Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 162147024

GI number: 162147024

Start: 1221717

End: 1223024

Strand: Reverse

Name: trmE

Synonym: GDI_1229

Alternate gene names: 162147024

Gene position: 1223024-1221717 (Counterclockwise)

Preceding gene: 162147027

Following gene: 162147023

Centisome position: 31.01

GC content: 71.48

Gene sequence:

>1308_bases
ATGCCGACCGAACAGATGCCGATCTTCGCGCTGGCGACCGGGGCGGGACGCGCGGCCATCGCCGTCATGCGCCTGACCGG
GGCGGGATGCGGCGACATGCTGCAACGGCTGTGCGGCCCGCTGCCGCCGCCGCGCCAGGCATCCCTGCGTGGCCTGTGGC
GGCGTGACGCGGCGGCGGCGCCGGTGCTGCTGGACCGGGCGCTGGTGCTGTGGTTCCCCGGTCCGCGCAGCTATACCGGC
GAGGACAGCGCGGAGCTGCACCTGCATGCCGGCCCGGCGGTGATCGCGGGCGTGGCCGACGCGCTGGTGGCACTGGGCGC
ACGGCCGGCGGAGCCGGGCGAATTCACCCGCCGTGCCTTCGCCCATGGCCGACTGGACCTGATCGAGGCCGAGGGCATCG
CCGACCTGATCGATGCCGAGACCGAAGCGCAGCGGCGGCAGGCCCTGGACCAGGCGGACGGGACGCTGAGCCGGATCTAT
GACGGCTGGGCGGCCCGGCTGCGGACCCTGCTGGCACATCAGGAGGCACTGATCGACTTCCCGGACGAGGACCTGCCGCC
CGGTGTGGAACAGGCGCTGCTGGATGACCTGACGGCGCTGCGGTCCGAGATGCAGGCCCACCTGGACGATGGCGGCCGGG
GCGAGAAGCTGCGCAGGGGGCTGGTCTTCACCATTGTCGGGGCGCCCAATGTCGGCAAGTCCAGCCTGCTGAACGCGCTG
GCCGGGCGGGACGCGGCCATTGTTTCGGCCATTGCAGGGACCACGCGCGATGCCATCGAAATCCGCGTGGTGCTGGGCGA
TGTGCCCGTGACCCTGATCGACACCGCCGGCCTGCGCGAGACACAGGACGAAATCGAGGCCGAGGGCGTAAGGCGGGCCC
TGTTTCACGTGAAACATGCCGACTGCGTGATCGCGATGTTCGACGGCGAGACGGTGCCCGACGATATTCCGGCGGATGCG
ATCAGGGTGCGGAACAAGGTCGACCTGGCACCGGTGCCCGCCGGGGCGGATGCGATCGGGATCAGCGTCCGGCAGGGCAC
GGGCATGGACGCGCTGCGCACGGCGCTGGCCGAACGCGCCCGGGCGCTGACCGCGTCAGCGGCCGGCCCGCCGCTGACGC
GGGCCCGCCATCGGGCGGCGATCGAAGAAACCGCTGGCCATCTGGCCGCCGCGTTGGATATGCACTGGCCAGAGATGCGG
GGCGAGGAAATGCGCCTGGCCATGCGCGCCCTGGGGCGCCTGACCGGGGCGGTAGGGGTGGAGGATCTGCTCGATACGGT
GTTCGGGCAGTTCTGTATCGGGAAGTGA

Upstream 100 bases:

>100_bases
ACGCGCATATCGGTTCGGTCGCGTATTCTGTACTCTTCCGCCGCAGGATGCGACATGCAAGAGGGATCACGCACACGCCG
GGACAACGGGGACAGGACAG

Downstream 100 bases:

>100_bases
AGGGGTGTTCTTTTTCTTCAGAAAAAGAAGCACTTAAACGGGGGCAGGCAAACGTGACGACCGAGTACGATGTCATCGTC
ATAGGCGGAGGCCACGCAGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPTEQMPIFALATGAGRAAIAVMRLTGAGCGDMLQRLCGPLPPPRQASLRGLWRRDAAAAPVLLDRALVLWFPGPRSYTG
EDSAELHLHAGPAVIAGVADALVALGARPAEPGEFTRRAFAHGRLDLIEAEGIADLIDAETEAQRRQALDQADGTLSRIY
DGWAARLRTLLAHQEALIDFPDEDLPPGVEQALLDDLTALRSEMQAHLDDGGRGEKLRRGLVFTIVGAPNVGKSSLLNAL
AGRDAAIVSAIAGTTRDAIEIRVVLGDVPVTLIDTAGLRETQDEIEAEGVRRALFHVKHADCVIAMFDGETVPDDIPADA
IRVRNKVDLAPVPAGADAIGISVRQGTGMDALRTALAERARALTASAAGPPLTRARHRAAIEETAGHLAAALDMHWPEMR
GEEMRLAMRALGRLTGAVGVEDLLDTVFGQFCIGK

Sequences:

>Translated_435_residues
MPTEQMPIFALATGAGRAAIAVMRLTGAGCGDMLQRLCGPLPPPRQASLRGLWRRDAAAAPVLLDRALVLWFPGPRSYTG
EDSAELHLHAGPAVIAGVADALVALGARPAEPGEFTRRAFAHGRLDLIEAEGIADLIDAETEAQRRQALDQADGTLSRIY
DGWAARLRTLLAHQEALIDFPDEDLPPGVEQALLDDLTALRSEMQAHLDDGGRGEKLRRGLVFTIVGAPNVGKSSLLNAL
AGRDAAIVSAIAGTTRDAIEIRVVLGDVPVTLIDTAGLRETQDEIEAEGVRRALFHVKHADCVIAMFDGETVPDDIPADA
IRVRNKVDLAPVPAGADAIGISVRQGTGMDALRTALAERARALTASAAGPPLTRARHRAAIEETAGHLAAALDMHWPEMR
GEEMRLAMRALGRLTGAVGVEDLLDTVFGQFCIGK
>Mature_434_residues
PTEQMPIFALATGAGRAAIAVMRLTGAGCGDMLQRLCGPLPPPRQASLRGLWRRDAAAAPVLLDRALVLWFPGPRSYTGE
DSAELHLHAGPAVIAGVADALVALGARPAEPGEFTRRAFAHGRLDLIEAEGIADLIDAETEAQRRQALDQADGTLSRIYD
GWAARLRTLLAHQEALIDFPDEDLPPGVEQALLDDLTALRSEMQAHLDDGGRGEKLRRGLVFTIVGAPNVGKSSLLNALA
GRDAAIVSAIAGTTRDAIEIRVVLGDVPVTLIDTAGLRETQDEIEAEGVRRALFHVKHADCVIAMFDGETVPDDIPADAI
RVRNKVDLAPVPAGADAIGISVRQGTGMDALRTALAERARALTASAAGPPLTRARHRAAIEETAGHLAAALDMHWPEMRG
EEMRLAMRALGRLTGAVGVEDLLDTVFGQFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=44.136460554371, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI193082993, Length=449, Percent_Identity=45.43429844098, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI193082988, Length=504, Percent_Identity=40.8730158730159, Blast_Score=300, Evalue=3e-81,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=39.6929824561403, Blast_Score=267, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI17507259, Length=455, Percent_Identity=32.967032967033, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=32.1285140562249, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=38.9830508474576, Blast_Score=292, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_GLUDA (A9HE16)

Other databases:

- EMBL:   CP001189
- EMBL:   AM889285
- RefSeq:   YP_001601485.1
- RefSeq:   YP_002276315.1
- ProteinModelPortal:   A9HE16
- SMR:   A9HE16
- GeneID:   5788762
- GeneID:   6975366
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1940
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 46133; Mature: 46002

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 85-85 BINDING 124-124 BINDING 435-435

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEQMPIFALATGAGRAAIAVMRLTGAGCGDMLQRLCGPLPPPRQASLRGLWRRDAAAA
CCCCCCCEEEEECCCCHHHEEEEHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
PVLLDRALVLWFPGPRSYTGEDSAELHLHAGPAVIAGVADALVALGARPAEPGEFTRRAF
HHHHHCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AHGRLDLIEAEGIADLIDAETEAQRRQALDQADGTLSRIYDGWAARLRTLLAHQEALIDF
HCCCEEEEECCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDEDLPPGVEQALLDDLTALRSEMQAHLDDGGRGEKLRRGLVFTIVGAPNVGKSSLLNAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHH
AGRDAAIVSAIAGTTRDAIEIRVVLGDVPVTLIDTAGLRETQDEIEAEGVRRALFHVKHA
HCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DCVIAMFDGETVPDDIPADAIRVRNKVDLAPVPAGADAIGISVRQGTGMDALRTALAERA
CEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RALTASAAGPPLTRARHRAAIEETAGHLAAALDMHWPEMRGEEMRLAMRALGRLTGAVGV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCH
EDLLDTVFGQFCIGK
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTEQMPIFALATGAGRAAIAVMRLTGAGCGDMLQRLCGPLPPPRQASLRGLWRRDAAAA
CCCCCCEEEEECCCCHHHEEEEHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
PVLLDRALVLWFPGPRSYTGEDSAELHLHAGPAVIAGVADALVALGARPAEPGEFTRRAF
HHHHHCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AHGRLDLIEAEGIADLIDAETEAQRRQALDQADGTLSRIYDGWAARLRTLLAHQEALIDF
HCCCEEEEECCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDEDLPPGVEQALLDDLTALRSEMQAHLDDGGRGEKLRRGLVFTIVGAPNVGKSSLLNAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHH
AGRDAAIVSAIAGTTRDAIEIRVVLGDVPVTLIDTAGLRETQDEIEAEGVRRALFHVKHA
HCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DCVIAMFDGETVPDDIPADAIRVRNKVDLAPVPAGADAIGISVRQGTGMDALRTALAERA
CEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RALTASAAGPPLTRARHRAAIEETAGHLAAALDMHWPEMRGEEMRLAMRALGRLTGAVGV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCH
EDLLDTVFGQFCIGK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA