105263, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=33.0396475770925, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790567, Length=360, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=375, Percent_Identity=25.6, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=235, Percent_Identity=27.2340425531915, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=26.2931034482759, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40162; Mature: 40030

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGMALDMPQPDGAALLDAMSMPVVLVGPGNHIRYINSAAEPFFGASRQQLMHRDLAEI
CCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
LAADHPIFLLVEQVRRDGVTTAEHELTLDGPRLHRTGITVQGTQLPDEPDSVLLTFHDSS
HHCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCEEECCCCCCCCCCEEEEEECCH
AARQLDRQLTFRSAARSVSGMAAILAHEVKNPLSGIRGAAQLLESSVAEGDRELAVLIRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
EADRIRDLVERMEMFSDKPLERRPVNIHRVLERVRMLAEQGFAGNIRIVEDYDPSLPPVW
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
GNRDQLVQVLLNLVKNAAEAIASDGQGGEITLGTSYRHGVRLAVPGMEHRVELPLVVSVR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCEEECEEEEEEE
DTGPGIPDSIRPHLFEPFLTTKTSGSGLGLALAGKIIDDHGGVIEIDSRPGRTEVRLHLP
CCCCCCCCCCCCHHHCCHHHCCCCCCCCEEEEECEEEECCCCEEEECCCCCCEEEEEECC
MVAEGRTLP
EECCCCCCC
>Mature Secondary Structure 
AAGMALDMPQPDGAALLDAMSMPVVLVGPGNHIRYINSAAEPFFGASRQQLMHRDLAEI
CCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
LAADHPIFLLVEQVRRDGVTTAEHELTLDGPRLHRTGITVQGTQLPDEPDSVLLTFHDSS
HHCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCEEECCCCCCCCCCEEEEEECCH
AARQLDRQLTFRSAARSVSGMAAILAHEVKNPLSGIRGAAQLLESSVAEGDRELAVLIRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
EADRIRDLVERMEMFSDKPLERRPVNIHRVLERVRMLAEQGFAGNIRIVEDYDPSLPPVW
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
GNRDQLVQVLLNLVKNAAEAIASDGQGGEITLGTSYRHGVRLAVPGMEHRVELPLVVSVR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCEEECEEEEEEE
DTGPGIPDSIRPHLFEPFLTTKTSGSGLGLALAGKIIDDHGGVIEIDSRPGRTEVRLHLP
CCCCCCCCCCCCHHHCCHHHCCCCCCCCEEEEECEEEECCCCEEEECCCCCCEEEEEECC
MVAEGRTLP
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]