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The map label for this gene is queA

Identifier: 162146698

GI number: 162146698

Start: 892295

End: 893362

Strand: Direct

Name: queA

Synonym: GDI_0876

Alternate gene names: 162146698

Gene position: 892295-893362 (Clockwise)

Preceding gene: 162146694

Following gene: 162146699

Centisome position: 22.62

GC content: 71.44

Gene sequence:

>1068_bases
ATGACCGACCTTGCCTCCGATTTCGATTTCGACCTGCCGCCCGCGCTGATCGCCGAGCATCCGGCGCGGCCGCGCGATTC
CGCGCGGCTGCTGGACATTCCGGCCGCCGGCCCGTTCCATGACCGCATCGTGCGCGACCTGCCGTCGCTGCTGCGGCCCG
GCGACCTGCTGGTGGCCAACGATACGGCGGTCATCCCGGCGCAACTGGACGCCCGCCGGGGCGAGGCACGGATCGGCATC
ACGCTGGACCGCATCCTGCCGGACGGGACATGGCATGCGCTGGCCCGCAACGCCCGCAGGCTGCGGGCCGGCGACGTGCT
GACCTTTCCGGGCACGCCCAATACCGCCGCCGTCATCTCGCGCGATGACGAGGGCGGGGTGGTCCTGCGCTTCGATACCG
AGGGTGCGGCCTTCGACGCCTTCCTGCAGGCGGCGGGCGTCCTGGCGCTGCCGCCCTATATCGCACGGCCCTTCGGCCCG
ACCGAACAGGACCGCGTCGATTACGCCACCATCTTCTCGGCCCATCGCGGCGCGGTGGCGGCCCCTACGGCGGGGCTGCA
TTTCACGCCGGACCTGCTGGCGGCGCTGGACGCGGCGGGGGTGGGGCGGTGTACCCTCACCCTGCATGTCGGGGCCGGGA
CGTTCCTGCCCGTGCGGGGGGACAGCATCGCCGCCCACCGCATGCATGCCGAACGCGGCGTGATCACGGCCGAGACCGCC
GATGCGATCAACGCGGCGCGCCGGGCGGGCGGGCGCATCGTCGCGGTCGGCACGACGTCGCTGCGCCTGCTGGAAAGCGC
CGCCGATCCGGACGGCACGATCCGCCCCTTCGACGGCGACACCGCGATCTTCATCCGCCCGGGCTACCGCTTCCGGGCGG
TGGACGTGCTGATGACCAATTTCCATCTGCCGCGCTCGACCCTGTTCATGCTGGTCTGCGCGTTTGCCGGATATGACAGG
ATGCGTGACGCCTACGCCCATGCCGTCGCCGACGGCTACCGGTTCTATTCCTACGGCGATGCCTGCCTGCTGCATCGCGC
GCCGGTCGCCGGGGAGCAGGGACTATGA

Upstream 100 bases:

>100_bases
ATTTTGACGGGTGGCGCGGCACCTGTCCAACGGGGCCTGTCCGGCGGGGCCGCGTGAGATGCTGGCCACGCGGCCCGGGA
TTCCGTAAACGGGGCATCCG

Downstream 100 bases:

>100_bases
GCACCGCATTCCGCTGGGTGGAACAGGCCCGGGTGGGCCGGGCGCGGGCGGGGCACCTGCATACCGCACACGGCGTGGTC
CCCACCCCCACCTTCATGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTDLASDFDFDLPPALIAEHPARPRDSARLLDIPAAGPFHDRIVRDLPSLLRPGDLLVANDTAVIPAQLDARRGEARIGI
TLDRILPDGTWHALARNARRLRAGDVLTFPGTPNTAAVISRDDEGGVVLRFDTEGAAFDAFLQAAGVLALPPYIARPFGP
TEQDRVDYATIFSAHRGAVAAPTAGLHFTPDLLAALDAAGVGRCTLTLHVGAGTFLPVRGDSIAAHRMHAERGVITAETA
DAINAARRAGGRIVAVGTTSLRLLESAADPDGTIRPFDGDTAIFIRPGYRFRAVDVLMTNFHLPRSTLFMLVCAFAGYDR
MRDAYAHAVADGYRFYSYGDACLLHRAPVAGEQGL

Sequences:

>Translated_355_residues
MTDLASDFDFDLPPALIAEHPARPRDSARLLDIPAAGPFHDRIVRDLPSLLRPGDLLVANDTAVIPAQLDARRGEARIGI
TLDRILPDGTWHALARNARRLRAGDVLTFPGTPNTAAVISRDDEGGVVLRFDTEGAAFDAFLQAAGVLALPPYIARPFGP
TEQDRVDYATIFSAHRGAVAAPTAGLHFTPDLLAALDAAGVGRCTLTLHVGAGTFLPVRGDSIAAHRMHAERGVITAETA
DAINAARRAGGRIVAVGTTSLRLLESAADPDGTIRPFDGDTAIFIRPGYRFRAVDVLMTNFHLPRSTLFMLVCAFAGYDR
MRDAYAHAVADGYRFYSYGDACLLHRAPVAGEQGL
>Mature_354_residues
TDLASDFDFDLPPALIAEHPARPRDSARLLDIPAAGPFHDRIVRDLPSLLRPGDLLVANDTAVIPAQLDARRGEARIGIT
LDRILPDGTWHALARNARRLRAGDVLTFPGTPNTAAVISRDDEGGVVLRFDTEGAAFDAFLQAAGVLALPPYIARPFGPT
EQDRVDYATIFSAHRGAVAAPTAGLHFTPDLLAALDAAGVGRCTLTLHVGAGTFLPVRGDSIAAHRMHAERGVITAETAD
AINAARRAGGRIVAVGTTSLRLLESAADPDGTIRPFDGDTAIFIRPGYRFRAVDVLMTNFHLPRSTLFMLVCAFAGYDRM
RDAYAHAVADGYRFYSYGDACLLHRAPVAGEQGL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=349, Percent_Identity=44.6991404011461, Blast_Score=261, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_GLUDA (A9HBI8)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601157.1
- RefSeq:   YP_002275541.1
- ProteinModelPortal:   A9HBI8
- SMR:   A9HBI8
- GeneID:   5792321
- GeneID:   6974547
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1143
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37981; Mature: 37849

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLASDFDFDLPPALIAEHPARPRDSARLLDIPAAGPFHDRIVRDLPSLLRPGDLLVAN
CCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEC
DTAVIPAQLDARRGEARIGITLDRILPDGTWHALARNARRLRAGDVLTFPGTPNTAAVIS
CCEEEECCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHEECCCEEECCCCCCCEEEEE
RDDEGGVVLRFDTEGAAFDAFLQAAGVLALPPYIARPFGPTEQDRVDYATIFSAHRGAVA
ECCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHCCCCCCCHHCCHHHHHHHHCCCCEE
APTAGLHFTPDLLAALDAAGVGRCTLTLHVGAGTFLPVRGDSIAAHRMHAERGVITAETA
CCCCCCEECHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHCCEEEECHH
DAINAARRAGGRIVAVGTTSLRLLESAADPDGTIRPFDGDTAIFIRPGYRFRAVDVLMTN
HHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCEEECCCCEEEEEECCCEEEEEEEEECC
FHLPRSTLFMLVCAFAGYDRMRDAYAHAVADGYRFYSYGDACLLHRAPVAGEQGL
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCC
>Mature Secondary Structure 
TDLASDFDFDLPPALIAEHPARPRDSARLLDIPAAGPFHDRIVRDLPSLLRPGDLLVAN
CCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEC
DTAVIPAQLDARRGEARIGITLDRILPDGTWHALARNARRLRAGDVLTFPGTPNTAAVIS
CCEEEECCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHEECCCEEECCCCCCCEEEEE
RDDEGGVVLRFDTEGAAFDAFLQAAGVLALPPYIARPFGPTEQDRVDYATIFSAHRGAVA
ECCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHCCCCCCCHHCCHHHHHHHHCCCCEE
APTAGLHFTPDLLAALDAAGVGRCTLTLHVGAGTFLPVRGDSIAAHRMHAERGVITAETA
CCCCCCEECHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHCCEEEECHH
DAINAARRAGGRIVAVGTTSLRLLESAADPDGTIRPFDGDTAIFIRPGYRFRAVDVLMTN
HHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCEEECCCCEEEEEECCCEEEEEEEEECC
FHLPRSTLFMLVCAFAGYDRMRDAYAHAVADGYRFYSYGDACLLHRAPVAGEQGL
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA