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The map label for this gene is trpI [H]

Identifier: 162146777

GI number: 162146777

Start: 976242

End: 977099

Strand: Direct

Name: trpI [H]

Synonym: GDI_0959

Alternate gene names: 162146777

Gene position: 976242-977099 (Clockwise)

Preceding gene: 162146774

Following gene: 162146778

Centisome position: 24.75

GC content: 64.1

Gene sequence:

>858_bases
ATGAAGCGATCAGACATCCCGTCTCTGGACGATCTGCGTGCGTTCGAAGCGGTAACGCGCCTCGGTTCCGTGCGAGCGGC
TGCCATCGAACTGGCTCTGACGCATGGAGCGATCAGCCGCCGGGTGTCCAAGCTGGCTGCTGACCTCAAACTGCAACTAT
TGGAACCCGACGGTCGTGGCGTACGGCCGACCCCGGATGGTGAACGCCTGGCGCTGGCCACGAGCGATGCACTGAAGGGA
ATATCGACAGCGCTCGCCGAGATCAGGTCGCCTCTGACCTCCCCGCCGATCGTGCTGTCCTGCGAACGATCGCTGGCGAT
GCGCTGGCTGATCCCGCGCCTGTCGGATTTTCAGGATCGTCACCCCGCCATCGACGTGCATCTCTCGACCGGCGGCGGTC
CGCTGGATTTCGAGCGGGAACGCATCACCCTAGCAATCCGACGGCTCGACTTCCCGCTGGATCCGGACTGGACCATAACG
ACGCTCACAGCAGAGGCGGTCGGTCCGGTCATGCGACCTGATATGGCGGGGCGATTCGAGGACGGAGATTACCTGGCGCT
CGGCTCGCGGACCCGCCCCGAGGCATGGCCGGAATGGCTGGCCACACGGCCCGACGTCCAAGCGCCACGCGACATCCGTC
TCTACGACCATCATTTCCTGATGCTAGAGGCTGCCGCCAGCGGCTTGGGTGTCGCCCTCTCGCCCCGGATCATCGCGGCG
GACGATGTCGCGAACGGACGGCTGATGGCCCCGGTGGGCTTCCAGGCGGACGGAACGGGTTACGGATTGATCAGACCCAG
CAGAATGGAGGTGACGGACCACGTCGAGGCCCTAGAAAAATGGATCAAGAAACAGTAG

Upstream 100 bases:

>100_bases
GACTGAAGGAAGGTGTTTCGCTCATGATGGTGACAATAACGGAACAGGGTCCACAGAAAATCGATATAAACTGACCTCTT
CTGGTGATAAAAACTGACCA

Downstream 100 bases:

>100_bases
AATTAATAAATAGTATATATAGTTTATGGAAACATATCCATAATATATAATTTTGAAAAACAAACCGATAAAAATTCAAA
TCTATTTCTATTACTTATTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKRSDIPSLDDLRAFEAVTRLGSVRAAAIELALTHGAISRRVSKLAADLKLQLLEPDGRGVRPTPDGERLALATSDALKG
ISTALAEIRSPLTSPPIVLSCERSLAMRWLIPRLSDFQDRHPAIDVHLSTGGGPLDFERERITLAIRRLDFPLDPDWTIT
TLTAEAVGPVMRPDMAGRFEDGDYLALGSRTRPEAWPEWLATRPDVQAPRDIRLYDHHFLMLEAAASGLGVALSPRIIAA
DDVANGRLMAPVGFQADGTGYGLIRPSRMEVTDHVEALEKWIKKQ

Sequences:

>Translated_285_residues
MKRSDIPSLDDLRAFEAVTRLGSVRAAAIELALTHGAISRRVSKLAADLKLQLLEPDGRGVRPTPDGERLALATSDALKG
ISTALAEIRSPLTSPPIVLSCERSLAMRWLIPRLSDFQDRHPAIDVHLSTGGGPLDFERERITLAIRRLDFPLDPDWTIT
TLTAEAVGPVMRPDMAGRFEDGDYLALGSRTRPEAWPEWLATRPDVQAPRDIRLYDHHFLMLEAAASGLGVALSPRIIAA
DDVANGRLMAPVGFQADGTGYGLIRPSRMEVTDHVEALEKWIKKQ
>Mature_285_residues
MKRSDIPSLDDLRAFEAVTRLGSVRAAAIELALTHGAISRRVSKLAADLKLQLLEPDGRGVRPTPDGERLALATSDALKG
ISTALAEIRSPLTSPPIVLSCERSLAMRWLIPRLSDFQDRHPAIDVHLSTGGGPLDFERERITLAIRRLDFPLDPDWTIT
TLTAEAVGPVMRPDMAGRFEDGDYLALGSRTRPEAWPEWLATRPDVQAPRDIRLYDHHFLMLEAAASGLGVALSPRIIAA
DDVANGRLMAPVGFQADGTGYGLIRPSRMEVTDHVEALEKWIKKQ

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=27.6595744680851, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=27.5510204081633, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789173, Length=301, Percent_Identity=26.578073089701, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31290; Mature: 31290

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSDIPSLDDLRAFEAVTRLGSVRAAAIELALTHGAISRRVSKLAADLKLQLLEPDGRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
VRPTPDGERLALATSDALKGISTALAEIRSPLTSPPIVLSCERSLAMRWLIPRLSDFQDR
CCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
HPAIDVHLSTGGGPLDFERERITLAIRRLDFPLDPDWTITTLTAEAVGPVMRPDMAGRFE
CCEEEEEEECCCCCCCCCHHHEEEEEEHHCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCC
DGDYLALGSRTRPEAWPEWLATRPDVQAPRDIRLYDHHFLMLEAAASGLGVALSPRIIAA
CCCEEEECCCCCCCCCHHHHCCCCCCCCCCCEEEECCEEEEEEECCCCCCEEECCCEEEE
DDVANGRLMAPVGFQADGTGYGLIRPSRMEVTDHVEALEKWIKKQ
CCCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRSDIPSLDDLRAFEAVTRLGSVRAAAIELALTHGAISRRVSKLAADLKLQLLEPDGRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
VRPTPDGERLALATSDALKGISTALAEIRSPLTSPPIVLSCERSLAMRWLIPRLSDFQDR
CCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
HPAIDVHLSTGGGPLDFERERITLAIRRLDFPLDPDWTITTLTAEAVGPVMRPDMAGRFE
CCEEEEEEECCCCCCCCCHHHEEEEEEHHCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCC
DGDYLALGSRTRPEAWPEWLATRPDVQAPRDIRLYDHHFLMLEAAASGLGVALSPRIIAA
CCCEEEECCCCCCCCCHHHHCCCCCCCCCCCEEEECCEEEEEEECCCCCCEEECCCEEEE
DDVANGRLMAPVGFQADGTGYGLIRPSRMEVTDHVEALEKWIKKQ
CCCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]