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The map label for this gene is fmt

Identifier: 162147912

GI number: 162147912

Start: 2182895

End: 2183812

Strand: Direct

Name: fmt

Synonym: GDI_2128

Alternate gene names: 162147912

Gene position: 2182895-2183812 (Clockwise)

Preceding gene: 162147911

Following gene: 162147913

Centisome position: 55.34

GC content: 74.18

Gene sequence:

>918_bases
ATGCGCCTGGCCTTCATGGGAACACCCGATTTCGCGGTTCCCGCCCTGCACGCCCTGCACGAAGCGGGGCACGAGATCGC
CGTGGTCTACAGCCAGCCGCCCCGTCCCGCCGGGCGCGGCCAGGCGGTGCGTCCCCAGCCCGTCCACCTGGCGGCCGAGG
CGCTGGGCATTCCGGTGCGCGTGCCCACCCGGCTGCGCGCCAATCACGACGAACATGCGTTCTTCCGCGCGCTGGACCTG
GATGCGGCGGTGGTCGCGGCCTACGGGCTGATCCTGCCCGGCGCGATGCTGACGCCCCGCGCCGGGGCTCGCCTGAACGT
CCATGCCAGCCTGCTGCCCCGCTGGCGGGGGGCCGCGCCGATCCAGGCCGCGATCCTGGCCGGCGACGACGAGAGCGGCG
TGACCATCATGCAGATGGACGAAGGGCTGGATACCGGCGCGATGCTGCTGACCGGCCGCGTCGCCCTGACGCCGGCAACC
ACCGCCAGCACGCTGCATGACGACCTGGCGGCCATGGGGGGCCGGCTGATCGTGGCGGCCCTGGCGAATTCGGAGACCGC
CGCGATTCCCCAGCCCGCCGAGGGCGCGACCTATGCCGCCCGCCTGACCCGCGAGGACGGACGCATCGACTGGACCCGCG
ATGCCGTGGATATCGACCGGCAGGTCCGCGCCCTGACGCCCTGGCCGGGCACCTTCACCACGCTGGACGGCACGGTCCTG
AAGATCGGCGCCGCGACCCCGATCGACGGGCCGCGGGACGCGGTACCCGGCACGGTGCTGGACGACCGGCTGACGGTCGC
CTGCGGCCAGGGGACCCTGCGCCTGACCCGCATCCAGCGCCCGGGACGCGGCATGATGGAGGCCGACGCCTTCCTGCGCG
GCCAGCCGGTCCCCGTCGGCACGCGGCTGGGATCGTGA

Upstream 100 bases:

>100_bases
GAAGCGCAACATGATCATGCGGCGCCTGGCCAAGGAACAGCGCCAGAAACGCTGAGTCTTTTCAGCGCTGATCATTCTCG
GGAAACAGGATCGGATCGTC

Downstream 100 bases:

>100_bases
CCGCGCCGTCGCTCCAGCGCTGGGCCGTACGCATCGAATATGACGGACGCCCCTTCGTCGGCTGGCAGCGCCAGATCACC
GGCCTGTCGGTCCAGCAGGT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRLAFMGTPDFAVPALHALHEAGHEIAVVYSQPPRPAGRGQAVRPQPVHLAAEALGIPVRVPTRLRANHDEHAFFRALDL
DAAVVAAYGLILPGAMLTPRAGARLNVHASLLPRWRGAAPIQAAILAGDDESGVTIMQMDEGLDTGAMLLTGRVALTPAT
TASTLHDDLAAMGGRLIVAALANSETAAIPQPAEGATYAARLTREDGRIDWTRDAVDIDRQVRALTPWPGTFTTLDGTVL
KIGAATPIDGPRDAVPGTVLDDRLTVACGQGTLRLTRIQRPGRGMMEADAFLRGQPVPVGTRLGS

Sequences:

>Translated_305_residues
MRLAFMGTPDFAVPALHALHEAGHEIAVVYSQPPRPAGRGQAVRPQPVHLAAEALGIPVRVPTRLRANHDEHAFFRALDL
DAAVVAAYGLILPGAMLTPRAGARLNVHASLLPRWRGAAPIQAAILAGDDESGVTIMQMDEGLDTGAMLLTGRVALTPAT
TASTLHDDLAAMGGRLIVAALANSETAAIPQPAEGATYAARLTREDGRIDWTRDAVDIDRQVRALTPWPGTFTTLDGTVL
KIGAATPIDGPRDAVPGTVLDDRLTVACGQGTLRLTRIQRPGRGMMEADAFLRGQPVPVGTRLGS
>Mature_305_residues
MRLAFMGTPDFAVPALHALHEAGHEIAVVYSQPPRPAGRGQAVRPQPVHLAAEALGIPVRVPTRLRANHDEHAFFRALDL
DAAVVAAYGLILPGAMLTPRAGARLNVHASLLPRWRGAAPIQAAILAGDDESGVTIMQMDEGLDTGAMLLTGRVALTPAT
TASTLHDDLAAMGGRLIVAALANSETAAIPQPAEGATYAARLTREDGRIDWTRDAVDIDRQVRALTPWPGTFTTLDGTVL
KIGAATPIDGPRDAVPGTVLDDRLTVACGQGTLRLTRIQRPGRGMMEADAFLRGQPVPVGTRLGS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=317, Percent_Identity=30.2839116719243, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI164663775, Length=237, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI238814322, Length=255, Percent_Identity=27.843137254902, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1789683, Length=302, Percent_Identity=43.7086092715232, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1788589, Length=315, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI133930964, Length=274, Percent_Identity=28.1021897810219, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319458, Length=269, Percent_Identity=29.368029739777, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45550868, Length=308, Percent_Identity=29.5454545454545, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=31.140350877193, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585660, Length=248, Percent_Identity=27.8225806451613, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_GLUDA (A9HKQ4)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602373.1
- RefSeq:   YP_002274758.1
- ProteinModelPortal:   A9HKQ4
- SMR:   A9HKQ4
- GeneID:   5792487
- GeneID:   6973741
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0347
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK936520
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32097; Mature: 32097

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAFMGTPDFAVPALHALHEAGHEIAVVYSQPPRPAGRGQAVRPQPVHLAAEALGIPVR
CEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHCCCEE
VPTRLRANHDEHAFFRALDLDAAVVAAYGLILPGAMLTPRAGARLNVHASLLPRWRGAAP
CCHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCCEEEEEHEECCCCCCCCC
IQAAILAGDDESGVTIMQMDEGLDTGAMLLTGRVALTPATTASTLHDDLAAMGGRLIVAA
EEEEEEECCCCCCEEEEEECCCCCCCEEEEECEEEECCCCCHHHHHHHHHHCCCEEEEEE
LANSETAAIPQPAEGATYAARLTREDGRIDWTRDAVDIDRQVRALTPWPGTFTTLDGTVL
ECCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCHHHHCCCEEEECCCCCCEEECCCEEE
KIGAATPIDGPRDAVPGTVLDDRLTVACGQGTLRLTRIQRPGRGMMEADAFLRGQPVPVG
EEECCCCCCCCCCCCCCCEECCCEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCCCCCC
TRLGS
CCCCC
>Mature Secondary Structure
MRLAFMGTPDFAVPALHALHEAGHEIAVVYSQPPRPAGRGQAVRPQPVHLAAEALGIPVR
CEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHCCCEE
VPTRLRANHDEHAFFRALDLDAAVVAAYGLILPGAMLTPRAGARLNVHASLLPRWRGAAP
CCHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCCEEEEEHEECCCCCCCCC
IQAAILAGDDESGVTIMQMDEGLDTGAMLLTGRVALTPATTASTLHDDLAAMGGRLIVAA
EEEEEEECCCCCCEEEEEECCCCCCCEEEEECEEEECCCCCHHHHHHHHHHCCCEEEEEE
LANSETAAIPQPAEGATYAARLTREDGRIDWTRDAVDIDRQVRALTPWPGTFTTLDGTVL
ECCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCHHHHCCCEEEECCCCCCEEECCCEEE
KIGAATPIDGPRDAVPGTVLDDRLTVACGQGTLRLTRIQRPGRGMMEADAFLRGQPVPVG
EEECCCCCCCCCCCCCCCEECCCEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCCCCCC
TRLGS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA