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The map label for this gene is rlmN [H]

Identifier: 162147642

GI number: 162147642

Start: 1895855

End: 1897069

Strand: Reverse

Name: rlmN [H]

Synonym: GDI_1858

Alternate gene names: 162147642

Gene position: 1897069-1895855 (Counterclockwise)

Preceding gene: 162147643

Following gene: 162147641

Centisome position: 48.1

GC content: 66.09

Gene sequence:

>1215_bases
ATGACCGCCGTTCCCGCCCCCGCCCAGCAGCCCGCCGAAACCGGACTGGCGGCCTCGATGACCGAGGTCGAACGAAAGCG
GATTCTGGCAAAATCCGCGCTGTTCGCCCCACCGGCCGGCGTGCTGCCCGACGGACGGCGCGAACTGGTCGGCCTGTCGC
GCGAAGAACTGACGCAGGCGCTGGTGGAAATCGGCGAGAAGCCCTTTCGTACCAAGCAGTTGTGGCACTGGATCTATCAC
CAGGGCGTCACGGATTTCGCACGCATGTCCTCGATCGCCAAGCCGTTGCAGGCGAAGCTGGCCGAGCGTTTCGTCGTCGG
CCGGCCCGATGCGGCGATGGTGCAGACCTCGACGGACGAGACCCGCAAGTTCCTCTTCCGCTTCCGCGACGGGCAGGAGG
CCGAGACGGTCTATATCCCCGACCGGCGCGAGGATCGGGGCGCTGTCTGCATTTCCTCGCAGGTCGGCTGCACGCTGTCC
TGCACCTTCTGCCATACCGGCACGCAGAAGCTGGTGCGCAACCTGGGGGCGGCGGAAATCGTCGGCCAGTTCATGGCGGC
ACGCGACAGCTACGGCGAATGGCCCAGCCCAAAGGGCGACACGCCGCGCCTGCTGTCGACCATCGTGCTGATGGGCATGG
GCGAGCCGCTGTATAATTACGAGAACATCGCCAAGGCGATGAAGATCATCATGGATGGCGAGGGCATCGGCCTGTCGCGC
CGCCGCATTACCCTGTCCACCTCGGGCGTGATCCCGATGATGGACCAGTGCGGGTCCGAACTGGGAATCAACCTCGCGAT
CTCGCTGCACGCGGTGCGCAACGACCTGCGTGACGAGATCGTCCCGCTGAACCGCAAATATCCGATCGAGGACCTGATCG
CGGCGTGCCGCCGCTATCCCACCGCCAGCAACGCACGGCGCATCACGTTCGAATACATCATGTTGCGCGGCGTGAATGAC
AGCGAGGCCGAAGCCCGCGAACTGGTCCGCCTGATCGCCGGCATCCCCGCGAAGGTCAACCTGATCCCGTTCAACCCGTG
GCCCGGCAGCGCCTACAAGCCGTCCACGCGCGAACAACTGGCGAAATTCGCCCAGATCGTGATGGATGCGGGCTTCGCCT
CGCCGATCCGCATGCCGCGCGGGCGCGACATCCTGGCCGCGTGCGGCCAGTTGCGGACGGAAAGCGAGCGCGAGCGCCGG
AACGCCACGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCGGCGGGACGGATAGTAATTCCCCCATTTGCGTCCGGATGTGATAGGCAGCGCGGATTCCGCCCTTCGCCCACGTC
CGTTGCATGAGTACCTGTCC

Downstream 100 bases:

>100_bases
GCCCCGGACACAGGCACGCCGCCGAATTGGCGGCGCGCCCGCTGGCACCGGCGGCGATCCGGTGCTAGGACCCGGAACAT
CGGTGCGGAACGCCGCATCA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTAVPAPAQQPAETGLAASMTEVERKRILAKSALFAPPAGVLPDGRRELVGLSREELTQALVEIGEKPFRTKQLWHWIYH
QGVTDFARMSSIAKPLQAKLAERFVVGRPDAAMVQTSTDETRKFLFRFRDGQEAETVYIPDRREDRGAVCISSQVGCTLS
CTFCHTGTQKLVRNLGAAEIVGQFMAARDSYGEWPSPKGDTPRLLSTIVLMGMGEPLYNYENIAKAMKIIMDGEGIGLSR
RRITLSTSGVIPMMDQCGSELGINLAISLHAVRNDLRDEIVPLNRKYPIEDLIAACRRYPTASNARRITFEYIMLRGVND
SEAEARELVRLIAGIPAKVNLIPFNPWPGSAYKPSTREQLAKFAQIVMDAGFASPIRMPRGRDILAACGQLRTESERERR
NATA

Sequences:

>Translated_404_residues
MTAVPAPAQQPAETGLAASMTEVERKRILAKSALFAPPAGVLPDGRRELVGLSREELTQALVEIGEKPFRTKQLWHWIYH
QGVTDFARMSSIAKPLQAKLAERFVVGRPDAAMVQTSTDETRKFLFRFRDGQEAETVYIPDRREDRGAVCISSQVGCTLS
CTFCHTGTQKLVRNLGAAEIVGQFMAARDSYGEWPSPKGDTPRLLSTIVLMGMGEPLYNYENIAKAMKIIMDGEGIGLSR
RRITLSTSGVIPMMDQCGSELGINLAISLHAVRNDLRDEIVPLNRKYPIEDLIAACRRYPTASNARRITFEYIMLRGVND
SEAEARELVRLIAGIPAKVNLIPFNPWPGSAYKPSTREQLAKFAQIVMDAGFASPIRMPRGRDILAACGQLRTESERERR
NATA
>Mature_403_residues
TAVPAPAQQPAETGLAASMTEVERKRILAKSALFAPPAGVLPDGRRELVGLSREELTQALVEIGEKPFRTKQLWHWIYHQ
GVTDFARMSSIAKPLQAKLAERFVVGRPDAAMVQTSTDETRKFLFRFRDGQEAETVYIPDRREDRGAVCISSQVGCTLSC
TFCHTGTQKLVRNLGAAEIVGQFMAARDSYGEWPSPKGDTPRLLSTIVLMGMGEPLYNYENIAKAMKIIMDGEGIGLSRR
RITLSTSGVIPMMDQCGSELGINLAISLHAVRNDLRDEIVPLNRKYPIEDLIAACRRYPTASNARRITFEYIMLRGVNDS
EAEARELVRLIAGIPAKVNLIPFNPWPGSAYKPSTREQLAKFAQIVMDAGFASPIRMPRGRDILAACGQLRTESERERRN
ATA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=360, Percent_Identity=44.4444444444444, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 44766; Mature: 44635

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVPAPAQQPAETGLAASMTEVERKRILAKSALFAPPAGVLPDGRRELVGLSREELTQA
CCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHH
LVEIGEKPFRTKQLWHWIYHQGVTDFARMSSIAKPLQAKLAERFVVGRPDAAMVQTSTDE
HHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCHHH
TRKFLFRFRDGQEAETVYIPDRREDRGAVCISSQVGCTLSCTFCHTGTQKLVRNLGAAEI
HHHHHHHHCCCCCCCEEECCCCCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHCCHHHH
VGQFMAARDSYGEWPSPKGDTPRLLSTIVLMGMGEPLYNYENIAKAMKIIMDGEGIGLSR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
RRITLSTSGVIPMMDQCGSELGINLAISLHAVRNDLRDEIVPLNRKYPIEDLIAACRRYP
EEEEEECCCCCCCHHHCCHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
TASNARRITFEYIMLRGVNDSEAEARELVRLIAGIPAKVNLIPFNPWPGSAYKPSTREQL
CCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
AKFAQIVMDAGFASPIRMPRGRDILAACGQLRTESERERRNATA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure 
TAVPAPAQQPAETGLAASMTEVERKRILAKSALFAPPAGVLPDGRRELVGLSREELTQA
CCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHH
LVEIGEKPFRTKQLWHWIYHQGVTDFARMSSIAKPLQAKLAERFVVGRPDAAMVQTSTDE
HHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCHHH
TRKFLFRFRDGQEAETVYIPDRREDRGAVCISSQVGCTLSCTFCHTGTQKLVRNLGAAEI
HHHHHHHHCCCCCCCEEECCCCCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHCCHHHH
VGQFMAARDSYGEWPSPKGDTPRLLSTIVLMGMGEPLYNYENIAKAMKIIMDGEGIGLSR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
RRITLSTSGVIPMMDQCGSELGINLAISLHAVRNDLRDEIVPLNRKYPIEDLIAACRRYP
EEEEEECCCCCCCHHHCCHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
TASNARRITFEYIMLRGVNDSEAEARELVRLIAGIPAKVNLIPFNPWPGSAYKPSTREQL
CCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
AKFAQIVMDAGFASPIRMPRGRDILAACGQLRTESERERRNATA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA