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The map label for this gene is tgt

Identifier: 162146699

GI number: 162146699

Start: 893359

End: 894522

Strand: Direct

Name: tgt

Synonym: GDI_0877

Alternate gene names: 162146699

Gene position: 893359-894522 (Clockwise)

Preceding gene: 162146698

Following gene: 162146700

Centisome position: 22.65

GC content: 69.42

Gene sequence:

>1164_bases
ATGAGCACCGCATTCCGCTGGGTGGAACAGGCCCGGGTGGGCCGGGCGCGGGCGGGGCACCTGCATACCGCACACGGCGT
GGTCCCCACCCCCACCTTCATGCCCGTGGGCACCGTGGGCACCGTCAAGGCGATGACGATGGATTCCGTGCGCTCCACGG
GGGCGGGCATCGTGCTGGGCAACACCTATCACCTGATGCTGCGTCCGGGGGCGGAGAAGGTCCGCGCCCTGGGCGGCCTG
CACCGCTTCATGGATTGGCCGGGGCCGATCCTGACCGATTCCGGCGGGTTCCAGGTCATGTCGCTGGGCGCGTTGCGCAA
GCTGGACCAGGACGGGGTGACGTTCAATTCGCACATCGACGGGTCCAAGCACCGGCTGACGCCCGAACGATCGACCGACA
TCCAGCATGCGCTGGATGCCACCATCACCATGTGCTTCGACGAATGCCCGGCCCTGCCGGCACCGCCCGAGACGATCGCG
CAATCCATGCGGCTGTCCATGCGCTGGGCCGCCCGCTGCCGCGAAGCCTTCGTGCCGCGCGCGGGCTATGCCCAATACGG
CATCATCCAGGGCGGGACCGAACCCGAATTGCGGGCCGAGAGCGTGCGCGCCCTGACCGGGATCGGCTTCGAAGGCTACG
CCATCGGCGGCTTGGCGGTGGGCGAGGGGCAGGAGCTGATGTACGCGACGCTGGATGCGACCGTGCCGCTGATCCCGCAC
GACAGCCCGCGCTACCTGATGGGGGTCGGCACGCCGGACGACCTGCTGGGGGCGGTCGAACGCGGGGTGGATATGTTCGA
CTGCGTCATGCCCACCCGGGCGGGACGCACCGCCCGCGCCTATACCGAACGCGGCACGCTGAACCTGCGCAACGCCCGCC
ATGCCGACGATACCCGTCCGCTTTCGCCCCATTGCGACTGCTTGGCCTGCACGCGGCACAGCCGGGCCTATCTGCACCAC
CTGTTCCGTGCGAACGAAATCCTGGGTCCGATGCTGCTGACCTGGCACAATCTGGCCTATTACCAGCGGCTGATGCGCGG
CATGCGCGGCGCGATCGTCGCGGGCACGCTGGGTGCCCACGCCGCCGGATTGCGCGCCGAATGGGCCATGGAAGACTGGA
CGCCCGACGAGATGCCGCCCCCCGACCTTCCCCCGGTCCCGTGA

Upstream 100 bases:

>100_bases
GTGACGCCTACGCCCATGCCGTCGCCGACGGCTACCGGTTCTATTCCTACGGCGATGCCTGCCTGCTGCATCGCGCGCCG
GTCGCCGGGGAGCAGGGACT

Downstream 100 bases:

>100_bases
TGGAGGGCGGCCTGCCGCCCGGAAGAGCTGCCATCCTGCGCGATGCGATTCGCGACAGGGCCCTGGCGCTGGGGTTCGAT
GCGGTGGGCTTCTGCGAGGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSTAFRWVEQARVGRARAGHLHTAHGVVPTPTFMPVGTVGTVKAMTMDSVRSTGAGIVLGNTYHLMLRPGAEKVRALGGL
HRFMDWPGPILTDSGGFQVMSLGALRKLDQDGVTFNSHIDGSKHRLTPERSTDIQHALDATITMCFDECPALPAPPETIA
QSMRLSMRWAARCREAFVPRAGYAQYGIIQGGTEPELRAESVRALTGIGFEGYAIGGLAVGEGQELMYATLDATVPLIPH
DSPRYLMGVGTPDDLLGAVERGVDMFDCVMPTRAGRTARAYTERGTLNLRNARHADDTRPLSPHCDCLACTRHSRAYLHH
LFRANEILGPMLLTWHNLAYYQRLMRGMRGAIVAGTLGAHAAGLRAEWAMEDWTPDEMPPPDLPPVP

Sequences:

>Translated_387_residues
MSTAFRWVEQARVGRARAGHLHTAHGVVPTPTFMPVGTVGTVKAMTMDSVRSTGAGIVLGNTYHLMLRPGAEKVRALGGL
HRFMDWPGPILTDSGGFQVMSLGALRKLDQDGVTFNSHIDGSKHRLTPERSTDIQHALDATITMCFDECPALPAPPETIA
QSMRLSMRWAARCREAFVPRAGYAQYGIIQGGTEPELRAESVRALTGIGFEGYAIGGLAVGEGQELMYATLDATVPLIPH
DSPRYLMGVGTPDDLLGAVERGVDMFDCVMPTRAGRTARAYTERGTLNLRNARHADDTRPLSPHCDCLACTRHSRAYLHH
LFRANEILGPMLLTWHNLAYYQRLMRGMRGAIVAGTLGAHAAGLRAEWAMEDWTPDEMPPPDLPPVP
>Mature_386_residues
STAFRWVEQARVGRARAGHLHTAHGVVPTPTFMPVGTVGTVKAMTMDSVRSTGAGIVLGNTYHLMLRPGAEKVRALGGLH
RFMDWPGPILTDSGGFQVMSLGALRKLDQDGVTFNSHIDGSKHRLTPERSTDIQHALDATITMCFDECPALPAPPETIAQ
SMRLSMRWAARCREAFVPRAGYAQYGIIQGGTEPELRAESVRALTGIGFEGYAIGGLAVGEGQELMYATLDATVPLIPHD
SPRYLMGVGTPDDLLGAVERGVDMFDCVMPTRAGRTARAYTERGTLNLRNARHADDTRPLSPHCDCLACTRHSRAYLHHL
FRANEILGPMLLTWHNLAYYQRLMRGMRGAIVAGTLGAHAAGLRAEWAMEDWTPDEMPPPDLPPVP

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=348, Percent_Identity=42.5287356321839, Blast_Score=281, Evalue=1e-75,
Organism=Escherichia coli, GI1786607, Length=345, Percent_Identity=55.3623188405797, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=41.2429378531073, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI25146502, Length=154, Percent_Identity=26.6233766233766, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920510, Length=348, Percent_Identity=43.6781609195402, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI21355311, Length=195, Percent_Identity=29.7435897435897, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_GLUDA (A9HBJ2)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601158.1
- RefSeq:   YP_002275540.1
- ProteinModelPortal:   A9HBJ2
- SMR:   A9HBJ2
- GeneID:   5792322
- GeneID:   6974546
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1142
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42199; Mature: 42068

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAFRWVEQARVGRARAGHLHTAHGVVPTPTFMPVGTVGTVKAMTMDSVRSTGAGIVLG
CCHHHHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEC
NTYHLMLRPGAEKVRALGGLHRFMDWPGPILTDSGGFQVMSLGALRKLDQDGVTFNSHID
CEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHCCCCCEEECCCC
GSKHRLTPERSTDIQHALDATITMCFDECPALPAPPETIAQSMRLSMRWAARCREAFVPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AGYAQYGIIQGGTEPELRAESVRALTGIGFEGYAIGGLAVGEGQELMYATLDATVPLIPH
CCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEECCEEECCCCEEEEEEECCEEECCCC
DSPRYLMGVGTPDDLLGAVERGVDMFDCVMPTRAGRTARAYTERGTLNLRNARHADDTRP
CCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHCCCCEEECCCCCCCCCCC
LSPHCDCLACTRHSRAYLHHLFRANEILGPMLLTWHNLAYYQRLMRGMRGAIVAGTLGAH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
AAGLRAEWAMEDWTPDEMPPPDLPPVP
HCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STAFRWVEQARVGRARAGHLHTAHGVVPTPTFMPVGTVGTVKAMTMDSVRSTGAGIVLG
CHHHHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEC
NTYHLMLRPGAEKVRALGGLHRFMDWPGPILTDSGGFQVMSLGALRKLDQDGVTFNSHID
CEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHCCCCCEEECCCC
GSKHRLTPERSTDIQHALDATITMCFDECPALPAPPETIAQSMRLSMRWAARCREAFVPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AGYAQYGIIQGGTEPELRAESVRALTGIGFEGYAIGGLAVGEGQELMYATLDATVPLIPH
CCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEECCEEECCCCEEEEEEECCEEECCCC
DSPRYLMGVGTPDDLLGAVERGVDMFDCVMPTRAGRTARAYTERGTLNLRNARHADDTRP
CCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHCCCCEEECCCCCCCCCCC
LSPHCDCLACTRHSRAYLHHLFRANEILGPMLLTWHNLAYYQRLMRGMRGAIVAGTLGAH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
AAGLRAEWAMEDWTPDEMPPPDLPPVP
HCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA