Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 162147911

GI number: 162147911

Start: 2182310

End: 2182849

Strand: Direct

Name: def [H]

Synonym: GDI_2127

Alternate gene names: 162147911

Gene position: 2182310-2182849 (Clockwise)

Preceding gene: 162147910

Following gene: 162147912

Centisome position: 55.33

GC content: 67.59

Gene sequence:

>540_bases
ATGATCGACCGTGACGCCATCGCCGCCGCCATTCCCATGCCCATCCTGGTCGCGCCGCAGGCCATCCTGCGGCAGAAGGC
GCGCCCCGTCCGGCCCGAGGATGCGGCGGGCGTGCGCGATGCCCTGCCGCGCATGTTCGCCGCCATGTACCAGGCCCCGG
GGATCGGCCTGGCGGCCCCGCAGGTCGGGATGGGCCTGCGATTTGCCATCGTGGATTTGGGCGAGGAAGGCGAGCGCCAG
CCGCTGATCCTGATCAACCCGGACGTGATCGCGGAATCGGACAGCCTGGCCTCGCGCGAGGAAGGGTGCCTGTCCCTGCC
CAACCAATATGCCGAGGTGATCCGCCCCGACCGTGTCCGCGTGCGCTACCGCACCCTGGACGGCACAGAGGAGGAACTGG
AGGCCGACGGCCTGCTGGCCACCTGCATCCAGCATGAGATCGATCATCTGGAAGGCATCCTGTTCGTCGATCACCTCTCG
ACGCTGAAGCGCAACATGATCATGCGGCGCCTGGCCAAGGAACAGCGCCAGAAACGCTGA

Upstream 100 bases:

>100_bases
ACGAAGAACTCATGATTTTCATCCAATTCTTCCGGGTTTTCCACGCTGCTGGGCGGGTTCCCTCTGGCGCGGCGGGGCCG
GTGTCGCTACATCGCCCTGC

Downstream 100 bases:

>100_bases
GTCTTTTCAGCGCTGATCATTCTCGGGAAACAGGATCGGATCGTCATGCGCCTGGCCTTCATGGGAACACCCGATTTCGC
GGTTCCCGCCCTGCACGCCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MIDRDAIAAAIPMPILVAPQAILRQKARPVRPEDAAGVRDALPRMFAAMYQAPGIGLAAPQVGMGLRFAIVDLGEEGERQ
PLILINPDVIAESDSLASREEGCLSLPNQYAEVIRPDRVRVRYRTLDGTEEELEADGLLATCIQHEIDHLEGILFVDHLS
TLKRNMIMRRLAKEQRQKR

Sequences:

>Translated_179_residues
MIDRDAIAAAIPMPILVAPQAILRQKARPVRPEDAAGVRDALPRMFAAMYQAPGIGLAAPQVGMGLRFAIVDLGEEGERQ
PLILINPDVIAESDSLASREEGCLSLPNQYAEVIRPDRVRVRYRTLDGTEEELEADGLLATCIQHEIDHLEGILFVDHLS
TLKRNMIMRRLAKEQRQKR
>Mature_179_residues
MIDRDAIAAAIPMPILVAPQAILRQKARPVRPEDAAGVRDALPRMFAAMYQAPGIGLAAPQVGMGLRFAIVDLGEEGERQ
PLILINPDVIAESDSLASREEGCLSLPNQYAEVIRPDRVRVRYRTLDGTEEELEADGLLATCIQHEIDHLEGILFVDHLS
TLKRNMIMRRLAKEQRQKR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=166, Percent_Identity=44.578313253012, Blast_Score=123, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=32.9268292682927, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645726, Length=155, Percent_Identity=30.9677419354839, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19927; Mature: 19927

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRDAIAAAIPMPILVAPQAILRQKARPVRPEDAAGVRDALPRMFAAMYQAPGIGLAAP
CCCHHHHHHHCCCCHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QVGMGLRFAIVDLGEEGERQPLILINPDVIAESDSLASREEGCLSLPNQYAEVIRPDRVR
CCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCEE
VRYRTLDGTEEELEADGLLATCIQHEIDHLEGILFVDHLSTLKRNMIMRRLAKEQRQKR
EEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDRDAIAAAIPMPILVAPQAILRQKARPVRPEDAAGVRDALPRMFAAMYQAPGIGLAAP
CCCHHHHHHHCCCCHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QVGMGLRFAIVDLGEEGERQPLILINPDVIAESDSLASREEGCLSLPNQYAEVIRPDRVR
CCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCEE
VRYRTLDGTEEELEADGLLATCIQHEIDHLEGILFVDHLSTLKRNMIMRRLAKEQRQKR
EEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]