Click here to switch to the map view.

The map label for this gene is hdhA [C]

Identifier: 162147027

GI number: 162147027

Start: 1224699

End: 1225661

Strand: Reverse

Name: hdhA [C]

Synonym: GDI_1232

Alternate gene names: 162147027

Gene position: 1225661-1224699 (Counterclockwise)

Preceding gene: 162147028

Following gene: 162147024

Centisome position: 31.08

GC content: 73.31

Gene sequence:

>963_bases
ATGAGCGTACCCCTGAAATGGACGATCGACCGTGCCCCCGACCTGACCGGCCGCGTCGCCGTGGTGACGGGGGCGACCGG
CGGGCTAGGGTACGAGACCGCATGCGGCCTGGCGTCGCGCGGGGCCACGGTCATCCTGACCGGGCGCAATGCCGATCGCG
GCGCCGCCGCCCTGGCGGGGCTGCGGACGCGAGTTGCGGATGCGCGCGCCGAATTCATGGTGCTGGACCTGGCCAGCCTG
CGGTCGATCGCCGATTTCGCGGGCGACCTGACCGAGCGGCTGAAGGGGCAGGGACCGGGGGCGGTGGACATCCTGGTGAA
CAATGCGGGCGTGATGGCCCCGCCCCGGCGACAGGAAACCGAAGACGGGTTCGAACTGCAGTTCGGCACCAATTACCTGG
GCCATTTCGCCCTGACCGGGCGGCTGCGGCCGCTGCTGGTCCGGGCCCCGGGCGGGGCGCGGGTCGTCACCGTCGCCAGC
CTGGCGGCCCGGCAGGGGCACATCACGTTCGACGATCTGCAGGCACGGCATCGCTACAGCCCCTTCGGGGCCTACCAGCA
GAGCAAGCTGGCCAACCTGATCTTCGCGCTGGAACTGGACCGGCTGGCACAGTCCGGGGGCTGGAAGCTGCATTCCATCG
CCGCCCATCCGGGCTGGTCGCAGACCGACCTGGCCATCAGCCGCTCGGCAGCGCGGCAGGGCGTGGCGGAACGCCTGCTG
CGCTGCCTGACGGAGGTCGCGTTCCGGACGCTGGGCCAGTCGGCCGCGGCCGGGGCGCGGCCGATCCTGTTTGCGGCAGC
GGGGGCCGAGGCCGTGGATGGCGGGTATTACGGCCCCGGGGGACGGGGCGAACGGCGGGGCCGGGTGACGGATGCGCGCG
TCCCGGCGCAGGCCCGCAACCTGCCCGTGGCGCGCCGCCTGTGGGCGGTGTCCGAGCGGCTGACCGGGGTGGCGCTGTCG
TAG

Upstream 100 bases:

>100_bases
TCATCGGCGGCAGTTTCGTCTTCCTGAAGAAGAACGAACGGCGCCTGACCGAGGAAGCGACCCGGGAGGCCGAACGGGAA
CAGCAGAAGGAGCCTGCGCG

Downstream 100 bases:

>100_bases
GGAAGCCGGAATCGGTTTCCAAAGGCCCCCGGCCTTTGGCGGGGCATGGGGCAGAGCCCCATATCTATCACGGCACCAGG
ATCGTACTCCCTACCGTCGT

Product: dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSVPLKWTIDRAPDLTGRVAVVTGATGGLGYETACGLASRGATVILTGRNADRGAAALAGLRTRVADARAEFMVLDLASL
RSIADFAGDLTERLKGQGPGAVDILVNNAGVMAPPRRQETEDGFELQFGTNYLGHFALTGRLRPLLVRAPGGARVVTVAS
LAARQGHITFDDLQARHRYSPFGAYQQSKLANLIFALELDRLAQSGGWKLHSIAAHPGWSQTDLAISRSAARQGVAERLL
RCLTEVAFRTLGQSAAAGARPILFAAAGAEAVDGGYYGPGGRGERRGRVTDARVPAQARNLPVARRLWAVSERLTGVALS

Sequences:

>Translated_320_residues
MSVPLKWTIDRAPDLTGRVAVVTGATGGLGYETACGLASRGATVILTGRNADRGAAALAGLRTRVADARAEFMVLDLASL
RSIADFAGDLTERLKGQGPGAVDILVNNAGVMAPPRRQETEDGFELQFGTNYLGHFALTGRLRPLLVRAPGGARVVTVAS
LAARQGHITFDDLQARHRYSPFGAYQQSKLANLIFALELDRLAQSGGWKLHSIAAHPGWSQTDLAISRSAARQGVAERLL
RCLTEVAFRTLGQSAAAGARPILFAAAGAEAVDGGYYGPGGRGERRGRVTDARVPAQARNLPVARRLWAVSERLTGVALS
>Mature_319_residues
SVPLKWTIDRAPDLTGRVAVVTGATGGLGYETACGLASRGATVILTGRNADRGAAALAGLRTRVADARAEFMVLDLASLR
SIADFAGDLTERLKGQGPGAVDILVNNAGVMAPPRRQETEDGFELQFGTNYLGHFALTGRLRPLLVRAPGGARVVTVASL
AARQGHITFDDLQARHRYSPFGAYQQSKLANLIFALELDRLAQSGGWKLHSIAAHPGWSQTDLAISRSAARQGVAERLLR
CLTEVAFRTLGQSAAAGARPILFAAAGAEAVDGGYYGPGGRGERRGRVTDARVPAQARNLPVARRLWAVSERLTGVALS

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=306, Percent_Identity=37.9084967320261, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI186928839, Length=308, Percent_Identity=38.961038961039, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI193804850, Length=309, Percent_Identity=36.2459546925566, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI166795268, Length=307, Percent_Identity=35.5048859934853, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI10190746, Length=318, Percent_Identity=32.7044025157233, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI7706523, Length=312, Percent_Identity=35.2564102564103, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI19923931, Length=269, Percent_Identity=37.1747211895911, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI146231950, Length=308, Percent_Identity=36.038961038961, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI20149619, Length=246, Percent_Identity=30.8943089430894, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17532805, Length=313, Percent_Identity=29.7124600638978, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558006, Length=266, Percent_Identity=30.0751879699248, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17505416, Length=324, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI193207620, Length=324, Percent_Identity=28.0864197530864, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI72001364, Length=271, Percent_Identity=30.6273062730627, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17532791, Length=312, Percent_Identity=26.2820512820513, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115533172, Length=301, Percent_Identity=27.5747508305648, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115533174, Length=301, Percent_Identity=27.5747508305648, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535059, Length=313, Percent_Identity=27.7955271565495, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71992402, Length=313, Percent_Identity=25.8785942492013, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32565532, Length=143, Percent_Identity=38.4615384615385, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324820, Length=310, Percent_Identity=25.8064516129032, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921754, Length=310, Percent_Identity=36.4516129032258, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45554248, Length=306, Percent_Identity=36.6013071895425, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640117, Length=306, Percent_Identity=36.6013071895425, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI22024069, Length=317, Percent_Identity=34.0694006309148, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161076371, Length=312, Percent_Identity=34.6153846153846, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28573296, Length=306, Percent_Identity=33.9869281045752, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24586328, Length=307, Percent_Identity=32.5732899022801, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129347, Length=312, Percent_Identity=30.1282051282051, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656154, Length=300, Percent_Identity=31.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922628, Length=300, Percent_Identity=31.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45553409, Length=265, Percent_Identity=30.9433962264151, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24647948, Length=265, Percent_Identity=30.9433962264151, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24647946, Length=265, Percent_Identity=30.9433962264151, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24647931, Length=181, Percent_Identity=38.6740331491713, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 33788; Mature: 33657

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPLKWTIDRAPDLTGRVAVVTGATGGLGYETACGLASRGATVILTGRNADRGAAALAG
CCCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHHHH
LRTRVADARAEFMVLDLASLRSIADFAGDLTERLKGQGPGAVDILVNNAGVMAPPRRQET
HHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
EDGFELQFGTNYLGHFALTGRLRPLLVRAPGGARVVTVASLAARQGHITFDDLQARHRYS
CCCEEEEECCCCHHHEEEECCCCEEEEECCCCCEEEEEEHHHHHCCCCCHHHHHHHHCCC
PFGAYQQSKLANLIFALELDRLAQSGGWKLHSIAAHPGWSQTDLAISRSAARQGVAERLL
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RCLTEVAFRTLGQSAAAGARPILFAAAGAEAVDGGYYGPGGRGERRGRVTDARVPAQARN
HHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
LPVARRLWAVSERLTGVALS
CCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVPLKWTIDRAPDLTGRVAVVTGATGGLGYETACGLASRGATVILTGRNADRGAAALAG
CCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHHHH
LRTRVADARAEFMVLDLASLRSIADFAGDLTERLKGQGPGAVDILVNNAGVMAPPRRQET
HHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
EDGFELQFGTNYLGHFALTGRLRPLLVRAPGGARVVTVASLAARQGHITFDDLQARHRYS
CCCEEEEECCCCHHHEEEECCCCEEEEECCCCCEEEEEEHHHHHCCCCCHHHHHHHHCCC
PFGAYQQSKLANLIFALELDRLAQSGGWKLHSIAAHPGWSQTDLAISRSAARQGVAERLL
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RCLTEVAFRTLGQSAAAGARPILFAAAGAEAVDGGYYGPGGRGERRGRVTDARVPAQARN
HHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
LPVARRLWAVSERLTGVALS
CCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]