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The map label for this gene is pdhB [H]

Identifier: 162147724

GI number: 162147724

Start: 1974555

End: 1975901

Strand: Direct

Name: pdhB [H]

Synonym: GDI_1940

Alternate gene names: 162147724

Gene position: 1974555-1975901 (Clockwise)

Preceding gene: 162147723

Following gene: 162147725

Centisome position: 50.06

GC content: 68.3

Gene sequence:

>1347_bases
ATGACGCAGATCCTGATGCCGGCGCTTTCGCCCACCATGACCGAGGGCAAGCTGGCCCGCTGGCTGAAGACGACCGGCGA
CCATGTCGCCGCCGGGGACGTGATCGCGGAAATCGAGACCGACAAGGCGACGATGGAAGTCGAGGCCGTGGACGAGGGGA
CGCTGGGCGATATCCTGATCCCCGAAGGCACCGAGAACGTCCCGGTCAACACGCCGATCGCAACCCTGCAGTCCGAGGGC
GGTGCCGCGGCCCCCGCGGCGGCACCGGCCGCGAAGGCCCCGGCACCCCAGGCCGCTGCGGCGCCGGCCGCGCCGCAGCC
GGTCTCGCCCGTGGCGGCTCCCGTCGCGGCGCCTGAGAAGGATTGGGGCGAGACCGCCGAAATCACCGTGCGCGAAGCCC
TGCGCGACGCCATGGCCGCCGAACTGCGCCGCGACCAGGACGTGTTCCTGATCGGCGAGGAAGTCGCGCAGTACCAGGGC
GCCTACAAGGTGTCGCAGGGCCTGCTGGACGAATTCGGCGAAAAGCGCGTGATCGACACCCCGATCACCGAACAGGGCTT
CACCGGCATGGCGGTCGGCGCGGCGCTGACGGGCCTGAAGCCGATCGTCGAATTCATGACCATGAATTTCGCGATGCAGG
CGATCGACCAGATCATCAATTCGGCCGCCAAGACCCGCTACATGTCCGGCGGCCAGATGTCCTGCCCGATCGTCTTCCGT
GGCCCCAACGGGGCGGCGGCCCGCGTGGGTGCCCAGCATTCGCAGTGCTATGCCAGCTGGTACGGCCACGTGCCGGGGCT
GAAGGTGGTGGCGCCGTGGTCGGCGGCCGACGCCAAGGGCCTGCTGCGCGCCGCCATCCGCGACCCGAACCCGGTCATCG
TGCTGGAAAACGAGATCCTGTACGGCCAGAAGTTCCCGTGCCCGGTAGACGAGGATTTCATCCTGCCGATCGGCCGCGCC
AAGGTCGAGCGCGAGGGCAGGGACGTGACCATCGTCACCTTCTCGATCATGGTCGGCACGGCGCTGGAAGCCGCGGCGAT
CCTGGCCGAGCAGGGGATCGAGGCCGAGGTCATCAACCTGCGCACCATCCGCCCGCTGGATATCGAAACCATCGTGGCCA
GCGTGAAGAAGACCAGCCGCCTGGTCTGCGTCGAGGAAGGCTGGCCGTTCGCGGGGATCGGCGCCGAAGTGTCGATGCAG
GTCATCGAACATGCGTTCGACTATCTGGACGCGCCGCCTGCGCGGGTCGCCGGTGCCGACGTGCCGATGCCCTTCGCCGC
CAATCTTGAGAAGCTGGCCCTTCCCAACCCGACCTGGGTGGTGGATGCGGTCCGCAAGCTGGTCTGA

Upstream 100 bases:

>100_bases
GGTGAAGGAAGTGGTCGTGGACGCCGCCGATTTCGCGCAGACCAGCCCGGAGCCCGATCCGGCGGAACTGTGGACCGACG
TGCTGGTGGAGGGCTGAGCG

Downstream 100 bases:

>100_bases
GGGAGCAGCGCGATGTCCGTGAATATCCTGATGCCGGCGCTGTCGCCGACGATGACCGAGGGCAAGCTGTCCCGCTGGCT
GAAGAAGGAAGGCGACGCGA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTQILMPALSPTMTEGKLARWLKTTGDHVAAGDVIAEIETDKATMEVEAVDEGTLGDILIPEGTENVPVNTPIATLQSEG
GAAAPAAAPAAKAPAPQAAAAPAAPQPVSPVAAPVAAPEKDWGETAEITVREALRDAMAAELRRDQDVFLIGEEVAQYQG
AYKVSQGLLDEFGEKRVIDTPITEQGFTGMAVGAALTGLKPIVEFMTMNFAMQAIDQIINSAAKTRYMSGGQMSCPIVFR
GPNGAAARVGAQHSQCYASWYGHVPGLKVVAPWSAADAKGLLRAAIRDPNPVIVLENEILYGQKFPCPVDEDFILPIGRA
KVEREGRDVTIVTFSIMVGTALEAAAILAEQGIEAEVINLRTIRPLDIETIVASVKKTSRLVCVEEGWPFAGIGAEVSMQ
VIEHAFDYLDAPPARVAGADVPMPFAANLEKLALPNPTWVVDAVRKLV

Sequences:

>Translated_448_residues
MTQILMPALSPTMTEGKLARWLKTTGDHVAAGDVIAEIETDKATMEVEAVDEGTLGDILIPEGTENVPVNTPIATLQSEG
GAAAPAAAPAAKAPAPQAAAAPAAPQPVSPVAAPVAAPEKDWGETAEITVREALRDAMAAELRRDQDVFLIGEEVAQYQG
AYKVSQGLLDEFGEKRVIDTPITEQGFTGMAVGAALTGLKPIVEFMTMNFAMQAIDQIINSAAKTRYMSGGQMSCPIVFR
GPNGAAARVGAQHSQCYASWYGHVPGLKVVAPWSAADAKGLLRAAIRDPNPVIVLENEILYGQKFPCPVDEDFILPIGRA
KVEREGRDVTIVTFSIMVGTALEAAAILAEQGIEAEVINLRTIRPLDIETIVASVKKTSRLVCVEEGWPFAGIGAEVSMQ
VIEHAFDYLDAPPARVAGADVPMPFAANLEKLALPNPTWVVDAVRKLV
>Mature_447_residues
TQILMPALSPTMTEGKLARWLKTTGDHVAAGDVIAEIETDKATMEVEAVDEGTLGDILIPEGTENVPVNTPIATLQSEGG
AAAPAAAPAAKAPAPQAAAAPAAPQPVSPVAAPVAAPEKDWGETAEITVREALRDAMAAELRRDQDVFLIGEEVAQYQGA
YKVSQGLLDEFGEKRVIDTPITEQGFTGMAVGAALTGLKPIVEFMTMNFAMQAIDQIINSAAKTRYMSGGQMSCPIVFRG
PNGAAARVGAQHSQCYASWYGHVPGLKVVAPWSAADAKGLLRAAIRDPNPVIVLENEILYGQKFPCPVDEDFILPIGRAK
VEREGRDVTIVTFSIMVGTALEAAAILAEQGIEAEVINLRTIRPLDIETIVASVKKTSRLVCVEEGWPFAGIGAEVSMQV
IEHAFDYLDAPPARVAGADVPMPFAANLEKLALPNPTWVVDAVRKLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=61.1111111111111, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=57.4074074074074, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI4557353, Length=334, Percent_Identity=37.125748502994, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI34101272, Length=334, Percent_Identity=37.125748502994, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI31711992, Length=74, Percent_Identity=54.0540540540541, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI203098753, Length=94, Percent_Identity=42.5531914893617, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI203098816, Length=78, Percent_Identity=47.4358974358974, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI260898739, Length=57, Percent_Identity=54.3859649122807, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=63.7770897832817, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17506935, Length=327, Percent_Identity=37.9204892966361, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17560088, Length=119, Percent_Identity=40.3361344537815, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319698, Length=347, Percent_Identity=61.3832853025937, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6324258, Length=137, Percent_Identity=42.3357664233577, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321632, Length=71, Percent_Identity=49.2957746478873, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=61.3496932515337, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=61.3496932515337, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI160714832, Length=328, Percent_Identity=36.890243902439, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI160714828, Length=328, Percent_Identity=36.890243902439, Blast_Score=208, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=65.9340659340659, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=65.9340659340659, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI20129315, Length=82, Percent_Identity=46.3414634146341, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 47420; Mature: 47289

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQILMPALSPTMTEGKLARWLKTTGDHVAAGDVIAEIETDKATMEVEAVDEGTLGDILI
CCCEECCCCCCCCCHHHHHHHHHCCCCCEECCCEEEEEECCCEEEEEEECCCCCCCCEEC
PEGTENVPVNTPIATLQSEGGAAAPAAAPAAKAPAPQAAAAPAAPQPVSPVAAPVAAPEK
CCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DWGETAEITVREALRDAMAAELRRDQDVFLIGEEVAQYQGAYKVSQGLLDEFGEKRVIDT
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHHHHHHHHHCCCEEECC
PITEQGFTGMAVGAALTGLKPIVEFMTMNFAMQAIDQIINSAAKTRYMSGGQMSCPIVFR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEE
GPNGAAARVGAQHSQCYASWYGHVPGLKVVAPWSAADAKGLLRAAIRDPNPVIVLENEIL
CCCCCHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCEE
YGQKFPCPVDEDFILPIGRAKVEREGRDVTIVTFSIMVGTALEAAAILAEQGIEAEVINL
ECCCCCCCCCCCCEECCCCHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEE
RTIRPLDIETIVASVKKTSRLVCVEEGWPFAGIGAEVSMQVIEHAFDYLDAPPARVAGAD
EECCCCCHHHHHHHHHHCCEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCHHCCCC
VPMPFAANLEKLALPNPTWVVDAVRKLV
CCCCHHCCCHHHCCCCCCHHHHHHHHHC
>Mature Secondary Structure 
TQILMPALSPTMTEGKLARWLKTTGDHVAAGDVIAEIETDKATMEVEAVDEGTLGDILI
CCEECCCCCCCCCHHHHHHHHHCCCCCEECCCEEEEEECCCEEEEEEECCCCCCCCEEC
PEGTENVPVNTPIATLQSEGGAAAPAAAPAAKAPAPQAAAAPAAPQPVSPVAAPVAAPEK
CCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DWGETAEITVREALRDAMAAELRRDQDVFLIGEEVAQYQGAYKVSQGLLDEFGEKRVIDT
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHHHHHHHHHCCCEEECC
PITEQGFTGMAVGAALTGLKPIVEFMTMNFAMQAIDQIINSAAKTRYMSGGQMSCPIVFR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEE
GPNGAAARVGAQHSQCYASWYGHVPGLKVVAPWSAADAKGLLRAAIRDPNPVIVLENEIL
CCCCCHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCEE
YGQKFPCPVDEDFILPIGRAKVEREGRDVTIVTFSIMVGTALEAAAILAEQGIEAEVINL
ECCCCCCCCCCCCEECCCCHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEE
RTIRPLDIETIVASVKKTSRLVCVEEGWPFAGIGAEVSMQVIEHAFDYLDAPPARVAGAD
EECCCCCHHHHHHHHHHCCEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCHHCCCC
VPMPFAANLEKLALPNPTWVVDAVRKLV
CCCCHHCCCHHHCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]