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The map label for this gene is argG [H]

Identifier: 162147641

GI number: 162147641

Start: 1894492

End: 1895721

Strand: Reverse

Name: argG [H]

Synonym: GDI_1857

Alternate gene names: 162147641

Gene position: 1895721-1894492 (Counterclockwise)

Preceding gene: 162147642

Following gene: 162147640

Centisome position: 48.06

GC content: 64.63

Gene sequence:

>1230_bases
ATGGCCGTAAAGGACGTCAAGAAGGTCGTGCTCGCCTATTCCGGCGGGCTCGATACATCGGTAATTCTGCGCTGGCTGCA
GACCACCTACGGGTGCGAGGTCGTCACCTTCACCGCCGACCTCGGCCAGGGCGAGGAACTGGAACCCGCCCGCAAGAAGG
CCGAAATGTTCGGCGTGAAGGAAATCTTCGTCGAGGACCTGCGCGAGACCTTCGTCAAGGACTTCGTCTTCCCGATGTTC
CGCGCCAACACGCTGTATGAAGGCCAGTACCTGCTGGGGACCTCCATCGCGCGTCCGCTGATCGCCCAGCGCCAGATCGA
AATCGCCGAGGCCGTGGGTGCCGACGCCGTGGCCCATGGCGCGACGGGCAAGGGCAACGACCAGGTGCGCTTCGAACTGG
CCTATTACGCGCTGAAGCCCGACGTGACGGTCATCTCCCCCTGGCGGGAATGGGACCTGACCTCGCGCACGCGGCTGCTG
GCCTTCGCCGAGGAACATCAGATCCCTATCGCGAAGGACAAGCGCGGCGAGGCCCCGTTCTCGGTCGATGCCAACCTGCT
GCACTCCTCGTCCGAAGGCAAGCTGCTGGAAGACCCTGCCGTCGCACCCGATGAAATCGTCTTCCAGCGCACGATCTCGC
CCGAGGCCGCGCCGGACGTCGCGACCGAAATCGCGATCGATTTCGTCTCGGGCGACCCGGTGGCGCTGAACGGCGTCACC
CTGTCCCCCGCCACGCTGCTGGCGCGGCTGAACGAACTGGGCAAGGCCAACGGGATCGGGCGGCTGGACCTGGTGGAAAA
CCGCTTCGTCGGCATGAAGTCGCGCGGCATCTACGAAACGCCGGGCGGCACCATCCTGCTGGCGGCGCATCGCAGCATGG
AAACCATCACGCTGGACCGCGAGGCCGGGCACCTGAAGGACAGCCTGATGCCCCGCTATGCCGAACTGATCTATAACGGC
TTCTGGTTCTCGCCCGAGCGGCGCATGCTCCAGGCCCTGATCGACGAAAGCCAGCATTCCGTGACCGGACGCGTGCGGCT
GAAGCTGTACAAGGGCAATGTGATCTGCGTCGGGCGGGAAAGCCCCCATAGCCTGTACGATACCCGCGTTGTGACATTCG
AAGACGACGAAGGGGCGTATAATCAAAGCGATGCACTGGGCTTCATCAAGCTGAACGCCCTGCGTCTGCGTCTGGGCGCG
CAGATCGGACGGCGCGGCGGCGCGCTGTAG

Upstream 100 bases:

>100_bases
CGCGCCCGCTGGCACCGGCGGCGATCCGGTGCTAGGACCCGGAACATCGGTGCGGAACGCCGCATCATCGCGCAGCCTCG
ACGACAGGAGTGTCCTCCCC

Downstream 100 bases:

>100_bases
GATCGCGTCCCGCATGTCGTTCCATTCGTCCTGTCTGACCGAGGTGTCATGAAACGTCTGTCCCGCTTCATTCCCCTCCT
TCTCGCTTCTTCCCTGGCCC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MAVKDVKKVVLAYSGGLDTSVILRWLQTTYGCEVVTFTADLGQGEELEPARKKAEMFGVKEIFVEDLRETFVKDFVFPMF
RANTLYEGQYLLGTSIARPLIAQRQIEIAEAVGADAVAHGATGKGNDQVRFELAYYALKPDVTVISPWREWDLTSRTRLL
AFAEEHQIPIAKDKRGEAPFSVDANLLHSSSEGKLLEDPAVAPDEIVFQRTISPEAAPDVATEIAIDFVSGDPVALNGVT
LSPATLLARLNELGKANGIGRLDLVENRFVGMKSRGIYETPGGTILLAAHRSMETITLDREAGHLKDSLMPRYAELIYNG
FWFSPERRMLQALIDESQHSVTGRVRLKLYKGNVICVGRESPHSLYDTRVVTFEDDEGAYNQSDALGFIKLNALRLRLGA
QIGRRGGAL

Sequences:

>Translated_409_residues
MAVKDVKKVVLAYSGGLDTSVILRWLQTTYGCEVVTFTADLGQGEELEPARKKAEMFGVKEIFVEDLRETFVKDFVFPMF
RANTLYEGQYLLGTSIARPLIAQRQIEIAEAVGADAVAHGATGKGNDQVRFELAYYALKPDVTVISPWREWDLTSRTRLL
AFAEEHQIPIAKDKRGEAPFSVDANLLHSSSEGKLLEDPAVAPDEIVFQRTISPEAAPDVATEIAIDFVSGDPVALNGVT
LSPATLLARLNELGKANGIGRLDLVENRFVGMKSRGIYETPGGTILLAAHRSMETITLDREAGHLKDSLMPRYAELIYNG
FWFSPERRMLQALIDESQHSVTGRVRLKLYKGNVICVGRESPHSLYDTRVVTFEDDEGAYNQSDALGFIKLNALRLRLGA
QIGRRGGAL
>Mature_408_residues
AVKDVKKVVLAYSGGLDTSVILRWLQTTYGCEVVTFTADLGQGEELEPARKKAEMFGVKEIFVEDLRETFVKDFVFPMFR
ANTLYEGQYLLGTSIARPLIAQRQIEIAEAVGADAVAHGATGKGNDQVRFELAYYALKPDVTVISPWREWDLTSRTRLLA
FAEEHQIPIAKDKRGEAPFSVDANLLHSSSEGKLLEDPAVAPDEIVFQRTISPEAAPDVATEIAIDFVSGDPVALNGVTL
SPATLLARLNELGKANGIGRLDLVENRFVGMKSRGIYETPGGTILLAAHRSMETITLDREAGHLKDSLMPRYAELIYNGF
WFSPERRMLQALIDESQHSVTGRVRLKLYKGNVICVGRESPHSLYDTRVVTFEDDEGAYNQSDALGFIKLNALRLRLGAQ
IGRRGGAL

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=46.4824120603015, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=46.4824120603015, Blast_Score=353, Evalue=1e-97,
Organism=Escherichia coli, GI1789563, Length=400, Percent_Identity=26.75, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=406, Percent_Identity=45.320197044335, Blast_Score=335, Evalue=8e-93,
Organism=Drosophila melanogaster, GI21358151, Length=403, Percent_Identity=44.4168734491315, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45145; Mature: 45014

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKDVKKVVLAYSGGLDTSVILRWLQTTYGCEVVTFTADLGQGEELEPARKKAEMFGVK
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
EIFVEDLRETFVKDFVFPMFRANTLYEGQYLLGTSIARPLIAQRQIEIAEAVGADAVAHG
HHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCC
ATGKGNDQVRFELAYYALKPDVTVISPWREWDLTSRTRLLAFAEEHQIPIAKDKRGEAPF
CCCCCCCEEEEEEEEEEECCCEEEECCCHHCCCCCCCEEEEEHHHCCCCCCCCCCCCCCC
SVDANLLHSSSEGKLLEDPAVAPDEIVFQRTISPEAAPDVATEIAIDFVSGDPVALNGVT
CCCHHHEECCCCCCEECCCCCCCCHHEEEECCCCCCCCCHHHEEEEEEECCCCEEEECEE
LSPATLLARLNELGKANGIGRLDLVENRFVGMKSRGIYETPGGTILLAAHRSMETITLDR
ECHHHHHHHHHHHHCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEECCCCEEEEEEC
EAGHLKDSLMPRYAELIYNGFWFSPERRMLQALIDESQHSVTGRVRLKLYKGNVICVGRE
CCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCCEEEEEEEEEEECCEEEEECC
SPHSLYDTRVVTFEDDEGAYNQSDALGFIKLNALRLRLGAQIGRRGGAL
CCCCHHCEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AVKDVKKVVLAYSGGLDTSVILRWLQTTYGCEVVTFTADLGQGEELEPARKKAEMFGVK
CHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
EIFVEDLRETFVKDFVFPMFRANTLYEGQYLLGTSIARPLIAQRQIEIAEAVGADAVAHG
HHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCC
ATGKGNDQVRFELAYYALKPDVTVISPWREWDLTSRTRLLAFAEEHQIPIAKDKRGEAPF
CCCCCCCEEEEEEEEEEECCCEEEECCCHHCCCCCCCEEEEEHHHCCCCCCCCCCCCCCC
SVDANLLHSSSEGKLLEDPAVAPDEIVFQRTISPEAAPDVATEIAIDFVSGDPVALNGVT
CCCHHHEECCCCCCEECCCCCCCCHHEEEECCCCCCCCCHHHEEEEEEECCCCEEEECEE
LSPATLLARLNELGKANGIGRLDLVENRFVGMKSRGIYETPGGTILLAAHRSMETITLDR
ECHHHHHHHHHHHHCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEECCCCEEEEEEC
EAGHLKDSLMPRYAELIYNGFWFSPERRMLQALIDESQHSVTGRVRLKLYKGNVICVGRE
CCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCCEEEEEEEEEEECCEEEEECC
SPHSLYDTRVVTFEDDEGAYNQSDALGFIKLNALRLRLGAQIGRRGGAL
CCCCHHCEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA