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The map label for this gene is nuoM [H]

Identifier: 162146418

GI number: 162146418

Start: 598799

End: 600373

Strand: Direct

Name: nuoM [H]

Synonym: GDI_0593

Alternate gene names: 162146418

Gene position: 598799-600373 (Clockwise)

Preceding gene: 162146417

Following gene: 162146419

Centisome position: 15.18

GC content: 67.17

Gene sequence:

>1575_bases
GTGGGCTGGTCTCGCCGTCCGAACGGGGATAGGCTTCCGCCATCTTTCACGGACGATGAGACCGACGTGCATCCGATCCT
GCTCCCCCTCGTCACGTACCTGCCCCTCGGCGGTGCGCTTGCCATCCTCCTGATGCGCGGCACGTCCGAGGCTGTCGAGG
GCGCGTCGCGCTGGGTGGCGCTGTGGACCAGCCTGATCGTCTTCGGCCTGTCGGTGGTGATGTGGGGCGAATTCGATCCG
TCCCTGCCGGGCTTCCAGTTCGTCCAGCAGGTCGGCTGGGCCTCGTCCTTCGGCATTTCGTACCATGCGGGCGTGGACGG
GATCAGCCTGCTGTTCGTCCTGCTGTCCACCTTCCTGACCCCGCTGGCGGTCATTGGCGGGTGGAAGCTGGTGACGACCC
GGGTGCGCGACTACATGCTGGCGGTGCTGCTGCTGGAAACCACGCTGGTCGGCCTGTTCTCGGCGCTGGACCTGGTGCTG
TTCTACGTCTTCTACGAGGCGACGCTGATTCCGGGCAGCCTGATGATCGGCGTATGGGGCGGCCCGAAGCGCGTCTGGGC
GTCGATCCAGTTCTTCCTGTTCACCTTCGGCGGCTCGCTGTTCATGCTGCTGGCGCTGCTGACGATGTGGAACATGACCG
GCACGACCGATATTCCCACGCTGATGCAGGCAACGTTCCCGCGCGCCATGCAGTGCTGGCTGCTGCTGGGCTTCGTCCTG
GCCTTCGGGGTGAAGCTGCCGCTGTTCCCGCTGCATGCCTGGCTGCCCGATGCCTATACCGAGGCCCCGACCCCGACCTC
GGCCCTGCTGTCGGGCGTGCTGTCCAAGGCCGGCGCCTACGGGCTGCTGCGGTTCGGCGTCCTGATGTTCCCCGAGGCGG
CTCGGCTGTTCGCCCCCTATATCCTGACGCTGGGCGTCATCGCGGTGATCTATGCCGCGATCATCGCCCTGGCCCAGACC
GACATGAAGCGGGTGATCGCCTATTCCTCGTTCTCGCACATGGGGGTGATCGCGGTCGGCCTGTTCACCCTGACACCCGA
AGGGATCGACGGCGCGATCTTCACCATGCTGTCCCACGGCGTGATCATCACCGCGCTGTTCTTCTGCGTCGCCGCTGTCT
CGTGGCGGGCGGAAACGCGGTCGATCGACGCGTTTTCCGGCGTGGCGCACAAGATGCCGGTGCTGGCGACGCTGGCGATG
CTGTTCGTCATGGCCAATATCGGCCTGCCGGGCACCGGGTCCTTCGTCGGCGAACTGCTGGTGATGGTCGGCGCGATCCA
CGTCTCGTTCTGGGTGGCCTTCCTGGCCGGCAGCACCATGATCCTGGGCGCGGTCTACATGCTGGTGCTGTATCGCCGGG
TCATGTTCGGCGCCGCCGGGCACGGGGTGGTGGCCATGCTGCGCGACCTGACCGGGCCGGAACTGGCGGTGCTGGTGCCC
TGTGCCCTGGTCACGCTGTGGATGGGCATCCACCCGACCAGCTTCCTGCGGGTCTTCGACCCGGCGGTGGTCCATGCCGT
CGCGGCGCCGGCCCAGGCCTCTGCCGCCGCGCCGATCCACCTCGCCGCACGCTGA

Upstream 100 bases:

>100_bases
AGCTGAGCCGCACGGACCTGGAAAAACTCTTCGGCCGCAAATAGTCCCATGCAGCCAATGCAAGGGAAATCGTACGTCCT
CGGCAGAACGTGAGCGGCGT

Downstream 100 bases:

>100_bases
ACCGTCCCAATCCTGATCGCTCCGAAACGGGCAGGCACGCCGTGCCTGCCCGTTTTGCTGTGGCGAAGCCTATTGTCCGG
CCGCCGCCCGTGCGGCATGA

Product: NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MGWSRRPNGDRLPPSFTDDETDVHPILLPLVTYLPLGGALAILLMRGTSEAVEGASRWVALWTSLIVFGLSVVMWGEFDP
SLPGFQFVQQVGWASSFGISYHAGVDGISLLFVLLSTFLTPLAVIGGWKLVTTRVRDYMLAVLLLETTLVGLFSALDLVL
FYVFYEATLIPGSLMIGVWGGPKRVWASIQFFLFTFGGSLFMLLALLTMWNMTGTTDIPTLMQATFPRAMQCWLLLGFVL
AFGVKLPLFPLHAWLPDAYTEAPTPTSALLSGVLSKAGAYGLLRFGVLMFPEAARLFAPYILTLGVIAVIYAAIIALAQT
DMKRVIAYSSFSHMGVIAVGLFTLTPEGIDGAIFTMLSHGVIITALFFCVAAVSWRAETRSIDAFSGVAHKMPVLATLAM
LFVMANIGLPGTGSFVGELLVMVGAIHVSFWVAFLAGSTMILGAVYMLVLYRRVMFGAAGHGVVAMLRDLTGPELAVLVP
CALVTLWMGIHPTSFLRVFDPAVVHAVAAPAQASAAAPIHLAAR

Sequences:

>Translated_524_residues
MGWSRRPNGDRLPPSFTDDETDVHPILLPLVTYLPLGGALAILLMRGTSEAVEGASRWVALWTSLIVFGLSVVMWGEFDP
SLPGFQFVQQVGWASSFGISYHAGVDGISLLFVLLSTFLTPLAVIGGWKLVTTRVRDYMLAVLLLETTLVGLFSALDLVL
FYVFYEATLIPGSLMIGVWGGPKRVWASIQFFLFTFGGSLFMLLALLTMWNMTGTTDIPTLMQATFPRAMQCWLLLGFVL
AFGVKLPLFPLHAWLPDAYTEAPTPTSALLSGVLSKAGAYGLLRFGVLMFPEAARLFAPYILTLGVIAVIYAAIIALAQT
DMKRVIAYSSFSHMGVIAVGLFTLTPEGIDGAIFTMLSHGVIITALFFCVAAVSWRAETRSIDAFSGVAHKMPVLATLAM
LFVMANIGLPGTGSFVGELLVMVGAIHVSFWVAFLAGSTMILGAVYMLVLYRRVMFGAAGHGVVAMLRDLTGPELAVLVP
CALVTLWMGIHPTSFLRVFDPAVVHAVAAPAQASAAAPIHLAAR
>Mature_523_residues
GWSRRPNGDRLPPSFTDDETDVHPILLPLVTYLPLGGALAILLMRGTSEAVEGASRWVALWTSLIVFGLSVVMWGEFDPS
LPGFQFVQQVGWASSFGISYHAGVDGISLLFVLLSTFLTPLAVIGGWKLVTTRVRDYMLAVLLLETTLVGLFSALDLVLF
YVFYEATLIPGSLMIGVWGGPKRVWASIQFFLFTFGGSLFMLLALLTMWNMTGTTDIPTLMQATFPRAMQCWLLLGFVLA
FGVKLPLFPLHAWLPDAYTEAPTPTSALLSGVLSKAGAYGLLRFGVLMFPEAARLFAPYILTLGVIAVIYAAIIALAQTD
MKRVIAYSSFSHMGVIAVGLFTLTPEGIDGAIFTMLSHGVIITALFFCVAAVSWRAETRSIDAFSGVAHKMPVLATLAML
FVMANIGLPGTGSFVGELLVMVGAIHVSFWVAFLAGSTMILGAVYMLVLYRRVMFGAAGHGVVAMLRDLTGPELAVLVPC
ALVTLWMGIHPTSFLRVFDPAVVHAVAAPAQASAAAPIHLAAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=340, Percent_Identity=34.1176470588235, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI251831117, Length=235, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=496, Percent_Identity=34.2741935483871, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1788831, Length=290, Percent_Identity=28.9655172413793, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788827, Length=271, Percent_Identity=26.1992619926199, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693160, Length=384, Percent_Identity=26.5625, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788614, Length=441, Percent_Identity=28.1179138321995, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI2367154, Length=253, Percent_Identity=29.6442687747036, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788829, Length=351, Percent_Identity=25.3561253561254, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56334; Mature: 56203

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWSRRPNGDRLPPSFTDDETDVHPILLPLVTYLPLGGALAILLMRGTSEAVEGASRWVA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHH
LWTSLIVFGLSVVMWGEFDPSLPGFQFVQQVGWASSFGISYHAGVDGISLLFVLLSTFLT
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHH
PLAVIGGWKLVTTRVRDYMLAVLLLETTLVGLFSALDLVLFYVFYEATLIPGSLMIGVWG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GPKRVWASIQFFLFTFGGSLFMLLALLTMWNMTGTTDIPTLMQATFPRAMQCWLLLGFVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFGVKLPLFPLHAWLPDAYTEAPTPTSALLSGVLSKAGAYGLLRFGVLMFPEAARLFAPY
HHCCCCCCHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHH
ILTLGVIAVIYAAIIALAQTDMKRVIAYSSFSHMGVIAVGLFTLTPEGIDGAIFTMLSHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VIITALFFCVAAVSWRAETRSIDAFSGVAHKMPVLATLAMLFVMANIGLPGTGSFVGELL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VMVGAIHVSFWVAFLAGSTMILGAVYMLVLYRRVMFGAAGHGVVAMLRDLTGPELAVLVP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHH
CALVTLWMGIHPTSFLRVFDPAVVHAVAAPAQASAAAPIHLAAR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC
>Mature Secondary Structure 
GWSRRPNGDRLPPSFTDDETDVHPILLPLVTYLPLGGALAILLMRGTSEAVEGASRWVA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHH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HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]