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The map label for this gene is pdhC [H]

Identifier: 162147725

GI number: 162147725

Start: 1975914

End: 1977188

Strand: Direct

Name: pdhC [H]

Synonym: GDI_1941

Alternate gene names: 162147725

Gene position: 1975914-1977188 (Clockwise)

Preceding gene: 162147724

Following gene: 162147726

Centisome position: 50.1

GC content: 68.47

Gene sequence:

>1275_bases
ATGTCCGTGAATATCCTGATGCCGGCGCTGTCGCCGACGATGACCGAGGGCAAGCTGTCCCGCTGGCTGAAGAAGGAAGG
CGACGCGATCCATTCCGGCGACGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAGGTCGAGGCCGTGGATGACG
GCTTGCTGGGCCGCATCCTGGTTTCCGAAGGCACCGAAGGGGTGAAGGTCAACGCGCCGATCGCCATCGTGGTGGCGGAA
GGCGAGAGCGTTCCCGATGACGCAGCCCCCGTGGCGGCTGCTCCGGCGGCGGCTCCCGTGGCGGCGGCCCCGGTTTCCGA
GGCCAAGGCACCGGCGATCGCGGCCGCCCCGGCCGTGCCCCAGGGCGCGGCGCCGGCTCCGGCCCAGGGCACGCGCGTCT
TCGCGTCGCCGTTGGCGCGGCGCATCGCGGCGCAGAAGGGGATCGACCTGTCCGGCGTGAAGGGCAGCGGCCCGAATGGC
CGGATCGTGCGTCGCGACGTCGAATCCGCGACGGCGGCGCCCGTGGCGGCCCCGGTACCATCCCCGGCACCGTCCGCCCC
GGCCGCAGCGATCGAGGCGCCGCATACCGCCGTGCCGAACTCGACCATCCGCAAGGTCATCGCCCGGCGGCTGACCGAGG
CGAAGTCGACCATCCCGCATTTCTACGTGGCGATGGATGTGGAACTGGACGCGCTGCTGGACCTGCGGGCGAAGCTGAAC
GCGGCCTCGCCGGCCGAGGGACCGGGAGCGTTCAAGCTGTCGGTCAATGACATGCTGATCAAGGCGGTGGCGGTAACCCT
GCGTCGGGTGCCGAAGGTCAATGCATCCTATACCGAGGACGCGACGATCCTGTACGACGATGTCGATGTCTCGGTCGCCG
TGTCGATCGCCGATGGGCTGATCACGCCGATCGTGCGCCAGGCCGACCGCAAGTCGCTGCGCGAGATCAGCGAGGACGCG
AAGGATCTGATCACCCGCGCCCGCGCCGGCAAGCTGAAGCCGCAGGAATTTCAGGGCGGATCGTTCTCGATCTCGAACAT
GGGCATGTATGGGGTGAAGGAATTCTCGGCCATCATCAATCCGCCCCAGGCCGCCATCCTGGCCATCGCGGCGGCTGAGA
AGCGCGCCGTGGTCAAGGACGACGCGATCCGGATCGCCACCGTGATGACGGTGACGCTGTCGGTCGATCATCGCGTCGTC
GACGGCGCCCTGGCCGCCGAATGGGTTTCGACCTTCCGCTCGGTGGTCGAATCGCCGCTGAGCCTGGTGGTCTGA

Upstream 100 bases:

>100_bases
CGTGCCGATGCCCTTCGCCGCCAATCTTGAGAAGCTGGCCCTTCCCAACCCGACCTGGGTGGTGGATGCGGTCCGCAAGC
TGGTCTGAGGGAGCAGCGCG

Downstream 100 bases:

>100_bases
GGAACGCAGTTCATGAGCAAGACAGAGTTCGATATCGTCGTCATCGGCGGCGGTCCGGGTGGCTATGTCGCCGCCATCCG
GGCGGCCCAGCTTGGGCTCA

Product: dihydrolipoamid acetyltransferase component of pyruvate dehydrogenase complex

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSVNILMPALSPTMTEGKLSRWLKKEGDAIHSGDVIAEIETDKATMEVEAVDDGLLGRILVSEGTEGVKVNAPIAIVVAE
GESVPDDAAPVAAAPAAAPVAAAPVSEAKAPAIAAAPAVPQGAAPAPAQGTRVFASPLARRIAAQKGIDLSGVKGSGPNG
RIVRRDVESATAAPVAAPVPSPAPSAPAAAIEAPHTAVPNSTIRKVIARRLTEAKSTIPHFYVAMDVELDALLDLRAKLN
AASPAEGPGAFKLSVNDMLIKAVAVTLRRVPKVNASYTEDATILYDDVDVSVAVSIADGLITPIVRQADRKSLREISEDA
KDLITRARAGKLKPQEFQGGSFSISNMGMYGVKEFSAIINPPQAAILAIAAAEKRAVVKDDAIRIATVMTVTLSVDHRVV
DGALAAEWVSTFRSVVESPLSLVV

Sequences:

>Translated_424_residues
MSVNILMPALSPTMTEGKLSRWLKKEGDAIHSGDVIAEIETDKATMEVEAVDDGLLGRILVSEGTEGVKVNAPIAIVVAE
GESVPDDAAPVAAAPAAAPVAAAPVSEAKAPAIAAAPAVPQGAAPAPAQGTRVFASPLARRIAAQKGIDLSGVKGSGPNG
RIVRRDVESATAAPVAAPVPSPAPSAPAAAIEAPHTAVPNSTIRKVIARRLTEAKSTIPHFYVAMDVELDALLDLRAKLN
AASPAEGPGAFKLSVNDMLIKAVAVTLRRVPKVNASYTEDATILYDDVDVSVAVSIADGLITPIVRQADRKSLREISEDA
KDLITRARAGKLKPQEFQGGSFSISNMGMYGVKEFSAIINPPQAAILAIAAAEKRAVVKDDAIRIATVMTVTLSVDHRVV
DGALAAEWVSTFRSVVESPLSLVV
>Mature_423_residues
SVNILMPALSPTMTEGKLSRWLKKEGDAIHSGDVIAEIETDKATMEVEAVDDGLLGRILVSEGTEGVKVNAPIAIVVAEG
ESVPDDAAPVAAAPAAAPVAAAPVSEAKAPAIAAAPAVPQGAAPAPAQGTRVFASPLARRIAAQKGIDLSGVKGSGPNGR
IVRRDVESATAAPVAAPVPSPAPSAPAAAIEAPHTAVPNSTIRKVIARRLTEAKSTIPHFYVAMDVELDALLDLRAKLNA
ASPAEGPGAFKLSVNDMLIKAVAVTLRRVPKVNASYTEDATILYDDVDVSVAVSIADGLITPIVRQADRKSLREISEDAK
DLITRARAGKLKPQEFQGGSFSISNMGMYGVKEFSAIINPPQAAILAIAAAEKRAVVKDDAIRIATVMTVTLSVDHRVVD
GALAAEWVSTFRSVVESPLSLVV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=42.6966292134831, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI203098816, Length=464, Percent_Identity=38.3620689655172, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI203098753, Length=464, Percent_Identity=38.3620689655172, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=26.6203703703704, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=42.1383647798742, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI19923748, Length=258, Percent_Identity=29.8449612403101, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=32.9411764705882, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1786305, Length=423, Percent_Identity=29.7872340425532, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=44.2176870748299, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17538894, Length=331, Percent_Identity=36.5558912386707, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=418, Percent_Identity=30.1435406698565, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=27.4193548387097, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=42.5438596491228, Blast_Score=310, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6320352, Length=429, Percent_Identity=28.6713286713287, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=177, Percent_Identity=35.0282485875706, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20129315, Length=435, Percent_Identity=45.0574712643678, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24582497, Length=428, Percent_Identity=44.392523364486, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI18859875, Length=420, Percent_Identity=28.8095238095238, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=30.3964757709251, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43839; Mature: 43708

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNILMPALSPTMTEGKLSRWLKKEGDAIHSGDVIAEIETDKATMEVEAVDDGLLGRIL
CCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECCCEEEEEEECCCCCEEEEE
VSEGTEGVKVNAPIAIVVAEGESVPDDAAPVAAAPAAAPVAAAPVSEAKAPAIAAAPAVP
ECCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEECCCCC
QGAAPAPAQGTRVFASPLARRIAAQKGIDLSGVKGSGPNGRIVRRDVESATAAPVAAPVP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCC
SPAPSAPAAAIEAPHTAVPNSTIRKVIARRLTEAKSTIPHFYVAMDVELDALLDLRAKLN
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCC
AASPAEGPGAFKLSVNDMLIKAVAVTLRRVPKVNASYTEDATILYDDVDVSVAVSIADGL
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEHHHHH
ITPIVRQADRKSLREISEDAKDLITRARAGKLKPQEFQGGSFSISNMGMYGVKEFSAIIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEECCCCCHHHHHHHHHCC
PPQAAILAIAAAEKRAVVKDDAIRIATVMTVTLSVDHRVVDGALAAEWVSTFRSVVESPL
CCHHHEEEEEHHCCCCEECCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
SLVV
HHCC
>Mature Secondary Structure 
SVNILMPALSPTMTEGKLSRWLKKEGDAIHSGDVIAEIETDKATMEVEAVDDGLLGRIL
CEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECCCEEEEEEECCCCCEEEEE
VSEGTEGVKVNAPIAIVVAEGESVPDDAAPVAAAPAAAPVAAAPVSEAKAPAIAAAPAVP
ECCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEECCCCC
QGAAPAPAQGTRVFASPLARRIAAQKGIDLSGVKGSGPNGRIVRRDVESATAAPVAAPVP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCC
SPAPSAPAAAIEAPHTAVPNSTIRKVIARRLTEAKSTIPHFYVAMDVELDALLDLRAKLN
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCC
AASPAEGPGAFKLSVNDMLIKAVAVTLRRVPKVNASYTEDATILYDDVDVSVAVSIADGL
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEHHHHH
ITPIVRQADRKSLREISEDAKDLITRARAGKLKPQEFQGGSFSISNMGMYGVKEFSAIIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEECCCCCHHHHHHHHHCC
PPQAAILAIAAAEKRAVVKDDAIRIATVMTVTLSVDHRVVDGALAAEWVSTFRSVVESPL
CCHHHEEEEEHHCCCCEECCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
SLVV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]