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The map label for this gene is nifU [H]

Identifier: 162146272

GI number: 162146272

Start: 443175

End: 444104

Strand: Direct

Name: nifU [H]

Synonym: GDI_0447

Alternate gene names: 162146272

Gene position: 443175-444104 (Clockwise)

Preceding gene: 162146271

Following gene: 162146273

Centisome position: 11.24

GC content: 66.24

Gene sequence:

>930_bases
ATGTGGGAATATTCCGATAAGGTAAAGGACTACTTCTTCAATCCCAAGAACGCCGGCGTGATGGAAGACGCCTCCGGCGT
GGGCGAGGTCGGCGCGATCGCCTGCGGGGATGCGCTGAAGCTGATGATCAAGGTCGATCCGCAGGATGAACGGATTACCG
AGGCCCGGTTCCAGACCTTCGGCTGCGGGTCGGCCATTGCTTCGTCCTCCGCCCTGACCGAGATCATCATCGGCAAGACG
CTGGACCAGGCGCTGGAAATCAGCAACCAGGACATCGCCGATTTCCTGGGCGGCCTGCCCCCGGAAAAGATGCACTGTTC
CGTCATGGGGTACGAGGCCCTGCGCGCGGCGGTCGCGAACTATCGCGGCGAAGTCTGGGAAGACGATCACGAGGACGGCG
CGCTGCTGTGCAGGTGCTTCGGCGTCGACGAGGGCATGGTCGAACGCGCCATCCGCAATAACGGCCTGACCAGCATGGAG
CAGGTCGCCCAGTTCACCAAGGCGACCGGCAGTTGCGGCACCTGCGTCGAAGGCGTGGAGGGCGTGCTGGAACGCACCAA
TGCGGCGATGGTGGCCGAGGGCCTTCTGGACCCGGTGCAGGCTTTCGTGCCCGGCGGTGCCGCGCCCGTCCGGGGTCGCG
CGCAGAAGACGTCACCGGTCGCACCCCCGGCGCGTACGGGCGGCAAGATGACCACCGTGCAGAAGATCCGCGCCATCGAA
GAGGTGCTGGAGGAGCTTCGCCCGGCGCTGCGCAACGACGGCGGCGACTGCGAACTGGTCGATGTCGAGGACAACCGCGT
GATGGTCCGGCTGACCGGGGCCTGCGTGAATTGCCAGCTGGCCGCCGTGACGGTGCAGGGCATCCAGGGACGTATCGCCG
AACGGCTGGGCACGCCGGTGCGTGTCATTCCGGTGCAGGCCGGGCAGTGA

Upstream 100 bases:

>100_bases
TCTCGTTCGACAATCCGAATGCGGCATCGAAATGTTCCTGCGGAAAGTCCTTTAACTGAACTGTCGCGGCAGGGTCGGGC
GTTGACGGAGGGAAATACAG

Downstream 100 bases:

>100_bases
AGGAGAGCGCGACATGAACGCGGTTTATCTGGACAACAACGCGACGACCCGCGTCGACCCGGCGGCCGTGTCCGCCATGC
TGCCGTTCTTTACCGAGCAG

Product: nitrogen fixation protein nifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MWEYSDKVKDYFFNPKNAGVMEDASGVGEVGAIACGDALKLMIKVDPQDERITEARFQTFGCGSAIASSSALTEIIIGKT
LDQALEISNQDIADFLGGLPPEKMHCSVMGYEALRAAVANYRGEVWEDDHEDGALLCRCFGVDEGMVERAIRNNGLTSME
QVAQFTKATGSCGTCVEGVEGVLERTNAAMVAEGLLDPVQAFVPGGAAPVRGRAQKTSPVAPPARTGGKMTTVQKIRAIE
EVLEELRPALRNDGGDCELVDVEDNRVMVRLTGACVNCQLAAVTVQGIQGRIAERLGTPVRVIPVQAGQ

Sequences:

>Translated_309_residues
MWEYSDKVKDYFFNPKNAGVMEDASGVGEVGAIACGDALKLMIKVDPQDERITEARFQTFGCGSAIASSSALTEIIIGKT
LDQALEISNQDIADFLGGLPPEKMHCSVMGYEALRAAVANYRGEVWEDDHEDGALLCRCFGVDEGMVERAIRNNGLTSME
QVAQFTKATGSCGTCVEGVEGVLERTNAAMVAEGLLDPVQAFVPGGAAPVRGRAQKTSPVAPPARTGGKMTTVQKIRAIE
EVLEELRPALRNDGGDCELVDVEDNRVMVRLTGACVNCQLAAVTVQGIQGRIAERLGTPVRVIPVQAGQ
>Mature_309_residues
MWEYSDKVKDYFFNPKNAGVMEDASGVGEVGAIACGDALKLMIKVDPQDERITEARFQTFGCGSAIASSSALTEIIIGKT
LDQALEISNQDIADFLGGLPPEKMHCSVMGYEALRAAVANYRGEVWEDDHEDGALLCRCFGVDEGMVERAIRNNGLTSME
QVAQFTKATGSCGTCVEGVEGVLERTNAAMVAEGLLDPVQAFVPGGAAPVRGRAQKTSPVAPPARTGGKMTTVQKIRAIE
EVLEELRPALRNDGGDCELVDVEDNRVMVRLTGACVNCQLAAVTVQGIQGRIAERLGTPVRVIPVQAGQ

Specific function: Involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=122, Percent_Identity=52.4590163934426, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI24307953, Length=123, Percent_Identity=51.219512195122, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788878, Length=125, Percent_Identity=52.8, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17543474, Length=125, Percent_Identity=49.6, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324800, Length=122, Percent_Identity=47.5409836065574, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=45.1612903225806, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355597, Length=122, Percent_Identity=48.3606557377049, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 32935; Mature: 32935

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWEYSDKVKDYFFNPKNAGVMEDASGVGEVGAIACGDALKLMIKVDPQDERITEARFQTF
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEEEECCCHHHHHHHHHHHC
GCGSAIASSSALTEIIIGKTLDQALEISNQDIADFLGGLPPEKMHCSVMGYEALRAAVAN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHEEHHHHHHHHHHHHHH
YRGEVWEDDHEDGALLCRCFGVDEGMVERAIRNNGLTSMEQVAQFTKATGSCGTCVEGVE
HCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHH
GVLERTNAAMVAEGLLDPVQAFVPGGAAPVRGRAQKTSPVAPPARTGGKMTTVQKIRAIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EVLEELRPALRNDGGDCELVDVEDNRVMVRLTGACVNCQLAAVTVQGIQGRIAERLGTPV
HHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCEECEEEEEEEHHHHHHHHHHHHCCCE
RVIPVQAGQ
EEEEECCCC
>Mature Secondary Structure
MWEYSDKVKDYFFNPKNAGVMEDASGVGEVGAIACGDALKLMIKVDPQDERITEARFQTF
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEEEECCCHHHHHHHHHHHC
GCGSAIASSSALTEIIIGKTLDQALEISNQDIADFLGGLPPEKMHCSVMGYEALRAAVAN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHEEHHHHHHHHHHHHHH
YRGEVWEDDHEDGALLCRCFGVDEGMVERAIRNNGLTSMEQVAQFTKATGSCGTCVEGVE
HCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHH
GVLERTNAAMVAEGLLDPVQAFVPGGAAPVRGRAQKTSPVAPPARTGGKMTTVQKIRAIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EVLEELRPALRNDGGDCELVDVEDNRVMVRLTGACVNCQLAAVTVQGIQGRIAERLGTPV
HHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCEECEEEEEEEHHHHHHHHHHHHCCCE
RVIPVQAGQ
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 7947754; 10819462 [H]