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The map label for this gene is lpdA [H]

Identifier: 162147726

GI number: 162147726

Start: 1977201

End: 1978610

Strand: Direct

Name: lpdA [H]

Synonym: GDI_1942

Alternate gene names: 162147726

Gene position: 1977201-1978610 (Clockwise)

Preceding gene: 162147725

Following gene: 162147727

Centisome position: 50.13

GC content: 66.17

Gene sequence:

>1410_bases
ATGAGCAAGACAGAGTTCGATATCGTCGTCATCGGCGGCGGTCCGGGTGGCTATGTCGCCGCCATCCGGGCGGCCCAGCT
TGGGCTCAGCACCGCCGTGGTCGAAGCCAACCACCTGGGCGGCATCTGCCTCAACTGGGGCTGCATTCCCACCAAGGCGC
TGCTGCGGTCGTCGGAAATCAACCACCTGCTGCATAATCTGGGCGAATTCGGCTTCGCCGCCGACAATGTCCGCTTCGAC
CTGGACAAGGTGGTCAAACGCTCGCGCAAGGTCGCGGGCCAGCTTTCGGCCGGCGTCGCGCATCTGCTGAAGAAGAACAA
GGTCCCGGTGTTCGACGGTTTCGGCAAGCTGGCGGGCACGTCCGGCGGCCGCCGCAAGATCGAGGTCACCAAGGACGGCA
AGCCGGTCGCGACGCTGAGCGCCGCGAACGTCATCCTGGCGACCGGGGCCCGGGCCCGGGTCCTGCCGGGGCTGGAGCCG
GACGGCAAGCTGATCTGGTCCTATCGCGAGGCGATGGTACCCACCGAAATGCCGAAACGGCTGCTGGTGGTGGGGTCGGG
CGCGATCGGCATCGAATTCGCGTCCTTCTACCGCAACATGGGCGCCGAGGTGACGGTCGTCGAAGTCCTGGACCGCATCC
TGCCGGTCGAGGACGAAGAGATCAGCGCGCTGGCGCACAAGGCCTTCGTCAAGCAGGGTTTCACCTTGCTGACGGGGGCG
AAGATCGGCCCGATCCGCAAGAACGGTGACAGCGTCAGCCTGTCGGTCGAAGCGGGCGGCAAGACCCACGACATCACGGT
CGATCGCGTGATTTCAGCGGTCGGCATCGTGGGCAATGTGGAGAATATCGGCCTGGAGGGCACGGCGATCCAGGTCGACC
GCACCCACATCAAGGTCGATGCCTATTGCCGGACCGGCGAGCCCGGGGTGTATGCCATCGGCGACATCGCGGGGCCGCCA
TGGCTGGCGCACAAGGCCAGCCATGAAGGCGTGATGTGCGTCGAGGCGATCGCCGGGCGCCACGTCCACCCGATCGACCC
GCGGAACATTCCCGGGTGCACCTACTGCCGTCCACAGGTCGCCTCGGTCGGGATGACCGAGGCCGCGGCGAAGGCGGCGG
GCCATACGGTGCGCGTCGGGCGGTTTCCCTTCATCGGCAATGGCAAGGCCATCGCGATGGGAGAGCCCGAGGGCCTGGTC
AAGACGGTGTTCGACGCCAAGACGGGCGAATTGCTGGGCGCGCACATGATCGGGGCCGAGGTCACGGAGATGATCCAGGG
CTACGTCATCGCCCGGACGTCGGAACTGACCGACGCCGAGCTGAAGGAAACGGTCTTTCCGCACCCCACCATATCCGAAG
CGATGCACGAGGCGGTTCTGGCGGCCTATGACGGGGCGCTGCATATCTGA

Upstream 100 bases:

>100_bases
TCATCGCGTCGTCGACGGCGCCCTGGCCGCCGAATGGGTTTCGACCTTCCGCTCGGTGGTCGAATCGCCGCTGAGCCTGG
TGGTCTGAGGAACGCAGTTC

Downstream 100 bases:

>100_bases
AAGCCTGCCCGCAAATGCGCGCTGGCGGCGATCCTACGCGCGTTTCCTGTGCTCCGGTGCTCACGGCCTTCAAGGCCGCT
CCGCTCCGGTGCGCGGAAAC

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSKTEFDIVVIGGGPGGYVAAIRAAQLGLSTAVVEANHLGGICLNWGCIPTKALLRSSEINHLLHNLGEFGFAADNVRFD
LDKVVKRSRKVAGQLSAGVAHLLKKNKVPVFDGFGKLAGTSGGRRKIEVTKDGKPVATLSAANVILATGARARVLPGLEP
DGKLIWSYREAMVPTEMPKRLLVVGSGAIGIEFASFYRNMGAEVTVVEVLDRILPVEDEEISALAHKAFVKQGFTLLTGA
KIGPIRKNGDSVSLSVEAGGKTHDITVDRVISAVGIVGNVENIGLEGTAIQVDRTHIKVDAYCRTGEPGVYAIGDIAGPP
WLAHKASHEGVMCVEAIAGRHVHPIDPRNIPGCTYCRPQVASVGMTEAAAKAAGHTVRVGRFPFIGNGKAIAMGEPEGLV
KTVFDAKTGELLGAHMIGAEVTEMIQGYVIARTSELTDAELKETVFPHPTISEAMHEAVLAAYDGALHI

Sequences:

>Translated_469_residues
MSKTEFDIVVIGGGPGGYVAAIRAAQLGLSTAVVEANHLGGICLNWGCIPTKALLRSSEINHLLHNLGEFGFAADNVRFD
LDKVVKRSRKVAGQLSAGVAHLLKKNKVPVFDGFGKLAGTSGGRRKIEVTKDGKPVATLSAANVILATGARARVLPGLEP
DGKLIWSYREAMVPTEMPKRLLVVGSGAIGIEFASFYRNMGAEVTVVEVLDRILPVEDEEISALAHKAFVKQGFTLLTGA
KIGPIRKNGDSVSLSVEAGGKTHDITVDRVISAVGIVGNVENIGLEGTAIQVDRTHIKVDAYCRTGEPGVYAIGDIAGPP
WLAHKASHEGVMCVEAIAGRHVHPIDPRNIPGCTYCRPQVASVGMTEAAAKAAGHTVRVGRFPFIGNGKAIAMGEPEGLV
KTVFDAKTGELLGAHMIGAEVTEMIQGYVIARTSELTDAELKETVFPHPTISEAMHEAVLAAYDGALHI
>Mature_468_residues
SKTEFDIVVIGGGPGGYVAAIRAAQLGLSTAVVEANHLGGICLNWGCIPTKALLRSSEINHLLHNLGEFGFAADNVRFDL
DKVVKRSRKVAGQLSAGVAHLLKKNKVPVFDGFGKLAGTSGGRRKIEVTKDGKPVATLSAANVILATGARARVLPGLEPD
GKLIWSYREAMVPTEMPKRLLVVGSGAIGIEFASFYRNMGAEVTVVEVLDRILPVEDEEISALAHKAFVKQGFTLLTGAK
IGPIRKNGDSVSLSVEAGGKTHDITVDRVISAVGIVGNVENIGLEGTAIQVDRTHIKVDAYCRTGEPGVYAIGDIAGPPW
LAHKASHEGVMCVEAIAGRHVHPIDPRNIPGCTYCRPQVASVGMTEAAAKAAGHTVRVGRFPFIGNGKAIAMGEPEGLVK
TVFDAKTGELLGAHMIGAEVTEMIQGYVIARTSELTDAELKETVFPHPTISEAMHEAVLAAYDGALHI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=38.5438972162741, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=28.5714285714286, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI22035672, Length=470, Percent_Identity=27.4468085106383, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI291045266, Length=461, Percent_Identity=24.5119305856833, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=23.9316239316239, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=23.9316239316239, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=23.9316239316239, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=23.9316239316239, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=23.9316239316239, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI291045268, Length=457, Percent_Identity=24.507658643326, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=36.0869565217391, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=27.1948608137045, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=28.2167042889391, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87081717, Length=463, Percent_Identity=25.2699784017279, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=39.1025641025641, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=24.2171189979123, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=470, Percent_Identity=27.2340425531915, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=469, Percent_Identity=27.0788912579957, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=494, Percent_Identity=24.0890688259109, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=37.6050420168067, Blast_Score=269, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=26.5263157894737, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=26.8817204301075, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=39.9150743099788, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=26.7368421052632, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=26.7368421052632, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=26.7368421052632, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI17737741, Length=481, Percent_Identity=26.8191268191268, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49458; Mature: 49327

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTEFDIVVIGGGPGGYVAAIRAAQLGLSTAVVEANHLGGICLNWGCIPTKALLRSSEI
CCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHH
NHLLHNLGEFGFAADNVRFDLDKVVKRSRKVAGQLSAGVAHLLKKNKVPVFDGFGKLAGT
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHCCC
SGGRRKIEVTKDGKPVATLSAANVILATGARARVLPGLEPDGKLIWSYREAMVPTEMPKR
CCCCEEEEEECCCCCEEEEEECCEEEECCCCCEECCCCCCCCCEEEEHHHHCCCCCCCCE
LLVVGSGAIGIEFASFYRNMGAEVTVVEVLDRILPVEDEEISALAHKAFVKQGFTLLTGA
EEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECC
KIGPIRKNGDSVSLSVEAGGKTHDITVDRVISAVGIVGNVENIGLEGTAIQVDRTHIKVD
EECCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEEEEE
AYCRTGEPGVYAIGDIAGPPWLAHKASHEGVMCVEAIAGRHVHPIDPRNIPGCTYCRPQV
EEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCHH
ASVGMTEAAAKAAGHTVRVGRFPFIGNGKAIAMGEPEGLVKTVFDAKTGELLGAHMIGAE
HHCCCHHHHHHHCCCEEEECCCEEECCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHH
VTEMIQGYVIARTSELTDAELKETVFPHPTISEAMHEAVLAAYDGALHI
HHHHHCCEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKTEFDIVVIGGGPGGYVAAIRAAQLGLSTAVVEANHLGGICLNWGCIPTKALLRSSEI
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHH
NHLLHNLGEFGFAADNVRFDLDKVVKRSRKVAGQLSAGVAHLLKKNKVPVFDGFGKLAGT
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHCCC
SGGRRKIEVTKDGKPVATLSAANVILATGARARVLPGLEPDGKLIWSYREAMVPTEMPKR
CCCCEEEEEECCCCCEEEEEECCEEEECCCCCEECCCCCCCCCEEEEHHHHCCCCCCCCE
LLVVGSGAIGIEFASFYRNMGAEVTVVEVLDRILPVEDEEISALAHKAFVKQGFTLLTGA
EEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECC
KIGPIRKNGDSVSLSVEAGGKTHDITVDRVISAVGIVGNVENIGLEGTAIQVDRTHIKVD
EECCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEEEEE
AYCRTGEPGVYAIGDIAGPPWLAHKASHEGVMCVEAIAGRHVHPIDPRNIPGCTYCRPQV
EEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCHH
ASVGMTEAAAKAAGHTVRVGRFPFIGNGKAIAMGEPEGLVKTVFDAKTGELLGAHMIGAE
HHCCCHHHHHHHCCCEEEECCCEEECCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHH
VTEMIQGYVIARTSELTDAELKETVFPHPTISEAMHEAVLAAYDGALHI
HHHHHCCEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]