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The map label for this gene is truA [H]

Identifier: 162147913

GI number: 162147913

Start: 2183809

End: 2184585

Strand: Direct

Name: truA [H]

Synonym: GDI_2129

Alternate gene names: 162147913

Gene position: 2183809-2184585 (Clockwise)

Preceding gene: 162147912

Following gene: 162147920

Centisome position: 55.37

GC content: 69.76

Gene sequence:

>777_bases
GTGACCGCGCCGTCGCTCCAGCGCTGGGCCGTACGCATCGAATATGACGGACGCCCCTTCGTCGGCTGGCAGCGCCAGAT
CACCGGCCTGTCGGTCCAGCAGGTACTGGAGGAAGCAGCGAGCCGGCTGGCGTCCGGACGCGTCGTGCCCAGCATTACCG
CCGGGCGCACCGATGCCGGCGTGCATGCCACCGGCCAGGCGGCGCACCTGGATTTTCCCGGCGACGTGCGCCTGAACGAT
TCGACCGTGCGCGATGCGCTGAATTTCCACATGAAGCCTTATCCGGTGGCGGTGCTGCGGGCCTGTCCGGTCGATACCGA
ATGGAGCGCGCGGTTTTCCGCCATCCGCCGGTCCTATCGCTATCGGATCCTGAACCGCCGCAGCCGGCCGACCCTGGATG
ACGGACGGGTCTGGCTGGTCAAGCGCACCCTGGACGAAGCGGCCATGGCGCGGGCCGCCCGCCTTCTGCTGGGACGGCAT
GATTTCACTTCGTTCCGCGCCAGCGCATGCCAGGCCAGGAGCCCGCTGCGGACGCTGGACCGGCTGGGCGTCGTCCGCCA
GGGCGAGGAAATCGTGATCGAGGCCGAGGCCCGGTCGTTCCTGCATCACCAGGTGCGCAACATGGTCGGCACATTGAAGC
TGGTGGGGGAAGGACTGTGGGCGCCCGACCGGGTGGCGGCGGCACTGGCCGCCTGCGACCGGCGGGCGGCGGGCCCGACA
GCCCCCCCGGACGGGTTGTACCTGACGGGGGTGGGCTACGACCCCGATCCGTTCTAA

Upstream 100 bases:

>100_bases
CCCTGCGCCTGACCCGCATCCAGCGCCCGGGACGCGGCATGATGGAGGCCGACGCCTTCCTGCGCGGCCAGCCGGTCCCC
GTCGGCACGCGGCTGGGATC

Downstream 100 bases:

>100_bases
GTCCCCTTACTGCGGAGCGTGCGCCGATGGCAGGGCCAGGAAATCCTGCCAGGGCGCATAATGGGGCGGCAGCACCGCCG
CCGCCTCGCCCAGGACCAGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTAPSLQRWAVRIEYDGRPFVGWQRQITGLSVQQVLEEAASRLASGRVVPSITAGRTDAGVHATGQAAHLDFPGDVRLND
STVRDALNFHMKPYPVAVLRACPVDTEWSARFSAIRRSYRYRILNRRSRPTLDDGRVWLVKRTLDEAAMARAARLLLGRH
DFTSFRASACQARSPLRTLDRLGVVRQGEEIVIEAEARSFLHHQVRNMVGTLKLVGEGLWAPDRVAAALAACDRRAAGPT
APPDGLYLTGVGYDPDPF

Sequences:

>Translated_258_residues
MTAPSLQRWAVRIEYDGRPFVGWQRQITGLSVQQVLEEAASRLASGRVVPSITAGRTDAGVHATGQAAHLDFPGDVRLND
STVRDALNFHMKPYPVAVLRACPVDTEWSARFSAIRRSYRYRILNRRSRPTLDDGRVWLVKRTLDEAAMARAARLLLGRH
DFTSFRASACQARSPLRTLDRLGVVRQGEEIVIEAEARSFLHHQVRNMVGTLKLVGEGLWAPDRVAAALAACDRRAAGPT
APPDGLYLTGVGYDPDPF
>Mature_257_residues
TAPSLQRWAVRIEYDGRPFVGWQRQITGLSVQQVLEEAASRLASGRVVPSITAGRTDAGVHATGQAAHLDFPGDVRLNDS
TVRDALNFHMKPYPVAVLRACPVDTEWSARFSAIRRSYRYRILNRRSRPTLDDGRVWLVKRTLDEAAMARAARLLLGRHD
FTSFRASACQARSPLRTLDRLGVVRQGEEIVIEAEARSFLHHQVRNMVGTLKLVGEGLWAPDRVAAALAACDRRAAGPTA
PPDGLYLTGVGYDPDPF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=281, Percent_Identity=36.6548042704626, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1788657, Length=255, Percent_Identity=42.7450980392157, Blast_Score=175, Evalue=2e-45,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=26.9736842105263, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28553; Mature: 28422

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSLQRWAVRIEYDGRPFVGWQRQITGLSVQQVLEEAASRLASGRVVPSITAGRTDAG
CCCCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCC
VHATGQAAHLDFPGDVRLNDSTVRDALNFHMKPYPVAVLRACPVDTEWSARFSAIRRSYR
CCCCCCEEECCCCCCEEECHHHHHHHHHCCCCCCCCHHHEECCCCCCHHHHHHHHHHHHH
YRILNRRSRPTLDDGRVWLVKRTLDEAAMARAARLLLGRHDFTSFRASACQARSPLRTLD
HHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
RLGVVRQGEEIVIEAEARSFLHHQVRNMVGTLKLVGEGLWAPDRVAAALAACDRRAAGPT
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
APPDGLYLTGVGYDPDPF
CCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TAPSLQRWAVRIEYDGRPFVGWQRQITGLSVQQVLEEAASRLASGRVVPSITAGRTDAG
CCCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCC
VHATGQAAHLDFPGDVRLNDSTVRDALNFHMKPYPVAVLRACPVDTEWSARFSAIRRSYR
CCCCCCEEECCCCCCEEECHHHHHHHHHCCCCCCCCHHHEECCCCCCHHHHHHHHHHHHH
YRILNRRSRPTLDDGRVWLVKRTLDEAAMARAARLLLGRHDFTSFRASACQARSPLRTLD
HHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
RLGVVRQGEEIVIEAEARSFLHHQVRNMVGTLKLVGEGLWAPDRVAAALAACDRRAAGPT
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
APPDGLYLTGVGYDPDPF
CCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA